LOCUS       sequence-477065-       14611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant RNA Vision constructs with no gRNAs together with RFP input.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14611)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 14611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB089
FEATURES             Location/Qualifiers
     source          1..14611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5..22)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5..21)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..36)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    224..248
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1549..2178
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2607..3680
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3746..3940
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4176..4198)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4284..4424
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4339..4358
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4439..4456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4610..5198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4690..4709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5285..6079)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6504..6528
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6606..6780
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6837..7634)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6948..6967)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7558..7577
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7697..8373)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8564..8585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8600..8605,8606..8623,8624..8630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8638..8654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8643..8665
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8662..8678
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8662..8678
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8716..9061
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9216..9244)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     terminator      9398..9646
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        10282..10627
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     11624..11644
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      11689..11937
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        12582..12927
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             13052..13729
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      13731..13979
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
ORIGIN
        1 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
       61 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      121 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
      181 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
      241 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
      301 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
      361 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
      421 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
      481 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
      541 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
      601 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
      661 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
      721 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
      781 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
      841 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
      901 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
      961 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     1021 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     1081 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     1141 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     1201 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     1261 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     1321 cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc
     1381 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     1441 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     1501 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     1561 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     1621 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     1681 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     1741 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     1801 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     1861 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     1921 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     1981 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     2041 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     2101 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     2161 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     2221 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     2281 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     2341 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     2401 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     2461 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     2521 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     2581 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     2641 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     2701 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     2761 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     2821 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     2881 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     2941 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     3001 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     3061 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     3121 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     3181 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     3241 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     3301 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     3361 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     3421 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     3481 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     3541 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     3601 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     3661 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     3721 aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     3781 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     3841 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     3901 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     3961 cccaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     4021 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     4081 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     4141 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggaa
     4201 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4261 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     4321 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4381 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggccctct tccgcttcct
     4441 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4501 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5041 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5161 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5221 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
     5281 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
     5341 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
     5401 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
     5461 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
     5521 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
     5581 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
     5641 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
     5701 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
     5761 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
     5821 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
     5881 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
     5941 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
     6001 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
     6061 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
     6121 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
     6181 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
     6241 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
     6301 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
     6361 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
     6421 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
     6481 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
     6541 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
     6601 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
     6661 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
     6721 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
     6781 ctcgagcttg tcgatcgact ctagctagag gatcgatccg aaccccagag tcccgctcag
     6841 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     6901 taaagcacga ggaagcggtc agcccattcg ccgccaagtt cttcagcaat atcacgggta
     6961 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     7021 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg tgtcacgacg
     7081 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     7141 ccctgatgtt cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     7201 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     7261 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     7321 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     7381 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     7441 gcctcgtcct ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     7501 cgcccctgcg ctgacagccg aaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     7561 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     7621 tgttcaatcc ccatggtcga tcgacagatc tgcgaaagct cgagagagat agatttgtag
     7681 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
     7741 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
     7801 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     7861 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     7921 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     7981 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     8041 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     8101 gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct ttgaagacgt ggttggaacg
     8161 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
     8221 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
     8281 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
     8341 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
     8401 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct
     8461 agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8521 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8581 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8641 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat tcgagctcgg
     8701 tacccgggga tcggctgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt
     8761 ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta
     8821 caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg
     8881 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
     8941 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
     9001 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac
     9061 acgggggact caaacattac tcattcacaa aaccatctta aagcaactac acaagtcttg
     9121 aaattttctc atattttcta tttactatat aaacttttaa tcaaatcaag attaaagtta
     9181 attaaatgag caaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     9241 atggtgatgt taatgggcac aaattttctg tccgtggaga gggtgaaggt gatgctacaa
     9301 acggaaaact cacccttaaa tttatttgca ctactggaaa actacctgtt ccgtggccaa
     9361 cacttgtcac tactctgacc taaatagcaa ggatcaatat gaagatgaag atgaaatatt
     9421 tggtgtgtca aataaaaagc ttgtgtgctt aagtttgtgt ttttttcttg gcttgttgtg
     9481 ttatgaattt gtggcttttt ctaatattaa atgaatgtaa gatctcatta taatgaataa
     9541 acaaatgttt ctataatcca ttgtgaatgt tttgttggat ctcttctgca gcatataact
     9601 actgtatgtg ctatggtatg gactatggaa tatgattaaa gataagatgg gctcatagag
     9661 taaaacgagg cgagggacct ataaacctcc cttcatcatg ctatttcatg atctatttta
     9721 taaaataaag atgtagaaaa aagtaagcgt aataaccgca aaacaaatga tttaaaacat
     9781 ggcacataat gaggagatta agttcggttt acgtttattt tagtactaat tgtaacgtga
     9841 gactacgtat cgggaatcgc ctaattaaag cattaatgcg aacctgatta gattcaccga
     9901 ccctcctatc gtgtcgacct ttctgtttct tagaattttt tggtagtcta tgtactaata
     9961 atgtcagctt cgtatttatt tcataagcaa tttgcatttg caatttgttt tttactttta
    10021 tttttattgt attgtggaat gtggactcgt accaacatga agttatatac caccaaaaaa
    10081 attacagtta gtcaaaagat tcacgagtga gagctactta tgattgtctt ttacgtatat
    10141 gtctaattgt ctatttgctc aataatcttt gtactttctt ttgtcgttga taaaatcaca
    10201 aagttccaaa agtaatcgaa tgatttgctt ttaagaaaag aagagctcaa taattcaaca
    10261 tatatctgta cacagacgga ctgagacttt tcaacaaagg gtaatatccg gaaacctcct
    10321 cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg
    10381 ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga
    10441 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
    10501 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
    10561 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
    10621 gagaacacgg gggactctag acaaacatta ctcattcaca aaaccatctt aaagcaacta
    10681 cacaagtctt gaaattttct catattttct atttactata taaactttta atcaaatcaa
    10741 gattaaagtt aattaatgat cctagttacc tttgggtcaa aaagttatca ggcatgcacc
    10801 tggtagctag tctttaaacc aatagattgc atcggtttaa aaggcaagac cgtcaaattg
    10861 cgggaaaggg gtcaacagcc gttcagtacc aagtctcagg ggaaactttg agatggcctt
    10921 gcaaagggta tggtaataag ctgacggaca tggtcctaac cacgcagcca agtcctaagt
    10981 caacagatct tctgttgata tggatgcagt tcacagacta aatgtcggtc ggggaagatg
    11041 tattcttctc ataagatata gtcggacctc tccttaatgg gagctagcgg atgaagtgat
    11101 gcaacactgg agccgctggg aactaatttg tatgcgaaag tatattgatt agttttggag
    11161 tactcgatgg tgttcaatgc ttttcccgtt atccggatca catgaaacgg catgactttt
    11221 tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc aaagatgacg
    11281 ggacctacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg
    11341 agttaaaggg tattgatttt aaagaagatg gaaacattct tggacacaaa ctcgagtaca
    11401 actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta
    11461 acttcaaaat tcgccacaac gttgaagatg gttccgttca actagcagac cattatcaac
    11521 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcgacac
    11581 aatctgtcct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg
    11641 taactgctgc tgggattaca catggcatgg atgagctcta caaataaata tgaagatgaa
    11701 gatgaaatat ttggtgtgtc aaataaaaag cttgtgtgct taagtttgtg tttttttctt
    11761 ggcttgttgt gttatgaatt tgtggctttt tctaatatta aatgaatgta agatctcatt
    11821 ataatgaata aacaaatgtt tctataatcc attgtgaatg ttttgttgga tctcttctgc
    11881 agcatataac tactgtatgt gctatggtat ggactatgga atatgattaa agataagatg
    11941 ggctcataga gtaaaacgag gcgagggacc tataaacctc ccttcatcat gctatttcat
    12001 gatctatttt ataaaataaa gatgtagaaa aaagtaagcg taataaccgc aaaacaaatg
    12061 atttaaaaca tggcacataa tgaggagatt aagttcggtt tacgtttatt ttagtactaa
    12121 ttgtaacgtg agactacgta tcgggaatcg cctaattaaa gcattaatgc gaacctgatt
    12181 agattcaccg accctcctat cgtgtcgacc tttctgtttc ttagaatttt ttggtagtct
    12241 atgtactaat aatgtcagct tcgtatttat ttcataagca atttgcattt gcaatttgtt
    12301 ttttactttt atttttattg tattgtggaa tgtggactcg taccaacatg aagttatata
    12361 ccaccaaaaa aattacagtt agtcaaaaga ttcacgagtg agagctactt atgattgtct
    12421 tttacgtata tgtctaattg tctatttgct caataatctt tgtactttct tttgtcgttg
    12481 ataaaatcac aaagttccaa aagtaatcga atgatttgct tttaagaaaa gaagagctca
    12541 ataattcaac atatatctgt acacactatg gaggctcccg gtgagacttt tcaacaaagg
    12601 gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag
    12661 atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc
    12721 gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
    12781 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
    12841 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
    12901 ggaagttcat ttcatttgga gagaacacgg gggactcaaa cattactcat tcacaaaacc
    12961 atcttaaagc aactacacaa gtcttgaaat tttctcatat tttctattta ctatataaac
    13021 ttttaatcaa atcaagatta aagttaatta aatggcgagt agcgaagacg ttatcaaaga
    13081 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga
    13141 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa
    13201 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa
    13261 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg
    13321 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga
    13381 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc
    13441 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat
    13501 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg
    13561 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc
    13621 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat
    13681 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaaa tatgaagatg
    13741 aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg cttaagtttg tgtttttttc
    13801 ttggcttgtt gtgttatgaa tttgtggctt tttctaatat taaatgaatg taagatctca
    13861 ttataatgaa taaacaaatg tttctataat ccattgtgaa tgttttgttg gatctcttct
    13921 gcagcatata actactgtat gtgctatggt atggactatg gaatatgatt aaagataaga
    13981 tgggctcata gagtaaaacg aggcgaggga cctataaacc tcccttcatc atgctatttc
    14041 atgatctatt ttataaaata aagatgtaga aaaaagtaag cgtaataacc gcaaaacaaa
    14101 tgatttaaaa catggcacat aatgaggaga ttaagttcgg tttacgttta ttttagtact
    14161 aattgtaacg tgagactacg tatcgggaat cgcctaatta aagcattaat gcgaacctga
    14221 ttagattcac cgaccctcct atcgtgtcga cctttctgtt tcttagaatt ttttggtagt
    14281 ctatgtacta ataatgtcag cttcgtattt atttcataag caatttgcat ttgcaatttg
    14341 ttttttactt ttatttttat tgtattgtgg aatgtggact cgtaccaaca tgaagttata
    14401 taccaccaaa aaaattacag ttagtcaaaa gattcacgag tgagagctac ttatgattgt
    14461 cttttacgta tatgtctaat tgtctatttg ctcaataatc tttgtacttt cttttgtcgt
    14521 tgataaaatc acaaagttcc aaaagtaatc gaatgatttg cttttaagaa aagaagagct
    14581 caataattca acatatatct gtacacaagt g
//
