LOCUS       sequence-47721-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Lin MC, Galletta BJ, Sept D, Cooper JA
  TITLE     Overlapping and distinct functions for cofilin, coronin and Aip1 in 
            actin dynamics in vivo.
  JOURNAL   J Cell Sci. 2010 Apr 15;123(Pt 8):1329-42. Epub 2010 Mar 23.
  PUBMED    20332110
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2010-04-15"; volume: "123(Pt 8)"; pages: "1329-42. 
            Epub 2010 Mar 23"
COMMENT     Sequence Label: pBJ1807 pRS415-COF1RFP
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(717..733)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(718..734)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(758..777)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(759..777)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(787..804)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(787..803)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(796..818)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtacgagcgc tccgagggcc gccaccacct gttcctgtta ggtgctggtg acggtgctgg
       61 tgctgaccca tcttacgatg ccttcttaga gaaattgcca gaaaacgact gtctttacgc
      121 catttacgat tttgaatacg aaattaatgg taatgaaggt aagagatcca agattgtttt
      181 cttcacttgg tctccagaca ctgctccagt cagatctaag atggtctatg catcctccaa
      241 ggatgcctta agaagagcct taaacggtgt ctctaccgat gttcaaggta ctgatttttc
      301 cgaagtttct tacgattctg ttttggaaag agtcagcaga ggcgctggtt ctcattaagg
      361 taaatgattt cacaaatgac aacaatcttc aagaaaaatg aaaatgtata tctatatata
      421 aaatcgtcat atatatacag aatacatgta tagaattatt ataattataa ttttctcttc
      481 gcaactctat ctaaaacaca atacttagat ttaaggtccc gtacaccccg gctagtgggc
      541 attgtttttg cacccatatt tgtaattcta aattcagaac ggaaatactg gccatgtaga
      601 tcaagaaaag atacaaggtc atcgaaccag gcagcaatga taagggcgaa ttccagcaca
      661 ctggcggccg ttactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat
      721 accgtcgacc tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc
      781 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg tttacccaac
      841 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa ga
//
