LOCUS       sequence-47732-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Loque D, Lalonde S, Looger LL, von Wiren N, Frommer WB
  TITLE     A cytosolic trans-activation domain essential for ammonium uptake.
  JOURNAL   Nature. 2007 Mar 8;446(7132):195-8. Epub 2007 Feb 11.
  PUBMED    17293878
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2007-03-8"; volume: "446(7132)"; pages: "195-8. Epub 2007 Feb
            11"
COMMENT     Sequence Label: pDRf1-GW
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(153..277)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggcccc tcgaggggcc gccatagtga ctggatatgt tgtgttttac
      181 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
      241 tttacgtttc tcgttcagct tttttgtaca aacttgtgat ggccgcgaat tcactagtcg
      301 aggctggggt atattttttt tctttctttt gaatgtgtgt ataaaagaga gaaaaaatgg
      361 ttattttcca attaattaat tattaacgat tgttaaagaa aaaatttatc aacgaggttg
      421 atagaaaaaa aaagtttttg tagatataga agaaaaaaag attttcacta ttggtgttat
      481 aggaaagaaa gagaaaaaat ttaagagaaa ttagatgtta gacgataatg ataggacatt
      541 tgaaaataat acaatatttg gcctttttat ataatcgaaa ctaatggagg ggagcataag
      601 cggtacccac catcgaccac gtttcgtcac cggtcataat tggaataaac aaaacaaaca
      661 aacgaactaa agagtgtgag agtaacaatt tccacagagg taaaaaaaaa aaaaaggaaa
      721 acagaaacaa aaaaagctca tagttctact atagcttctt cagactttct gtgaacgagg
      781 gaaaaagaca acaaaaaagc aatggataaa cggtctcgat cgaggcctgg aa
//
