LOCUS       sequence-477279-       14214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant expression of M. polymorpha CK2 alpha K157A, K159A,K160A, 
            K330A, H359A,K362A, tagged with TagRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14214)
  TITLE     Hothorn CK2 plasmids
REFERENCE   2  (bases 1 to 14214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 
            pMpGWB-327-Ef1-MpCKA-K157A-K159A-K160A-K330A-H359A-K362A-TagRFP
FEATURES             Location/Qualifiers
     source          1..14214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2081
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3351..3375)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3407..4102
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      4124..4376
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4409..4425)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4409..4425)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4422..4444)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4433..4449
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4457..4463,4464..4481,4482..4487))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4502..4523
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4608..4782
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(6865..7541)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6908..6928)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7736..7760
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8281..9072
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9318..9906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9807..9826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10060..10077
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10092..10232
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10158..10177)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10318..10340
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10576..10770
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10836..11909)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12338..12967)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagcaggc tttatgctac gccggttgct
     2101 tgatgtccag aaggtaggat gtcgggacgc atggaactac acagcttgct tcgtccatgt
     2161 gcgccacctt cagtctattc ccagctctct cagtgggact tccagggagg ctcacaatag
     2221 ctttctcagc ctgtccaaag tcgttgaacg agtgccgcta agctcacgaa atacagcagc
     2281 gtacgtggca tatccttctg ccaatctggc tcaaaaaata ggaaagtcga ttcggcggcc
     2341 aggtgtgatg tccaaggccc gagtttatgc agatgtcaat gtccagaggc ctaaagaata
     2401 ttgggactac gaagctctga tagttcaatg gggtgatcag gaagattacg aagtggtccg
     2461 taaggttgga cgcggcaagt acagtgaagt ttttgaaggc atcaattccg aaaacaatga
     2521 gcgctgcatc atcaagattt tgaagcctgt agccaaggct gccataaagc gagaaattaa
     2581 gattcttcaa aatctttgtg gaggaccaaa cattgtgaag cttctggata tagttcgcga
     2641 tcagcagtct aaaactccca gcctggtatt tgagtacgtg aacaatacag acttcaaagt
     2701 cctgtatccc actttgacgg attatgacat tcgttattat attctggagc ttctcaaggc
     2761 gcttgattac tgccattccc aaggaattat gcacagagac gtcaagcctc acaacgtgat
     2821 gattgaccat gagcagcgga agcttcgact tattgactgg ggtcttgctg aattttatca
     2881 tcctagcaaa gagtacaatg tccgagtagc atcaaggtac tttaaaggcc ccgagcttct
     2941 tgtggaccta caagactatg attactcttt ggacatgtgg agtctgggtt gcatgtttgc
     3001 gggaatgatc tttcggaaag agcctttttt ctacgggcat gacaaccatg atcagttggt
     3061 caagattgct gccgtcttgg gtacagatga gttgaatatg tacctgaata agtaccggtt
     3121 ggaacttgat cctcacttgg aggcacttgt aggaagggcc agtagagctc cgtggtcgaa
     3181 atttatgaat aatgacaatc agcatttggt gcttccggag gcggttgact ttttggataa
     3241 gcttcttcgg tatgaccatc aggaacggtt aactgccaga gaagcaatgc tacatcctta
     3301 cttcaatccc gtccgagcag tggaggcaaa caaccgtaat cggatcctgt acccagcttt
     3361 cttgtacaaa gtggttgata acagcgctgg aggctccggt gggagcatga gcgagctgat
     3421 taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
     3481 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     3541 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
     3601 cagcagaacc ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc
     3661 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     3721 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     3781 cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga
     3841 gatgctgtac cccgctgacg gcggcctgga aggcagaagc gacatggccc tgaagctcgt
     3901 gggcgggggc cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa
     3961 gaacctcaag atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc
     4021 cgacaaagag acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc
     4081 tagcaaactg gggcacaagt gagcttagag ctcgaatttc cccgatcgtt caaacatttg
     4141 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4201 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4261 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4321 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     4381 attggttccg gaaccaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
     4441 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     4501 aatgagtgag ctaactcaca ttaggctgaa ttaggcgcgc ctatttctga attcaacaca
     4561 ttgcggacgt ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta
     4621 gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca
     4681 aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta
     4741 attcccttat ctgggaacta ctcacacatt attatggaga aactcgagtt agtatatagt
     4801 ccttccatca ccctctgtga taatctcctt aaaacttgct cccccaggaa tcataggcag
     4861 aacgtgctcc tggtggggaa ctatcacatc cagtaaataa ggaccgggag aatctagcat
     4921 agtttgaatt gctgctctta gttcatcctt cttcaccaca cgcgcatttg gcaccttgaa
     4981 accgtctgca actgtacaga agtcggggta gatctgctcc tcgtgatctg gatcacctaa
     5041 gtaagtatgt gctctgttgg ccttgtagaa tctatcctcc aattgtacga ccattcctag
     5101 atgttggttg tttaagatca tgatcttcac gggtaacttc tccacaaaaa ttgtagccaa
     5161 ctcctgtacg ttcatcacaa agctaccatc gccatcgatg tcgatcacag gaatatctgg
     5221 ttgagttgcg gcagccccca aagcagaagg aagtccgaat cccatggctc caagacctcc
     5281 agatgtcaac caacgccgcg gttcgttgta cttgaaccac tgggctgccc acatttgatg
     5341 ttggccaaca ccagtagata tgatggcatt tccaccagtg agttcgtaca gcgtctgtat
     5401 ggcagattga ggagcaatgg catcctcgaa tacgggaaac ttcattggcc atttctgctt
     5461 ttgttcttct agttctgcca cccagttgga gaagtcgaaa gtcctctttt gactcttcaa
     5521 gagggcattg atttcactta gcgcaggttt cacatcagca cacatggaca catggggctg
     5581 tttgttcttt ccaatttcag cagggtcaat atcgatatga acgatgctgg acctgctagc
     5641 aaaagtctcc agtttaccag tcactcgatc gtcgaaacgc actccaaatg ccaacaacag
     5701 atctgcttgg tccacggcgt agttggcgta aacagttcca tgcattccaa gcattttcaa
     5761 acttaatgga tcagactcgg gatacgctcc caaacccatt aaagtcgatg tgataggaat
     5821 tccggtaagc tcgacaaatt ccctcaactc ttcgctggcg tgattgcaac ctcctccaga
     5881 atacactaca ggcttcttag aactagaaat gagtcttata atctgctcga ttaacgttgg
     5941 ttccggtggc cgaggcagtc gttgtaagta gcccttcagt tgcattggct gagaccaatc
     6001 tggaatagcc atttgctgtt gcacgtcctt gggaatatct accaagactg ggcccggcct
     6061 acctgaagct gccaaaaaga aagcctccct gattacgcga gggatgtcat caatgttcat
     6121 aacaaggtaa ttgtgcttgg tgatcgatct cgtaacctcc acaattggag tttcttgaaa
     6181 agcatcggta ccaatcatcc tactggatac ttgacctgta atggccacta gtggcacact
     6241 gtccaagagg gcatcagcga gcccggtaac aagattggtc gctccaggac cggatgtggc
     6301 tatacaaacc cctactcgtc cagacgattt agcataacct tcagcagcga aaatctcccc
     6361 ctgctcgtgg cgacagagaa tgttgcggat gctgctggaa cgtgtaagag cctggtgaat
     6421 ctccatagac gctccgccgg gataagcgaa caccgttcta atcccttctc gctcaagagc
     6481 ctccaccaaa atgtcggctc ctttgcgggg ctcgtcatcc ttgtagcgag actgaaagtc
     6541 ggacggggtt ggtgcaacga gtgacttccc gctgctaagc cgtggtccac tcacatcgac
     6601 tgcagactgg atcttctcgc taacagcttg ggctttcact tccagtgaac gctcagtctt
     6661 ccgagaggtg ttgtatgcat gtcccctcag agcggctccc gagaagaaac cactcctcac
     6721 ctttagctct ttagctccct tgcaacaaga ccttgccgat ggctgcacct tctccaggct
     6781 cgctatgctc aaaggcacag ctgcgcatcc actaattgac gccatgctcg agagagatag
     6841 atttgtagag agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta
     6901 tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
     6961 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
     7021 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
     7081 agcctttcct ttatcgcaat tatggcattt gtaggtgcca ccttcctttt ctactgtcct
     7141 tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
     7201 ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga
     7261 gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt gaagacgtgg
     7321 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
     7381 tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaattatgg catttgtagg
     7441 tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc
     7501 cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg
     7561 agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa
     7621 gctgctctag ccaatacgca aaccgaattc agaaataaat tcagcctaat tcggcgttaa
     7681 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
     7741 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
     7801 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
     7861 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
     7921 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
     7981 cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt
     8041 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     8101 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
     8161 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     8221 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
     8281 atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     8341 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     8401 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     8461 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     8521 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     8581 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     8641 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     8701 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     8761 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     8821 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     8881 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     8941 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     9001 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     9061 gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct
     9121 taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag
     9181 tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca
     9241 tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9301 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     9361 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9421 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     9481 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     9541 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9601 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9661 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9721 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9781 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9841 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9901 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9961 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    10021 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    10081 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
    10141 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
    10201 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
    10261 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
    10321 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct
    10381 tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg
    10441 cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg
    10501 gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg
    10561 cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa
    10621 gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac
    10681 cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc
    10741 tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct
    10801 gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc
    10861 tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc
    10921 aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc
    10981 ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct
    11041 gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc
    11101 aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg
    11161 tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg
    11221 atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg
    11281 gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc
    11341 aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg
    11401 tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat
    11461 tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg
    11521 gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca
    11581 gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg
    11641 cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc
    11701 ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga
    11761 tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg
    11821 gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt
    11881 accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc
    11941 cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac
    12001 acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt
    12061 tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg
    12121 atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc
    12181 ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc
    12241 aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg
    12301 tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt
    12361 cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg
    12421 ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa
    12481 tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga
    12541 tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct
    12601 taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa
    12661 tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc
    12721 cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc
    12781 ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac
    12841 tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg
    12901 ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa
    12961 ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac
    13021 cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt
    13081 ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag
    13141 gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc
    13201 gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg
    13261 cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg
    13321 gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg
    13381 ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct
    13441 ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg
    13501 agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg
    13561 gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc
    13621 atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg
    13681 ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg
    13741 tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc
    13801 tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc
    13861 gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag
    13921 cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa
    13981 gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg
    14041 gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact
    14101 gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt actttgatcc
    14161 cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa acct
//
