LOCUS       sequence-477318-        9572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for transfection of cells for expression of C-MYC pdzd-8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 9572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pcDNA 3.3 N-MYC pdzd-8
FEATURES             Location/Qualifiers
     source          1..9572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      742..751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             751..780
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4958..4977)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5002..5050
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5252..5680
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5339..5358)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5549..5570
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5689..5709)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5694..6023
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5874..6009
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5936..5955
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6090..6884
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6144..6163)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6754..6773
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7060..7181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7097..7116)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7151..7170
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7230..7246)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7230..7246)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7243..7265)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7254..7270
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7278..7284,7285..7302,7303..7308))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7323..7344
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7461..7478)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7632..8220)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7712..7731)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8391..9182,9183..9251))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9014..9033
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9252..9356)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9431..9450)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actgagctcc ttaaggttaa cgccaccatg gaacaaaaac tcatctcaga agaggatctg
      781 tctagagaat tcggatccat gttaatagca tttctagtcg ggtttatttg cggtgttctt
      841 cttctccttg gaattctgta tatatttatc gtcgacccat gggcacctcc aactgaccaa
      901 ccacaaccac ctatcgaaca attccgtccg gctcagattc cagaggaact tcgcactttt
      961 ctaaaatccg gagaagatgg acaaggaatt tcaaaatggg aatcttgcaa ttctatcagt
     1021 cttctacttc atatgctatt tcaagaacac aaggatacac gtgcattaag aagatgggtt
     1081 cacaaacgtc ttcaaatgga aatgaatgac attacaactc gatcggctgc cggacgcctc
     1141 attcaagaaa ttcgcattcg agaattgtcg cttggcacca aattcatgac catcaactcg
     1201 attcgagtag aaagtgttga aatggctgat gataaaaaca cgtttgataa aattgtgttc
     1261 attcttgaca ttgactactc gggcggattt gaaacctcaa ttgacgtctc aacaataatt
     1321 gccaaaaaag cgagcttatc cgttaaaatc acaaagttaa ctggaatggt gcgagtgatt
     1381 ttgtcgcgac aaccatatca tcactggaca ttctcgtttg ttagccaacc aatttttgaa
     1441 accgatatta actcacagat tcaagggcat caactgaaaa gattgattcc aattatcaaa
     1501 gaagcaattc gtagatcatt acaaaggaaa catgtttggc ctaattataa gatcagatac
     1561 agaccgttct tcccgaatcc aatattccag gcttcaccac caataaatag cttcactcat
     1621 atcaaaatgg aaggaggaat cgaagtaact gtacttcaat gttcacgact gaaaaatgct
     1681 cttcttcttg atgacaagaa caaaaattac gaagtttatt gtactgtttc gatagaatct
     1741 cgtccaattg ttcaaaatga agagcaggga catgttgtca atgtgttgct cactttctca
     1801 cgttatgatg tatcctctcc aattggatta gttttcgata aaaatgttgc ggccactggt
     1861 aacaatacga atcgtgctgt taaagtatgc actgtcgagg ataacagttt agcggataag
     1921 gctgctttca aaccaggaga tgtactcgtt gcaatcaata atgttccaat tcgatctgaa
     1981 cgacaagcaa cgagattttt acaatcgact actggtgatt tgacggtgct tgtggagaga
     2041 agtcttgatg atatcgatga ggaagaatcg aaagaagcag ccgaaattgt cgtgagtact
     2101 gttcgagata tcgatggaac tggtagcgtt aatgatacag ctgattctac gagtttgata
     2161 agcaataatt cggtggcaaa cgatagagat tcactgaagg atgatgataa aactattgga
     2221 ggaataccgg cgaaagatgg aaaaattaat gccaccgccc ttgccgcaag acgtcgtcac
     2281 agtgtgtcaa atttggaatc ttctgtaatc gacacgaaca ccgaatctgt tgcctcttct
     2341 attttcggat tagacgctca caaattcgat aaacctccat ctgtctctgt tcaacgtgct
     2401 gaaagtgctc gagtgcctca acttctgatc agccagccaa gtttcgattt acgaagaaca
     2461 caatcggaaa gtcatctcga tgcaaaagtc attgatgccg ttttatcgag ttctgcagat
     2521 tttggaatgg aaaattctgt ggaagacgag atcaagatgt tgttggttgg agatttgaat
     2581 agaagtgcat ctgctggaca taatctggat ccacaattac ttggagttgt taaaagagag
     2641 gataaagata aaacattgac ccaggctgat gcttcaaaag gattggcgtt gattgcttca
     2701 caaaccggtt cggttgcctc cctttcatca atgatctcta atcgtactga cactggaaca
     2761 gaagacggag gtgatgatga agaagcacat cttgacagaa aacgatctgc gagcacacga
     2821 aaagccaaaa tacaggcaac atttgctgct ggaaagaaga aagttctcga tttgatgcct
     2881 cagaaacgaa agaacaccga tgcttcagat ctcaatgggg aaagcatcga aattggagca
     2941 gatgctggtt cactttacga cacagatacg aacagagatc catcgattgg aagatctccc
     3001 atggcagtcg ttgggaaaag gaaagaaagt ccatcagatg agaaaaaaga gaaaaagaga
     3061 ttgaagaaga agttgtcaga atctccgaag atcagaagag ctgctggttc agcacagaag
     3121 gatcagtctc caatgactac aatcaaagct agaaccacaa agagtgttca atttcagaag
     3181 tttcaggacg taatgtgggg ccaatcactt cactttgaat ttgattcgcc tccagaatcc
     3241 accacacgaa ctgttattcg atacctcaac gtgactgttc acgcaaaaga agtgaaatca
     3301 gctggaacgc cgacaacttc tgccacgaat gtaccaaatg caacaccatc aactccagat
     3361 agcattgctt cttcgtcaac aaatacggat aactctgttc caacaaatat atctgcagat
     3421 tcaaaaccaa ttcttcttgg aagtgtctcg cttttcgttc ctcaattaat tgatgattgt
     3481 cgtctaacac tttcaaattg tcatcgagaa gtttatcagt tgaagcaacc aaacacgtca
     3541 tctccaccac aaacatcacc tgatgatccg atgcttgccg agtttgcccg ccatgctgga
     3601 tacgatcctc gtctttgctt cggtgacata actcttggtt ttcgttattt ccctaatgga
     3661 ttccctgttg acaaagcaat caattctggc gatgaaagtg aagatgagct gaataggata
     3721 cataattcaa aagatgtagc atcgccgacg aggcctttct caccaccagc tttatcccct
     3781 gcaagtcatg actggaagct ctggtatgga aagaattcta cgacctgcgc aatgtgtcga
     3841 ggtaaaattt ggctccgcaa cgcttcttcg tgctcacggt gtcttgtcat ttgccataac
     3901 aaatgcgtcg tcaaggccaa tagcggagga atcgcatgta caccccaaca acatctcaca
     3961 ccgccatcaa atcttcaact tcactctgac gagcattttg aagagatatc tgcttctgaa
     4021 ctagatcatc catctgtaac tccaggagat catcaaattg atgagacacg ttcattgata
     4081 atgcaatcac catctactcc ttcaagttct attgctggat cgctagacac accagaaatg
     4141 tcaaaacgcg ccagattccg aaaagtgaca gaaaagtttt caaattggag aaaaggagga
     4201 aaaaagaagg atgacgacga tttcatcgga agaagagaaa ctattgacac tgattcatgt
     4261 gctgatactg gttccttaca atctcaagac atttccaata gagatagccc aatggcaagt
     4321 attcaagatg ttctctccga tgttcttcca ggtctagatg gatctccatt cataagtgga
     4381 ttatattttc agcctggaaa tgcatataat gagcagacga ttcgaaatgc aaaaatgctt
     4441 ggacgtgaga tcttctgtga tcttccatct gagcaacgag tcgagagaat caactcacaa
     4501 atcgatagaa ttcagacagc gataagagaa acgaaagata atcgattgag tgtgatgcaa
     4561 aatggcggag acatttcaga atcgtcagcc aagtttcaag gtctggacga acgtcttcaa
     4621 gctctcgctg ttgtcatgct tcactattgt tctgctcttc aagattgcca atcaggaaga
     4681 tccacaccag ctccaccacc agatgatgat aatcagaaga gagagattac cgaagaaata
     4741 tcatccgtca acgtcgactc aaacaatgaa gaagactccg aaagtgttct agatgaggat
     4801 acagtaaccg gtgacgttcc ggtgcaacac gatcatccac ttgagcgacc aacaaagctg
     4861 gatccgagtt cggatgcagt tgaagcccat tccctcgagc catggaagct tgatatctaa
     4921 ctgactgaac cggtggtacc aaacggggga ggctaactga aacacggaag gagacaatac
     4981 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg
     5041 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     5101 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     5161 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcagat
     5221 ctgcgcagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     5281 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5341 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5401 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5461 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5521 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5581 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5641 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     5701 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5761 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5821 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5881 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5941 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     6001 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     6061 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     6121 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     6181 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     6241 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     6301 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     6361 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     6421 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     6481 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     6541 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     6601 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     6661 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6721 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     6781 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     6841 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     6901 cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     6961 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     7021 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     7081 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7141 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     7201 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7261 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7321 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7381 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7441 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7501 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7561 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7621 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7681 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7741 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7801 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7861 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7921 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7981 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8041 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8101 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8161 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8221 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8281 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8341 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8401 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8461 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8521 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8581 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8641 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8701 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8761 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8821 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8881 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8941 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9001 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9061 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9121 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9181 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9241 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9301 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9361 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     9421 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     9481 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     9541 tgaccgacaa ttgcatgaag aatctgctta gg
//
