LOCUS       sequence-477387-       11945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express AAV2 capsid with ncAA mutant at site 454 of VP1 and VP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Erickson SB, Pham Q, Cao X, Glicksman J, Kelemen RE, Shahraeini SS, 
            Bodkin S, Kiyam Z, Chatterjee A
  TITLE     Precise Manipulation of the Site and Stoichiometry of Capsid 
            Modification Enables Optimization of Functional Adeno-Associated 
            Virus Conjugates.
  JOURNAL   Bioconjug Chem. 2024 Jan 17;35(1):64-71. doi: 
            10.1021/acs.bioconjchem.3c00411. Epub 2023 Dec 16.
  PUBMED    38103182
REFERENCE   2  (bases 1 to 11945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.bioconjchem.3c00411"; journalName: "Bioconjug Chem"; 
            date: "2024-01-17- 17"; volume: "35"; issue: "1"; pages: "64-71"
COMMENT     Sequence Label: pIDTsmart-RC2-delVP12-VP12-454TAG
FEATURES             Location/Qualifiers
     source          1..11945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..22
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    235..282
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4231..4242
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     enhancer        4524..4903
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4904..5107
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        5152..5170
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6828..6839
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     enhancer        7118..7497
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7498..7701
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        7746..7764
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9833..9844
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(10246..10262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10246..10262)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      10310..10341
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        10342..10444
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             10445..11260
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(10518..10537)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      11284..11311
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(11323..11910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11403..11422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatccagt cgcgctgctc
      181 tcgtcgatcc gctagggcgg ccgctctaga actagtggat cccccggaag atcagaagtt
      241 cctattccga agttcctatt ctctagaaag tataggaact tctgatctgc gcagccgcca
      301 tgccggggtt ttacgagatt gtgattaagg tccccagcga ccttgacgag catctgcccg
      361 gcatttctga cagctttgtg aactgggtgg ccgagaagga atgggagttg ccgccagatt
      421 ctgacatgga tctgaatctg attgagcagg cacccctgac cgtggccgag aagctgcagc
      481 gcgactttct gacggaatgg cgccgtgtga gtaaggcccc ggaggccctt ttctttgtgc
      541 aatttgagaa gggagagagc tacttccaca tgcacgtgct cgtggaaacc accggggtga
      601 aatccatggt tttgggacgt ttcctgagtc agattcgcga aaaactgatt cagagaattt
      661 accgcgggat cgagccgact ttgccaaact ggttcgcggt cacaaagacc agaaatggcg
      721 ccggaggcgg gaacaaggtg gtggatgagt gctacatccc caattacttg ctccccaaaa
      781 cccagcctga gctccagtgg gcgtggacta atatggaaca gtatttaagc gcctgtttga
      841 atctcacgga gcgtaaacgg ttggtggcgc agcatctgac gcacgtgtcg cagacgcagg
      901 agcagaacaa agagaatcag aatcccaatt ctgatgcgcc ggtgatcaga tcaaaaactt
      961 cagccaggta catggagctg gtcgggtggc tcgtggacaa ggggattacc tcggagaagc
     1021 agtggatcca ggaggaccag gcctcataca tctccttcaa tgcggcctcc aactcgcggt
     1081 cccaaatcaa ggctgccttg gacaatgcgg gaaagattat gagcctgact aaaaccgccc
     1141 ccgactacct ggtgggccag cagcccgtgg aggacatttc cagcaatcgg atttataaaa
     1201 ttttggaact aaacgggtac gatccccaat atgcggcttc cgtctttctg ggatgggcca
     1261 cgaaaaagtt cggcaagagg aacaccatct ggctgtttgg gcctgcaact accgggaaga
     1321 ccaacatcgc ggaggccata gcccacactg tgcccttcta cgggtgcgta aactggacca
     1381 atgagaactt tcccttcaac gactgtgtcg acaagatggt gatctggtgg gaggagggga
     1441 agatgaccgc caaggtcgtg gagtcggcca aagccattct cggaggaagc aaggtgcgcg
     1501 tggaccagaa atgcaagtcc tcggcccaga tagacccgac tcccgtgatc gtcacctcca
     1561 acaccaacat gtgcgccgtg attgacggga actcaacgac cttcgaacac cagcagccgt
     1621 tgcaagaccg gatgttcaaa tttgaactca cccgccgtct ggatcatgac tttgggaagg
     1681 tcaccaagca ggaagtcaaa gactttttcc ggtgggcaaa ggatcacgtg gttgaggtgg
     1741 agcatgaatt ctacgtcaaa aagggtggag ccaagaaaag acccgccccc agtgacgcag
     1801 atataagtga gcccaaacgg gtgcgcgagt cagttgcgca gccatcgacg tcagacgcgg
     1861 aagcttcgat caactacgca gacaggtacc aaaacaaatg ttctcgtcac gtgggcatga
     1921 atctgatgct gtttccctgc agacaatgcg agagaatgaa tcagaattca aatatctgct
     1981 tcactcacgg acagaaagac tgtttagagt gctttcccgt gtcagaatct caacccgttt
     2041 ctgtcgtcaa aaaggcgtat cagaaactgt gctacattca tcatatcatg ggaaaggtgc
     2101 cagacgcttg cactgcctgc gatctggtca atgtggattt ggatgactgc atctttgaac
     2161 aataaatgat ttaaatcagg tctcgctgcc gatggttatc ttccagattg gctcgaggac
     2221 actctctctg aaggaataag acagtggtgg aagctcaaac ctggcccacc accaccaaag
     2281 cccgcagagc ggcataagga cgacagcagg ggtcttgtgc ttcctgggta caagtacctc
     2341 ggacccttca acggactcga caagggagag ccggtcaacg aggcagacgc cgcggccctc
     2401 gagcacgaca aagcctacga ccggcagctc gacagcggag acaacccgta cctcaagtac
     2461 aaccacgccg acgcggagtt tcaggagcgc cttaaagaag atacgtcttt tgggggcaac
     2521 ctcggacgag cagtcttcca ggcgaaaaag agggttcttg aacctctggg cctggttgag
     2581 gaacctgtta agaccgctcc gggaaaaaag aggccggtag agcactctcc tgtggagcca
     2641 gactcctcct cgggaaccgg aaaggcgggc cagcagcctg caagaaaaag attgaatttt
     2701 ggtcagactg gagacgcaga ctcagtacct gacccccagc ctctcggaca gccaccagca
     2761 gccccctctg gtctgggaac taatacgatg gctacaggca gtggcgcacc aatggcagac
     2821 aataacgagg gcgccgacgg agtgggtaat tcctcgggaa attggcattg cgattccaca
     2881 tggatgggcg acagagtcat caccaccagc acccgaacct gggccctgcc cacctacaac
     2941 aaccacctct acaaacaaat ttccagccaa tcaggagcct cgaacgacaa tcactacttt
     3001 ggctacagca ccccttgggg gtattttgac ttcaacagat tccactgcca cttttcacca
     3061 cgtgactggc aaagactcat caacaacaac tggggattcc gacccaagag actcaacttc
     3121 aagctcttta acattcaagt caaagaggtc acgcagaatg acggtacgac gacgattgcc
     3181 aataacctta ccagcacggt tcaggtgttt actgactcgg agtaccagct cccgtacgtc
     3241 ctcggctcgg cgcatcaagg atgcctcccg ccgttcccag cagacgtctt catggtgcca
     3301 cagtatggat acctcaccct gaacaacggg agtcaggcag taggacgctc ttcattttac
     3361 tgcctggagt actttccttc tcagatgctg cgtaccggaa acaactttac cttcagctac
     3421 acttttgagg acgttccttt ccacagcagc tacgctcaca gccagagtct ggaccgtctc
     3481 atgaatcctc tcatcgacca gtacctgtat tacttgagca gaacaaacac tccaagtgga
     3541 accaccacgc agtcaaggct tcagttttct caggccggag cgagtgacat tcgggaccag
     3601 tctaggaact ggcttcctgg accctgttac cgccagcagc gagtatcaaa gacatctgcg
     3661 gataacaaca acagtgaata ctcgtggact ggagctacca agtaccacct caatggcaga
     3721 gactctctgg tgaatccggg cccggccatg gcaagccaca aggacgatga agaaaagttt
     3781 tttcctcaga gcggggttct catctttggg aagcaaggct cagagaaaac aaatgtggac
     3841 attgaaaagg tcatgattac agacgaagag gaaatcagga caaccaatcc cgtggctacg
     3901 gagcagtatg gttctgtatc taccaacctc cagagaggca acagacaagc agctaccgca
     3961 gatgtcaaca cacaaggcgt tcttccaggc atggtctggc aggacagaga tgtgtacctt
     4021 caggggccca tctgggcaaa gattccacac acggacggac attttcaccc ctctcccctc
     4081 atgggtggat tcggacttaa acaccctcct ccacagattc tcatcaagaa caccccggta
     4141 cctgcgaatc cttcgaccac cttcagtgcg gcaaagtttg cttccttcat cacacagtac
     4201 tccacgggac aggtcagcgt ggagatcgag tgggagctgc agaaggaaaa cagcaaacgc
     4261 tggaatcccg aaattcagta cacttccaac tacaacaagt ctgttaatgt ggactttact
     4321 gtggacacta atggcgtgta ttcagagcct cgccccattg gcaccagata cctgactcgt
     4381 aatctgtaat tgcttgttaa tcaataaacc gtttaattcg tttcagttga actttggtct
     4441 ctgcgtattt ctttcttatc tagtttccat ggctacgtag ataagtagca tggcgggtta
     4501 atcattaact acacctgcag gttgacattg attattgact agttattaat agtaatcaat
     4561 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     4621 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     4681 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     4741 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     4801 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     4861 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     4921 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     4981 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     5041 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     5101 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
     5161 tcactatagg gagacccaag ctggctagca tggctccggg aaaaaagagg ccggtagagc
     5221 actctcctgt ggagccagac tcctcctcgg gaaccggaaa ggcgggccag cagcctgcaa
     5281 gaaaaagatt gaattttggt cagactggag acgcagactc agtacctgac ccccagcctc
     5341 tcggacagcc accagcagcc ccctctggtc tcggaactaa taccctcgct acaggcagtg
     5401 gcgcaccact cgcagacaat aacgagggcg ccgacggagt gggtaattcc tcgggaaatt
     5461 ggcattgcga ttccacatgg ctcggcgaca gagtcatcac caccagcacc cgaacctggg
     5521 ccctgcccac ctacaacaac cacctctaca aacaaatttc cagccaatca ggagcctcga
     5581 acgacaatca ctactttggc tacagcaccc cttgggggta ttttgacttc aacagattcc
     5641 actgccactt ttcaccacgt gactggcaaa gactcatcaa caacaactgg ggattccgac
     5701 ccaagagact caacttcaag ctctttaaca ttcaagtcaa agaggtcacg cagaatgacg
     5761 gtacgacgac gattgccaat aaccttacca gcacggttca ggtgtttact gactcggagt
     5821 accagctccc gtacgtcctc ggctcggcgc atcaaggatg cctcccgccg ttcccagcag
     5881 acgtcttcat ggtgccacag tatggatacc tcaccctgaa caacgggagt caggcagtag
     5941 gacgctcttc attttactgc ctggagtact ttccttctca gatgctgcgt accggaaaca
     6001 actttacctt cagctacact tttgaggacg ttcctttcca cagcagctac gctcacagcc
     6061 agagtctgga ccgtctcatg aatcctctca tcgaccagta cctgtattac ttgagcagaa
     6121 caaacactcc aagtggatag accacgcagt caaggcttca gttttctcag gccggagcga
     6181 gtgacattcg ggaccagtct aggaactggc ttcctggacc ctgttaccgc cagcagcgag
     6241 tatcaaagac atctgcggat aacaacaaca gtgaatactc gtggactgga gctaccaagt
     6301 accacctcaa tggcagagac tctctggtga atccgggccc ggccatggca agccacaagg
     6361 acgatgaaga aaagtttttt cctcagagcg gggttctcat ctttgggaag caaggctcag
     6421 agaaaacaaa tgtggacatt gaaaaggtca tgattacaga cgaagaggaa atcaggacaa
     6481 ccaatcccgt ggctacggag cagtatggtt ctgtatctac caacctccag agaggcaaca
     6541 gacaagcagc taccgcagat gtcaacacac aaggcgttct tccaggcatg gtctggcagg
     6601 acagagatgt gtaccttcag gggcccatct gggcaaagat tccacacacg gacggacatt
     6661 ttcacccctc tcccctcatg ggtggattcg gacttaaaca ccctcctcca cagattctca
     6721 tcaagaacac cccggtacct gcgaatcctt cgaccacctt cagtgcggca aagtttgctt
     6781 ccttcatcac acagtactcc acgggacagg tcagcgtgga gatcgagtgg gagctgcaga
     6841 aggaaaacag caaacgctgg aatcccgaaa ttcagtacac ttccaactac aacaagtctg
     6901 ttaatgtgga ctttactgtg gacactaatg gcgtgtattc agagcctcgc cccattggca
     6961 ccagatacct gactcgtaat ctgtaattgc ttgttaatca ataaaccgtt taattcgttt
     7021 cagttgaact ttggtctctg cgtatttctt tcttatctag tttccatggc tacgtagata
     7081 agtagcatgg cgggttaatc attaactaca acgcgttgac attgattatt gactagttat
     7141 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     7201 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     7261 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     7321 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     7381 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     7441 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     7501 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     7561 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     7621 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     7681 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
     7741 gaaattaata cgactcacta tagggagacc caagctggct agcatggctg ccgatggtta
     7801 tcttccagat tggctcgagg acactctctc tgaaggaata agacagtggt ggaagctcaa
     7861 acctggccca ccaccaccaa agcccgcaga gcggcataag gacgacagca ggggtcttgt
     7921 gcttcctggg tacaagtacc tcggaccctt caacggactc gacaagggag agccggtcaa
     7981 cgaggcagac gccgcggccc tcgagcacga caaagcctac gaccggcagc tcgacagcgg
     8041 agacaacccg tacctcaagt acaaccacgc cgacgcggag tttcaggagc gccttaaaga
     8101 agatacgtct tttgggggca acctcggacg agcagtcttc caggcgaaaa agagggttct
     8161 tgaacctctg ggcctggttg aggaacctgt taagaccgct ccgggaaaaa agaggccggt
     8221 agagcactct cctgtggagc cagactcctc ctcgggaacc ggaaaggcgg gccagcagcc
     8281 tgcaagaaaa agattgaatt ttggtcagac tggagacgca gactcagtac ctgaccccca
     8341 gcctctcgga cagccaccag cagccccctc tggtctcgga actaataccc tcgctacagg
     8401 cagtggcgca ccactcgcag acaataacga gggcgccgac ggagtgggta attcctcggg
     8461 aaattggcat tgcgattcca catggctcgg cgacagagtc atcaccacca gcacccgaac
     8521 ctgggccctg cccacctaca acaaccacct ctacaaacaa atttccagcc aatcaggagc
     8581 ctcgaacgac aatcactact ttggctacag caccccttgg gggtattttg acttcaacag
     8641 attccactgc cacttttcac cacgtgactg gcaaagactc atcaacaaca actggggatt
     8701 ccgacccaag agactcaact tcaagctctt taacattcaa gtcaaagagg tcacgcagaa
     8761 tgacggtacg acgacgattg ccaataacct taccagcacg gttcaggtgt ttactgactc
     8821 ggagtaccag ctcccgtacg tcctcggctc ggcgcatcaa ggatgcctcc cgccgttccc
     8881 agcagacgtc ttcatggtgc cacagtatgg atacctcacc ctgaacaacg ggagtcaggc
     8941 agtaggacgc tcttcatttt actgcctgga gtactttcct tctcagatgc tgcgtaccgg
     9001 aaacaacttt accttcagct acacttttga ggacgttcct ttccacagca gctacgctca
     9061 cagccagagt ctggaccgtc tcatgaatcc tctcatcgac cagtacctgt attacttgag
     9121 cagaacaaac actccaagtg gatagaccac gcagtcaagg cttcagtttt ctcaggccgg
     9181 agcgagtgac attcgggacc agtctaggaa ctggcttcct ggaccctgtt accgccagca
     9241 gcgagtatca aagacatctg cggataacaa caacagtgaa tactcgtgga ctggagctac
     9301 caagtaccac ctcaatggca gagactctct ggtgaatccg ggcccggcca tggcaagcca
     9361 caaggacgat gaagaaaagt tttttcctca gagcggggtt ctcatctttg ggaagcaagg
     9421 ctcagagaaa acaaatgtgg acattgaaaa ggtcatgatt acagacgaag aggaaatcag
     9481 gacaaccaat cccgtggcta cggagcagta tggttctgta tctaccaacc tccagagagg
     9541 caacagacaa gcagctaccg cagatgtcaa cacacaaggc gttcttccag gcatggtctg
     9601 gcaggacaga gatgtgtacc ttcaggggcc catctgggca aagattccac acacggacgg
     9661 acattttcac ccctctcccc tcatgggtgg attcggactt aaacaccctc ctccacagat
     9721 tctcatcaag aacaccccgg tacctgcgaa tccttcgacc accttcagtg cggcaaagtt
     9781 tgcttccttc atcacacagt actccacggg acaggtcagc gtggagatcg agtgggagct
     9841 gcagaaggaa aacagcaaac gctggaatcc cgaaattcag tacacttcca actacaacaa
     9901 gtctgttaat gtggacttta ctgtggacac taatggcgtg tattcagagc ctcgccccat
     9961 tggcaccaga tacctgactc gtaatctgta attgcttgtt aatcaataaa ccgtttaatt
    10021 cgtttcagtt gaactttggt ctctgcgtat ttctttctta tctagtttcc atggctacgt
    10081 agataagtag catggcgggt taatcattaa ctacagccct aggggtgcga gcggatcgag
    10141 cagtgtcgat cactactgga ccgcgagctg tgctgcgacc cgtgatctta cggcattata
    10201 cgtatgatcg gtccacgatc agctagatta tctagtcagc ttgatgtcat agctgtttcc
    10261 tgaggctcaa tactgaccat ttaaatcata cctgacctcc atagcagaaa gtcaaaagcc
    10321 tccgaccgga ggcttttgac ttgatcggca cgtaagaggt tccaactttc accataatga
    10381 aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc
    10441 taaaatgagc catattcaac gggaaacgtc ttgcttgaag ccgcgattaa attccaacat
    10501 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
    10561 aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
    10621 tagcgttgcc aatgatgtta cagatgagat ggtcaggcta aactggctga cggaatttat
    10681 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
    10741 tgcgatccca gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
    10801 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
    10861 tccttttaac ggcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
    10921 tttggttggt gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
    10981 gaaagaaatg cataaactct tgccattctc accggattca gtcgtcactc atggtgattt
    11041 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
    11101 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
    11161 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
    11221 taaattgcag tttcacttga tgctcgatga gtttttctaa tgaggaccta aatgtaatca
    11281 cctggctcac cttcgggtgg gcctttctgc gttgctggcg tttttccata ggctccgccc
    11341 ccctgacgag catcacaaaa atcgatgctc aagtcagagg tggcgaaacc cgacaggact
    11401 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    11461 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    11521 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    11581 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    11641 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    11701 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    11761 aagaacagta tttggtatct gcgctctgct gaagccagtt acctcggaaa aagagttggt
    11821 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    11881 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgattttcta ccgaagaaag
    11941 gccca
//
