LOCUS       sequence-477304-       10870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To amplify the virus that expresses C-MYC KGA F7 with 33 residues 
            mutated to alanine at the interface for interacting with PDZD8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 10870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pBOBI C-MYC KGA F7
FEATURES             Location/Qualifiers
     source          1..10870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5173..5202
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          5271..5500
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5483..5502
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5677..6265
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5730..5750)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6148..6159)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6672..6852
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6878..6895)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6884..7108
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7154..7582
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7241..7260)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7451..7472
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7591..7611)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7596..7925
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7776..7911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7838..7857
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7973..8020
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8039..8413
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8543..8664
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8580..8599)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8634..8653
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8713..8729)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8713..8729)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8726..8748)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8737..8753
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8761..8767,8768..8785,8786..8791))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8806..8827
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8944..8961)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9115..9703)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9195..9214)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9874..10665,10666..10734))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10497..10516
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10735..10839)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagaattcgg atccatgatg cggctgcgag gctcggggat gctgcgggac
     3181 ctgctcctgc ggtcgcccgc aggagtatcg gcgactctgc ggcgggcaca gcccttggtc
     3241 accctgtgcc ggcgtccccg aggcggggga cggccggccg cgggcccggc tgccgccgcg
     3301 cgactccacc cgtggtgggg cgggggcggc tggccggcgg agcccctcgc gcggggcctg
     3361 tccagctctc cttcggagat cttgcaggag ctgggcaagg ggagcacgca tccgcagccc
     3421 ggggtgtcgc cacccgctgc cccggcggcg cccggcccca aggacggccc cggggagacg
     3481 gacgcgtttg gcaacagcga gggcaaagag ctggtggcct caggtgaaaa taaaataaaa
     3541 cagggtctgt tagctagcgc ggcagatgcg ctgttcgcta caattgctga agcagcagcg
     3601 aaaatacctg ttcataaatt tattacagca ctcaaatcta caggattgcg aacgtctgat
     3661 cccaggttgg cagcgtgtat ggctatgtta agattaactc ttgcaacaac atcagatggt
     3721 gtcatgctag acaaagatct ttttaaaaaa tgtgttcaga gcaacattgc tttgttggca
     3781 gcagcatttg caagagcgtt tgtgattcct gcctttatgg cttttacctc agccattgat
     3841 gagttatatg aaagtgctaa aaagcagtct ggaggaaagg ttgcagatta tattcctcaa
     3901 ctggccaaat tcagtcccga tttgtggggt gtgtctgttt gtacagtaga tggacagagg
     3961 cattctactg gagataccaa agttcccttc tgtcttcagt cctgtgtaaa acctttgaaa
     4021 tatgccattg ctgttaatga tcttggaact gaatatgtgc atcgatatgt tggaaaagag
     4081 ccgagtggac taagattcaa caaactattt ttgaatgaag atgataaacc acataatcct
     4141 atggtaaatg ctggagcaat tgttgtgact tcactaataa agcaaggagt aaataatgct
     4201 gaaaaatttg actatgtcat gcagtttttg aataagatgg ctggtaatga atatgttgga
     4261 ttcagtaatg caacgtttca gtctgaaaga gaaagtggag atcgaaattt tgcaatagga
     4321 tattacttaa aagaaaagaa gtgttttcca gaaggcacag acatggttgg tatattagac
     4381 ttctacttcc agctgtgctc cattgaagtg acttgtgaat cagccagtgt gatggctgcg
     4441 acactggcta atggtggtgc ctgcccaatt actgctgcag cagcactgag cgctgaagca
     4501 gttcgaaata cattgagttt gatgcattcc tgtggcatgt atgacttctc agggcagttt
     4561 gctttccatg ttggtcttcc tgcaaaatct ggagttgctg ggggcattct tttagttgtc
     4621 cccaatgtta tgggtatgat gtgctggtct cctcctctgg ataagatggg caacagtgtt
     4681 aagggaattc acttttgtca cgatcttgtt tctctgtgta atttccataa ctatgataat
     4741 ttggcagccg ctgcagcaaa agctgatcct cgagcagcag gtggtgatca aagggtaaag
     4801 tcagtgataa atcttttgtt tgctgcatat actggagatg tgtctgcact tcgaagattt
     4861 gctttgtcag ctatggacat ggaacagcgg gactatgatt ctagaacagc actccatgta
     4921 gctgctgcag agggtcatgt tgaagttgtt aaatttttgc tggaagcctg caaagtaaac
     4981 cctttcccca aggacaggtg gaataacact cccatggatg aagcactgca ctttggacac
     5041 catgatgtat ttaaaattct ccaagaatac caagtccagt acacacctca aggagattct
     5101 gacaacggga aggaaaatca aacagtccat aagaatcttg atggattgtt gctcgagcca
     5161 tggaagctta tggaacaaaa actcatctca gaagaggatc tggatatcta actgactgaa
     5221 ccggtggtac cgaggaatta attcgctgtc tgcgagggcc agctgttggg gtgagtactc
     5281 cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa acgaggagga
     5341 tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga
     5401 aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct ggccatacac
     5461 ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
     5521 ctgcaggtcg agcatgcatc tagggcggcc aattccgccg atcctggaaa aacatggagc
     5581 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     5641 ggaggaggag gtgggttttc cagtcacacc tcagacaatc aacctctgga ttacaaaatt
     5701 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5761 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5821 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5881 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5941 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6001 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6061 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     6121 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6181 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6241 atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga
     6301 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6361 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     6421 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     6481 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     6541 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     6601 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     6661 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6721 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6781 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6841 aaatctctag cagggccgct ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     6901 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     6961 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     7021 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7081 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     7141 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7201 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     7261 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7321 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     7381 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     7441 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     7501 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     7561 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     7621 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7681 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7741 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     7801 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7861 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     7921 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     7981 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     8041 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     8101 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     8161 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     8221 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     8281 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     8341 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     8401 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     8461 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     8521 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     8581 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8641 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     8701 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8761 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8821 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8881 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8941 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9001 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9061 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9121 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9181 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9241 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9301 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9361 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9421 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9481 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9541 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9601 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9661 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9721 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9781 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9841 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9901 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9961 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10021 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10081 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10141 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10201 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10261 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10321 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10381 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10441 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10501 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10561 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10621 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10681 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10741 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10801 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10861 acctgacgtc
//
