LOCUS       sequence-477303-       12937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To amplify the virus that expresses C-GFP PDZD8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12937)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 12937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pBOBI C-GFP PDZD8
FEATURES             Location/Qualifiers
     source          1..12937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(6607..6609,6610..6612,6613..7323)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6607..6609,6610..6612,6613..7323)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6652..6673)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6913..6932)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7260..7281
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          7410..7639
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     7622..7641
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    7816..8404
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7869..7889)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8287..8298)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8811..8991
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9017..9034)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9023..9247
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9293..9721
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9380..9399)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9590..9611
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9730..9750)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9774..9984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9796..9992
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9843..9978
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9905..9924
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10040..10087
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10106..10480
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10610..10731
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10647..10666)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10701..10720
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10780..10796)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10780..10796)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10793..10815)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10804..10820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10828..10834,10835..10852,10853..10858))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10873..10894
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11011..11028)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11182..11770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11262..11281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11941..12732,12733..12801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12564..12583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12802..12906)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagaattcgg atccatgggg ctgctgctca tgatcctggc gtcggccgtg
     3181 ctgggttcct tcctcacgct cctcgcccag ttcttcctgc tgtaccgcag acagcccgag
     3241 ccgccggcgg acgaggccgc ccgcgcgggc gagggcttcc gctacatcaa gccagtgccg
     3301 ggcctgctcc taagggagta cctttatggc ggcggccggg atgaggagcc ctccggagcg
     3361 gcccctgagg gcggcgcgac ccccaccgcg gcccccgaga cccccgcccc gccgacgcgg
     3421 gagacttgct acttcctcaa cgccaccatc ctattcctgt tccgggagtt gcgggacacc
     3481 gcgctgaccc gccgctgggt caccaagaag atcaaggtgg agttcgagga gctgctgcag
     3541 accaagacgg ccgggcgcct gctggagggg ctgagcctgc gggacgtgtt cctgggcgag
     3601 acggtgccct tcatcaagac catccggctc gtgcggccag tcgtgccctc ggccaccggg
     3661 gagcccgatg gccctgaagg ggaggcgctg cccgccgcct gccccgagga gctggccttc
     3721 gaggcggagg tggagtacaa cgggggcttc cacctggcca tcgacgtgga cctggtcttc
     3781 ggcaagtccg cctacttgtt tgtcaagctg tcccgcgtgg tgggaaggct gcgcttggtc
     3841 tttacgcgcg tgcccttcac ccactggttc ttctccttcg tggaagaccc gctgatcgac
     3901 ttcgaggtgc gctcccagtt tgaagggcgg cccatgcccc agctcacctc catcatcgtc
     3961 aaccagctca agaagatcat caagcgcaag cacaccctac cgaattacaa gatcaggttt
     4021 aagccgtttt ttccatacca gaccttgcaa ggatttgaag aagatgaaga gcatatccat
     4081 atacaacaat gggcacttac tgaaggccgt cttaaagtta cgttgttaga atgtagcagg
     4141 ttactcattt ttggatccta tgacagagag gcaaatgttc attgtacact tgagttaagc
     4201 agtagtgttt gggaagaaaa acagaggagt tctattaaga cggttgaatt aataaaagga
     4261 aatttacaaa gtgttggact tacacttcgt cttgtccagt caactgatgg gtatgctggg
     4321 cacgtcatca ttgaaactgt ggctccaaac tcgcctgctg caattgcaga tcttcagcgg
     4381 ggagatcgac ttatcgccat tggaggtgtg aaaatcacat caacactgca agtgttgaag
     4441 cttatcaagc aggctggtga ccgagtcctg gtgtactatg aaaggcctgt tggccagagt
     4501 aatcaaggtg cagtgctgca agataacttt ggccagttgg aagaaaactt tttgtcaagc
     4561 tcatgccaat cgggttatga agaggaagct gccgggttga cagtagatac tgaaagtaga
     4621 gagctggatt ctgaatttga agacttggca agtgatgtca gagcacaaaa tgagttcaaa
     4681 gatgaggcac aatcattaag tcatagtccc aaacgtgttc caacaacact ttctattaaa
     4741 ccccttggag ctatatcacc agttttaaac cgtaaattag ctgtaggaag tcacccacta
     4801 ccaccgaaaa ttcagtccaa agatggaaat aaacctccac ccctaaaaac ttctgagata
     4861 acagacccag cacaagtgtc aaaaccaacc caaggatctg ctttcaaacc acctgtgcca
     4921 ccacgaccac aagcgaaagt tcctttgcct tccgccgatg ctccaaatca ggcagaacca
     4981 gatgttctcg ttgaaaagcc agagaaggtg gtgccacctc ctcttgtaga taaatctgct
     5041 gaaaagcaag caaaaaatgt ggatgccata gacgatgcag ctgcacctaa gcaattttta
     5101 gcaaagcaag aagtggccaa agatgtcact tcagaaactt cctgccctac taaggacagt
     5161 tcggacgacc gtcaaacatg ggaatcatca gaaattcttt atcgtaataa gctaggaaaa
     5221 tggacaagaa ccagagcatc ctgtttgttt gacatagaag cctgtcacag gtacttaaac
     5281 attgcattgt ggtgcaggga tcctttcaag ttgggaggtc tcatctgttt ggggcatgtt
     5341 agtttaaaac ttgaagatgt ggctttagga tgcctagcta catcaaacac ggaatacctt
     5401 tccaaattga gactggaagc cccctcacct aaggctatag tcactagaac cgcactacgc
     5461 aatctgagta tgcaaaaggg attcaatgac aaattttgct atggtgacat tactattcac
     5521 ttcaaatatt tgaaagaagg agaatcagac caccatgtag ttactaacgt agaaaaagaa
     5581 aaagaacccc atttggttga agaagtttct gttctcccta aagaggagca atttgttgga
     5641 cagatgggtt taacagaaaa caaacacagt tttcaggata ctcagttcca gaacccaaca
     5701 tggtgtgact actgtaagaa aaaagtttgg actaaagcag cttcccagtg tatgttttgt
     5761 gcttatgttt gccataaaaa atgtcaagaa aagtgtctag ctgagacttc tgtttgtgga
     5821 gcaactgata ggcgaataga caggacactg aaaaacctta ggctggaagg acaggaaacc
     5881 ctcttaggcc tgcctcctcg tgttgatgct gaagctagca agtcagtcaa taaaacaaca
     5941 ggtttgacaa ggcatattat caatactagt tctcgtttat taaatttgcg tcaagtctct
     6001 aaaactcgcc tttctgaacc aggaaccgat ctcgtagaac cttcaccaaa acacacaccc
     6061 aacacgtcag acaacgaagg cagtgacacg gaggtctgtg gtccaaacag tccttctaaa
     6121 cggggaaaca gcacaggaat aaagttagtg agaaaagagg gtggtctgga tgacagtgtt
     6181 ttcattgcag ttaaagaaat tggtcgtgat ctgtacaggg gcttgcctac agaggaaagg
     6241 atccagaaac tagagttcat gttggataag ctacagaatg aaattgatca ggagttggaa
     6301 cacaataatt cccttgttag agaagaaaaa gagacaactg atacaaggaa aaaatcactt
     6361 ctttctgctg ccttagctaa atcaggtgaa aggctacaag ctctaacact tcttatgatt
     6421 cactacagag caggcattga agatatagaa actttagaaa gtctgtcttt agaccagcac
     6481 tccaaaaaaa taagcaagta cacagatgat acagaagaag accttgataa tgaaataagc
     6541 caactaatag actctcagcc attcagcagc atatcagatg acttatttgg cccatccgag
     6601 tctgtgatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     6661 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     6721 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     6781 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     6841 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     6901 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     6961 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     7021 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     7081 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     7141 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     7201 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     7261 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     7321 aagctcgagc catggaagct tgatatctaa ctgactgaac cggtggtacc gaggaattaa
     7381 ttcgctgtct gcgagggcca gctgttgggg tgagtactcc ctctcaaaag cgggcatgac
     7441 ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccgc
     7501 ggtgatgcct ttgagggtgg ccgcgtccat ctggtcagaa aagacaatct ttttgttgtc
     7561 aagcttgagg tgtggcaggc ttgagatctg gccatacact tgagtgacaa tgacatccac
     7621 tttgcctttc tctccacagg tgtccactcc caggtccaac tgcaggtcga gcatgcatct
     7681 agggcggcca attccgccga tcctggaaaa acatggagca atcacaagta gcaatacagc
     7741 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     7801 agtcacacct cagacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     7861 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7921 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7981 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     8041 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8101 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     8161 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     8221 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     8281 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     8341 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8401 ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     8461 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     8521 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     8581 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     8641 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     8701 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     8761 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     8821 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8881 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8941 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggccgctt
     9001 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     9061 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     9121 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     9181 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     9241 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     9301 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     9361 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     9421 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     9481 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     9541 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     9601 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     9661 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     9721 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     9781 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     9841 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     9901 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     9961 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
    10021 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
    10081 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
    10141 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
    10201 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
    10261 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
    10321 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
    10381 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
    10441 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
    10501 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
    10561 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
    10621 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    10681 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10741 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
    10801 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    10861 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    10921 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    10981 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    11041 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    11101 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    11161 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    11221 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    11281 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    11341 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    11401 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    11461 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    11521 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    11581 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    11641 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    11701 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    11761 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    11821 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    11881 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    11941 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    12001 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    12061 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    12121 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    12181 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    12241 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    12301 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    12361 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    12421 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    12481 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    12541 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    12601 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    12661 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    12721 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    12781 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    12841 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    12901 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
