LOCUS       sequence-477311-       13081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To amplify the virus that expresses C-APEX PDZD8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13081)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 13081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pBOBI C-APEX PDZD8
FEATURES             Location/Qualifiers
     source          1..13081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..813
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             831..1011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1058..1183
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2390..2506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3033..3057
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          7481..7710
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     7693..7712
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    7887..8475
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7940..7960)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8358..8369)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8882..9062
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9088..9105)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9094..9318
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9364..9792
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9451..9470)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9661..9682
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9801..9821)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9806..10135
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9986..10121
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10048..10067
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10183..10230
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10249..10623
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10753..10874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10790..10809)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10844..10863
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10923..10939)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10923..10939)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10936..10958)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10947..10963
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10971..10977,10978..10995,10996..11001))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11016..11037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11154..11171)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11325..11913)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11405..11424)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12084..12875,12876..12944))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12707..12726
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12945..13049)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
      841 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      901 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
      961 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     1021 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     1081 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     1141 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     1201 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     1261 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     1321 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1381 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1441 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     1501 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatcaact tttaaaagaa
     2401 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2461 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg ataagcttgg
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg
     3121 ttaactctag agaattcgga tccggtggcg ggatggggct gctgctcatg atcctggcgt
     3181 cggccgtgct gggttccttc ctcacgctcc tcgcccagtt cttcctgctg taccgcagac
     3241 agcccgagcc gccggcggac gaggccgccc gcgcgggcga gggcttccgc tacatcaagc
     3301 cagtgccggg cctgctccta agggagtacc tttatggcgg cggccgggat gaggagccct
     3361 ccggagcggc ccctgagggc ggcgcgaccc ccaccgcggc ccccgagacc cccgccccgc
     3421 cgacgcggga gacttgctac ttcctcaacg ccaccatcct attcctgttc cgggagttgc
     3481 gggacaccgc gctgacccgc cgctgggtca ccaagaagat caaggtggag ttcgaggagc
     3541 tgctgcagac caagacggcc gggcgcctgc tggaggggct gagcctgcgg gacgtgttcc
     3601 tgggcgagac ggtgcccttc atcaagacca tccggctcgt gcggccagtc gtgccctcgg
     3661 ccaccgggga gcccgatggc cctgaagggg aggcgctgcc cgccgcctgc cccgaggagc
     3721 tggccttcga ggcggaggtg gagtacaacg ggggcttcca cctggccatc gacgtggacc
     3781 tggtcttcgg caagtccgcc tacttgtttg tcaagctgtc ccgcgtggtg ggaaggctgc
     3841 gcttggtctt tacgcgcgtg cccttcaccc actggttctt ctccttcgtg gaagacccgc
     3901 tgatcgactt cgaggtgcgc tcccagtttg aagggcggcc catgccccag ctcacctcca
     3961 tcatcgtcaa ccagctcaag aagatcatca agcgcaagca caccctaccg aattacaaga
     4021 tcaggtttaa gccgtttttt ccataccaga ccttgcaagg atttgaagaa gatgaagagc
     4081 atatccatat acaacaatgg gcacttactg aaggccgtct taaagttacg ttgttagaat
     4141 gtagcaggtt actcattttt ggatcctatg acagagaggc aaatgttcat tgtacacttg
     4201 agttaagcag tagtgtttgg gaagaaaaac agaggagttc tattaagacg gttgaattaa
     4261 taaaaggaaa tttacaaagt gttggactta cacttcgtct tgtccagtca actgatgggt
     4321 atgctgggca cgtcatcatt gaaactgtgg ctccaaactc gcctgctgca attgcagatc
     4381 ttcagcgggg agatcgactt atcgccattg gaggtgtgaa aatcacatca acactgcaag
     4441 tgttgaagct tatcaagcag gctggtgacc gagtcctggt gtactatgaa aggcctgttg
     4501 gccagagtaa tcaaggtgca gtgctgcaag ataactttgg ccagttggaa gaaaactttt
     4561 tgtcaagctc atgccaatcg ggttatgaag aggaagctgc cgggttgaca gtagatactg
     4621 aaagtagaga gctggattct gaatttgaag acttggcaag tgatgtcaga gcacaaaatg
     4681 agttcaaaga tgaggcacaa tcattaagtc atagtcccaa acgtgttcca acaacacttt
     4741 ctattaaacc ccttggagct atatcaccag ttttaaaccg taaattagct gtaggaagtc
     4801 acccactacc accgaaaatt cagtccaaag atggaaataa acctccaccc ctaaaaactt
     4861 ctgagataac agacccagca caagtgtcaa aaccaaccca aggatctgct ttcaaaccac
     4921 ctgtgccacc acgaccacaa gcgaaagttc ctttgccttc cgccgatgct ccaaatcagg
     4981 cagaaccaga tgttctcgtt gaaaagccag agaaggtggt gccacctcct cttgtagata
     5041 aatctgctga aaagcaagca aaaaatgtgg atgccataga cgatgcagct gcacctaagc
     5101 aatttttagc aaagcaagaa gtggccaaag atgtcacttc agaaacttcc tgccctacta
     5161 aggacagttc ggacgaccgt caaacatggg aatcatcaga aattctttat cgtaataagc
     5221 taggaaaatg gacaagaacc agagcatcct gtttgtttga catagaagcc tgtcacaggt
     5281 acttaaacat tgcattgtgg tgcagggatc ctttcaagtt gggaggtctc atctgtttgg
     5341 ggcatgttag tttaaaactt gaagatgtgg ctttaggatg cctagctaca tcaaacacgg
     5401 aatacctttc caaattgaga ctggaagccc cctcacctaa ggctatagtc actagaaccg
     5461 cactacgcaa tctgagtatg caaaagggat tcaatgacaa attttgctat ggtgacatta
     5521 ctattcactt caaatatttg aaagaaggag aatcagacca ccatgtagtt actaacgtag
     5581 aaaaagaaaa agaaccccat ttggttgaag aagtttctgt tctccctaaa gaggagcaat
     5641 ttgttggaca gatgggttta acagaaaaca aacacagttt tcaggatact cagttccaga
     5701 acccaacatg gtgtgactac tgtaagaaaa aagtttggac taaagcagct tcccagtgta
     5761 tgttttgtgc ttatgtttgc cataaaaaat gtcaagaaaa gtgtctagct gagacttctg
     5821 tttgtggagc aactgatagg cgaatagaca ggacactgaa aaaccttagg ctggaaggac
     5881 aggaaaccct cttaggcctg cctcctcgtg ttgatgctga agctagcaag tcagtcaata
     5941 aaacaacagg tttgacaagg catattatca atactagttc tcgtttatta aatttgcgtc
     6001 aagtctctaa aactcgcctt tctgaaccag gaaccgatct cgtagaacct tcaccaaaac
     6061 acacacccaa cacgtcagac aacgaaggca gtgacacgga ggtctgtggt ccaaacagtc
     6121 cttctaaacg gggaaacagc acaggaataa agttagtgag aaaagagggt ggtctggatg
     6181 acagtgtttt cattgcagtt aaagaaattg gtcgtgatct gtacaggggc ttgcctacag
     6241 aggaaaggat ccagaaacta gagttcatgt tggataagct acagaatgaa attgatcagg
     6301 agttggaaca caataattcc cttgttagag aagaaaaaga gacaactgat acaaggaaaa
     6361 aatcacttct ttctgctgcc ttagctaaat caggtgaaag gctacaagct ctaacacttc
     6421 ttatgattca ctacagagca ggcattgaag atatagaaac tttagaaagt ctgtctttag
     6481 accagcactc caaaaaaata agcaagtaca cagatgatac agaagaagac cttgataatg
     6541 aaataagcca actaatagac tctcagccat tcagcagcat atcagatgac ttatttggcc
     6601 catccgagtc tgtgaagggt tcaggatcga cttcgggatc tggtgggaaa tcatacccaa
     6661 cagtgtccgc agactaccag gatgccgtgg agaaagccaa gaagaagctg agagggttta
     6721 tcgcagaaaa gaggtgcgca cctctgatgc tgagactggc tttccacagc gcaggcacct
     6781 ttgacaaggg aaccaaaaca ggcggaccct ttggaacaat caagcaccct gctgaactgg
     6841 cacatagtgc caacaatggg ctggacatcg ccgtgcggct gctggaacct ctgaaagcag
     6901 agttcccaat tctgtcctac gccgattttt atcagctggc aggagtggtc gctgtggagg
     6961 tcactggggg ccccaaggtg cctttccacc caggacggga ggacaaacca gaaccacctc
     7021 cagaggggcg cctgccagat ccgacaaagg gctccgacca tctgcgagat gtgtttggga
     7081 aagctatggg cctgactgac caggatatcg tcgcactgtc tggagggcac accattggcg
     7141 ccgctcataa ggaaaggtca ggcttcgagg gaccctggac aagcaacccc ctgattttcg
     7201 acaattctta ctttactgaa ctgctgagtg gagagaagga agggctgctg cagctgccca
     7261 gcgacaaagc cctgctgtcc gatcccgtgt tcagacctct ggtcgataag tatgcagccg
     7321 acgaggatgc ttttttcgca gattacgcag aagcacatca gaagctgtca gaactgggat
     7381 ttgccgacgc ctgactcgag ccatggaagc ttgatatcta actgactgaa ccggtggtac
     7441 cgaggaatta attcgctgtc tgcgagggcc agctgttggg gtgagtactc cctctcaaaa
     7501 gcgggcatga cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc
     7561 acctggcccg cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga aaagacaatc
     7621 tttttgttgt caagcttgag gtgtggcagg cttgagatct ggccatacac ttgagtgaca
     7681 atgacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa ctgcaggtcg
     7741 agcatgcatc tagggcggcc aattccgccg atcctggaaa aacatggagc aatcacaagt
     7801 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     7861 gtgggttttc cagtcacacc tcagacaatc aacctctgga ttacaaaatt tgtgaaagat
     7921 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7981 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     8041 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     8101 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     8161 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     8221 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     8281 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     8341 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     8401 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     8461 gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc
     8521 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     8581 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     8641 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     8701 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     8761 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     8821 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     8881 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8941 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     9001 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     9061 cagggccgct ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     9121 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     9181 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     9241 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     9301 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     9361 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     9421 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     9481 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     9541 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     9601 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     9661 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     9721 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9781 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     9841 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     9901 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9961 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    10021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
    10081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
    10141 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
    10201 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
    10261 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
    10321 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
    10381 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
    10441 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
    10501 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
    10561 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
    10621 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
    10681 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
    10741 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    10801 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10861 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
    10921 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    10981 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    11041 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    11101 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    11161 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    11221 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    11281 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    11341 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    11401 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    11461 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    11521 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    11581 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    11641 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    11701 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    11761 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    11821 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    11881 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    11941 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    12001 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    12061 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    12121 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    12181 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    12241 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    12301 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    12361 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    12421 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    12481 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    12541 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    12601 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    12661 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    12721 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    12781 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    12841 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    12901 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    12961 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    13021 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    13081 g
//
