LOCUS       sequence-477499-       11611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GAL4 under control of a 2.8 kb elavl3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Moyer AJ, Barcus A, Capps MES, Chrabasz JA, Lalonde RL, Mosimann C, 
            Thyme SB
  TITLE     Genetic context of transgene insertion can influence 
            neurodevelopment in zebrafish.
  JOURNAL   bioRxiv [Preprint]. 2025 Mar 5:2025.02.28.640904. doi: 
            10.1101/2025.02.28.640904.
  PUBMED    40093151
REFERENCE   2  (bases 1 to 11611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Mar 5:2025.02.28.640904. doi: 
            10.1101/2025.02.28.640904."
COMMENT     Sequence Label: pTol2-elavl3-KALTA4
FEATURES             Location/Qualifiers
     source          1..11611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1785..1803
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(3656..3667,3668..3830)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             7112..7552
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    7737..7871
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(8173..8886,8887..8889,8890..8892))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8176..8886,8887..8889,8890..8892))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8218..8239)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8567..8586
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8826..8847
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(10191..10378,10379..10390)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10424..10440)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10424..10440)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    10463..10532
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(10582..10602)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10582..10600)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10621..10637)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10621..10637)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10634..10656)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10645..10661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10669..10675,10676..10693,10694..10699))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10714..10735
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10852..10869)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11023..11611)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11103..11122)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     1801 agggcgaatt gggtaccaaa tagtaggaat tacccacctg tacaagtgct gaaaacttgg
     1861 atgaataagc ccgtttgcca ttttctactg ctattttaaa tcttttctgt atttgtctgc
     1921 atttgtcttt acccttgaat aaatgtttta gttgtttttt ttcaattatg actgtgttta
     1981 acagacatta ttacaataat atgtaatagt agtacacact attattatgt aatagtactt
     2041 gttgactgta tttgagaact ggaccgagtg agtgttacgt cacccattca aaatgactta
     2101 cttctggctc caacaaaatg aagttaattc agttgccatt tttcactgta tggacatcgc
     2161 cgtgttggag ctagacgttg ccaataagca agaaagagcc gagatgcgtc gagtctgagt
     2221 caccgttcct atggcaaccc ctctaaccaa tcagaagtaa gcttgttgga agtccacagc
     2281 ctaccacttg aaagcgggct gcacaaaatc tgtcaaacgt tttgaacgtt ggatgtgaga
     2341 gcacatactt ttattaaggc atctggttgg tcagtttata atatcaacaa cttgggctac
     2401 agaaaagaaa agttattaca gaaattatga ttaacaagta catgttaaat aaagatttta
     2461 atatgaatgc caccactgga gcattcatgc catttggagc ttcttcctgt ttggatcact
     2521 agaaggagga ggtcactcat tacagttctc atatacagtc gttggttggt tggttggttg
     2581 gtagattgat tgattgattg attgattgat tgattgattg attgattgat tgattgattg
     2641 attgattgat tgattgattg gtagtcaaaa taagaaataa tttccacaga ttcattacag
     2701 aaatgattaa atgcatacat aaaaaactgg gggggggggg ggatacaaca acacacttaa
     2761 gtcacatttg cctacgtaaa gaaaagtaaa gaaaatcaat agctatattt tacatctcct
     2821 ttttttgctg tcttttaatt agccttgttt tgctgttatc ttgattgaaa ctgtaacttc
     2881 ttcacctgct tcttttcttt gtaggttttg ccagcccaga aacaggcatg gctgtgcaag
     2941 gccagagcac catgcacaat gcgctgcatg tcttcatgaa cggctcaatg tcctcagtcc
     3001 agggctcagc caacgacccc atcttcctcc ttcaccatgc tttcattgac aggtaacaaa
     3061 cacgtcatga cattagactg cacagttttt gacaaagttc atacaatctg ttgtttatag
     3121 ctgctacaat tagtgaagtt tgtgaatgta cttggatgag cagcgaaaga tcaattgaga
     3181 tcaattgtta gagtttggtt gccctgcaga gcaaagaaca aaaaataatc tggtggcttt
     3241 actgcgtgag gttattattg gtggaataga aacacaaaac ataattgcat ttatttgttt
     3301 aattttttat cttatcttaa ctttcatctt gcatatttgt ttcttacatc atttctagca
     3361 tctttgagcg ctggctaaga actcatcagc ctccccggtc catctaccca cgtaccaatg
     3421 caccaattgg ccacaatgac ggctactaca tggtgccatt ccttcctctt tataggaatg
     3481 gagactacct cctgtccaac aatgctcttg gatacgagta cgcctacctg ttggacccag
     3541 gtcattgcac aacaccagaa atgccctctg atctgcaaaa gacgtgaata atctgttcag
     3601 acacccatat gcactctgtt ccacacaggt cagaggtttg tccaggagtt cttgacagag
     3661 gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac aagtacttag ggaaaatttt
     3721 actcaattaa aagtaaaagt atctggctag aatcttactt gagtaaaagt aaaaaagtac
     3781 tccattaaaa ttgtacttga gtattaagga agtaaaagta aaagcaagaa agaaaactag
     3841 agattcttgt ttaagctttt aatctcaaaa aacattaaat gaaatgcata caaggtttta
     3901 tcctgcttta gaactgtttg tatttaatta tcaaactata agacagacaa tctaatgcca
     3961 gtacacgcta ctcaaagttg taaaacctca gatttaactt cagtagaagc tgattctcaa
     4021 aattgttagt gtcaagccta gctcttttgg ggctgaaaag caatcctgca gtgctgaaaa
     4081 gcctctcaca ggcagccgat gcgggaagag gtgtattagt cttgatagag aggctgcaaa
     4141 tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac acaggccaga tgggccattc
     4201 gtacgcttaa tttcgtatag ggataacagg gtaatgatct aggttacaag ctttatccaa
     4261 attaagccat tttaaaacac tgccaattga aaatacaaat cctggaaaaa atcgtcttta
     4321 gcgcagtcat ttgagccatc ctaatccgtt acctcagacc ataataagaa gggataacac
     4381 tagctgtagc aatggaacac atctgtttca cacaatcata tctcctgcgc cggtgctaag
     4441 cagattcagc gtgatcataa catgctttcc actcataaat gtaaatttac aatttgcaca
     4501 tgtaaaacag acacttttga gatattggat aaaaaaacaa gagtatattg cttagtttca
     4561 tccaccagtc atccccacag cgtttggaag gccataaaaa gtgtctaaaa tcaatgatca
     4621 ttgaaagagc acaagagaga ctcttacgct gtaatgccac tggggacaaa agtgacagtc
     4681 tcttaatggg ctcttctgga ggggctcctg aacattaaaa attatcagcg aaattaccga
     4741 aagagcttca agcaactggc atgcttgatc ctctgcgtcg gggcggtgaa taggtgcttc
     4801 agatgccctc ttacccacgg gctggattca gctgccccgc taccagcgga gaccccctaa
     4861 tgagcctctg caattaagtt tattcatgtt aagtgtgaac ggggtgcgtg cggaactgtg
     4921 ggcagctaac agacctgggt tctttgtgcc acaagtgctg cctttattcg gctcacaaag
     4981 cagaaaacaa cacccgcacc tataatggcg ccctcggctg ggtctaagaa acgtggcgag
     5041 ttgacagagc agagtgggcg gggttaagac agactgacag cgggacccat ctccatcctc
     5101 ttattaacgc ttaacgagtg ccttcctcat gcaatattca tcgccactaa tatcatccaa
     5161 gctctgagct gagctggcca cttatgtaag gcaattatgt aaaatatcag acagggccca
     5221 cactcagaat ctgactgggg tagagacgcg ggacgagaac cgagagcaag aactgaaagt
     5281 gaaagtgacc actaaaggga ggagaggaca gaggggcagg atgtgtcaag attaccagag
     5341 aacacttggc cagaaatgcg caaccattgg agctctccgg attacccaaa ggttaacgag
     5401 tttgaacgcc tctgcccact cgcccatctc tgatggtttc ccaagaactc ctcaagcaaa
     5461 atatatataa ttgtgtgtat tatgcacaga cacgagaaaa tgctgttttt ctgatctgca
     5521 ttacagcaca tttgcccgcc aacgacaata ccacccactc ggtacctcgc tgactcctga
     5581 tgcctgatac ctgcgcggtg actgtctaca atctgcataa tcaagagaag ttgtgttgaa
     5641 gacgagcgcc acacaaccgt ttccacaagg tcacccaagg ccggtgcaga tgtaggtgag
     5701 gtctccataa acagactgaa ataaacacat cctccgctgg gaacaacaac cccctcacgc
     5761 ctcatgcatt tccataagcc tacatgcatc tcttccaact tatggagact cgcacctacc
     5821 aacatccgca caacaaagat atacagagcg cgctccctca ggtcaaggcc ctgtgggggt
     5881 ctgtgcagaa ataggtcatt tgtcacacat caagtcctgg ggcaggagat gcattataga
     5941 tgagaccaaa cagcctgtct cggtgagctc tacccactcc ctgagactag aaatggggga
     6001 agggagcttg agataacaac cgctgcaatc actgtgtcga tgtttaatat cagcaccaac
     6061 cgggaacaat aaggagatgg gtgcattcat gttcacatct taccagtcaa gtatcatcga
     6121 accggcttga taaccacacc tcgtgtaata gctgagcaga tagttgtcat tttaaagcgt
     6181 tggcctttgt cgattatgta atgcgcacat tcaacacatg gtaatataga aacggttatg
     6241 tcgaggttgt tttgtccaga gatgaccttc acacagttac agccgctctg catccacaca
     6301 aatggaggac ttaatcgtgg actgcattct tagaaatgat ctacaaagac aaataatgtg
     6361 aaatcaagaa aggacaaaat ttaagtaagg ggatgaggga gagagagaac gaggggcaag
     6421 gagaaagcat ggctcctgtc tttttctgca cccatctgtt cggagtgcag gtggagctct
     6481 attcactcag ctctgcatgt gtgtttgggg ggggcaggaa gaaagggagg gcaaaaggaa
     6541 gagtggagag atggtggggg ctggagggat ggggggttct cggtgatctc tcctgaaggg
     6601 gataatggga gagcagcgct ttgcaatggc tgccatgtag taccctccct gcacaattag
     6661 ccaatcagca gcaagctctg ccagccagaa ggacacataa aagaagaaca ttgcagcaga
     6721 ggcacagaag gagcctgcga ggagctggga aatacacaca caacagcaga accacaacac
     6781 cctcccctgg acacacccta ctggggatca ctgcttttct ttttttctga accatcgccc
     6841 acgccacacg gagagaaatc tctctctcat catcatcctg aagaaaaccc ccttatcctc
     6901 attttcacac tgctgaggaa aacctacaat cgcacgggct gagatttcct ggcgaagact
     6961 gtcctttttc ctttttcttt ttttttcttt tcctttggaa actgacattt gcatttctcc
     7021 attccaagcc acggcgtaat aatatctgca atccagcctg aagacctgca aatcgaagga
     7081 cctagatcac tatcatcttt gtacgtcaag aatgaaactg ctctcatcca tcgagcaagc
     7141 ctgcgacatt tgtcggctta agaagctgaa atgctccaag gaaaagccga aatgtgccaa
     7201 atgcctgaag aacaattggg aatgtcgtta ctctcccaaa accaagcgaa gtccactcac
     7261 aagggctcat ctgaccgaag tggagagcag gctagagaga ctggaacaac tctttttgct
     7321 catcttccct agagaggacc ttgacatgat cctcaagatg gattctctcc aggatattaa
     7381 agcccttttg actggcttat tcgtccagga caatgtgaac aaagacgctg tgacagaccg
     7441 attggcaagt gtcgagaccg atatgcctct gacactgaga cagcacagaa tcagcgctac
     7501 ttcctcaagc gaagagtctt ctaacaaggg acagagacag ctgactgttt cgagcaggtc
     7561 gaccccgtcc ccggccgacg ccctggacga cggcgacctg gacatgctgc ctgctgatgc
     7621 tctcgatgat ttcgatctgg atatgctccc ggccgacgcc ctggacgact acgacctgga
     7681 catcctcccg ggtaactaac aaactcgaga aagctagcaa accatggtac gatgatgatc
     7741 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     7801 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     7861 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     7921 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc
     7981 tagatcagat ctcttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8041 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8101 caatgtatct tatcatgtct ggatctacgt aatacgactc actatagttc tagaggctcg
     8161 agaggggccg ctttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
     8221 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
     8281 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
     8341 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
     8401 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
     8461 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
     8521 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
     8581 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
     8641 gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag
     8701 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
     8761 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
     8821 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
     8881 cttgctcacc atggtcactg tctgctttgc tgttggtctg ggctcctggg tcactggctt
     8941 actaatggag tctttatgta tgaggactct tatcaattgt tcttctataa aggtctgcag
     9001 tgtttctgtt cgtcccctac atggacaccc agagcctcct aaatacagga gccctgataa
     9061 ctgcacaagt gctcagattc cagcagggtg gaaaatgaga taaagtgtgc agatggggag
     9121 ggggacgtga atgagagatt tgagggatga aaaggatgga tgaacgcatt gaaaatagcc
     9181 cctttcacaa aataatacca gtaaattgtc atataattaa tgaccagacc tttacctgta
     9241 aatgatgtga tgttcatata cagaagaatg ccaacaattt acagatgatt ttacaaattc
     9301 ttaattttcc ttttctaaat ggatttgttc acacattatc tcttcaaagc aattgaattt
     9361 tctggaaaag attggctgtg tgtgtaaaag gtgctactga ggaatttaat gtgacatgga
     9421 atgaagcaaa cagcagccta agacaggatg gcaggaaaaa tgtcacatgt ttaaaataag
     9481 agtgaaacca acgacctgga cttatagaga ctgtgtgtcc ttaggcaaag ctcttgtaca
     9541 aacagtgctt gtgactgtga aattcggggt ttgcctggat tgtgttaaat gtgtgcttgg
     9601 atgtatcata aaaaagaatc ggttctctgt atttgacatt ttctgtaatt tatctaaagt
     9661 tacaactggc tatgcctgat ttttatcact attgaatcaa tcattttaaa gaatgttaag
     9721 tctgctgatt tggagagctc atttacattt attttgaatg tctcttattt aacacaactg
     9781 atttaagctt tagatctaat ctgatcattc aggcgcgcca acacgtgaat accacgcgag
     9841 gccttaggga taacagggta atacgcgtga tctgcgaaga tacggccacg ggtgctcttg
     9901 atcctgtggc tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc
     9961 cattttcttc cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc
    10021 attgtggtaa tagatgtgac atgacgtcac ttccaaagga ccaatgaaca tgtctgacca
    10081 atttcatata atgtgaaaac gattttcata ggcagaataa ataacattta aattaaactg
    10141 ggcatcagcg caattcaatt ggtttggtaa tagcaaggga aaatagaatg aagtgatctc
    10201 caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt
    10261 tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa
    10321 gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt
    10381 tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac tagttctaga
    10441 gctcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg
    10501 gctccccggg cgcgtactcc acctcaccca tctggtccat ctgatgaacg ggccaccgcg
    10561 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
    10621 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    10681 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    10741 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    10801 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    10861 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    10921 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    10981 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    11041 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    11101 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    11161 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    11221 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    11281 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    11341 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    11401 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    11461 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    11521 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    11581 gcagattacg cgcagaaaaa aaggatctca a
//
