LOCUS       sequence-477497-       10427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GAL4 under control of the rtn1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10427)
  AUTHORS   Moyer AJ, Barcus A, Capps MES, Chrabasz JA, Lalonde RL, Mosimann C, 
            Thyme SB
  TITLE     Genetic context of transgene insertion can influence 
            neurodevelopment in zebrafish.
  JOURNAL   bioRxiv [Preprint]. 2025 Mar 5:2025.02.28.640904. doi: 
            10.1101/2025.02.28.640904.
  PUBMED    40093151
REFERENCE   2  (bases 1 to 10427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Mar 5:2025.02.28.640904. doi: 
            10.1101/2025.02.28.640904."
COMMENT     Sequence Label: pTol2-rtn1a-KALTA4
FEATURES             Location/Qualifiers
     source          1..10427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1785..1803
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(3656..3667,3668..3830)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             5625..5642
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5928..6368
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    6553..6687
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(6989..7702,7703..7705,7706..7708))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6992..7702,7703..7705,7706..7708))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7034..7055)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7383..7402
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7642..7663
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(9007..9194,9195..9206)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9240..9256)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9240..9256)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    9279..9348
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(9398..9418)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9398..9416)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9437..9453)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9437..9453)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9450..9472)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9461..9477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9485..9491,9492..9509,9510..9515))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9530..9551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9668..9685)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9839..10427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9919..9938)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     1801 agggcgaatt gggtaccaaa tagtaggaat tacccacctg tacaagtgct gaaaacttgg
     1861 atgaataagc ccgtttgcca ttttctactg ctattttaaa tcttttctgt atttgtctgc
     1921 atttgtcttt acccttgaat aaatgtttta gttgtttttt ttcaattatg actgtgttta
     1981 acagacatta ttacaataat atgtaatagt agtacacact attattatgt aatagtactt
     2041 gttgactgta tttgagaact ggaccgagtg agtgttacgt cacccattca aaatgactta
     2101 cttctggctc caacaaaatg aagttaattc agttgccatt tttcactgta tggacatcgc
     2161 cgtgttggag ctagacgttg ccaataagca agaaagagcc gagatgcgtc gagtctgagt
     2221 caccgttcct atggcaaccc ctctaaccaa tcagaagtaa gcttgttgga agtccacagc
     2281 ctaccacttg aaagcgggct gcacaaaatc tgtcaaacgt tttgaacgtt ggatgtgaga
     2341 gcacatactt ttattaaggc atctggttgg tcagtttata atatcaacaa cttgggctac
     2401 agaaaagaaa agttattaca gaaattatga ttaacaagta catgttaaat aaagatttta
     2461 atatgaatgc caccactgga gcattcatgc catttggagc ttcttcctgt ttggatcact
     2521 agaaggagga ggtcactcat tacagttctc atatacagtc gttggttggt tggttggttg
     2581 gtagattgat tgattgattg attgattgat tgattgattg attgattgat tgattgattg
     2641 attgattgat tgattgattg gtagtcaaaa taagaaataa tttccacaga ttcattacag
     2701 aaatgattaa atgcatacat aaaaaactgg gggggggggg ggatacaaca acacacttaa
     2761 gtcacatttg cctacgtaaa gaaaagtaaa gaaaatcaat agctatattt tacatctcct
     2821 ttttttgctg tcttttaatt agccttgttt tgctgttatc ttgattgaaa ctgtaacttc
     2881 ttcacctgct tcttttcttt gtaggttttg ccagcccaga aacaggcatg gctgtgcaag
     2941 gccagagcac catgcacaat gcgctgcatg tcttcatgaa cggctcaatg tcctcagtcc
     3001 agggctcagc caacgacccc atcttcctcc ttcaccatgc tttcattgac aggtaacaaa
     3061 cacgtcatga cattagactg cacagttttt gacaaagttc atacaatctg ttgtttatag
     3121 ctgctacaat tagtgaagtt tgtgaatgta cttggatgag cagcgaaaga tcaattgaga
     3181 tcaattgtta gagtttggtt gccctgcaga gcaaagaaca aaaaataatc tggtggcttt
     3241 actgcgtgag gttattattg gtggaataga aacacaaaac ataattgcat ttatttgttt
     3301 aattttttat cttatcttaa ctttcatctt gcatatttgt ttcttacatc atttctagca
     3361 tctttgagcg ctggctaaga actcatcagc ctccccggtc catctaccca cgtaccaatg
     3421 caccaattgg ccacaatgac ggctactaca tggtgccatt ccttcctctt tataggaatg
     3481 gagactacct cctgtccaac aatgctcttg gatacgagta cgcctacctg ttggacccag
     3541 gtcattgcac aacaccagaa atgccctctg atctgcaaaa gacgtgaata atctgttcag
     3601 acacccatat gcactctgtt ccacacaggt cagaggtttg tccaggagtt cttgacagag
     3661 gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac aagtacttag ggaaaatttt
     3721 actcaattaa aagtaaaagt atctggctag aatcttactt gagtaaaagt aaaaaagtac
     3781 tccattaaaa ttgtacttga gtattaagga agtaaaagta aaagcaagaa agaaaactag
     3841 agattcttgt ttaagctttt aatctcaaaa aacattaaat gaaatgcata caaggtttta
     3901 tcctgcttta gaactgtttg tatttaatta tcaaactata agacagacaa tctaatgcca
     3961 gtacacgcta ctcaaagttg taaaacctca gatttaactt cagtagaagc tgattctcaa
     4021 aattgttagt gtcaagccta gctcttttgg ggctgaaaag caatcctgca gtgctgaaaa
     4081 gcctctcaca ggcagccgat gcgggaagag gtgtattagt cttgatagag aggctgcaaa
     4141 tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac acaggccaga tgggccattc
     4201 gtacgcttaa tttcgtatag ggataacagg gtaatgatct agggtactct aaaccagcga
     4261 tgcccaaaaa ctagggcccg ctggccaaag ttggcccaga ataacctttg atttggccac
     4321 cccatctgag aagagaacga gaatgatagg gaaggttgaa gcaaaccaga gattgtcaaa
     4381 acaattttac tttttgtttg tttgtttaat tgttaagcta caaaaaagca aactgaaatt
     4441 gaatgtttca gataaatgtt ctgaatgaat cagattttta atgttaaact gtaaatactg
     4501 ttacttgatg gaagacagtg ttctaaatgg gatttatata tttttattgt aaaaaaatta
     4561 tttaatttta agtatgaata aattaggaat ttaccatttg aaactttatt gtaatacact
     4621 ttagtatatt aacctttagc ccgccaacct aaatcaaatt tggtttttgg ctcttcataa
     4681 gaaaaaatgt gggcatccct gctctaaacc ttctcactca gtatgcagaa catgtgataa
     4741 gtcacataca atttgttgag catgtcttaa tatggcctgt tcataaattt gctgcagtga
     4801 catctgctct gatacaccaa taataaataa acatacattt caatggggct ggtttcatgg
     4861 tgatgacatg aaagagtcct attttgtata cttaaagaat ccttcagtaa aaattctttt
     4921 ccaataatag attttttttc acagtgcgaa gacttcataa taggacaatt ttcagcagta
     4981 aagaaccttt tgtgggataa aataattcct agttgttctt gatatatcaa ttataatgtc
     5041 ttatggaacc actgaggtca gaaagaattt tttattttat aatattgttc tccaaatatt
     5101 tcactgaccc acaacaagca cttctccagc ctctcttgga tcccagctta aaaggtgcca
     5161 aattgattta ttgtcaatta tgtttcaata tgacctttag agaatgtgtg aaggaattgt
     5221 gaagtttcca aaaagtttta ttaaattgag ttctctgggg cctttagaaa attctgatta
     5281 gtttaacagt tattaattta tgggattagt tcatgttaca ttacactaat tattcgtttt
     5341 aaacaaatca ttttgtttca attaaataat acccttttta atttcgttgt tataatgctg
     5401 attactgttt ttatgatgtt tatgatattt ataaccaagt aaaatcttgc attttcacaa
     5461 aagttcgtct gcaatgttgt taaagtcagt gtttgtgaat agaaacaaaa taaatcagac
     5521 tccacccttt ttgctccgac aacaatgcca ctgaggtcac atgaccatgc tcatgtttat
     5581 gtatgagctt tgtttggtag ggtgtggcct ctttgatctg actccatcat catcaccacc
     5641 acccccgtcg ggctctgcat atggtacggt ccgctctctg gctctgcggt ggaggctgca
     5701 gtgtttaccg tttcatccaa cagagcggct cctctcctct cctttctctt tccgtcttat
     5761 ctgccctttt cctcgttaag cagcagcatc ctctctctct ctctgctctc tcatctaatt
     5821 tctggccatt catgcggttc ggacgtgtga gatagtgggt ctctgaaaga catacgacca
     5881 gcgaaaggga aaggaaaaag acgacagcag tgagagaagc gacagacatg aaactgctct
     5941 catccatcga gcaagcctgc gacatttgtc ggcttaagaa gctgaaatgc tccaaggaaa
     6001 agccgaaatg tgccaaatgc ctgaagaaca attgggaatg tcgttactct cccaaaacca
     6061 agcgaagtcc actcacaagg gctcatctga ccgaagtgga gagcaggcta gagagactgg
     6121 aacaactctt tttgctcatc ttccctagag aggaccttga catgatcctc aagatggatt
     6181 ctctccagga tattaaagcc cttttgactg gcttattcgt ccaggacaat gtgaacaaag
     6241 acgctgtgac agaccgattg gcaagtgtcg agaccgatat gcctctgaca ctgagacagc
     6301 acagaatcag cgctacttcc tcaagcgaag agtcttctaa caagggacag agacagctga
     6361 ctgtttcgag caggtcgacc ccgtccccgg ccgacgccct ggacgacggc gacctggaca
     6421 tgctgcctgc tgatgctctc gatgatttcg atctggatat gctcccggcc gacgccctgg
     6481 acgactacga cctggacatc ctcccgggta actaacaaac tcgagaaagc tagcaaacca
     6541 tggtacgatg atgatccaga catgataaga tacattgatg agtttggaca aaccacaact
     6601 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     6661 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     6721 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     6781 gctgattatg atcctctaga tcagatctct tgtttattgc agcttataat ggttacaaat
     6841 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6901 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctacgtaata cgactcacta
     6961 tagttctaga ggctcgagag gggccgcttt acttgtacag ctcgtccatg ccgagagtga
     7021 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     7081 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     7141 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     7201 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
     7261 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     7321 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     7381 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     7441 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
     7501 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     7561 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     7621 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     7681 tgaacagctc ctcgcccttg ctcaccatgg tcactgtctg ctttgctgtt ggtctgggct
     7741 cctgggtcac tggcttacta atggagtctt tatgtatgag gactcttatc aattgttctt
     7801 ctataaaggt ctgcagtgtt tctgttcgtc ccctacatgg acacccagag cctcctaaat
     7861 acaggagccc tgataactgc acaagtgctc agattccagc agggtggaaa atgagataaa
     7921 gtgtgcagat ggggaggggg acgtgaatga gagatttgag ggatgaaaag gatggatgaa
     7981 cgcattgaaa atagcccctt tcacaaaata ataccagtaa attgtcatat aattaatgac
     8041 cagaccttta cctgtaaatg atgtgatgtt catatacaga agaatgccaa caatttacag
     8101 atgattttac aaattcttaa ttttcctttt ctaaatggat ttgttcacac attatctctt
     8161 caaagcaatt gaattttctg gaaaagattg gctgtgtgtg taaaaggtgc tactgaggaa
     8221 tttaatgtga catggaatga agcaaacagc agcctaagac aggatggcag gaaaaatgtc
     8281 acatgtttaa aataagagtg aaaccaacga cctggactta tagagactgt gtgtccttag
     8341 gcaaagctct tgtacaaaca gtgcttgtga ctgtgaaatt cggggtttgc ctggattgtg
     8401 ttaaatgtgt gcttggatgt atcataaaaa agaatcggtt ctctgtattt gacattttct
     8461 gtaatttatc taaagttaca actggctatg cctgattttt atcactattg aatcaatcat
     8521 tttaaagaat gttaagtctg ctgatttgga gagctcattt acatttattt tgaatgtctc
     8581 ttatttaaca caactgattt aagctttaga tctaatctga tcattcaggc gcgccaacac
     8641 gtgaatacca cgcgaggcct tagggataac agggtaatac gcgtgatctg cgaagatacg
     8701 gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca gctgctgatg
     8761 aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc ctaatttcca
     8821 ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga cgtcacttcc aaaggaccaa
     8881 tgaacatgtc tgaccaattt catataatgt gaaaacgatt ttcataggca gaataaataa
     8941 catttaaatt aaactgggca tcagcgcaat tcaattggtt tggtaatagc aagggaaaat
     9001 agaatgaagt gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt
     9061 aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta
     9121 attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa
     9181 aattactcaa gtactttaca cctctggttc ttgaccccct accttcagca agcccagcag
     9241 atccactagt tctagagctc gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc
     9301 cagggcgtgc ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatctga
     9361 tgaacgggcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc
     9421 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     9481 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9541 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9601 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9661 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9721 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9781 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9841 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9901 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9961 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    10021 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    10081 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10141 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    10201 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10261 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10321 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10381 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaa
//
