LOCUS       sequence-477569-       11603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11603)
  AUTHORS   Kumar P, Lyle KS, Gierke S, Matov A, Danuser G, Wittmann T
  TITLE     GSK3beta phosphorylation modulates CLASP-microtubule association and
            lamella microtubule attachment.
  JOURNAL   J Cell Biol. 2009 Mar 23. 184(6):895-908.
  PUBMED    19289791
REFERENCE   2  (bases 1 to 11603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2009 Mar 23. 184(6):895-908."
COMMENT     Sequence Label: pShuttle-mRFP-gsk3 s9a
FEATURES             Location/Qualifiers
     source          1..11603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        399..702
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        703..906
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     857..877
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             952..969
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             979..1008
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1027..1701
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKEPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             2537..2548
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             3054..3071
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3081..3110
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3129..3803
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKEPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             4639..4650
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    5250..5371
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5287..5306)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5341..5360
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8562..8664
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(8753..8770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8924..9512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9004..9023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10344..11138
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10344..11138
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10398..10417)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11008..11027
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(11186..11566)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11248..11269)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11460..11479
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt
      361 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      421 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      481 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      541 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      601 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      661 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      721 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      781 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      841 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      901 agagctggtt tagtgaaccg tcagatccgc tagagatctg gtaccatggc acaccatcac
      961 caccaccacg ggagcgggga gcagaaacta atatcagagg aggatcttgg ctcaggttca
     1021 ggatctatgg cctcctccga ggacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg
     1081 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     1141 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
     1201 gacatcctgt cccctcagtt ccagtacggc tccaaggcct acgtgaagca ccccgccgac
     1261 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     1321 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     1381 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     1441 aagaccatgg gctgggaggc ctccaccgag cggatgtacc ccgaggacgg cgccctgaag
     1501 ggcgagatca agatgaggct gaagctgaag gacggcggcc actacgacgc cgaggtcaag
     1561 accacctaca tggccaagga gcccgtgcag ctgcccggcg cctacaagac cgacatcaag
     1621 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgcgccgag
     1681 ggccgccact ccaccggcgc cggttctgga tccgaattca tgtcagggcg gcccagaacc
     1741 accgcctttg cggagagctg caagccggtg cagcagcctt cagcttttgg cagcatgaaa
     1801 gttagcagag acaaggacgg cagcaaggtg acaacagtgg tggcaactcc tgggcagggt
     1861 ccagacaggc cacaagaagt cagctataca gacactaaag tgattggaaa tggatcattt
     1921 ggtgtggtat atcaagccaa actttgtgat tcaggagaac tggtcgccat caagaaagta
     1981 ttgcaggaca agagatttaa gaatcgagag ctccagatca tgagaaagct agatcactgt
     2041 aacatagtcc gattgcgtta tttcttctac tccagtggtg agaagaaaga tgaggtctat
     2101 cttaatctgg tgctggacta tgttccggaa acagtataca gagttgccag acactatagt
     2161 cgagccaaac agacgctccc tgtgatttat gtcaagttgt atatgtatca gctgttccga
     2221 agtttagcct atatccattc ctttggaatc tgccatcggg atattaaacc gcagaacctc
     2281 ttgttggatc ctgatactgc tgtattaaaa ctctgtgact ttggaagtgc aaagcagctg
     2341 gtccgaggag aacccaatgt ttcgtatatc tgttctcggt actatagggc accagagttg
     2401 atctttggag ccactgatta tacctctagt atagatgtat ggtctgctgg ctgtgtgttg
     2461 gctgagctgt tactaggaca accaatattt ccaggggata gtggtgtgga tcagttggta
     2521 gaaataatca aggtcctggg aactccaaca agggagcaaa tcagagaaat gaacccaaac
     2581 tacacagaat ttaaattccc tcaaattaag gcacatcctt ggactaaggt cttccgaccc
     2641 cgaactccac cggaggcaat tgcactgtgt agccgtctgc tggagtatac accaactgcc
     2701 cgactaacac cactggaagc ttgtgcacat tcattttttg atgaattacg ggacccaaat
     2761 gtcaaactac caaatgggcg agacacacct gcactcttca acttcaccac tcaagaactg
     2821 tcaagtaatc cacctctggc taccatcctt attcctcctc atgctcggat tcaagcagct
     2881 gcttcaaccc ccacaaatgc cacagcagcg tcagatgcta atactggaga ccgtggacag
     2941 accaataatg ctgcttctgc atcagcttcc aactccacct gagcggccgc agaagggcga
     3001 attccagcac actggcggcc gttactagtg gatccgagct cggtaccatg gcacaccatc
     3061 accaccacca cgggagcggg gagcagaaac taatatcaga ggaggatctt ggctcaggtt
     3121 caggatctat ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca
     3181 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     3241 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     3301 gggacatcct gtcccctcag ttccagtacg gctccaaggc ctacgtgaag caccccgccg
     3361 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     3421 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     3481 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     3541 agaagaccat gggctgggag gcctccaccg agcggatgta ccccgaggac ggcgccctga
     3601 agggcgagat caagatgagg ctgaagctga aggacggcgg ccactacgac gccgaggtca
     3661 agaccaccta catggccaag gagcccgtgc agctgcccgg cgcctacaag accgacatca
     3721 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgcgccg
     3781 agggccgcca ctccaccggc gccggttctg gatccgaatt catgtcaggg cggcccagaa
     3841 ccaccgcctt tgcggagagc tgcaagccgg tgcagcagcc ttcagctttt ggcagcatga
     3901 aagttagcag agacaaggac ggcagcaagg tgacaacagt ggtggcaact cctgggcagg
     3961 gtccagacag gccacaagaa gtcagctata cagacactaa agtgattgga aatggatcat
     4021 ttggtgtggt atatcaagcc aaactttgtg attcaggaga actggtcgcc atcaagaaag
     4081 tattgcagga caagagattt aagaatcgag agctccagat catgagaaag ctagatcact
     4141 gtaacatagt ccgattgcgt tatttcttct actccagtgg tgagaagaaa gatgaggtct
     4201 atcttaatct ggtgctggac tatgttccgg aaacagtata cagagttgcc agacactata
     4261 gtcgagccaa acagacgctc cctgtgattt atgtcaagtt gtatatgtat cagctgttcc
     4321 gaagtttagc ctatatccat tcctttggaa tctgccatcg ggatattaaa ccgcagaacc
     4381 tcttgttgga tcctgatact gctgtattaa aactctgtga ctttggaagt gcaaagcagc
     4441 tggtccgagg agaacccaat gtttcgtata tctgttctcg gtactatagg gcaccagagt
     4501 tgatctttgg agccactgat tatacctcta gtatagatgt atggtctgct ggctgtgtgt
     4561 tggctgagct gttactagga caaccaatat ttccagggga tagtggtgtg gatcagttgg
     4621 tagaaataat caaggtcctg ggaactccaa caagggagca aatcagagaa atgaacccaa
     4681 actacacaga atttaaattc cctcaaatta aggcacatcc ttggactaag gtcttccgac
     4741 cccgaactcc accggaggca attgcactgt gtagccgtct gctggagtat acaccaactg
     4801 cccgactaac accactggaa gcttgtgcac attcattttt tgatgaatta cgggacccaa
     4861 atgtcaaact accaaatggg cgagacacac ctgcactctt caacttcacc actcaagaac
     4921 tgtcaagtaa tccacctctg gctaccatcc ttattcctcc tcatgctcgg attcaagcag
     4981 ctgcttcaac ccccacaaat gccacagcag cgtcagatgc taatactgga gaccgtggac
     5041 agaccaataa tgctgcttct gcatcagctt ccaactccac ctgagcggcc gctcgagcct
     5101 aagcttctag ataagatatc cgatccaccg gatctagata actgatcata atcagccata
     5161 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     5221 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     5281 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5341 gtggtttgtc caaactcatc aatgtatctt aacgcggatc tgggcgtggt taagggtggg
     5401 aaagaatata taaggtgggg gtcttatgta gttttgtatc tgttttgcag cagccgccgc
     5461 cgccatgagc accaactcgt ttgatggaag cattgtgagc tcatatttga caacgcgcat
     5521 gcccccatgg gccggggtgc gtcagaatgt gatgggctcc agcattgatg gtcgccccgt
     5581 cctgcccgca aactctacta ccttgaccta cgagaccgtg tctggaacgc cgttggagac
     5641 tgcagcctcc gccgccgctt cagccgctgc agccaccgcc cgcgggattg tgactgactt
     5701 tgctttcctg agcccgcttg caagcagtgc agcttcccgt tcatccgccc gcgatgacaa
     5761 gttgacggct cttttggcac aattggattc tttgacccgg gaacttaatg tcgtttctca
     5821 gcagctgttg gatctgcgcc agcaggtttc tgccctgaag gcttcctccc ctcccaatgc
     5881 ggtttaaaac ataaataaaa aaccagactc tgtttggatt tggatcaagc aagtgtcttg
     5941 ctgtctttat ttaggggttt tgcgcgcgcg gtaggcccgg gaccagcggt ctcggtcgtt
     6001 gagggtcctg tgtatttttt ccaggacgtg gtaaaggtga ctctggatgt tcagatacat
     6061 gggcataagc ccgtctctgg ggtggaggta gcaccactgc agagcttcat gctgcggggt
     6121 ggtgttgtag atgatccagt cgtagcagga gcgctgggcg tggtgcctaa aaatgtcttt
     6181 cagtagcaag ctgattgcca ggggcaggcc cttggtgtaa gtgtttacaa agcggttaag
     6241 ctgggatggg tgcatacgtg gggatatgag atgcatcttg gactgtattt ttaggttggc
     6301 tatgttccca gccatatccc tccggggatt catgttgtgc agaaccacca gcacagtgta
     6361 tccggtgcac ttgggaaatt tgtcatgtag cttagaagga aatgcgtgga agaacttgga
     6421 gacgcccttg tgacctccaa gattttccat gcattcgtcc ataatgatgg caatgggccc
     6481 acgggcggcg gcctgggcga agatatttct gggatcacta acgtcatagt tgtgttccag
     6541 gatgagatcg tcataggcca tttttacaaa gcgcgggcgg agggtgccag actgcggtat
     6601 aatggttcca tccggcccag gggcgtagtt accctcacag atttgcattt cccacgcttt
     6661 gagttcagat ggggggatca tgtctacctg cggggcgatg aagaaaacgg tttccggggt
     6721 aggggagatc agctgggaag aaagcaggtt cctgagcagc tgcgacttac cgcagccggt
     6781 gggcccgtaa atcacaccta ttaccggctg caactggtag ttaagagagc tgcagctgcc
     6841 gtcatccctg agcagggggg ccacttcgtt aagcatgtcc ctgactcgca tgttttccct
     6901 gaccaaatcc gccagaaggc gctcgccgcc cagcgatagc agttcttgca aggaagcaaa
     6961 gtttttcaac ggtttgagac cgtccgccgt aggcatgctt ttgagcgttt gaccaagcag
     7021 ttccaggcgg tcccacagct cggtcacctg ctctacggca tctcgatcca gcatatctcc
     7081 tcgtttcgcg ggttggggcg gctttcgctg tacggcagta gtcggtgctc gtccagacgg
     7141 gccagggtca tgtctttcca cgggcgcagg gtcctcgtca gcgtagtctg ggtcacggtg
     7201 aaggggtgcg ctccgggctg cgcgctggcc agggtgcgct tgaggctggt cctgctggtg
     7261 ctgaagcgct gccggtcttc gccctgcgcg tcggccaggt agcatttgac catggtgtca
     7321 tagtccagcc cctccgcggc gtggcccttg gcgcgcagct tgcccttgga ggaggcgccg
     7381 cacgaggggc agtgcagact tttgagggcg tagagcttgg gcgcgagaaa taccgattcc
     7441 ggggagtagg catccgcgcc gcaggccccg cagacggtct cgcattccac gagccaggtg
     7501 agctctggcc gttcggggtc aaaaaccagg tttcccccat gctttttgat gcgtttctta
     7561 cctctggttt ccatgagccg gtgtccacgc tcggtgacga aaaggctgtc cgtgtccccg
     7621 tatacagact tgagagggag tttaaacgaa ttcaatagct tgttgcatgg gcggcgatat
     7681 aaaatgcaag gtgctgctca aaaaatcagg caaagcctcg cgcaaaaaag aaagcacatc
     7741 gtagtcatgc tcatgcagat aaaggcaggt aagctccgga accaccacag aaaaagacac
     7801 catttttctc tcaaacatgt ctgcgggttt ctgcataaac acaaaataaa ataacaaaaa
     7861 aacatttaaa cattagaagc ctgtcttaca acaggaaaaa caacccttat aagcataaga
     7921 cggactacgg ccatgccggc gtgaccgtaa aaaaactggt caccgtgatt aaaaagcacc
     7981 accgacagct cctcggtcat gtccggagtc ataatgtaag actcggtaaa cacatcaggt
     8041 tgattcacat cggtcagtgc taaaaagcga ccgaaatagc ccgggggaat acatacccgc
     8101 aggcgtagag acaacattac agcccccata ggaggtataa caaaattaat aggagagaaa
     8161 aacacataaa cacctgaaaa accctcctgc ctaggcaaaa tagcaccctc ccgctccaga
     8221 acaacataca gcgcttccac agcggcagcc ataacagtca gccttaccag taaaaaagaa
     8281 aacctattaa aaaaacacca ctcgacacgg caccagctca atcagtcaca gtgtaaaaaa
     8341 gggccaagtg cagagcgagt atatatagga ctaaaaaatg acgtaacggt taaagtccac
     8401 aaaaaacacc cagaaaaccg cacgcgaacc tacgcccaga aacgaaagcc aaaaaaccca
     8461 caacttcctc aaatcgtcac ttccgttttc ccacgttacg tcacttccca ttttaagaaa
     8521 actacaattc ccaacacata caagttactc cgccctaaaa cctacgtcac ccgccccgtt
     8581 cccacgcccc gcgccacgtc acaaactcca ccccctcatt atcatattgg cttcaatcca
     8641 aaataaggta tattattgat gatgttaatt aacatgcatg gatccatatg cggtgtgaaa
     8701 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     8761 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8821 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8881 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8941 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9001 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9061 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9121 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9181 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9241 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9301 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9361 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9421 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9481 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9541 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9601 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9661 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9721 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9781 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9841 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9901 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9961 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc agccatgaga ttatcaaaaa
    10021 ggatcttcac ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc
    10081 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc
    10141 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa
    10201 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa
    10261 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag
    10321 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
    10381 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
    10441 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
    10501 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
    10561 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
    10621 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
    10681 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
    10741 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
    10801 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
    10861 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
    10921 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
    10981 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
    11041 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
    11101 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa tttttgttaa
    11161 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
    11221 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
    11281 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
    11341 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
    11401 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
    11461 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
    11521 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca
    11581 ggatcgaatt aattcttaat taa
//
