LOCUS       sequence-477257-        9568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express N-terminally FLAG tagged human USP5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
  TITLE     USP13 antagonizes gp78 to maintain functionality of a chaperone in 
            ER-associated degradation.
  JOURNAL   Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
  PUBMED    24424410
REFERENCE   2  (bases 1 to 9568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014"; volume: "3:e01369. doi"; pages: " 10.7554/eLife.01369.
            Epub 2014 Jan 14"
COMMENT     Sequence Label: pRK5-FLAG-USP5
FEATURES             Location/Qualifiers
     source          1..9568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      918..927
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             927..950
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1743..1754
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     promoter        complement(3571..3589)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3609..3626)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3615..3839
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3885..4313
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4327..4656
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4507..4642
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4723..5517
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4777..4796)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5387..5406
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5691..5812
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      5868..5877
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5881..6015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6084..6441
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6084..6275
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      6292..6427
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6461..6478)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6461..6477)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6470..6492)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6690..7145
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7162..7181)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7281..7303
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7341..7359)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7427..7531
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7532..7600,7601..8392)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7750..7769)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8563..9151
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9052..9071
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9305..9322
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9439..9460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9475..9480,9481..9498,9499..9505)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9513..9529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9518..9540
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9537..9553
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9537..9553
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc taagcttgcc gccatggact acaaggacga cgatgacaag gggtcgacaa
      961 tggcggagct gagtgaggag gcgctgctgt cagtattacc gacgatccgg gtccctaagg
     1021 ctggagaccg ggtccacaaa gacgagtgcg ccttctcctt cgacacgccg gagtctgagg
     1081 ggggcctcta catctgtatg aacacgtttc tgggctttgg gaaacagtat gtggagagac
     1141 atttcaataa gaccggccag cgagtctact tgcacctccg gcggacccgg cgcccgaaag
     1201 aggaggaccc tgctacaggc actggagacc caccccggaa gaagcccacg cggctggcta
     1261 ttggtgttga aggcgggttt gaccttagcg aggagaagtt tgaattagac gaggatgtga
     1321 agattgtcat tttgccagat tacctggaga ttgcccggga tggactgggg ggactgcctg
     1381 acattgtcag agatcgggtg accagtgcag tggaggccct actgtcggcc gactcagcct
     1441 cccgcaagca ggaggtgcag gcatgggatg gggaagtacg gcaggtgtct aagcatgcct
     1501 tcagcctcaa gcagttggac aaccctgctc gaatccctcc ctgtggctgg aagtgctcca
     1561 agtgtgacat gagagagaac ctgtggctca acctgactga tggctccatc ctctgtgggc
     1621 gacgctactt cgatggcagt gggggcaaca accacgctgt ggagcactac cgagagacag
     1681 gctacccgtt agctgtcaag ctgggcacca tcacccctga tggagctgac gtgtactcat
     1741 atgatgagga tgacatggtc ctggacccca gcctggctga gcacctgtcc cacttcggca
     1801 tcgacatgct gaagatgcag aagacagaca agacgatgac tgagttggag atagacatga
     1861 accagcggat tggtgaatgg gagctgatcc aggagtcagg tgtgccactc aagcccctgt
     1921 ttgggcctgg ctacacaggc atccggaacc tgggtaacag ctgctacctc aactctgtgg
     1981 tccaggtgct cttcagcatc cctgacttcc agaggaagta tgtggataag ctggagaaga
     2041 tcttccagaa tgccccgacg gaccctaccc aggatttcag cacccaggtg gccaagctgg
     2101 gccatggcct tctctccggg gagtattcca agccagtacc ggagtcgggc gatggggagc
     2161 gggtgccaga acagaaggaa gttcaagatg gcattgcccc tcggatgttc aaggccctca
     2221 tcggcaaggg ccaccctgaa ttctccacca accggcagca ggatgcccag gagttcttcc
     2281 ttcaccttat caacatggtg gagaggaatt gccggagctc tgaaaatcct aatgaagtgt
     2341 tccgcttctt ggtggaggaa aagatcaagt gcctggccac agagaaggtg aagtacaccc
     2401 agcgagttga ctacatcatg cagctgcctg tgcccatgga tgcagccctt aacaaagagg
     2461 agcttctgga gtacgaggag aagaagcggc aagccgaaga ggagaagatg gcactgccag
     2521 aactggttcg ggcccaggtg cccttcagct cttgcctgga ggcctacggg gcccctgagc
     2581 aggtcgatga cttctggagc acggccctgc aggccaagtc agtagctgtc aagaccacac
     2641 gatttgcctc attccctgac tacctggtca tccagatcaa gaagttcacc ttcggcttag
     2701 actgggtgcc caagaaactg gatgtgtcca tcgagatgcc agaggagctc gacatctccc
     2761 agttgagggg cacagggctg cagcccggag aggaggagct gccagacatt gccccacccc
     2821 tggtcactcc ggatgagccc aaaggtagcc ttggtttcta tggcaacgaa gacgaagact
     2881 ccttctgctc ccctcacttc tcctctccga catcgcccat gctggatgaa tcagtcatca
     2941 tccagctggt ggagatggga ttccctatgg acgcctgccg caaagctgtc tactacacgg
     3001 gcaacagcgg ggctgaggcc gccatgaact gggtcatgtc acacatggat gatccagatt
     3061 ttgcaaaccc cctcatcctg cctggctcta gtgggccggg ctccacaagc gcagcagccg
     3121 acccccctcc tgaggactgt gtgaccacca ttgtctccat gggcttctcc cgggaccagg
     3181 ccttgaaagc gctgcgggcc acgaacaata gtttagaacg ggctgtggac tggatcttca
     3241 gtcacattga cgacctggat gctgaagctg ccatggacat ctcagagggc cgctcagctg
     3301 ccgactccat ctctgagtct gtgccagtgg gacctaaagt ccgggatggt cctggaaagt
     3361 atcagctctt tgccttcatt agtcacatgg gcacctctac catgtgtggt cactacgtct
     3421 gccacatcaa gaaagaaggc agatgggtga tctacaatga ccagaaagtg tgtgcctccg
     3481 agaagccgcc caaggacctg ggctacatct acttctacca gagagtggcc agctaagcgg
     3541 ccgctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg ctagagctcg
     3601 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     3661 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     3721 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     3781 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     3841 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     3901 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3961 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4021 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4081 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4141 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4201 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4261 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     4321 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4381 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4441 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     4501 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4561 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     4621 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     4681 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     4741 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     4801 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     4861 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     4921 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     4981 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     5041 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     5101 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     5161 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     5221 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     5281 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     5341 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     5401 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     5461 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     5521 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     5581 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     5641 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     5701 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5761 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5821 gtataccgtc gaggatcccc gggtaccgag ctcgaattca atcgatggcc gccatggccc
     5881 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5941 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6001 tatcatgtct ggatcgggaa ttaattcggc gcagcaccat ggcctgaaat aacctctgaa
     6061 agaggaactt ggttaggtac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     6121 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6181 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6241 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6301 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6361 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6421 ggaggcctag gcttttgcaa aaagctgtta acagcttggc actggccgtc gttttacaac
     6481 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     6541 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     6601 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     6661 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     6721 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6781 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6841 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6901 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6961 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7021 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7081 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     7141 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     7201 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     7261 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     7321 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     7381 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7441 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7501 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7561 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7621 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     7681 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     7741 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     7801 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     7861 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     7921 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     7981 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8041 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8101 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8161 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8221 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8281 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8341 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8401 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8461 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8521 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8581 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8641 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8701 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8761 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8821 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8881 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8941 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9001 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9061 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9121 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9181 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9241 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9301 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9361 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     9421 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     9481 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     9541 aaacagctat gacatgatta cgaattaa
//
