LOCUS       sequence-477316-       13394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for transfection of cells for expression of C-MYC FLNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13394)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 13394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pcDNA 3.3 N-MYC FLNA
FEATURES             Location/Qualifiers
     source          1..13394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      742..751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             751..780
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(8780..8799)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8824..8872
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9074..9502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9161..9180)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9371..9392
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9511..9531)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9516..9845
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9696..9831
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9758..9777
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9912..10706
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9966..9985)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10576..10595
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10882..11003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10919..10938)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10973..10992
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11052..11068)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11052..11068)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11065..11087)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11076..11092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11100..11106,11107..11124,11125..11130))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11145..11166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11283..11300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11454..12042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11534..11553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12213..13004,13005..13073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12836..12855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13074..13178)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13253..13272)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actgagctcc ttaaggttaa cgccaccatg gaacaaaaac tcatctcaga agaggatctg
      781 tctagagaat tcggatccat gagtagctct cactcccgct gtggccagag tgcggcggtt
      841 gcgtctccgg gaggcagtat cgattcacgg gacgcggaga tgccggctac cgaaaaagac
      901 ctagcagaag atgcaccgtg gaagaaaatt cagcagaaca cattcacccg ctggtgcaat
      961 gagcacctta agtgcgtaag caagcgcatc gccaatctgc agacggacct gagcgatggg
     1021 ttgcggctca tcgcgctgct cgaggtactc agccagaaga aaatgcaccg caagcacaac
     1081 caaagaccca ctttccgcca gatgcagctc gaaaatgtgt cggtggcgct tgaattcctg
     1141 gaccgtgaga gcatcaagct cgtgtccata gacagcaagg ctattgtgga tggaaatctg
     1201 aagctgatct taggcctcat ctggaccctg atcctgcact attccatctc aatgcccatg
     1261 tgggatgagg aagaggatga ggaggccaag aagcaaacac ccaagcagag gcttctaggc
     1321 tggattcaga acaagctacc acagcttccc attaccaact tcagtcgaga ctggcagagt
     1381 ggccgggccc tgggtgctct tgttgatagc tgtgccccag gcctatgtcc tgactgggac
     1441 tcctgggatg ctagtaagcc tgtgaacaat gcacgggaag ccatgcagca ggctgatgac
     1501 tggctaggca ttcctcaggt gattacccca gaagaaattg tggaccccaa tgtagatgag
     1561 cattctgtta tgacctacct gtctcagttt cccaaggcca agctgaagcc aggggctcct
     1621 cttcggccca aactgaaccc gaagaaagcc cgagcctatg ggccaggcat cgagcctaca
     1681 ggcaatatgg tgaagaagag agcagaattc actgtggaga cccgaagtgc tggacaggga
     1741 gaagtgcttg tatatgtgga ggacccagct ggacaccagg aagaggcaaa agtgactgcc
     1801 aataatgaca agaaccgtac tttctctgtc tggtatgtcc ctgaagtgac agggactcat
     1861 aaggtgactg tgctctttgc tggccaacat attgccaaga gcccctttga ggtgtatgtg
     1921 gacaagtcac agggtgatgc cagcaaagtg actgcccagg gccctggtct ggagcccagt
     1981 ggcaatattg ccaacaagac tacctacttt gagatcttca ctgcaggagc tggcatgggt
     2041 gaggtggaag ttgtcatcca ggaccctaca ggacagaaag gcacagtgga acctcagctg
     2101 gaggccaggg gtgacagcac ctatcgctgt agctatcagc ccaccatgga gggtgtccat
     2161 acagtacatg tcaccttcgc cggtgttccc atccctcgta gcccctacac tgtcactgtt
     2221 ggccaagcct gtaacccagc tgcctgccgg gctattggta gaggccttca gcccaagggt
     2281 gttcgagtga aggaaacagc cgacttcaag gtgtacacaa agggcgctgg cagtggggag
     2341 ctaaaggtca ctgtaaaggg tcccaagggt gaggagcgtg taaagcagaa ggacttaggg
     2401 gatggtgtgt atggctttga atattaccct acaatccctg gcacatacac tgtcaccatc
     2461 acatggggtg gccagaacat tggtcgaagt ccgtttgagg tgaaggtagg cactgagtgt
     2521 ggcaatcaga aagttcgggc atggggtcct ggcctggaag gaggcattgt tggcaagtca
     2581 gcagacttcg tagtagaggc cattggtgat gatgtgggca ccttgggttt ctctgtggaa
     2641 ggtccatcac aggcaaagat tgaatgtgac gacaagggtg atggctcctg tgatgtgcgc
     2701 tattggcccc aggaggctgg cgagtatgct gttcatgtgc tgtgtaacag tgaggatatc
     2761 cgtctcagtc ctttcatggc tgacatccgt gaggcacccc aggattttca cccagacagg
     2821 gtgaaggcac gtgggcctgg attggagaag actggtgtgg ctgtcaacaa gccagcagag
     2881 ttcacagttg atgccaagca tgctgggaag gctcctcttc gagttcaagt tcaggacaat
     2941 gagggctgct ctgtggaagc gacagtcaag gacaatggca atggtactta cagctgttct
     3001 tatgtgccca gaaagccagt gaagcacaca gccatggttt cttggggagg tgtcagcatc
     3061 cccaacagtc ctttccgggt gaatgtggga gctggcagcc atccaaacaa agtcaaggtg
     3121 tatggtccag gagtggccaa gactgggctc aaggcccatg aacctaccta ctttactgtg
     3181 gattgtactg aagctggcca gggagatgtc agcattggta tcaagtgtgc ccctggagta
     3241 gtgggcccca ctgaggctga tattgacttt gatatcatcc gcaatgacaa tgacaccttc
     3301 actgtaaaat acacaccctg tggggctggc agctatacca tcatggtcct ttttgctgac
     3361 caggccacac ccaccagccc catcagagtc aaagtggagc cttctcatga tgccagcaag
     3421 gtgaaggctg agggtcctgg cctaaatcgc actggtgttg aacttggcaa acccacccat
     3481 ttcacagtca atgctaaaac tgctgggaaa ggcaagctgg atgtccaatt ctcaggactg
     3541 gctaagggag atgcagtacg ggatgtggac atcattgacc accatgataa tacctacaca
     3601 gtcaagtaca ttcctgtgca gcagggccca gtaggtgtca atgtcactta tggaggagat
     3661 cacatcccca agagtccatt ttcagtggga gtatctccaa gcctggatct cagcaaaatc
     3721 aaggtgtctg gccttggtga caaagtggac gttggcaaag atcaagagtt cacagtaaag
     3781 tcaaagggtg caggtggtca aggcaaagta gcatccaaga ttgtgagtcc ctcaggtgca
     3841 gcggtaccct gcaaggtaga gccaggcctg ggagctgaca acagcgtggt acgttttgtg
     3901 ccccgtgaag aggggcccta tgaggtggaa gtgacctatg atggtgtgcc tgtacctggc
     3961 agtccctttc cactagaagc tgtggccccc accaaaccca gcaaggtgaa ggcgtttgga
     4021 ccagggctac aggggggcaa tgcaggctcc cctgcccgct tcaccattga tacaaagggt
     4081 gctggcactg gtggcctggg cctgacagtg gaaggcccct gtgaagcaca gcttgagtgc
     4141 ctagacaacg gggatggtac atgctctgtg tcttatgtac ccactgagcc tggggactac
     4201 aacatcaaca tcctttttgc tgacacccac attcctggat ccccattcaa ggcccatgtg
     4261 gctccttgtt ttgatgcatc caaggtgaag tgctcaggcc ctgggctgga gcgggctact
     4321 gctggtgagg tagggcagtt ccaagtggac tgttcaagtg ctggcagtgc tgagttgacg
     4381 attgagatct gctctgaggc aggactgcca gctgaagtat acattcaaga ccatggtgat
     4441 ggcacacaca ccattaccta tattcctctc tgtcctgggg cttacactgt taccatcaag
     4501 tatggcggcc agcctgtgcc caacttcccc agcaagctac aggtggaacc tgctgtagat
     4561 acctcaggtg tacagtgcta tgggcctggg attgaaggtc aaggtgtctt ccgagaggca
     4621 accactgagt tcagtgtgga tgcccgggct cttacacaga ctggagggcc acatgtcaag
     4681 gctcgagtgg ccaacccctc aggcaatctg acagatacct atgtgcaaga ctgtggggat
     4741 ggcacataca aagtggaata cactccatat gaggaaggag tacactctgt ggatgtgact
     4801 tatgatggca gccctgtgcc cagcagcccc ttccaggtgc ctgtaacaga gggctgtgac
     4861 ccctcccggg tgcgtgtcca tggaccaggc atccaaagtg gtaccaccaa caaacccaac
     4921 aagttcacag tagaaaccag gggagctggc acaggtggcc tgggcttggc tgttgagggt
     4981 ccctcagagg ccaagatgtc ttgtatggat aataaagatg gcagctgctc agtagaatac
     5041 atcccctatg aagctggaac ctatagcctt aatgtcactt atggtggtca ccaagtgcca
     5101 ggtagtccct tcaaggtccc tgtacatgat gtgacagatg catctaaagt caagtgttct
     5161 ggacctggcc taagcccagg catggtccgt gccaacctcc ctcagtcctt tcaggtggac
     5221 acaagcaaag ctggagttgc cccactgcag gtcaaagtgc aggggcccaa aggcctggtg
     5281 gagccagtgg atgtagtgga caatgctgat ggtactcaga ctgtcaacta tgtgcccagc
     5341 cgagaagggt cctatagcat ttctgtgctg tatggtgaag aagaagtgcc acggagcccc
     5401 ttcaaggtca aggtgctgcc tacacatgat gccagtaagg tgaaggccag tggacctgga
     5461 ctcaacacca ctggtgtacc tgctagcctg cctgtggagt tcaccattga tgccaaggat
     5521 gctggggagg gtctgttggc tgtccagatt acggatcctg aaggcaagcc caagaagaca
     5581 cacattcaag ataatcatga tggcacatac acggtggctt atgtgccaga tgtgccaggc
     5641 cggtacacaa tcctcatcaa gtatggtggt gatgagattc ccttttcccc gtaccgtgtc
     5701 cgggctgtgc ccactgggga tgccagcaag tgcacagtca caggtgctgg cattggcccc
     5761 accatccaga ttggggagga gacggtgatt actgtggaca caaaagcagc aggcaaaggc
     5821 aaagtgactt gtactgtgtg cacacctgat ggctcagagg tagacgtgga cgtggtggag
     5881 aatgaggatg gcacctttga catcttctac acagctcccc aaccgggcaa atatgtcatc
     5941 tgtgtgcgct tcggtggcga gcatgtgccc aacagcccct tccaagttac agctttggct
     6001 ggggaccaac caacagtgca gaccccatta aggtctcagc agctggctcc acagtataac
     6061 tatcctcagg gtagccagca aacctggatt ccagagaggc ccatggtggg cgttaatggg
     6121 ctggatgtga ccagcctgag gccctttgat cttgtcatcc ccttcactat caagaagggg
     6181 gagatcactg gggaagttcg aatgccctca ggcaaggtgg cccagccttc cattactgat
     6241 aacaaagatg gcactgttac tgtacgttac tcacccagtg aagctggcct gcatgaaatg
     6301 gacattcgct atgacaatat gcatatccca ggaagccctc tgcagttcta tgttgattat
     6361 gtcaactgtg gccacatcac tgcttatggt cctggcctta cccatggagt ggtcaacaaa
     6421 cctgccacct tcactgtcaa caccaaggat gcaggagagg ggggcttgtc tctggccatt
     6481 gagggtccat ctaaagcaga aatcagttgc actgacaacc aggatggaac atgcagtgtc
     6541 tcttacctgc ctgtactgcc tggtgactat agcatcctag ttaagtacaa tgatcaacac
     6601 atcccaggca gtccctttac tgccagagta acaggtgacg attccatgcg tatgtcccac
     6661 ctaaaggtgg gttctgctgc tgatatcccc atcaatatct cagaaacaga ccttagccta
     6721 ctcacagcca ctgtggtgcc accttcgggt cgagaggaac cctgtctgct gaaacgtttg
     6781 cgaaatggcc acgtggggat ttccttcgtg cccaaggaga caggggagca cctggtacat
     6841 gtgaagaaga atggccagca tgtggcaagc agtcccatcc cagtagtgat cagccagtcg
     6901 gagataggtg atgccagccg tgtgagggtc tctggtcaag gtcttcatga aggtcatacc
     6961 tttgagcctg cagagtttat tattgacacc agagatgcag gctacggtgg gcttagtctg
     7021 tccattgagg gccctagcaa agtagacatc aacacagagg atctggagga tggcacatgc
     7081 agggtcacct actgtcccac agagcctgga aactacatta taaacatcaa atttgctgac
     7141 cagcatgtgc ctggcagtcc cttttctgtg aaggtgacag gtgagggccg ggtgaaagag
     7201 agtatcacac gcaggcgacg tgccccttct gtggccaata ttggcagtca ttgtgacctc
     7261 agcctgaaga ttcctgaaat tagcatccaa gatatgacag cccaggtgac cagcccatca
     7321 ggcaagaccc atgaggcaga gatcgtagaa ggagagaacc atacttactg tatccgattt
     7381 gtgcctgctg agatgggaat gcatacagtc agtgtcaagt acaagggcca gcatgtacct
     7441 gggagcccct tccagttcac tgtggggcct ctgggggaag ggggtgctca caaggtccgt
     7501 gctggaggcc ctggcctaga gagggctgaa gttggagtgc cagcggagtt cggcatttgg
     7561 actagggaag ctggcgctgg aggcctggcc attgctgttg aaggccccag caaggctgag
     7621 atctctttcg aggaccgaaa ggatggctcc tgtggtgtgg cctacgtagt tcaggagcca
     7681 ggtgactatg aggtctcagt caagttcaac gaggagcaca tacctgatag ccccttcgtg
     7741 gtgcctgtgg cttctccgtc tggtgacgcc cgccgcctta ctgtttctag tcttcaggag
     7801 tcagggttaa aggtcaacca gccagcatct tttgcagtca gtctgaatgg agccaagggg
     7861 gcaattgatg ccaaggtgca cagcccctca ggagctctgg aggagtgcta tgtcacagag
     7921 attgaccaag ataagtatgc tgtgcgtttc atcccacgag agaatggcat ctacttgatt
     7981 gatgtcaagt tcaatggtac tcacattcct ggaagtccct tcaagatccg agttggggag
     8041 cctgggcatg gaggggaccc aggcttagtg tccgcctatg gagcaggcct ggaaggtggt
     8101 gtcacaggga gcccagcaga gtttattgtg aacacaagca atgcaggagc tggtgccctt
     8161 tcggttacca ttgatggccc ctccaaggtg aagatggatt gccaggagtg ccccgagggc
     8221 tatcgtgtca cctatacccc catggcacct ggcagctacc tcatctccat caagtatggt
     8281 ggcccctatc acattggtgg aagtcccttt aaagccaagg tcacaggtcc tcgtcttgta
     8341 agcaaccaca gcctccatga gacatcatct gtgtttgtgg actctttgac taaagttgcc
     8401 acggttcccc agcatgcaac ctcaggccca ggtcctgctg atgtcagcaa ggtagtagcc
     8461 aaaggcctgg ggctaagcaa ggcttatgta ggccagaaga gcaacttcac agtagattgc
     8521 agcaaagcag gtaacaacat gctgctggtg ggcgtgcatg gcccaaggac accctgtgaa
     8581 gagatcctgg tgaaacacat gggcagccgc ctctatagtg tctcttacct gctcaaggac
     8641 aaaggggagt acacactggt ggtcaagtgg ggtgatgagc atatcccagg cagcccatac
     8701 cgcattatgg tacccctcga gccatggaag cttgatatct aactgactga accggtggta
     8761 ccaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     8821 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
     8881 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     8941 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     9001 ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct
     9061 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9121 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9181 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9241 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     9301 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     9361 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     9421 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     9481 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     9541 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     9601 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     9661 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     9721 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9781 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     9841 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     9901 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     9961 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
    10021 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
    10081 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
    10141 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
    10201 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
    10261 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
    10321 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
    10381 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
    10441 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
    10501 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
    10561 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
    10621 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
    10681 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa
    10741 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
    10801 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    10861 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
    10921 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    10981 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
    11041 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    11101 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    11161 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    11221 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    11281 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    11341 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    11401 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    11461 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    11521 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    11581 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    11641 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    11701 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    11761 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    11821 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    11881 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    11941 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    12001 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    12061 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    12121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    12181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    12241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    12301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    12361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    12421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    12481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    12541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    12601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    12661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    12721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    12781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    12841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    12901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    12961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    13021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    13081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    13141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    13201 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
    13261 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
    13321 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
    13381 agaatctgct tagg
//
