LOCUS       sequence-477705-       20411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to specifically silence the GhCLA 
            gene via virus-induced gene silencing (VIGS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20411)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 20411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVS2-GhCLA
FEATURES             Location/Qualifiers
     source          1..20411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1276..1293
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1328..1580
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1589..1605)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1589..1605)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    1626..1650
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2115..2765
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(2953..2970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3204..3223)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            3446..3555
                     /note="incP origin of transfer"
     CDS             3588..3959
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      4086..4797
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  5420..6187
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             6379..7173
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             7472..8620
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKTYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    10216..10240
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(10487..10508)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10699..10718
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10843..10865
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10857..10874
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10858..10874
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10883..11135)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(17989..18334)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        18864..18910
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 acgctgtttg agggaaaagt agagaacgta tttgttttta tgttcaggcg gttcttgtgt
       61 gtcaacaaag atggacattg ttactcaagg aagcacgatg agctttatta ttacggacga
      121 gtggacttag attctgtgag taaggttacc gaattggcca gttaacgcta gcgaatcgag
      181 accgcccttt gtgcatcttc atttcctgcc attatcaact ggggtgcagc ttcagatcct
      241 aaaaaatcca ctccttttgc ttctcatttt cttggtggat cagatctggt tttgcagcca
      301 ttgaagaagc tcaatcaggt caagaaaagg ccaggtgggg tttatgcatc actatcagaa
      361 ggagctgaat atcactccca aagaccagca acgcctctct tggacactat aaactatcca
      421 attcatatga aaaatctctc tgtcaaggaa ctgaaacaac tatctgaaga actgcggtct
      481 gatgtgattt tcaatgtttc aaaaactggg ggtcacttgg gttcaagcct tggtgtggtt
      541 gaactcactg tggctcttca ttatgtcttc aatgccccta gagacaagat attgtgggat
      601 gttggtcatc agtcttaccc tcacaaaatc ttgactggga gaagacataa gatgcatacc
      661 atgaggcaaa ctaatggatt ggccggattc acgaaacggt cggagagtga atacgattgc
      721 ttcgggactg gtcacagttc aaccacaatc tctgcaggct tagccgcaac ggtgttaatg
      781 gtctcaacta gtctgcaggc atgccattcg aggcccgggc atgtcccgaa gacattaaac
      841 tacggttctt taagtagatc cgtgtctgaa gttttaggtt caatttaaac ctacgagatt
      901 gacattctcg actgatcttg attgatcggt aagtcttttg taatttaatt ttctttttga
      961 ttttatttta aattgttatc tgtttctgtg tatagactgt ttgagatcgg cgtttggccg
     1021 actcattgtc ttaccatagg ggaacggact ttgtttgtgt tgttatttta tttgtatttt
     1081 attaaaattc tcaacgatct gaaaaagcct cgcggctaag agattgttgg ggggtgagta
     1141 agtactttta aagtgatgat ggttacaaag gcaaaagggg taaaacccct cgcctacgta
     1201 agcgttatta cgcccgtctg tacttatatc agtacactga cgagtcccta aaggacgaaa
     1261 cgggagaacg ctagccacca ccaccaccac cacgtgtgaa ttacaggtga ccagctcgaa
     1321 tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     1381 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     1441 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     1501 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     1561 gtcatctatg ttactagatc gggaattggt catagctgtt tcctgggatc caaactatca
     1621 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
     1681 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     1741 ccacagggtt ccccagatct ggcgccggcc agcgagacga gcaagattgg ccgccgcccg
     1801 aaacgatccg acagcgcgcc cagcacaggt gcgcaggcaa attgcaccaa cgcatacagc
     1861 gccagcagaa tgccatagtg ggcggtgacg tcgttcgagt gaaccagatc gcgcaggagg
     1921 cccggcagca ccggcataat caggccgatg ccgacagcgt cgagcgcgac agtgctcaga
     1981 attacgatca ggggtatgtt gggtttcacg tctggcctcc ggaccagcct ccgctggtcc
     2041 gattgaacgc gcggattctt tatcactgat aagttggtgg acatattatg tttatcagtg
     2101 ataaagtgtc aagcatgaca aagttgcagc cgaatacagt gatccgtgcc gccctggacc
     2161 tgttgaacga ggtcggcgta gacggtctga cgacacgcaa actggcggaa cggttggggg
     2221 ttcagcagcc ggcgctttac tggcacttca ggaacaagcg ggcgctgctc gacgcactgg
     2281 ccgaagccat gctggcggag aatcatacgc attcggtgcc gagagccgac gacgactggc
     2341 gctcatttct gatcgggaat gcccgcagct tcaggcaggc gctgctcgcc taccgcgatg
     2401 gcgcgcgcat ccatgccggc acgcgaccgg gcgcaccgca gatggaaacg gccgacgcgc
     2461 agcttcgctt cctctgcgag gcgggttttt cggccgggga cgccgtcaat gcgctgatga
     2521 caatcagcta cttcactgtt ggggccgtgc ttgaggagca ggccggcgac agcgatgccg
     2581 gcgagcgcgg cggcaccgtt gaacaggctc cgctctcgcc gctgttgcgg gccgcgatag
     2641 acgccttcga cgaagccggt ccggacgcag cgttcgagca gggactcgcg gtgattgtcg
     2701 atggattggc gaaaaggagg ctcgttgtca ggaacgttga aggaccgaga aagggtgacg
     2761 attgatcagg accgctgccg gagcgcaacc cactcactac agcagagcca tgtagacaac
     2821 atcccctccc cctttccacc gcgtcagacg cccgtagcag cccgctacgg gctttttcat
     2881 gccctgccct agcgtccaag cctcacggcc gcgctcggcc tctctggcgg ccttctggcg
     2941 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3001 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3061 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3121 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3181 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3241 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3301 aagcgtggcg cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg
     3361 tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc
     3421 cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg
     3481 ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc
     3541 tgcgaggctg gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa
     3601 ccaagccaac caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa
     3661 gagcgattga ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg
     3721 tcggccaggg ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc
     3781 gcatcaatgg cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc
     3841 cgcgcacggc gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga
     3901 agcaggacga gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac
     3961 ttttttagcc gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc
     4021 tccatcaaga agagcgactt cgcggagctg gtgaagtaca tcaccgacga gcaaggcaag
     4081 accgagcgcc tttccgacgc tcaccgggct ggttgccctc gccgctgggc tggcggccgt
     4141 ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg
     4201 ccggcgttgt ggatacctcg cggaaaactt ggccctcact gacagatgag gggcggacgt
     4261 tgacacttga ggggccgact cacccggcgc ggcgttgaca gatgaggggc aggctcgatt
     4321 tcggccggcg acgtggagct ggccagcctc gcaaatcggc gaaaacgcct gattttacgc
     4381 gagtttccca cagatgatgt ggacaagcct ggggataagt gccctgcggt attgacactt
     4441 gaggggcgcg actactgaca gatgaggggc gcgatccttg acacttgagg ggcagagtgc
     4501 tgacagatga ggggcgcacc tattgacatt tgaggggctg tccacaggca gaaaatccag
     4561 catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt
     4621 taaaccaata tttataaacc ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg
     4681 cacgccgaag gggggtgccc ccccttctcg aaccctcccg gcccgctaac gcgggcctcc
     4741 catcccccca ggggctgcgc ccctcggccg cgaacggcct caccccaaaa atggcagcgc
     4801 tggcagtcct tgccattgcc gggatcgggg cagtaacggg atgggcgatc agcccgagcg
     4861 cgacgcccgg aagcattgac gtgccgcagg tgctggcatc gacattcagc gaccaggtgc
     4921 cgggcagtga gggcggcggc ctgggtggcg gcctgccctt cacttcggcc gtcggggcat
     4981 tcacggactt catggcgggg ccggcaattt ttaccttggg cattcttggc atagtggtcg
     5041 cgggtgccgt gctcgtgttc gggggtgcga taaacccagc gaaccatttg aggtgatagg
     5101 taagattata ccgaggtatg aaaacgagaa ttggaccttt acagaattac tctatgaagc
     5161 gccatattta aaaagctacc aagacgaaga ggatgaagag gatgaggagg cagattgcct
     5221 tgaatatatt gacaatactg ataagataat atatctttta tatagaagat atcgccgtat
     5281 gtaaggattt cagggggcaa ggcataggca gcgcgcttat caatatatct atagaatggg
     5341 caaagcataa aaacttgcat ggactaatgc ttgaaaccca ggacaataac cttatagctt
     5401 gtaaattcta tcataattgg gtaatgactc caacttattg atagtgtttt atgttcagat
     5461 aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagc gacttccgtc
     5521 ccagccgtgc caggtgctgc ctcagattca ggttatgccg ctcaattcgc tgcgtatatc
     5581 gcttgctgat tacgtgcagc tttcccttca ggcgggattc atacagcggc cagccatccg
     5641 tcatccatat caccacgtca aagggtgaca gcaggctcat aagacgcccc agcgtcgcca
     5701 tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg gagactgtca tacgcgtaaa
     5761 acagccagcg ctggcgcgat ttagccccga catagcccca ctgttcgtcc atttccgcgc
     5821 agacgatgac gtcactgccc ggctgtatgc gcgaggttac cgactgcggc ctgagttttt
     5881 taagtgacgt aaaatcgtgt tgaggccaac gcccataatg cgggctgttg cccggcatcc
     5941 aacgccattc atggccatat caatgatttt ctggtgcgta ccgggttgag aagcggtgta
     6001 agtgaactgc agttgccatg ttttacggca gtgagagcag agatagcgct gatgtccggc
     6061 ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac agctgataga
     6121 aacagaagcc actggagcac ctcaaaaaca ccatcataca ctaaatcagt aagttggcag
     6181 catcacccat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
     6241 tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag
     6301 tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa
     6361 agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc
     6421 gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg
     6481 gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc
     6541 tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca
     6601 aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat
     6661 ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg
     6721 tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat
     6781 agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg
     6841 gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt
     6901 ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc
     6961 aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg
     7021 gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa
     7081 gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata
     7141 aaatattata ttttactgga tgaattgttt tagtacctag atgtggcgca acgatgccgg
     7201 cgacaagcag gagcgcaccg acttcttccg catcaagtgt tttggctctc aggccgaggc
     7261 ccacggcaag tatttgggca aggggtcgct ggtattcgtg cagggcaaga ttcggaatac
     7321 caagtacgag aaggacggcc agacggtcta cgggaccgac ttcattgccg ataaggtgga
     7381 ttatctggac accaaggcac caggcgggtc aaatcaggaa taagggcaca ttgccccggc
     7441 gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc ggaagacata
     7501 caggcaagaa ctgatcgacg cggggttttc cgccgaggat gccgaaacca tcgcaagccg
     7561 caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc
     7621 tacggccaag atcgagcgcg acagcgtgca actggctccc cctgccctgc ccgcgccatc
     7681 ggccgccgtg gagcgttcgc gtcgtctcga acaggaggcg gcaggtttgg cgaagtcgat
     7741 gaccatcgac acgcgaggaa ctatgacgac caagaagcga aaaaccgccg gcgaggacct
     7801 ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga agcagcagat
     7861 caaggaaatg cagctttcct tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat
     7921 gccaaacgac acggcccgct ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga
     7981 ggcgctgcaa aacaaggtca ttttccacgt caacaaggac gtgaagatca cctacaccgg
     8041 cgtcgagctg cgggccgacg atgacgaact ggtgtggcag caggtgttgg agtacgcgaa
     8101 gcgcacccct atcggcgagc cgatcacctt cacgttctac gagctttgcc aggacctggg
     8161 ctggtcgatc aatggccggt attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc
     8221 gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca
     8281 ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga
     8341 ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg agaagtaccg
     8401 caagctgtcg ccgacggccc gacggatgtt cgactatttc agctcgcacc gggagccgta
     8461 cccgctcaag ctggaaacct tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa
     8521 gtggcgcgag caggtcggcg aagcctgcga agagttgcga ggcagcggcc tggtggaaca
     8581 cgcctgggtc aatgatgacc tggtgcattg caaacgctag ggccttgtgg ggtcagttcc
     8641 ggctgggggt tcagcagcca gcgctttact ggcatttcag gaacaagcgg gcactgctcg
     8701 acgcacttgc ttcgctcagt atcgctcggg acgcacggcg cgctctacga actgccgata
     8761 aacagaggat taaaattgac aattgtgatt aaggctcaga ttcgacggct tggagcggcc
     8821 gacgtgcagg atttccgcga gatccgattg tcggccctga agaaagctcc agagatgttc
     8881 gggtccgttt acgagcacga ggagaaaaag cccatggagg cgttcgctga acggttgcga
     8941 gatgccgtgg cattcggcgc ctacatcgac ggcgagatca ttgggctgtc ggtcttcaaa
     9001 caggaggacg gccccaagga cgctcacaag gcgcatctgt ccggcgtttt cgtggagccc
     9061 gaacagcgag gccgaggggt cgccggtatg ctgctgcggg cgttgccggc gggtttattg
     9121 ctcgtgatga tcgtccgaca gattccaacg ggaatctggt ggatgcgcat cttcatcctc
     9181 ggcgcactta atatttcgct attctggagc ttgttgttta tttcggtcta ccgcctgccg
     9241 ggcggggtcg cggcgacggt aggcgctgtg cagccgctga tggtcgtgtt catctctgcc
     9301 gctctgctag gtagcccgat acgattgatg gcggtcctgg gggctatttg cggaactgcg
     9361 ggcgtggcgc tgttggtgtt gacaccaaac gcagcgctag atcctgtcgg cgtcgcagcg
     9421 ggcctggcgg gggcggtttc catggcgttc ggaaccgtgc tgacccgcaa gtggcaacct
     9481 cccgtgcctc tgctcacctt taccgcctgg caactggcgg ccggaggact tctgctcgtt
     9541 ccagtagctt tagtgtttga tccgccaatc ccgatgccta caggaaccaa tgttctcggc
     9601 ctggcgtggc tcggcctgat cggagcgggt ttaacctact tcctttggtt ccgggggatc
     9661 tcgcgactcg aacctacagt tgtttcctta ctgggctttc tcagccccag atctggggtc
     9721 gatcagccgg ggatgcatca ggccgacagt cggaacttcg ggtccccgac ctgtaccatt
     9781 cggtgagcaa tggatagggg agttgatatc gtcaacgttc acttctaaag aaatagcgcc
     9841 actcagcttc ctcagcggct ttatccagcg atttcctatt atgtcggcat agttctcaag
     9901 atcgacagcc tgtcacggtt aagcgagaaa tgaataagaa ggctgataat tcggatctct
     9961 gcgagggaga tgatatttga tcacaggcag caacgctctg tcatcgttac aatcaacatg
    10021 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
    10081 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
    10141 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
    10201 ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact
    10261 taataacaca ttgcggacgt ttttaatgta ctggggtggt ttttcttttc accagtgaga
    10321 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
    10381 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttccgaaa tcggcaaaat
    10441 cccttataaa tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa
    10501 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
    10561 cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa
    10621 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
    10681 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgccattc aggctgcgca
    10741 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    10801 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    10861 aaacgacggc cagtgaattc ccgatctagt aacatagatg acaccgcgcg cgataattta
    10921 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
    10981 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
    11041 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
    11101 cttaagaaac tttattgcca aatgtttgaa cgatcgggga aattcgaggg gcccgtttcg
    11161 tcctttaggg actcgtcagt gtactgatat aagtacagac gggcgtaata acgcttacgt
    11221 aggcgagggg ttttacccct tttgcctttg taaccatcat cactttaaaa gtacttactc
    11281 accccccaat aatctcttag cctcgaggcc ttttcagatc attgagaatt ttaataaaat
    11341 acaaataaaa taacaacaca aacaaagtcc gttcccctat ggtaagacaa tgagtcggcc
    11401 aagcgccaat ctcaaacagt ctatatacag aaacagataa caatatataa taaaatcaaa
    11461 aagcaaacaa acgatcaatc ccgaaaggaa catcatctct taaaaatctt tttggtgtcc
    11521 caaattctct gtcctctttg aatttcttct tagaggtctt cgaattatga gactttttag
    11581 ctgtcgcaga catgttttca gcttgatttc gtcgaatttg tttccaaatt tctcgatttc
    11641 ttgtttcaac acgtttacga cacttttcaa ggtgactacg gccacaacaa ttatacacat
    11701 caaaagtaaa atcattgata acaacacaga caaaccatcc acaattattt tccgcacacc
    11761 tacgtgtgac accaaccatg tcagcaactt gctttcgcaa tttgttagca tgaccgatac
    11821 tacacgtctc gtgaccaaga acagtgactt cattcacaca acccttgagt acacacgtca
    11881 tattgacaat aaatttcttt atgcattaag acgagttttt cttattagac gctctcttaa
    11941 catcttcact tgacaataat gacttaggtc tagtagtagc tggtctgtaa ggaccatcgt
    12001 acttcgcctc aatcgtcttc atctccactt gtttcaaaga catgaactca ccgaaaacct
    12061 cttgatcggt aaaatcgcct tcaatgtaat ccgaactgtt cagttcttcc acattgaggc
    12121 gcctagtcga atcagtagca acccatatta gcgacacttc gatactgaaa gggtatgtac
    12181 cgtcacagac cggaacgccc tcagaaatac aacccacctt ccagacatca agtacgttct
    12241 cctgtgtgga aatactgtat tcaaccttgt actgcgcaac tgaacagtta tcgcaagctc
    12301 gagctttgaa tctctgaata gtctttcctc tctttctttt ctcagtaagt ctcgaatcca
    12361 acagatcgat ttgaatgaac ccaggcgtat ctgcaggtag atgtgatgtt acaaccaatt
    12421 gtacacctac tatctttaga aacgcgtaag atttaatcat tttgtaatcc ctttggtcaa
    12481 aaacgtctaa cttgtgttca ctcttcttcc caccaacatg atttaggaca gtttctcttc
    12541 ttctgtgaaa atacttcggc ctcttcctcc taccgtccac aaacaattca atggccccta
    12601 gatttgagaa ttttgagact tcgaaagtct tcttcttcaa ggtgaccaat gacttgtctt
    12661 ccatattacc agtcgtaaaa gtttgacaca gctctctgtg ccttcttcca atcaacctta
    12721 gccgggttca attccttatc gttttcgtct cggaataatg tacacagagc tgtaaaactc
    12781 ttaatatgct tccatagcgc acaaagcgca tgaacagaat cacctttgta caaataccgg
    12841 tctgaaacag actcggacag tttggatacc accatgtagt tcccaagagc tctattggaa
    12901 tcattcagcg agatgtagat ctcggctaaa tgttgcacat cctttatact ctttttcccc
    12961 aacttcgtca gaacttttac cggatctggc acgaactcgt aacacgatga cgtcttaagc
    13021 aagaacttcc cacaaaacat tgggacatcg tacttaaaaa tcttgcactc gaaattccac
    13081 ttagtagcca actttggaca cggatcaaca aactgcgttc ctctaggaaa cgcaatcagt
    13141 gagtcatctc ctccatatgt aaccatgact gctttctcca atggtaattc agacaagagt
    13201 gcacacagtg ttctatctga atttgcatta taagtatcag catctccact cttttgttgg
    13261 taccaaatat gcgccatcat accattttga atatctctca cagtagtttg agtgtgcgac
    13321 acctcccaaa ggaaggccgc ccactcatct agcccaaata acctgtaaat ctccagctgc
    13381 agttgtaaat ggaagcgatt tgccgacttg tcgaatttag acatgtctat ctcaacaaag
    13441 tcgtaagccg cttccgtatt taagaacttc actcgatcgt ttaaatcact cgatgtcatt
    13501 ctcgtgttga acacaaaatg tggttgcata gcatccaact tgcgttcatt aatttctttg
    13561 aaaattggac cgaacaacga gttgatcaac ttctcgtgat acacaacagt ctgtagagct
    13621 gagtattcaa attgcggtgt taaatcagtc ttaggtttca cgtcagattt gatcatatac
    13681 atgtaagacg aatagtctat ttcatgtaac tctctcacat ctgctactac cttagcctgt
    13741 accgctgtgc tttgatttct ccaccatctc tccatttgag ctctattgac aataggatca
    13801 gcccgaattt ttcccacatc gtagacaaca gatttcagct tcttcatcgt ctcttcgatt
    13861 agaactgtcg cgtgcacatt ttcttgtaga tcgggtgccg cttggttcct tttctgcaat
    13921 gctaacaaac tctccaactg agtagtcttc cttttgtcag gcatagcagt acgaataacc
    13981 ggtttcagaa acgtttcttt ttcagcaaac ttgtctttcc agtttccact tttgatcgta
    14041 acattgtcta atcgcaaatt gcaatcagtc gttgccacca aatacccgtc taggcttgag
    14101 tctcttaacg aatttcccgg aaacaaagcg tcgtaccaca tctccaagtc cgtaatcgta
    14161 cctgaatctg gaacggcgca aggcccttca tgatgtctaa agacatcaaa cctagaccga
    14221 aaccgtcata agaccgtctc agtaacaaaa aatcttgcca aagccgcctt cgtaagcgcg
    14281 gcactttccc tgatctcttt gcttacatcg tcctctttca cagtttcata cacaagtgat
    14341 tgtgtgtgac gcgacaacgc cacgattaag tactcccgac ctctagcgat tgagtcatcc
    14401 gtaggtttcg tcctgactag gactacatct ttgaatgtct ctccttgcga ctcgtgtact
    14461 gtactcactt tagctgccac tttcctcaat gccaacaaat ttaccaactc cttcttctca
    14521 gactgaagga aagttatgta ctgagcacca ggcttcaaag aaacctgttc tttagacaca
    14581 atcttcctca ccgtcatcga attagcagtc gcgttatgtg ttctgacatc tccagtatag
    14641 tacttgttca atacggcagc cacatctgct ggacttctgt aagtttctct tttttctgta
    14701 acaatgtcaa actttccgac cagactagaa aacctcaaat caacttgaga taccctaggc
    14761 ttgaaagaaa tttgattctg atctccttga cagatacatc gtttagcacc agctatctga
    14821 gcgcaaaagt acaccatacc agcatgggcc atgagagctt catcgaaatg caacacgtct
    14881 ccggttaaag aaccatcaac acaatgcatc aaaaaagaat caaccgtctt cactctcctt
    14941 ttcaatttgc atggaaaacc tttgctcgcg aatctctcga tcaagtcgtc ggttgctgct
    15001 ctcccagtag agagaaccac atcgacacaa ggattagctg agttgacaat cattgtcgac
    15061 ttaccacaac caggtacacc gtcaactaat tcaaaagaac agttaactgg tcctagatcc
    15121 agggcactta aattgtccaa gatcaacctg ttattgcaaa gatacgtctg atcgcaaact
    15181 atgacataat cactcatcct ttcgaccgcg aagtcgttga ccaacatacc tcgttcccaa
    15241 acagccactt ctaagtatcc cgaccaatca tccatacgtt tgtctggttg gaacacataa
    15301 gcatgttcag tgaccactgg acgtatgcac cagttcttca tctccacatc ccacactccc
    15361 agtccgtgcg ggtaattctg acctccagtt cgaataactc ctctcgaata gtcacgatac
    15421 agagacataa tttcatcatt ttgagcgact ctctccgcat ctaagtagta cagaaactct
    15481 cttctagcgt ccacagaatc gtctccactg acaaccggcc tttttggtaa tctctcacct
    15541 cccatcactt ggaagtaatc gacacgtttg accgtttttc ctttgctgac caaaggtttg
    15601 acagctggca acacgttctc aaatcctggt ggcgttaaag gtttacacaa cacctttgta
    15661 ccgcttgttt cccctctttc acattcagct gaaaaggctg aaacaacctc gtcaatttct
    15721 gcttcagtgt caataagacc actcagaaac tgctccatga tctcctcatc gtctaccttt
    15781 cttgaaactc caactctagc ttgggtcagc acgttactgt cttccaacaa cagacccagt
    15841 ccgcttcgag atgaaccgac caccgatggt ggagaagttc tcaccgaact gccagaatag
    15901 ttagagaatt ttgcacttag aaactcgtca gcgatttctt tcttctgctg caatagctta
    15961 tcagacttgg cttttacctc ggataaatct ggtaaaaccg tctcaccgga ctcagatgcc
    16021 gaatacagac tgctaaagaa atcgttgatt tcataatagc ggcacggatt ctcaacgtct
    16081 aagacatggg ccccccgttt gatatcaaac atatcacata gttttttcac cagtccatct
    16141 cggatgtctc gacgcataag cgtgattcct ccaatggcat acacaacact cagaaatact
    16201 atcaccttgg ttttgtgcga gttctcgtaa catagttctg gaccatttgg tgtgtccttc
    16261 gcccaggtta gtaccttctt tggatagaat ccaattagag taccaaccat gccaacgaat
    16321 ccgccgactg cgatcttgtt agcaaactgc ttccacagtt ttcttgatat taaacccagc
    16381 acatcagtca atgacgtgat aggtttcgtc tcagtcagaa ttgcttcacg caactccctc
    16441 atgacaggtc gcgccacttg ttccttcact agcaaagttt gagccaacaa ctgtaaatct
    16501 cgagaatcta cactttgctt gttcttcacg gccgccccgt ttatgaataa gacccaatta
    16561 tttgaactca agtatgattt aacattgctt atggtcagct gctggtcaga taacctagct
    16621 atgtaatcca aacacttgtc catgaattgt ttctctacaa acaggtcttt cttgaccaac
    16681 tctcgcgttg attcgaccag atcgaaaatt gggacaacaa ccatgttttc ccatctactg
    16741 atatatattc ttcggtagta ctcttgcgat gatagcgacc tcctcggaac tccagctatc
    16801 ctgtagatcg aaaaaagcat tgtgtctcct ctcacctgcc acggttcgaa gtagaacaca
    16861 ttcccttggt aactcactgg cttccccagt agatacttct tgtactcttc ccagtcatga
    16921 atgtaagaaa aggaaggatc tttctcaaaa ccgaaataga tcttcccgtt cttcttcatg
    16981 tagtaaccgt caacagaagg taatggacct tcgtccacta acatactttc aggagcaaaa
    17041 agcatagcag catgaaccac tttagttttc ttctcaacca tagtagccat caagtctttc
    17101 aacttgaagt catacaggct gtgaagagct atcgcatact gcgcgccttt cttcttacca
    17161 tcagaaaaac ccctacatgt acaaccctga tatgtattat tgcacctaac cgcgttaggc
    17221 gcagcaatat tttcacgaaa ttggtcatac atccacagct cattctccga catctgtctc
    17281 ttaccacttc tagcttgatc accaatagca gtaagaaata tctgtcgaga aatttctctc
    17341 tgatcatccc gaatatctag atttggacaa caggaatgaa tccttttctc acctctagtt
    17401 acccaaaacg accattgacc accgatgtca tccacctctt gcagagcagg aactctatct
    17461 aaaagcaata acgcttgtag ttttcgctca gcagccgcaa acccatgaac catgtttttg
    17521 tcgtctttaa acacgatatt gaattcagga taaatttcca tcaacttgtt ctgttccttc
    17581 tgagaaagaa cttcatgaac aatcactgga tctttagtct ttctttctct aatcatgtca
    17641 tcgactttat caacaacaac tctttgcatc atttgcccga tctcacaatc aggtttagtc
    17701 agcatcaagt caaagaaatg actcctctgc tcagcagaca tctcgtccac attgagcaat
    17761 tgagacaact tgaagttacc gttcgccatt gtatctgtat gtatttttac aaagtatcaa
    17821 agaaaaggtt ttaggcacac cagtcacttt cccccactcg gtactaagct ggatagatta
    17881 aaccaaaaag aaaaacaaaa taaaactgta ggtgataagt acgttctacc gcgttctcac
    17941 caaaatccaa ttagcacgtt ctaccgcgtt caaaggattg aaatgtttta tcctctccaa
    18001 atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc
    18061 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
    18121 gtcttctttt tccacgatgt tcctcgtggg tgggggtcca tctttgggac cactgtcggt
    18181 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt agaagccatc
    18241 ttccttttct actgtccttt cgatgaagtg acagatagct gggcaatgga atccgaggag
    18301 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctctggtctt ctgagactgt
    18361 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttga ccatcacatc
    18421 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ttcctcgtgg
    18481 gtgggggtcc atctttggga ccactgtcgg tagaggcatc ttgaacgata gcctttcctt
    18541 tatcgcaatg atggcatttg tagaagccat cttccttttc tactgtcctt tcgatgaagt
    18601 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
    18661 tctcaatagc cctctggtct tctgagactg tatctttgat attcttggag tagacgagag
    18721 tgtcgtgctc caccatgttg acctgcaggc atgcgcaaat ttaaagcgct gatatcgatc
    18781 gcgcgcagat ctgtcatgat gatcattgca attggatcca tctccactga cgtaagggat
    18841 gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat
    18901 ttggagagga taaaacattg cacctatggt gttgccctgg ctggggtatg tcagtgatcg
    18961 cagtagaatg tactaattga caagttggag aatacggtag aacgtcctta tccaacacag
    19021 cctttatccc tctccctgac gaggtttttg tcagtgtaat atttcttttt gaactatcca
    19081 gcttagtacc gtacgggaaa gtgactggtg tgcttatctt tgaaatgtta ctttgggttt
    19141 cggttcttta ggttagtaag aaagcacttg tcttctcata caaaggaaaa cctgagacgt
    19201 atcgcttacg aaagtagcaa tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg
    19261 atggggtcgt tttaattaac ttctcctacg caagcgtcta aacggacgtt ggggttttgc
    19321 tagtttcttt agagaaaact agctaagtct ttaatgttat cattagagat ggcataaata
    19381 taatacttgt gtctgctgat aagatcattt taatttggac gattagactt gttgaactac
    19441 aggttactga atcacttgcg ctaatcaaca tgggagatat gtacgatgaa tcatttgaca
    19501 agtcgggcgg tcctgctgac ttgatggacg attcttgggt ggaatcagtt tcgtggaaag
    19561 atctgttgaa gaagttacac agcataaaat ttgcactaca gtctggtaga gatgagatca
    19621 ctgggttact agcggcactg aatagacagt gtccttattc accatatgag cagtttccag
    19681 ataagaaggt gtatttcctt ttagactcac gggctaacag tgctcttggt gtgattcaga
    19741 acgcttcagc gttcaagaga cgagctgatg agaagaatgc agtggcgggt gttacaaata
    19801 ttcctgcgaa tccaaacaca acggttacga cgaaccaagg gagtactact actaccaagg
    19861 cgaacactgg ctcgactttg gaagaagact tgtacactta ttacaaattc gatgatgcct
    19921 ctacagcttt ccacaaatct ctaacttcgt tagagaacat ggagttgaag agttattacc
    19981 gaaggaactt tgagaaagta ttcgggatta agtttggtgg agcagctgct agttcatctg
    20041 caccgcctcc agcgagtgga ggtccgatac gtcctaatcc caagctttag ggatttaagg
    20101 acgtgaactc tgttgagatc tctgtgaaat tcagagggtg ggtgatacca tattcactga
    20161 tgccattagc gacatctaaa tagggctaat tgtgactaat ttgagggaat ttcctttacc
    20221 attgacgtca gtgtcgttgg tagcatttga gtttcgcaat gcacgaatta cttaggaagt
    20281 ggcttgacga cactaatgtg ttattgttag ataatggttt ggtggtcaag gtacgtagta
    20341 gagtcccaca tattcgcacg tatgaagtaa ttggaaagtt gtcagttttt gataattcac
    20401 tgggagatga t
//
