LOCUS       sequence-47734-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Fujita N, Hayashi-Nishino M, Fukumoto H, Omori H, Yamamoto A, Noda 
            T, Yoshimori T
  TITLE     An Atg4B mutant hampers the lipidation of LC3 paralogues and causes 
            defects in autophagosome closure.
  JOURNAL   Mol Biol Cell. 2008 Nov . 19(11):4651-9.
  PUBMED    18768752
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2008 Nov . 19(11):4651-9."
COMMENT     Sequence Label: pmStrawberry-Atg4BC74A
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..722
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(160..178)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     428..447
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcagatccgc tagcatggtg agcaagggcg aggagaataa catggccatc atcaaggagt
       61 tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      121 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      181 gtggccccct gcccttcgcc tgggacatcc taacccccaa cttcacctac ggctccaagg
      241 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      301 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      361 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
      421 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
      481 accccgagga cggcgccctg aagggcgaga tcaagatgag gctgaagctg aaggacggcg
      541 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
      601 gcgcctacat cgtcggcatc aagttggaca tcacctccca caacgaggac tacaccatcg
      661 tggaactgta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
      721 agagatccat ggacgcagcc actttgacat atgatactct ccggtttgct gaaatttgaa
      781 gatttccctg agacctcaga gcctgtttgg attctgggca gaaaatacag cattttcaca
      841 gagaaggacg aaatcttgtc
//
