LOCUS       sequence-477313-        7277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for transfection of cells for expression of C-MYC ES8L3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 7277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pcDNA 3.3 N-MYC ES8L3
FEATURES             Location/Qualifiers
     source          1..7277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      742..751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             751..780
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(2663..2682)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2707..2755
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2957..3385
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3044..3063)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3254..3275
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3394..3414)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3399..3728
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3579..3714
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3641..3660
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3795..4589
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3849..3868)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4459..4478
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4765..4886
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4802..4821)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4856..4875
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4935..4951)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4935..4951)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4948..4970)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4959..4975
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4983..4989,4990..5007,5008..5013))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5028..5049
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5166..5183)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5337..5925)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5417..5436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6096..6887,6888..6956))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6719..6738
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6957..7061)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7136..7155)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actgagctcc ttaaggttaa cgccaccatg gaacaaaaac tcatctcaga agaggatctg
      781 tctagagaat tcggatccat gtcccggccc agcagcagag ccatctactt gcaccggaag
      841 gaatattccc agagtatggc ctcagaacct accctcctac agcacagggt ggagcatctg
      901 atgacatgta agctggggac acagagagtc cgggagccaa aggatgccct acagaagctt
      961 caggagatgg atgcccaggg ccgagtgtgg agtcaagacc tgttcctgca ggtcagagat
     1021 ggctggctcc atctgctgga cattgagact aaggaggaac tggattctta ccgcctggac
     1081 aacatcaagg ccatagacgt agcccttaac acctgctcct ataactccat cttatctgtc
     1141 actgtgcagg agtctggctt gccaggcatc agcactttgc tgttccaatg ccaggaagtg
     1201 ggggcagagc agctgaggac aagcctgcag aaggccctgg aagaagagct agaagaaaga
     1261 cctcgatttg gagtccatca tccaagccag gacagatgga aggggcctcc tctggaaagg
     1321 ccactcccta tacaacaggc accccctctg gagcagaggt tctctccaga gcataggttt
     1381 cctccagaac agccacataa catgacctca gaacgcagca tctcgccatc ctcaaggtct
     1441 ctgacacact acccaagtgc ccgagaaccc aatggcttca ctctgcctcc tcctccgagg
     1501 cgtgctccct ctcccgagga cccagagagg gatgaggagg tgctgaacca cgtcctaaga
     1561 gacattgagc tgtttgctgg aaagctgaag gaggtccagg caagaaacag tcataagaag
     1621 acaaaacttg ggagaaaaaa gaaaaagtct aaaaatggga taacacaagc agagtacatt
     1681 gactgcttcc agaagatcaa gttgagcttc aacctcttgg ggaagctggc tttgcggatg
     1741 caggaaacaa gcgcccctga gttcgtgggc ctcatcttcc aaaccctgaa atttatccta
     1801 tctcagtgcc ctgaggctgg ccttccagcc aaagtgattt cccccctcct cacccccaaa
     1861 gcaatagacc tgctgcagtc ctgtctaagc ccacccgagg acacactctg gaagtcacta
     1921 ggcacatcct ggaccaccag ctgggctgac tggacaggca gtgaaccgcc accctaccaa
     1981 cccacattct acgatggctg gcagattcca cagccccgct ccatgatgcc cataacaaac
     2041 caggattcga tttccctccg aggttctagg atgaggagca gcctacattt ccctcgggat
     2101 gagccataca accataaccc tgaatacgag gactcaaacc tcccactctc cagccccagc
     2161 cctggcagag cagccctgaa aatgcaagtc ctgtacgagt ttgaagcaag gaatgcccag
     2221 gaactgactg tggcacaggg ggagattctg gaggttctgg atcagagcaa gcgatggtgg
     2281 ctggtgaaga acgaagcagg actgactggt tacattccca gcaatatcct ggagcccctg
     2341 ccagctggag ccccgagagg ccaccgacaa ccatccttta gggccccaat gcttcgcctg
     2401 agctcaaagc ctgaggaggt cacagcctgg ctacaagcag agaacttctc cactgtcact
     2461 gtaaggaccc tcgggtccct aatgggaagc cagctacttc acatgagacc tggggagctg
     2521 cagatgctgt gtccacagga ggctccacgg atccaggccc ggctggacgc tgtcagaagg
     2581 atgctgggga tgactcatct cgagccatgg aagcttgata tctaactgac tgaaccggtg
     2641 gtaccaaacg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     2701 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa
     2761 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     2821 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     2881 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg cagctggggc
     2941 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3001 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3061 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3121 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3181 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3241 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3301 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3361 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     3421 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3481 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3541 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3601 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3661 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3721 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     3781 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     3841 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     3901 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     3961 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4021 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4081 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4141 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4201 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4261 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4321 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     4381 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4441 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4501 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4561 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac
     4621 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4681 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4741 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     4801 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4861 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     4921 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     4981 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5041 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     5101 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     5161 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     5221 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5281 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5341 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5401 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5461 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5521 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5581 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5641 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5701 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5761 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5821 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5881 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5941 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6001 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6061 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6121 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6181 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6241 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6301 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6361 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6421 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6481 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6541 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6601 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6661 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6721 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6781 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6841 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6901 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6961 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7021 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7081 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     7141 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     7201 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     7261 tgaagaatct gcttagg
//
