LOCUS       sequence-477229-        5550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CDK11 p110B D543N mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Loyer P, Trembley JH, Grenet JA, Busson A, Corlu A, Zhao W, Kocak M,
            Kidd VJ, Lahti JM
  TITLE     Characterization of cyclin L1 and L2 interactions with CDK11 and 
            splicing factors: influence of cyclin L isoforms on splice site 
            selection.
  JOURNAL   J Biol Chem. 2008 Mar 21;283(12):7721-32. doi: 
            10.1074/jbc.M708188200. Epub 2008 Jan 23.
  PUBMED    18216018
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M708188200"; journalName: "J Biol Chem"; date: 
            "2008-03-21- 21"; volume: "283"; issue: "12"; pages: "7721-32"
COMMENT     Sequence Label: pUHD 10-3-human CDK11 p110B D543-->N mut. cat.-FLAG
FEATURES             Location/Qualifiers
     source          1..5550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1204..1222
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    join(1250..1268,1269..1291,1292..1310,1311..1333,1334..1352,
                     1353..1375,1376..1394,1395..1417,1418..1436,1437..1459,
                     1460..1478,1479..1501,1502..1520)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        1553..1591
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1588..1612
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4076..4099
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    4108..4242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4132..4151)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4186..4205
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4678..4697)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(4791..4808)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4962..5550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5042..5061)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     1201 aaaaataggc gtatcacgag gccctttcgt cttcactcga gtttaccact ccctatcagt
     1261 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     1321 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     1381 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     1441 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     1501 ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg
     1561 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     1621 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
     1681 ccccgaattc gctagctcgc gaactcgagg atatcgtcga ggtcatcctg tgttaactca
     1741 aatgggtgat gaaaaggact cttggaaagt gaaaacttta gatgaaattc ttcaggaaaa
     1801 gaaacgaagg aaggaacaag aggagaaagc agagataaaa cgcttaaaaa attctgatga
     1861 ccgggattcc aagcgggatt cccttgagga gggggagctg agagatcact gcatggagat
     1921 cacaataagg aactccccgt atagaagaga agactcaatg gaagacagag gagaagaaga
     1981 tgattctttg gccatcaaac caccccagca aatgtcttgg aaagaaaaag ttcatcacag
     2041 aaaagatgaa aagaggaaag aaaaatggaa gcatgctaga gtgaaagaaa gagagcacga
     2101 acgtcggaaa cgacatcgag aagaacagga taaagctcgc cgggaatggg aaagacagaa
     2161 gagaagggaa atggcaaggg agcattccag gagagaaagg gggaatgatg gcgtgtgcct
     2221 cttcagggac cgcttggagc agttagaaag gaagcgggag cgggagcgca agatgcggga
     2281 gcagcagaag gagcagcggg agcagaagga gcgcgagcgg cgggcggagg agcggcgcaa
     2341 ggagcgggag gcccgcaggg aagtgtctgc acatcaccga acgatgagag aggactacag
     2401 cgacaaagtg aaagccagcc actggagtcg cagcccgcct cggccgccgc gggagcggtt
     2461 cgagttggga gacggccgga agccagtaaa agaagagaaa atggaagaaa gggacctgct
     2521 gtccgactta caggacatca gcgacagcga gaggaagacc agctcggccg agtcctcgtc
     2581 agcagaatca ggctcaggtt ctgaggaaga agaggaggag gaggaagagg aggaggagga
     2641 agggagcacc agtgaagaat cagaggagga agaggaagag gaggaggagg agaccggcag
     2701 caactctgag gaggcatcag agcagtctgc cgaagaagta agtgaggaag aaatgagtga
     2761 agatgaagaa cgagaaaatg aaaaccacct cttggttgtt ccagagtcac ggttcgaccg
     2821 agattccggg gagagtgaag aagcagagga agaagtgggt gagggaacgc cgcagagcag
     2881 cgccctgaca gagggcgact atgtgcccga ctcccctgcc ctgttgccca tcgagctcaa
     2941 gcaggagctg cccaagtacc tgccggccct gcagggctgc cggagcgtcg aggagttcca
     3001 gtgcctgaac aggatcgagg agggcaccta tggagtggtc tacagagcaa aagacaagaa
     3061 aacagatgaa attgtggctc taaagcggct gaagatggag aaggagaagg agggcttccc
     3121 gatcacgtcc ctgagggaga tcaacaccat cctcaaggcc cagcatccca acattgtcac
     3181 cgttagagag attgtggtgg gcagcaacat ggacaagatc tacatcgtga tgaactatgt
     3241 ggagcacgac ctcaagagcc tgatggagac catgaaacag cccttcctgc caggggaggt
     3301 gaagaccctg atgatccagc tgctgcgtgg ggtgaaacac ctgcacgaca actggatcct
     3361 gcaccgtaac ctcaagacgt ccaacctgct gctgagccac gccggcatcc tcaaggtggg
     3421 tgattttggg ctggcgcggg agtacggatc ccctctgaag gcctacaccc cggtcgtggt
     3481 gacccagtgg taccgcgccc cagagctgct gcttggtgcc aaggaatact ccacggccgt
     3541 ggacatgtgg tcagtgggct gcatcttcgg ggagctgctg actcagaagc ctctgttccc
     3601 cgggaattcg gaaatcgatc agatcaacaa agtgttcaag gagctgggga cccccagtga
     3661 gaaaatctgg cccggctaca gtgagctccc agtagtcaaa aagatgacct tcagcgagca
     3721 cccctacaac aacctccgca agcgcttcgg ggctctgctc tcagaccagg gcttcgacct
     3781 catgaacaag ttcctgacct acttccccgg gaggaggatc agcgctgagg acggcctcaa
     3841 gcatgagtat ttccgcgaga cccccctccc catcgacccc tccatgttcc ccacgtggcc
     3901 cgccaagagc gagcagcagc gtgtgaagcg gggcaccagc ccgaggcccc ctgagggagg
     3961 cctgggctac agccagctgg gtgacgacga cctgaaggag acgggcttcc accttaccac
     4021 cacgaaccag ggggcctctg ccgcgggccc cggcttcagc ctcaagttca agcttgacta
     4081 caaggacgac gatgacaagg tctagaggat ccagacatga taagatacat tgatgagttt
     4141 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     4201 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     4261 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     4321 tacaaatgtg gtatggctga ttatgatcct gcaagcctcg tcgtcctggc cggaccacgc
     4381 tatctgtgca aggtccccgg ccccggacgc gcgctccatg agcagagcgc ccgccgccga
     4441 ggcgaagact cgggcggcgc cctgcccgtc ccaccaggtc aacaggcggt aaccggcctc
     4501 ttcatcggga atgcgcgcga ccttcagcat cgccggcatg tccccctggc ggacgggaag
     4561 tatccagctc gaccaagctt ggcgagattt tcaggagcta aggaagctaa aatggagaaa
     4621 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     4681 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgca ttaatgaatc
     4741 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4801 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4861 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5521 cagattacgc gcagaaaaaa aggatctcaa
//
