LOCUS       sequence-477296-        7009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent Reporter for Dopaminergic Neuron Differentiation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Oh MS, Hong SJ, Huh Y, Kim KS
  TITLE     Expression of transgenes in midbrain dopamine neurons using the 
            tyrosine hydroxylase promoter.
  JOURNAL   Gene Ther. 2009 Mar;16(3):437-40. doi: 10.1038/gt.2008.148. Epub 
            2008 Sep 18.
  PUBMED    18800154
REFERENCE   2  (bases 1 to 7009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/gt.2008.148"; journalName: "Gene Ther"; date: "2009-03"; 
            volume: "16"; issue: "3"; pages: "437-40"
COMMENT     Sequence Label: pAAV2.5-THP-dsRed2
FEATURES             Location/Qualifiers
     source          1..7009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     150..166
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     150..166
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             2784..2813
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2817..3494
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2997..3017)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3409..3432
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(3502..3519)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3508..3732
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3756..4232
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3955..3974)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4272..4412
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4272..4401
                     /label=AAV2 ITR
     rep_origin      4487..4942
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4574..4593)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4784..4805
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4959..4978)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5078..5100
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5138..5156)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5224..5328
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5329..5397,5398..6189)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5547..5566)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6360..6948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6849..6868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgggccgct ctagaactag tggatccccc aggcctcttg
      181 ggattcttct catcaaaaag ggcatgggac acagctaaag cgtccagggc tcctctgtgc
      241 ccacagatgc cttagatctt ggcacaatgt agtcagccag ctccgtgtgt gtgtgtgtgt
      301 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt ttgcatgtat
      361 ctcacagaca gtgcacaatg gcctggatgt gaacagaggc aagagtctgg gccagcagtt
      421 gtctcccagg agggtccaaa gacatcgtat tttcaagttt aggccaggtg ctcacttggg
      481 tgagctcaga cacagacaaa ggtctggaga gcacacattc cccaccccca cccagcttct
      541 atgcaagcac ctccagctga gacaagaaaa cgaattaaaa agcaatattt gtgtcagcgt
      601 aagacatttg ccgaaaggtt aaatccacac tcgtggtgct gcacagcagc cccctgtgca
      661 ggatttgtta ggcacagctc cctcctaccc cgtgccacct gagcaaatgc caggctgggt
      721 gggctggaac cagcctgggc ttgcctcacc ctggaatccc cagcaccctc caaaggagga
      781 ccctgggagt gggcatagac gcccttcagg tgtgggcaac agcccccagt cctcaggatg
      841 aaaggctaag gtgcagccag ctctgccttc acggtgggaa tgtctctatg tgagcccttt
      901 ctgggctgtg aagaacgctc tgagaagggt cctgggaccc tggataggcc agagctgtgc
      961 tgggcatgta gagacaggag tgggctaaag cagcaaaggc actgaccaag gaagagttca
     1021 gagaggagcg tggaatatgg ggaggggttc atagtaagag agagcaggca gtggagagta
     1081 aatagtcact gagccggggt ttatggggtt tgtaggagct tactcagaga aagtagatga
     1141 gagatgccat gccagtctga gtatcacaga gccccaggct ctcctgggaa cggaactgtg
     1201 agggccagaa ggtcagcaag ggaggttagg gagagttcct tttgtactga ctcagcattt
     1261 atcctgctcc cagggggcaa tgggggccag tgagggatgc agagcaaggc agtgatgtgg
     1321 caggcagttc ctgttgtgaa agagctggga agggagcggg ctgggcctgg tacgtacagc
     1381 aggccatttc tgagggtccg agtgctgtct aggaggtgca gtgagacttc agtgatcagc
     1441 cagaacagaa gctaagcggg gtggggactg cgagttcagg cttctgggtc ttgcaaatat
     1501 ccagaatgct aaatcctcag aaccccaggg tggccatttt cagagtgggt tttgtccttt
     1561 gggcacttgt gcagactcca atatccagag ggataaggat ggtactcttc agtaccctta
     1621 gtgagaggac acttttctct gaagggcttg aatgtgccga gccattacct gaaggaagga
     1681 aatgactcca gggacatagg atgggcccag cacaactcac ctgctacaga gaaaggtccc
     1741 ctccctggtc tccttagaga tcctgtttcc ctggctgagg aagctagggt ggatctttgt
     1801 gtaagtgggt gtggatgcta actggaaaac aaaaggtcac ttactgttag acctcggggt
     1861 accatggaag agatgatcac tgagtgtgcc cttacatggg gaccagctga gaatggggct
     1921 accactagct cgagaccatg atacagggaa taagtgtgca tttgggggta gggagtggct
     1981 cagaatactc ttaaccaaag cagaggtttg ctcccacagg aaggtgaggt cagaaggcct
     2041 tagggagctg ccaggggcta gggttggcac catctcccag gctgtgtctt taaggagatg
     2101 ataatcagag ggatagaacc ttgcaaaagt gggccagtct tgggaatact atagaggaat
     2161 agccttctgg aacattctgt gtctcatagg acctgcctgg ggatccagcc ccagtgccag
     2221 cacatatacc gactggggca gtgaatagat agtacacttt gttacatggg ctggggggaa
     2281 catggcccat gtcctggagg ggactttatg acagacatcc aaaaatccag tgagagggct
     2341 tctagatttg tctccaaagg ttatagttct aacatgagcc cttaggaaat ccagcatggt
     2401 tctccctgtg tgccctggtt tggttagaga gctctagcgg tctcctgtcc cacagaatac
     2461 cagccagccc ctgccctacg tcgtgcctcg ggctgagggt gattcagagg caggtgcctg
     2521 tgacagtgga tgcaattaga tctaatggga cggaggcctt tctcgtcgcc ctcgctccat
     2581 gcccaccccc gcctccctca ggcacagcag gcgtggagag gatgcgcagg aggtaggagg
     2641 tgggggaccc agaggggctt tgacgtcagc ctggccttta aagagggcgc ctgcctggcg
     2701 agggctgtgg agacagaact cgggaccacc agaggaattc gatatcaagc ttatcggatc
     2761 ctctagacgc caccatgatg gaggagcaga agctgatctc agaggaggac ctggccatgg
     2821 cctcctccga gaacgtcatc accgagttca tgcgcttcaa ggtgcgcatg gagggcaccg
     2881 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggccaca
     2941 acaccgtgaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     3001 ccccccagtt ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact
     3061 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     3121 gcggcgtggc gaccgtgacc caggactcct ccctgcagga cggctgcttc atctacaagg
     3181 tgaagttcat cggcgtgaac ttcccctccg acggccccgt gatgcagaag aagaccatgg
     3241 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagaccc
     3301 acaaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag tccatctaca
     3361 tggccaagaa gcccgtgcag ctgcccggct actactacgt ggacgccaag ctggacatca
     3421 cctcccacaa cgaggactac accatcgtgg agcagtacga gcgcaccgag ggccgccacc
     3481 acctgttcct gtaatctaga gcctcgactg tgccttctag ttgccagcca tctgttgttt
     3541 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     3601 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     3661 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     3721 tgggctctat ggcttctgag ggtcgagaga tctacgggtg gcatccctgt gacccctccc
     3781 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
     3841 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg
     3901 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt
     3961 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg
     4021 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca
     4081 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg
     4141 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
     4201 aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac
     4261 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     4321 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     4381 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     4441 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     4501 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4561 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4621 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4681 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     4741 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4801 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
     4861 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4921 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     4981 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     5041 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     5101 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     5161 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     5221 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5281 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5341 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5401 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5461 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5521 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5581 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5641 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5701 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5761 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5821 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5881 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5941 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6001 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6061 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6121 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6181 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6241 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6301 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6361 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6421 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6481 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6541 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6601 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6661 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6721 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6781 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6841 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6901 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6961 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
