LOCUS       sequence-477234-        5047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Cyclin L1 alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Loyer P, Trembley JH, Grenet JA, Busson A, Corlu A, Zhao W, Kocak M,
            Kidd VJ, Lahti JM
  TITLE     Characterization of cyclin L1 and L2 interactions with CDK11 and 
            splicing factors: influence of cyclin L isoforms on splice site 
            selection.
  JOURNAL   J Biol Chem. 2008 Mar 21;283(12):7721-32. doi: 
            10.1074/jbc.M708188200. Epub 2008 Jan 23.
  PUBMED    18216018
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M708188200"; journalName: "J Biol Chem"; date: 
            "2008-03-21- 21"; volume: "283"; issue: "12"; pages: "7721-32"
COMMENT     Sequence Label: pFLEX-FLAG-human Cyclin L1 alpha
FEATURES             Location/Qualifiers
     source          1..5047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1204..1222
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1292..1312)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(1297..1626,1627..1641,1642..1910)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      1477..1612
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             1642..1910
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          1954..2050
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             2104..2127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      3684..3693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3748..3882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3772..3791)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3826..3845
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4025..4047)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4133..4273
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4188..4207
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4288..4305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4459..5047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4539..4558)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     1201 aaaaataggc gtatcacgag gccctttcgt cttcaagaat tggtcgacca attctcatgt
     1261 ttgacagctt atcatcgata agcttggctg tggaatgtgt gtcagttagg gtgtggaaag
     1321 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     1381 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     1441 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     1501 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     1561 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     1621 tgcaaaaagc tcctcgatcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     1681 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     1741 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     1801 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     1861 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctcctc
     1921 gatcgaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt
     1981 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct
     2041 ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgctct agccctcgcc
     2101 atggactaca aggacgacga tgacaagtct cgagcaattg aattcatggc gtccgggcct
     2161 cattcgacag ctactgctgc cgcagccgcc tcatcggccg ccccaagcgc gggcggctcc
     2221 agctccggga cgacgaccac gacgacgacc acgacgggag ggatcctgat cggcgatcgc
     2281 ctgtactcgg aagtttcact taccatcgac cactctctga ttccggagga gaggctctcg
     2341 cccaccccat ccatgcagga tgggctcgac ctgcccagtg agacggactt acgcatcctg
     2401 ggctgcgagc tcatccaggc cgccggcatt ctcctccggc tgccgcaggt ggcgatggca
     2461 acggggcagg tgttgtttca tcgttttttc tactccaaat ctttcgtcaa acacagtttc
     2521 gagattgttg ctatggcttg tattaatctt gcatcaaaaa tcgaagaagc acctagaaga
     2581 ataagagatt tgattaatgt attccaccac ctccgccagt taagaggaaa aaggactcca
     2641 agccccctga tccttgatca gaactacatt aacaccaaaa atcaagttat caaagcagag
     2701 aggagggtgc taaaggagtt gggattttgt gttcatgtca agcatcctca taagatcatt
     2761 gttatgtatt tacaagtctt agaatgtgaa cgtaatcaaa ccctggttca aactgcctgg
     2821 aattacatga atgacagtct tcgaaccaat gtgtttgttc gatttcaacc agagactata
     2881 gcatgtgctt gcatctacct tgcagctaga gcacttcaga ttccgttgcc aactcgtccc
     2941 cattggtttc ttcttttcgg tactacagaa gaggaaatcc aggaaatctg catagaaaca
     3001 cttaggcttt ataccagaaa aaagccaaac tatgaattac tggaaaaaga agtagaaaaa
     3061 agaaaagtag ccttacaaga agccaaatta aaagcaaagg gattgaatcc tgatggaact
     3121 ccagcccttt caaccctggg tggattttct ccagcctcca agccatcatc accaagagaa
     3181 gtaaaagctg aagagaaatc accaatctcc attaatgtga agacagtcaa aaaagaacct
     3241 gaggatagac aacaggcttc caaaagccct tacaatggtg taagaaaaga cagcaagaga
     3301 agtagaaata gcagaagtgc aagtcgatcg aggtcaagaa cacgatcacg ttctagatca
     3361 catactccaa gaagacacta taataatagg cggagtcgat ctggaacata cagctcgaga
     3421 tcaagaagca ggtcccgcag tcacagtgaa agccctcgaa gacatcataa tcatggttct
     3481 cctcacctta aggccaagca taccagagat gatttaaaaa gttcaaacag acatggtcat
     3541 aaaaggaaaa aatctcgttc tcgatctcag agcaagtctc gggatcactc agatgcagcc
     3601 aagaaacaca ggcatgaaag gggacatcat agggacaggc gtgaacgatc tcgctccttt
     3661 gagaggtccc ataaaagcaa gcaccatggt ggcagtcgct caggacatgg caggcacagg
     3721 cgctgagaat tcctgattga ttgaccggat ccagacatga taagatacat tgatgagttt
     3781 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     3841 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     3901 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     3961 tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg
     4021 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     4081 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     4141 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     4201 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     4261 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4321 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4381 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4441 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4501 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4561 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4621 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4681 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4741 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4801 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4861 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4921 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4981 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5041 atctcaa
//
