LOCUS       sequence-477096-        6529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV transfer plasmid for Syn-mediated expression of soma-targeted 
            FRCaMPi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Zhou S, Zhu Q, Eom M, Fang S, Subach OM, Ran C, Alvarado JS, 
            Sunkavalli PS, Dong Y, Wang Y, Hu J, Zhang H, Wang Z, Sun X, Yang T,
            Mu Y, Yoon YG, Guo ZV, Subach FV, Piatkevich KD
  TITLE     A sensitive soma-localized red fluorescent calcium indicator for in 
            vivo imaging of neuronal populations at single-cell resolution.
  JOURNAL   PLoS Biol. 2025 Apr 29;23(4):e3003048. doi: 
            10.1371/journal.pbio.3003048. eCollection 2025 Apr.
  PUBMED    40299972
REFERENCE   2  (bases 1 to 6529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2025-04-29"; volume: "23(4):e3003048. doi"; pages: " 
            10.1371/journal.pbio.3003048. eCollection 2025 Apr"
COMMENT     Sequence Label: pAAV-Syn-NES-SomaFRCaMPi
FEATURES             Location/Qualifiers
     source          1..6529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        349..796
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             827..853
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     1276..1295
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2927..3515
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2980..3000)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3398..3409)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3533..3740
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3792..3932
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3792..3921
                     /label=AAV2 ITR
     rep_origin      4007..4462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4094..4113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4304..4325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4479..4498)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4598..4620
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4658..4676)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4744..4848
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4849..4917,4918..5709)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5067..5086)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5880..6468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6369..6388
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc
      181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
      241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca
      301 aattaattaa tggttaatta atctagactg cagagggccc tgcgtatgag tgcaagtggg
      361 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
      421 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
      481 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
      541 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
      601 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
      661 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
      721 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
      781 gtgcctgaga gcgcagtcga gaggatccta agccaccatg cttcaacttc ctcctcttga
      841 acgtcttact ctttcgagat ctatggtgag caagggcgag gaggataaca tggccatcat
      901 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      961 gatcgagggc gagggcgagg gccgccccta cgaggccttc cagaccgcta ggctgaaggt
     1021 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     1081 ctccaaggcc tacattaagc acccagccga catccccgac tacttcaagc tgtccttccc
     1141 cgagggcttc aggtgggagc gcgtgatgag cttcgaggac ggcggcatta ttcacgttaa
     1201 tcaggactcc tccctgcagg acggcgtatt catctacaag gtgaagctgc tcggcaccaa
     1261 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cttcctacga
     1321 gatgactacc cgtaacctta cagatgagca gattgcggag ttccgtgagg ccttttcgct
     1381 gcttgatcgt gatcaggatg gaaatatcac gtccaatgaa ttgggtgtgg ttatgaggtc
     1441 gttaggtcta tcgcctactg ccgccgaatt acaagatatg attaatgagg tcgatgccga
     1501 tggtaatggc acaattgatt ttaccgaatt tttgacaatg atggcccgga aaatgaagga
     1561 taccgacgac gaagaggaag ttcgcgaagc ctttaaagtc ttcgataaag atggaagtgg
     1621 atacattaca gtcgaggagc tgactcatgt tcttacaagt ctcggtgaac gtttgtctcg
     1681 agaagaagta gccgatgtga tacgtgaagc cgactccgat ggcgatggtg taatcaacta
     1741 cgaagaattt tctcgtgtca tttcttccaa gggcggttca ggaggcagcg ggtcaggttc
     1801 aatgaggaaa ccgttccgtg gcgcgggcaa cgctgtgcgt gctttcagca cttggaaaaa
     1861 gctagtgccg cgggtttccg agtggatgta ccccgaggac ggcgccctga agagcgagat
     1921 caggaagggg ctgaggctga aggacggcgg ccactacgcc gccgaggtca agaccaccta
     1981 caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcgacatcg agttggacat
     2041 cttgtcccac aacgaggact acaccatcgt ggaacagtgc gaacgcgccg tgggccgcca
     2101 ccccaccggt ggcacggacg cgctgtacaa gggcggatct ggcggtagcg gagggacagg
     2161 tggctctgga gggagcggag gcactggcgg atctggtggg agcggaggta cctcaggccg
     2221 gactcagatc tcaagctcaa gcttcgagtt cagcagcaaa gtctcacgcg acaccctgta
     2281 cgaggcggtg cgggaagtcc tgcacgggaa ccagcgcaag cgccgcaagt ttctggagac
     2341 ggtggagctg cagatcagcc tgaagaacta cgaccctcag aaggacaaac gtttctcggg
     2401 caccgtcagg ctcaagtcca ccccacgccc caagttctcg gtgtgcgttc tgggggacca
     2461 gcagcactgt gatgaagcca aggccgtgga tatcccccac atggacatcg aggcgctcaa
     2521 gaaacttaac aaaaacaaga agttggtcaa gaagctggct aagaagtacg atgccttttt
     2581 ggcctctgag tctctgatta agcagatccc acgtatcctg ggcccaggcc taaacaaggc
     2641 tggcaagttc ccctccctgc tgacacacaa tgaaaacatg gtggccaaag tggatgaggt
     2701 gaaatcgaca atcaagttcc agatgaagaa ggtgctgtgt ttggccgtcg ctgttggcca
     2761 cgtgaagatg accgatgatg agctagtcta caacattcat ctggctgtca atttcttggt
     2821 gtccttgctt aagaaaaact ggcaaaacgt gcgggctctg tacatcaaga gcaccatggg
     2881 caagccccag cgtctgtatt aggaattccg tacaaagctt atcgataatc aacctctgga
     2941 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     3001 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     3061 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     3121 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     3181 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     3241 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     3301 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct atgttgccac
     3361 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     3421 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     3481 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccggctcg agctgtgcct
     3541 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     3601 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     3661 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     3721 aatagcaggc atgctgggga tgcggtctcg agctgatttt gtaggtaacc acgtgcggac
     3781 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     3841 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     3901 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     3961 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     4021 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4081 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4141 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4201 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     4261 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4321 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
     4381 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4441 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     4501 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     4561 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     4621 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     4681 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4741 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4801 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4861 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4921 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4981 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5041 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5101 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5161 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5221 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5281 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5341 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5401 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5461 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5521 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5581 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5641 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5701 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5761 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5821 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5881 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5941 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6001 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6061 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6121 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6181 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6241 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6301 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6361 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6421 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6481 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
