LOCUS       sequence-477067-       14629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of deactivated ribozyme split GFPuv with Triplex 
            motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14629)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 14629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB113
FEATURES             Location/Qualifiers
     source          1..14629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2897..3145
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3834..3850)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3834..3850)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3847..3869)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3858..3874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3882..3888,3889..3906,3907..3912))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3927..3948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4139..4815
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4883..5680
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4940..4959)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5550..5569
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5720..5894
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5972..5996
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6421..7215
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7302..7890
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7791..7810
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8044..8061
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8076..8216
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8142..8161)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8302..8324
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8560..8754
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8820..9893)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10322..10951)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12252..12276
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(12298..12550)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12582..12599)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12606..14423,14614..14628))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14424..14613
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctataaaagt tatcaggcat gcacctggta
     1981 gctagtcttt aaaccaatag attgcatcgg tttaaaaggc aagaccgtca aattgcggga
     2041 aaggggtcaa cagccgttca gtaccaagtc tcaggggaaa ctttgagatg gccttgcaaa
     2101 gggtatggta ataagctgac ggacatggtc ctaaccacgc agccaagtcc taagtcaaca
     2161 gatcttctgt tgatatggat gcagttcaca aactaaatgt cggtcgggga agatgtattc
     2221 ttctcataag atatagtcgg acctctcctt aatgggagct agcggatgaa gtgatgcaac
     2281 actggagccg ctgggaacta atttgtatgc gaaagtatat tgattagttt tggagtactc
     2341 gcttcatgct gcaacgaacg gaggttacac caacaccagg aaggagatct atgaagacgg
     2401 cggcatcttg gaggtcaact tccgttacac ttacgagttc aacaagatca tcggtgacgt
     2461 cgagtgcatt ggacatggat tcccaagtca gagtccgatc ttcaaggaca cgatcgtgaa
     2521 gagttgtccc acggtggacc tgatgttgcc aatgtccggg aacatcatcg ccagctccta
     2581 cgcttacgcc ttccaactga aggacggctc tttctacacg gcagaagtca agaacaacat
     2641 agacttcaag aatccaatcc acgagtcctt ctcgaagtca gggcccatgt tcacccacag
     2701 acgtgtcgag gagactctca ccaaggagaa ccttgccata gtggagtacc agcaggtttt
     2761 caacagcgcc ccaagagaca tgtaggattc gtcagtaggg ttgtaaaggt ttttcttttc
     2821 ctgagaaaac aaccttttgt tttctcaggt tttgcttttt ggcctttccc tagctttaaa
     2881 aaaaaaaaag caaaaatatg aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct
     2941 tgtgtgctta agtttgtgtt tttttcttgg cttgttgtgt tatgaatttg tggctttttc
     3001 taatattaaa tgaatgtaag atctcattat aatgaataaa caaatgtttc tataatccat
     3061 tgtgaatgtt ttgttggatc tcttctgcag catataacta ctgtatgtgc tatggtatgg
     3121 actatggaat atgattaaag ataagatggg ctcatagagt aaaacgaggc gagggaccta
     3181 taaacctccc ttcatcatgc tatttcatga tctattttat aaaataaaga tgtagaaaaa
     3241 agtaagcgta ataaccgcaa aacaaatgat ttaaaacatg gcacataatg aggagattaa
     3301 gttcggttta cgtttatttt agtactaatt gtaacgtgag actacgtatc gggaatcgcc
     3361 taattaaagc attaatgcga acctgattag attcaccgac cctcctatcg tgtcgacctt
     3421 tctgtttctt agaatttttt ggtagtctat gtactaataa tgtcagcttc gtatttattt
     3481 cataagcaat ttgcatttgc aatttgtttt ttacttttat ttttattgta ttgtggaatg
     3541 tggactcgta ccaacatgaa gttatatacc accaaaaaaa ttacagttag tcaaaagatt
     3601 cacgagtgag agctacttat gattgtcttt tacgtatatg tctaattgtc tatttgctca
     3661 ataatctttg tactttcttt tgtcgttgat aaaatcacaa agttccaaaa gtaatcgaat
     3721 gatttgcttt taagaaaaga agagctcaat aattcaacat atatctgtac acaattaccg
     3781 cctttgagtg agctctagat gccacctgac gtctaagaag aattcgtaat catgtcatag
     3841 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     3901 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     3961 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     4021 cgcgcgggga gaggcggttt gcgtattggc tagagcagct tgccaacatg gtggagcacg
     4081 acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg
     4141 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
     4201 gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg
     4261 ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc
     4321 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     4381 attgatgtga acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
     4441 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     4501 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
     4561 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
     4621 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     4681 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     4741 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     4801 gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc gagctttcgc
     4861 agatcccggg gggcaatgag atatggggat tgaacaagat ggattgcacg caggttctcc
     4921 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4981 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     5041 cctgtccggt gccctgaatg aactccagga cgaggcagcg cggctatcgt ggctggccac
     5101 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     5161 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     5221 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     5281 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     5341 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     5401 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacacatg gcgatgcctg
     5461 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     5521 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     5581 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     5641 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga agtagatgcc gaccggatct
     5701 gtcgatcgac aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa
     5761 ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
     5821 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta
     5881 ctaaaatcca gatcccccga attaattcgg cgttaattca gtacattaaa aacgtccgca
     5941 atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag
     6001 ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc
     6061 catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt
     6121 taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc
     6181 gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg
     6241 tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa
     6301 agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt
     6361 gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa
     6421 atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta
     6481 aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac
     6541 ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa
     6601 aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa
     6661 cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa
     6721 gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg
     6781 ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta
     6841 gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa
     6901 gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc
     6961 gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat
     7021 ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac
     7081 attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta
     7141 ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg
     7201 gatgaattgt tttagtacct agaatgcatg accaaaatcc cttaacgtga gttttcgttc
     7261 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7321 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7381 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7441 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7501 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7561 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7621 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7681 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7741 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7801 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7861 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7921 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7981 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8041 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     8101 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     8161 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     8221 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     8281 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     8341 cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg
     8401 gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg
     8461 gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc
     8521 gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt
     8581 ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt
     8641 atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac
     8701 gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag
     8761 agaccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat
     8821 taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg
     8881 ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc
     8941 ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc
     9001 gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga
     9061 aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg
     9121 ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc
     9181 ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat
     9241 accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg
     9301 tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg
     9361 cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc
     9421 ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg
     9481 taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga
     9541 agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc
     9601 acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa
     9661 tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt
     9721 tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct
     9781 tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca
     9841 tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt
     9901 cctcgggctt gggggttcca gtgccattgc agggccggca ggcaacccag ccgcttacgc
     9961 ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt
    10021 cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg
    10081 ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct
    10141 tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc
    10201 cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg
    10261 ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa
    10321 ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc
    10381 cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc
    10441 gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct
    10501 caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc
    10561 catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt
    10621 cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag
    10681 ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct
    10741 tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc
    10801 gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg
    10861 ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc
    10921 ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac
    10981 tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca
    11041 cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag
    11101 cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg
    11161 cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa
    11221 cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg
    11281 gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg
    11341 gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc
    11401 acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg
    11461 gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc
    11521 ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca
    11581 atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc
    11641 tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg
    11701 tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt
    11761 aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg
    11821 gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg
    11881 tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg
    11941 taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga
    12001 aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg
    12061 tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg
    12121 ggttggatca aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat
    12181 ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta ataaacgctc
    12241 ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aattcccgat
    12301 ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt
    12361 tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa
    12421 ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata
    12481 tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt
    12541 ttgaacgatc ggggaaattc gagctggtca ccaattcaca cgtgatggtg atggtgatgg
    12601 ctagcgttct tgtagccgaa atctggaatg ttggtccagc gctcgcgaaa gacgtgcgcg
    12661 gcgagcttcg gcttgcggtc acgagtgaac acgcccttct tgtttccttg gacgcgcatc
    12721 acgccctgag aggtcgcgaa gtccgcgaag ttccacgctt gctcacccac gaagttctca
    12781 aactcatcga acacgacgtg gttcgcctgg tagtactcga cttgatattc ctcggtgaac
    12841 atcactggat caatgtcgtg aaagcccgca acggtgtctg cgccgtactc agtgatcatg
    12901 atcggctttc ctgggcaacg cttgttccac gcgtgaaatt cctggcggag atggactttg
    12961 gccgcttcga gatcaccgcc atcgaagtac catccgttat agcgattgag cgcgatgacg
    13021 tcaatcagtt cggcgacttt gtccgtctcc ggggtagcca tcacaaacag cacgatcgtg
    13081 accggacgct tctgtgggtc gagttccttg gtcagctcca ccaacggctt gaagtactcg
    13141 tacgcgccct cttcctcagt cgccgcctcg ttggcgatgc tccacatcac gacgcttgga
    13201 tggttcttgt cacgagacac cagttcacgg agaacgtctt gatggtgctc aaacgtccga
    13261 atcttctccc aggtactgac gcgctcgctg ccttcgccga gtcccgtggt ggccatgaag
    13321 ttgaggtgca cgccaactgc cggagtctcg tcgatcacga ccagaccctc gcgatccgca
    13381 agacgcatca actcttcaga gtacggatag tgtgcggtcc ggaagctgtt ggcgccgatc
    13441 catttgagga tattgaaatc catcacattg ctcgcttcgt taaagccacg gccgttgata
    13501 ggagtgtcct catgtttgcc aaagcccttg aagtagaacg gtttgttgtt gatgaggaac
    13561 ttgccgtcgt tgacttccac ggtccgcacg ccgaacggct cttcatagac atcgatggtc
    13621 agtccgtcgt tcaccagttc cactttgatc tggtagagat acgtgttcag tggttcccag
    13681 aggatgacat tcggaatctc cacgttaccg ctcaggccct cggtgcttgc gaccactttg
    13741 ccttcctcat ccacgaccga cactttcacg gtctcggctt tgccttgaaa gtccaccgta
    13801 taggtcacag tcccggttgg gccattgaag tcggtcacaa ccgagatgtc ctcgacgtac
    13861 gtaaacgggg tcgtgtagat tttcaccgga cggtgcaggc ctgcatagtt gaagaagtcg
    13921 aagttcggct tgttacgaat gacttttccg aggccctctt cgtggcgctc gctgtacagc
    13981 cccaccggga gggtgctatc gtcgaggatg ttgtccacgg cgacggtgac gcgattcatg
    14041 ccatcacgca gcgagttgtt gatttccgct tcgaatggca ggaatccgcc cttgtgctcc
    14101 acgaccagct caccattgac atagacaatt gctttgtgag ttgcagagcc gaagcggagc
    14161 acgatacgct gatccttcag ataggccggc accgtgaact cacgttcgta ccagacatat
    14221 ccgatatggt tgcggatttc cttggtcacg ccaatgtcat tgtaactgct tgggacggcc
    14281 atactaatag tgtcggtcag cttgctttcg taccacttct cttccagtcc tttcccgtag
    14341 tccagcttga agttccagac gccattgagg tcgaagacgc cacgggtctc ggtgttgatc
    14401 gggtacagac tagttcgtcg gttctgtaac tatcatcatc atcatagaca cacgaaataa
    14461 agtaatcaga ttatcagtta aagctatgta atatttacac cataaccaat caattaaaaa
    14521 atagatcagt ttaaagaaag atcaaagctc aaaaaaataa aaagagaaaa gggtcctaac
    14581 caagaaaatg aaggagaaaa actagaaatt taccctcaga tctaccatg
//
