LOCUS       sequence-477086-        2315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BsaI Golden Gate assembly plasmid encoding the hPGK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Peterman EL, Ploessl DS, Love KS, Sanabria V, Daniels RF, Johnstone 
            CP, Godavarti DR, Kabaria SR, Oakes CG, Pai AA, Galloway KE
  TITLE     High-resolution profiling reveals coupled transcriptional and 
            translational regulation of transgenes.
  JOURNAL   Nucleic Acids Research, Volume 53, Issue 11, 24 June 2025, gkaf528
  PUBMED    39651241
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Research, Volume 53, Issue 11, 24 June 2025, gkaf528"
COMMENT     Sequence Label: pKG1367_pPV1-hPGK
FEATURES             Location/Qualifiers
     source          1..2315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      49..637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     538..557
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        672..1182
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1101..1119
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        1228..1332
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1333..1401,1402..2193)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALITFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGYRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1551..1570)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
       61 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      121 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      181 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      241 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      301 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      361 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      421 gagataccta cagcgtgagc tatgagaaag cgccacgctt ccagaaggga gaaaggcgga
      481 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      541 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      601 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg ggtctcatac
      661 tataaggatc cggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga
      721 cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg atctcgcaca
      781 ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg
      841 gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg
      901 acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga
      961 gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc
     1021 gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg
     1081 gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca
     1141 gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agaaatgaga gacctagacg
     1201 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttataaata
     1261 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1321 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattacctt ttttgcggca
     1381 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1441 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1501 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1561 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1621 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1681 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1741 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1801 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     1861 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     1921 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     1981 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     2041 gagcgtgggt atcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     2101 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     2161 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     2221 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2281 gataatctca tgaccaaaat cccttaacgt gagtt
//
