LOCUS       sequence-477703-       16506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant mitoTALEN vector to cut Brassica CMS gene orf125 (orf138).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16506)
  AUTHORS   Kazama T, Okuno M, Watari Y, Yanase S, Koizuka C, Tsuruta Y, Sugaya 
            H, Toyoda A, Itoh T, Tsutsumi N, Toriyama K, Koizuka N, Arimura SI
  TITLE     Curing cytoplasmic male sterility via TALEN-mediated mitochondrial 
            genome editing.
  JOURNAL   Nat Plants. 2019 Jul;5(7):722-730. doi: 10.1038/s41477-019-0459-z. 
            Epub 2019 Jul 8.
  PUBMED    31285556
REFERENCE   2  (bases 1 to 16506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-019-0459-z"; journalName: "Nat Plants"; date: 
            "2019-07"; volume: "5"; issue: "7"; pages: "722-730"
COMMENT     Sequence Label: pK7WG2-mitoTAL2-orf125
FEATURES             Location/Qualifiers
     source          1..16506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4981..5005
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        5089..5434
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    5454..5509
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    5706..5730
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5733..5759
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7803..8390
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     protein_bind    complement(8428..8448)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      8453..8701
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        9286..9631
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    9651..9706
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    9892..9912
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             9915..9959
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             12516..13103
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     protein_bind    complement(13137..13161)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        13533..13712
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             13746..14540
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13800..13819)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14410..14429
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      14580..14832
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    14925..14949
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             15470..16261
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttaa agtactttaa agtactttaa
     4861 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
     4921 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
     4981 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
     5041 aatctgatcc aagctcaagc taagctcctc agaagaccag agggctattg agacttttca
     5101 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     5161 cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
     5221 ggctatcgtt caagatgcct ctaccgacag tggtcccaaa gatggacccc cacccacgag
     5281 gaacatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     5341 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
     5401 tatataagga agttcatttc atttggagag gacaggcttc ttgagatcct tcaacaatta
     5461 ccaacaacaa caaacaacaa acaacattac aattactatt tacaattaca gtcgacccac
     5521 gcgtccgata atttccccaa tttctcagag agactcaaaa tcaccaccac caccaaatcg
     5581 ccgccatgtt taaacaagct tctcgtctcc tctcccgatc tgtcgccgcc gcatcttcca
     5641 aatcggtgac gactcgtgcc ttttcaacgg aacttccatc gacgctcgat tccgctagtg
     5701 atatcacaag tttgtacaaa aaagcaggct tttacccata cgatgttcca gattacgctg
     5761 atatcgccga tctacgcacg ctcggctaca gccagcagca acaggagaag atcaaaccga
     5821 aggttcgttc gacagtggcg cagcaccacg aggcactggt cggccacggg tttacacacg
     5881 cgcacatcgt tgcgttaagc caacacccgg cagcgttagg gaccgtcgct gtcaagtatc
     5941 aggacatgat cgcagcgttg ccagaggcga cacacgaagc gatcgttggc gtcggcaaac
     6001 agtggtccgg cgcacgcgct ctggaggcct tgctcacggt ggcgggagag ttgagaggtc
     6061 caccgttaca gttggacaca ggccaacttc tcaagattgc aaaacgtggc ggcgtgaccg
     6121 cagtggaggc agtgcatgca tggcgcaatg cactgacggg tgccccgctc aacctgaccc
     6181 ccgaccaggt tgtcgctatt gctagtaacg gcggaggcaa acaggcgctg gaaacagttc
     6241 agcgcctctt gccggtcttg tgtcaggacc acggcctgac cccagaacaa gttgtcgcga
     6301 ttgcaagcaa caacggaggc aaacaagcct tagaaacagt ccagagattg ttgccggtgc
     6361 tgtgccaagc ccacggcctg acccccgacc aggttgtcgc tattgctagt aacggcggag
     6421 gcaaacaggc gctggaaaca gttcagcgcc tcttgccggt cttgtgtcag gcccacggcc
     6481 tgaccccggc ccaggtggtt gcaatcgcgt cacacgatgg gggaaagcag gccctagaaa
     6541 ccgttcagcg actcctgccc gtcctgtgcc aggaccacgg cctgaccccg gaccaggtgg
     6601 ttgcaatcgc gtcacacgat gggggaaagc aggccctaga aaccgttcag cgactcctgc
     6661 ccgtcctgtg ccaggaccac ggcctgaccc cagaacaggt tgtggccatc gccagcaaca
     6721 taggtggcaa gcaggccctc gaaaccgtcc agagactgtt accggttctc tgccaggccc
     6781 acggcctgac cccggaccag gtggttgcaa tcgcgtcaca cgatggggga aagcaggccc
     6841 tagaaaccgt tcagcgactc ctgcccgtcc tgtgccaggc ccacggcctg acccccgccc
     6901 aggttgtcgc tattgctagt aacggcggag gcaaacaggc gctggaaaca gttcagcgcc
     6961 tcttgccggt cttgtgtcag gaccacggcc tgacccccga ccaggttgtc gctattgcta
     7021 gtaacggcgg aggcaaacag gcgctggaaa cagttcagcg cctcttgccg gtcttgtgtc
     7081 aggaccacgg cctgaccccc gaacaggttg tcgctattgc tagtaacggc ggaggcaaac
     7141 aggcgctgga aacagttcag cgcctcttgc cggtcttgtg tcaggcccac ggcctgaccc
     7201 ccgaccaggt tgtcgctatt gctagtaacg gcggaggcaa acaggcgctg gaaacagttc
     7261 agcgcctctt gccggtcttg tgtcaggccc acggcctgac ccccgcccag gttgtcgcta
     7321 ttgctagtaa cggcggaggc aaacaggcgc tggaaacagt tcagcgcctc ttgccggtct
     7381 tgtgtcagga ccacggcctg accccagacc aagttgtcgc gattgcaagc aacaacggag
     7441 gcaaacaagc cttagaaaca gtccagagat tgttgccggt gctgtgccaa gaccacggcc
     7501 tgacccccga acaggttgtc gctattgcta gtaacggcgg aggcaaacag gcgctggaaa
     7561 cagttcagcg cctcttgccg gtcttgtgtc aggcccacgg cctgacccct gagcaggtag
     7621 tggctattgc atcccacgac gggggcagac ccgcactgga gtcaattgtt gcccagttat
     7681 ctcgccctga tccggcgttg gccgcgttga ccaacgacca cctcgtcgcc ttggcctgcc
     7741 tcggcgggcg tcctgcgctg gatgcagtga aaaagggatt gggggatcct atcagccgtt
     7801 cccagctggt gaagtccgag ctggaggaga agaaatccga gttgaggcac aagctgaagt
     7861 acgtgcccca cgagtacatc gagctgatcg agatcgcccg gaacagcacc caggaccgta
     7921 tcctggagat gaaggtgatg gagttcttca tgaaggtgta cggctacagg ggcaagcacc
     7981 tgggcggctc caggaagccc gacggcgcca tctacaccgt gggctccccc atcgactacg
     8041 gcgtgatcgt ggacaccaag gcctactccg gcggctacaa cctgcccatc ggccaggccg
     8101 acgaaatgca gaggtacgtg gaggagaacc agaccaggaa caagcacatc aaccccaacg
     8161 agtggtggaa ggtgtacccc tccagcgtga ccgagttcaa gttcctgttc gtgtccggcc
     8221 acttcaaggg caactacaag gcccagctga ccaggctgaa ccacatcacc aactgcaacg
     8281 gcgccgtgct gtccgtggag gagctcctga tcggcggcga gatgatcaag gccggcaccc
     8341 tgaccctgga ggaggtgagg aggaagttca acaacggcga gatcaacttc gcggccgact
     8401 gataactcga gcgatcctct agacacccaa cttttctata caaagttgtg aatatgaaga
     8461 tgaagatgaa atatttggtg tgtcaaataa aaagcttgtg tgcttaagtt tgtgtttttt
     8521 tcttggcttg ttgtgttatg aatttgtggc tttttctaat attaaatgaa tgtaagatct
     8581 cattataatg aataaacaaa tgtttctata atccattgtg aatgttttgt tggatctctt
     8641 ctgcagcata taactactgt atgtgctatg gtatggacta tggaatatga ttaaagataa
     8701 gatgggctca tagagtaaaa cgaggcgagg gacctataaa cctcccttca tcatgctatt
     8761 tcatgatcta ttttataaaa taaagatgta gaaaaaagta agcgtaataa ccgcaaaaca
     8821 aatgatttaa aacatggcac ataatgagga gattaagttc ggtttacgtt tattttagta
     8881 ctaattgtaa cgtgagacta cgtatcggga atcgcctaat taaagcatta atgcgaacct
     8941 gattagattc accgaccctc ctatcgtgtc gacctttctg tttcttagaa ttttttggta
     9001 gtctatgtac taataatgtc agcttcgtat ttatttcata agcaatttgc atttgcaatt
     9061 tgttttttac ttttattttt attgtattgt ggaatgtgga ctcgtaccaa catgaagtta
     9121 tataccacca aaaaaattac agttagtcaa aagattcacg agtgagagct acttatgatt
     9181 gtcttttacg tatatgtcta attgtctatt tgctcaataa tctttgtact ttcttttgtc
     9241 gttgataaat ctagaggatc ccccctcaga agaccagagg gctattgaga cttttcaaca
     9301 aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga
     9361 aaggacagta gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
     9421 tatcgttcaa gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa
     9481 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
     9541 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
     9601 ataaggaagt tcatttcatt tggagaggac aggcttcttg agatccttca acaattacca
     9661 acaacaacaa acaacaaaca acattacaat tactatttac aattacagtc gacccacgcg
     9721 tccgataatt tccccaattt ctcagagaga ctcaaaatca ccaccaccac caaatcgccg
     9781 ccatgtttaa acaagcttct cgtctcctct cccgatctgt cgccgccgca tcttccaaat
     9841 cggtgacgac tcgtgccttt tcaacggaac ttccatcgac gctcgattcc acaactttgt
     9901 ataataaagt tgataaggag accgccgctg ccaagttcga gagacagcac atggacagca
     9961 tcgatatcgc cgatctacgc acgctcggct acagccagca gcaacaggag aagatcaaac
    10021 cgaaggttcg ttcgacagtg gcgcagcacc acgaggcact ggtcggccac gggtttacac
    10081 acgcgcacat cgttgcgtta agccaacacc cggcagcgtt agggaccgtc gctgtcaagt
    10141 atcaggacat gatcgcagcg ttgccagagg cgacacacga agcgatcgtt ggcgtcggca
    10201 aacagtggtc cggcgcacgc gctctggagg ccttgctcac ggtggcggga gagttgagag
    10261 gtccaccgtt acagttggac acaggccaac ttctcaagat tgcaaaacgt ggcggcgtga
    10321 ccgcagtgga ggcagtgcat gcatggcgca atgcactgac gggtgccccg ctcaacctga
    10381 cccccgacca ggttgtcgct attgctagta acggcggagg caaacaggcg ctggaaacag
    10441 ttcagcgcct cttgccggtc ttgtgtcagg accacggcct gaccccagaa caggttgtgg
    10501 ccatcgccag caacataggt ggcaagcagg ccctcgaaac cgtccagaga ctgttaccgg
    10561 ttctctgcca ggcccacggc ctgaccccgg accaggtggt tgcaatcgcg tcacacgatg
    10621 ggggaaagca ggccctagaa accgttcagc gactcctgcc cgtcctgtgc caggcccacg
    10681 gcctgacccc agcccaggtt gtggccatcg ccagcaacat aggtggcaag caggccctcg
    10741 aaaccgtcca gagactgtta ccggttctct gccaggacca cggcctgacc ccggaccagg
    10801 tggttgcaat cgcgtcacac gatgggggaa agcaggccct agaaaccgtt cagcgactcc
    10861 tgcccgtcct gtgccaggac cacggcctga cccccgaaca ggttgtcgct attgctagta
    10921 acggcggagg caaacaggcg ctggaaacag ttcagcgcct cttgccggtc ttgtgtcagg
    10981 cccacggcct gaccccagac caggttgtgg ccatcgccag caacataggt ggcaagcagg
    11041 ccctcgaaac cgtccagaga ctgttaccgg ttctctgcca ggcccacggc ctgaccccag
    11101 cccaggttgt ggccatcgcc agcaacatag gtggcaagca ggccctcgaa accgtccaga
    11161 gactgttacc ggttctctgc caggaccacg gcctgacccc ggaccaggtg gttgcaatcg
    11221 cgtcacacga tgggggaaag caggccctag aaaccgttca gcgactcctg cccgtcctgt
    11281 gccaggacca cggcctgacc cccgaacagg ttgtcgctat tgctagtaac ggcggaggca
    11341 aacaggcgct ggaaacagtt cagcgcctct tgccggtctt gtgtcaggcc cacggcctga
    11401 cccccgacca ggttgtcgct attgctagta acggcggagg caaacaggcg ctggaaacag
    11461 ttcagcgcct cttgccggtc ttgtgtcagg cccacggcct gacccccgcc caggttgtcg
    11521 ctattgctag taacggcgga ggcaaacagg cgctggaaac agttcagcgc ctcttgccgg
    11581 tcttgtgtca ggaccacggc ctgaccccag accaggttgt ggccatcgcc agcaacatag
    11641 gtggcaagca ggccctcgaa accgtccaga gactgttacc ggttctctgc caggaccacg
    11701 gcctgacccc ggaacaggtg gttgcaatcg cgtcacacga tgggggaaag caggccctag
    11761 aaaccgttca gcgactcctg cccgtcctgt gccaggccca cggcctgacc ccagaccagg
    11821 ttgtggccat cgccagcaac ataggtggca agcaggccct cgaaaccgtc cagagactgt
    11881 taccggttct ctgccaggcc cacggcctga cccccgccca ggttgtcgct attgctagta
    11941 acggcggagg caaacaggcg ctggaaacag ttcagcgcct cttgccggtc ttgtgtcagg
    12001 accacggcct gacccccgac caggttgtcg ctattgctag taacggcgga ggcaaacagg
    12061 cgctggaaac agttcagcgc ctcttgccgg tcttgtgtca ggaccacggc ctgaccccgg
    12121 aacaggtggt tgcaatcgcg tcacacgatg ggggaaagca ggccctagaa accgttcagc
    12181 gactcctgcc cgtcctgtgc caggcccacg gcctgacccc agaccaggtt gtggccatcg
    12241 ccagcaacat aggtggcaag caggccctcg aaaccgtcca gagactgtta ccggttctct
    12301 gccaggccca cggcctgacc cctgagcagg tagtggctat tgcatccaac aacgggggca
    12361 gacccgcact ggagtcaatt gttgcccagt tatctcgccc tgatccggcg ttggccgcgt
    12421 tgaccaacga ccacctcgtc gccttggcct gcctcggcgg gcgtcctgcg ctggatgcag
    12481 tgaaaaaggg attgggggat cctatcagcc gttcccagct ggtgaagtcc gagctggagg
    12541 agaagaaatc cgagttgagg cacaagctga agtacgtgcc ccacgagtac atcgagctga
    12601 tcgagatcgc ccggaacagc acccaggacc gtatcctgga gatgaaggtg atggagttct
    12661 tcatgaaggt gtacggctac aggggcaagc acctgggcgg ctccaggaag cccgacggcg
    12721 ccatctacac cgtgggctcc cccatcgact acggcgtgat cgtggacacc aaggcctact
    12781 ccggcggcta caacctgccc atcggccagg ccgacgaaat gcagaggtac gtggaggaga
    12841 accagaccag gaacaagcac atcaacccca acgagtggtg gaaggtgtac ccctccagcg
    12901 tgaccgagtt caagttcctg ttcgtgtccg gccacttcaa gggcaactac aaggcccagc
    12961 tgaccaggct gaaccacatc accaactgca acggcgccgt gctgtccgtg gaggagctcc
    13021 tgatcggcgg cgagatgatc aaggccggca ccctgaccct ggaggaggtg aggaggaagt
    13081 tcaacaacgg cgagatcaac ttcgcggccg actgataact cgagcgatcc tctagaaccc
    13141 agctttcttg tacaaagtgg tgatatcccg cggccatgct agagtccgca aaaatcacca
    13201 gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc
    13261 cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
    13321 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
    13381 caaaatccag tgacctgcag gcatgcgacg tcgggccctc tagaggatcc ccgggtaccg
    13441 cgaattatcg atcatgagcg gagaattaag ggagtcacgt tatgaccccc gccgatgacg
    13501 cgggacaagc cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactgag
    13561 ccgcgggttt ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt
    13621 caaaagtcgc ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg
    13681 acgttccata aattcccctc ggtatccaat tagagtctca tattcactct caactcgatc
    13741 gaggcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
    13801 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
    13861 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
    13921 aactccaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
    13981 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
    14041 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
    14101 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
    14161 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
    14221 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcggatgc
    14281 ccgacggcga ggatctcgtc gtgacccacg gcgatgcctg cttgccgaat atcatggtgg
    14341 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
    14401 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
    14461 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
    14521 ttcttgacga gttcttctga gcgggactct ggggttcgga ctctagctag agtcaagcag
    14581 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
    14641 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
    14701 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
    14761 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
    14821 tgttactaga tcgaccggca tgcaagctga taattcaatt cggcgttaat tcagtacatt
    14881 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
    14941 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
    15001 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
    15061 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
    15121 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
    15181 gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
    15241 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
    15301 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
    15361 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
    15421 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
    15481 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
    15541 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
    15601 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
    15661 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
    15721 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
    15781 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
    15841 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
    15901 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
    15961 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
    16021 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
    16081 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
    16141 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
    16201 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
    16261 atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg
    16321 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat
    16381 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa
    16441 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    16501 atcttc
//
