LOCUS       sequence-477582-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses alpha synuclein (missing exon 3 and 5, residues 41-54 and 
            103-130) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   SanGiovanni DQ, McGlinchey RP, Lee JC
  TITLE     Amyloid formation of alternatively spliced variants of 
            alpha-synuclein.
  JOURNAL   Protein Sci. 2025 Jul;34(7):e70195. doi: 10.1002/pro.70195.
  PUBMED    40522183
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2025-07"; volume: "34(7):e70195. doi"; pages: " 
            10.1002/pro.70195"
COMMENT     Sequence Label: pET21a asyn(Delta-41-54/Delta-103-130)
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             29..51
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             362..379
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(432..450)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      446..493
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      530..888
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(617..636)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     827..848
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gnnnnanntn tccccctctn naaataattt tgtttaactt taagaaggag atatacatat
       61 ggatgtattc atgaaaggac tttcaaaggc caaggaggga gttgtggctg ctgctgagaa
      121 aaccaaacag ggtgtggcag aagcagcagg aaagacaaaa gagggtgttc tctatgtagt
      181 ggctgagaag accaaagagc aagtgacaaa tgttggagga gcagtggtga cgggtgtgac
      241 agcagtagcc cagaagacag tggagggagc agggagcatt gcagcagcca ctggctttgt
      301 caaaaaggac cagttgggca aggaagggta tcaagactat gaacctgaag cctaactcga
      361 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      421 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      481 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
      541 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      601 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      661 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
      721 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
      781 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
      841 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggat
//
