LOCUS       sequence-477595-       14234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to express the LUC gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14234)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 14234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVT-LUC
FEATURES             Location/Qualifiers
     source          1..14234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    938..1111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1153..2805)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     2721..2739
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        complement(2874..3550)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(2917..2937)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(7320..7337)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7320..7336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7329..7351)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7539..7563
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8864..9493
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9922..10995
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11061..11255
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11491..11513)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11599..11739
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11654..11673
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11754..11771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11925..12513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12005..12024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12600..13394)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13819..13843
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    14111..14132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14147..14152,14153..14170,14171..14177)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14185..14201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14190..14212
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14209..14225
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14209..14225
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtgat ctggatttta gtatggattt
      961 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     1021 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     1081 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
     1141 cggcatctac tcttacacgg cgatctttcc gcccttcttg gcctttatga ggatctctct
     1201 gatttttctt gcgtcgagtt ttccggtaag acctttcggt acttcgtcca caaacacaac
     1261 tcctccgcgc aactttttcg cggttgttac ttgactggcg acgtaatcca cgatctcttt
     1321 ttccgtcatc gtctttccgt gctccaaaac aacaacggcg gcgggaagtt caccggcgtc
     1381 atcgtcggga agacctgcga cacctgcgtc gaagatgttg gggtgttgga gcaagatgga
     1441 ttccaattca gcgggagcca cctgatagcc tttgtactta atcagagact tcaggcggtc
     1501 aacgatgaag aagtgttcgt cttcgtccca gtaagctatg tctccagaat gtagccatcc
     1561 atccttgtca atcaaggcgt tggtcgcttc cggattgttt acataaccgg acataatcat
     1621 aggacctctc acacacagtt cgcctctttg attaacgccc agcgttttcc cggtatccag
     1681 atccacaacc ttcgcttcaa aaaatggaac aactttaccg accgcgcccg gtttatcatc
     1741 cccctcgggt gtaatcagaa tagctgatgt agtctcagtg agcccatatc cttgcctgat
     1801 acctggcaga tggaacctct tggcaaccgc ttccccgact tccttagaga ggggagcgcc
     1861 accagaagca atttcgtgta aattagataa atcgtatttg tcaatcagag tgcttttggc
     1921 gaagaaggag aatagggttg gcaccagcag cgcactttga atcttgtaat cctgaaggct
     1981 cctcagaaac agctcttctt caaatctata cattaagacg actcgaaatc cacatatcaa
     2041 atatccgagt gtagtaaaca ttccaaaacc gtgatggaat ggaacaacac ttaaaatcgc
     2101 agtatccgga atgatttgat tgccaaaaat aggatctctg gcatgcgaga atctcacgca
     2161 ggcagttcta tgaggcagag cgacaccttt aggcagacca gtagatccag aggagttcat
     2221 gatcagtgca attgtcttgt ccctatcgaa ggactctggc acaaaatcgt attcattaaa
     2281 accgggaggt agatgagatg tgacgaacgt gtacatcgac tgaaatccct ggtaatccgt
     2341 tttagaatcc atgataataa ttttttggat gattgggagc tttttttgca cgttcaaaat
     2401 tttttgcaac ccctttttgg aaacgaacac cacggtaggc tgcgaaatgc ccatactgtt
     2461 gagcaattca cgttcattat aaatgtcgtt cgcgggcgca actgcaactc cgataaataa
     2521 cgcgcccaac accggcataa agaattgaag agagttttca ctgcatacga cgattctgtg
     2581 atttgtattc agcccatatc gtttcatagc ttctgccaac cgaacggaca tttcgaagta
     2641 ctcagcgtaa gtgatgtcca cctcgatatg tgcatctgta aaagcaattg ttccaggaac
     2701 cagggcgtat ctcttcatag ccttatgcag ttgctctcca gcggttccat cttccagcgg
     2761 atagaatggc gccgggcctt tctttatgtt tttggcgtct tccatggatc tcattgcccc
     2821 ccggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt
     2881 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
     2941 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
     3001 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
     3061 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
     3121 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
     3181 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
     3241 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttc
     3301 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
     3361 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
     3421 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
     3481 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
     3541 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
     3601 gagagtgtcg tgctccacca tgttggcaag ctgctctagc tcacgttgac ggaaactgca
     3661 gaacgatctc agttaggtca tgggaaagct gatattcatc cctctgagac tctatataat
     3721 taaatgcgtt tgggggatta gctaattgag aatccatata gagaaatatg gccgcgcagc
     3781 gacgccggtt gaagaatatg aacaaggaat aagatgaaca aggaagaaga tgaacaagca
     3841 ataatttaat taattattgt taataagcat aaattatgaa aataattttg atggtaatga
     3901 aagtattata aataatattt aggagaaaat attgttaagg aaatatctgg ttatgtttat
     3961 ttgttaaggt atatgggttt ttatagaaac ccaggagcag ctatggtaag aaacccagga
     4021 ctcaaaaaat gcctctcaac ttctctcata tgttgcggag tctggagtcc catttatacc
     4081 atagaaagca ttgggggtat agcatggtaa tttcacatcg gactccatac cgcggccatc
     4141 tatataatat tactgatggc cgcgcgaagt cccggtatgc cttttcagcc gttggatctg
     4201 gagccgtacc tttttcgcgt tcccattttg cccctggagt cccgagctat ttacgacccc
     4261 ctgccaccga gtccccttcg tggagcgctc ttcgtccaac acgtgtccca acttttgcgc
     4321 atttttgact acctgtcgcg cacttgaatt aaagttgccc ttttattttc ttccaattat
     4381 tttgcggctg agtagtctag tggatccccc ggagctccac ttgggatagg ttaagaaaat
     4441 atgtctggct tgtaaacgaa aagaatcggg gtttctaggc attttttaaa tagtgaccca
     4501 ggactcaaaa actagctctc aacttctctc atatgttgtg gagtctggag tcctttatat
     4561 atgtttacgc tatagaaggc atttggggac tctcccgggc ccaccatagg actccatacc
     4621 gcggccatct atataatatt actgatggcc gcgcggattt tggagccccc ttttgtcgtt
     4681 agcgcgacct ttaattcaaa ttaaaggttg cttttttcta tcgtccgatc attttgcgcc
     4741 tgactagcct agatattagc aacaacttcg cggctaagtt gttgaccgtc aagtataaat
     4801 taatgggctt atggcctaat gggtatagag caaaatggca tgcctgcaga ctagtgcttt
     4861 actctgatcc gcagatattt catcaggaaa gagctttgca agttccttca ttgtagcatc
     4921 aataatttct gagtcactac atgcaatcca ttcttctgct ggggcaaaaa ctaactccaa
     4981 catggattgg tttggattgt aatattcctt acatgttaca gacatgtcag cataaacact
     5041 taaaagcggg cttctgctaa agagtagatg atcataggtg ttcttcaatt tcctatcaaa
     5101 ccagatgtga acgttgataa ctggaactcc aactaatttc tctaatttct tgaagtatga
     5161 aatctctctc cagtcttcag gcactagtga attcgctagc gttaactggc cataatcctg
     5221 tgtatgcaac gttgaaaatt cggatctatt tttacgattc gatcatgaat taataaaatt
     5281 taaattttat tgaatgattt tctagtacat aactgacata cgacttatca gttgcgaaac
     5341 gaacagctct aattacattg ttaagtgaaa ttacgcctaa ttcgtctaag tacaacataa
     5401 ccaactgttt aaacctaatt aaataagtcg acccagaagc tgtcagagat gtcgtccaga
     5461 cttggaagtt caggtaagct ttgtggagat gcaacgctct ccgtaagttg tggttgaatc
     5521 ggatctggac gtggtatata cgcgtcctgg tgtatagcag atcctctact ccgtatatct
     5581 tgaaatatag gggatttatt atctcccaga tatacacgcc attctctgcc tgacgtgcag
     5641 tgatgatttc ccctgtgcgt gaatccatga ccggtgcagc ctatgtggaa gtatatggtg
     5701 cacccacagt ttagatcaat gcgtcgtcgt ctaatcgccc tcctcttggc ttgcctgtgt
     5761 gccttcttga tagagggggg cgtcgagagt gatgaagata acattcttga gagtccaatg
     5821 tttgagcgac gcattttccg ctttatccag gaaatcttta tagctggatc cctcgcctgg
     5881 attgcaaagc acgattgaag ggatccctcc tttaatttga actggtttcc cgtacttgca
     5941 atttgactgc cagtcttttt gggccccaag aagctctttc caatgcttta gctttaggta
     6001 ttgcggtgct acgtcatcaa tgacgttata ggcaacatta ttcgagtaca ctcgattatt
     6061 gaagtccagg tgtccactga ggtaattatg tgggcctaaa gcacgagccc acatcgtctt
     6121 gcccgttcta gaatcacctt ccactatgag actaactggt cttacaggcc gcgcagcggg
     6181 acccattcca aaataatcat ccgcccattc ttgcatctgt tcaggaacgt tagtgaaaga
     6241 ggaaagtgga aatggaggga cccattgttc aggcgccttt ttgaaaaggc gttccaagtt
     6301 cgctgtaatg ttatggtaac tcacgatgaa cgcccttgga tctccggccc ggataattgc
     6361 aagagcttcc gccaaactag gtgcattgat ggcgttataa tagacgtcgt ctttattctt
     6421 ctttgtaccc ccaggcactt tgaactgtcc ggattcacaa caatcgccgt ctttggtgat
     6481 gtaattcttg acggcgttgg cgtctttggc tgcttgtatg tttgggtgaa aattggtcga
     6541 ccttctgggg tgagtaaggt cgaaaaatct cttattcttg atgttggact ttccagagag
     6601 ttggatgaga cagtgaaggt gtgggaatcc gtccgagtgc tcctctctgg caactcgaat
     6661 atatgttggt ttgacgactg accatgataa ggtttgaagc atttgaagag cttcattttt
     6721 tgggatatcg cacttgggat aggttaagaa aatatgtctg gcttgtaaac gaaaagaatc
     6781 ggggtttcta ggcatttttt aaatagtgac ccaggactca aaaactagct ctcaacttct
     6841 ctcatatgtt gtggagtctg gagtccttta tatatgttta cgctatagaa ggcatttggg
     6901 gactctcccg ggcccaccat aggactccat accgcggcca tctatataat attactgatg
     6961 gccgcgcgga ttttggagcc cccttttgtc gttagcgcga cctttaattc aaattaaagg
     7021 ttgctttttt ctatcgtcca atcattttgc gcctgactag cctagatatt agcaacaact
     7081 tcgcgactaa gttgttgacc gtcaagtata aattaatggg cttatggccc aatgggtata
     7141 gagcaaaatg cccaagcgtg atgccccgtg gcgcttatta gcgggtacgt ctaaggtaag
     7201 tcgaaatgcc aactactctc ccttgagagt attgggccct aaagttaata agggacctcg
     7261 agggggggcc cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca
     7321 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7381 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7441 ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag
     7501 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
     7561 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     7621 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
     7681 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
     7741 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
     7801 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
     7861 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
     7921 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
     7981 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
     8041 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
     8101 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
     8161 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
     8221 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     8281 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     8341 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     8401 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     8461 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     8521 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     8581 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     8641 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
     8701 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     8761 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     8821 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     8881 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     8941 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     9001 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     9061 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     9121 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     9181 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     9241 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     9301 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     9361 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     9421 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     9481 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     9541 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     9601 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     9661 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     9721 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     9781 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     9841 gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac
     9901 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
     9961 gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa
    10021 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc
    10081 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
    10141 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
    10201 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
    10261 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc
    10321 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
    10381 cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac
    10441 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
    10501 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
    10561 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
    10621 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
    10681 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
    10741 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
    10801 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
    10861 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
    10921 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
    10981 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
    11041 ggtcgaaaag gtgtctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt
    11101 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg
    11161 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt
    11221 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa
    11281 gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt
    11341 cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca
    11401 gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg
    11461 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11521 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11581 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
    11641 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
    11701 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
    11761 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    11821 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    11881 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    11941 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    12001 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    12061 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    12121 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    12181 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    12241 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    12301 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    12361 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    12421 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    12481 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    12541 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac
    12601 taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc
    12661 agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg
    12721 acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag
    12781 ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag
    12841 acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta
    12901 aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag
    12961 taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg
    13021 atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt
    13081 tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc
    13141 cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat
    13201 agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc
    13261 atttttaaat ataggttttc attttctccc accagcttat ataccttagc aggagacatt
    13321 ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt
    13381 ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag
    13441 aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
    13501 accagaaaac agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg
    13561 agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac
    13621 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
    13681 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
    13741 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
    13801 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
    13861 acttaataac acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg
    13921 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
    13981 tgctccacca tgttggcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt
    14041 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    14101 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    14161 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    14221 atgacatgat tacg
//
