LOCUS       sequence-477834-       11787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), 
            constiutive NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11787)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 11787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM764
FEATURES             Location/Qualifiers
     source          1..11787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             326..1408
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(345..364)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             join(4683..4751,4752..5543)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTSLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4901..4920)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            5674..5783
                     /note="incP origin of transfer"
     rep_origin      5954..6342
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    7274..7290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    7307..7323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(7368..7394,7395..7397,7398..9884,9885..9887,
                     9888..11474)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGAPLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGEFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        11634..11709
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atagccttga tctggaagaa gcaatgaaag ctgctgttaa gtctccgaat caggtattgt
       61 tcctgacagg tgtattccca tccggtaaac gcggatactt tgcagttgat ctgactcagg
      121 aataaattat aaattaaggt aagaagattg taggataagc taatgaaata gaaaaaggat
      181 gccgtcacac aacttgtcgg cattcttttt tgttttatta gttgaaaata tagtgaaaaa
      241 gttgcctaaa tatgtatgtt aacaaattat ttgtcgtaac tttgcactcc aaatctgttt
      301 ttaacatatg gcactagtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
      361 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
      421 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
      481 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
      541 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
      601 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
      661 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
      721 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
      781 ccagacaccc atcaacagta ttattttctc ccatgaggac ggtacgcgac tgggcgtgga
      841 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
      901 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc
      961 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
     1021 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg
     1081 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg
     1141 atacgacgat accgaggaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga
     1201 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
     1261 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc
     1321 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     1381 ggtttcccga ctggaaagcg ggcagtgagc tttcctcggt accaaattcc agaaaagagg
     1441 cctcccgaaa ggggggcctt ttttcgtttt ggtcctactt gtgcctgttc tatttccgaa
     1501 ccgaccgctt gtatgaatgc atcaaaattc gttttctcta cgttggattc cttgttgctc
     1561 atattgtgat gataatttct acaaatatag tcattggtaa ctatctatga aactgtttga
     1621 tacttttatc agtccagtag ttttacaagg tctttcttca tttcttcgtc aatatccctg
     1681 tatcgtctga aagctttgct tccctccttg tgtcccgaca gtgcggaaac aaggttcggg
     1741 tctttcactt ttttatagat attgccgata aacgtacgtc ttgccagatg gctgcttgcc
     1801 acttcataaa taggtcgttt gatttcgttg tgcgtcaacg ggtctaagat tgttacgatg
     1861 cggtcaactc cagctaattt gaatatcttt tttatggcat cattgtactt ttgctcggat
     1921 atgaacggca acagttttcc ctcatattct ttgtagcgtt caaggatttc tttcgctttg
     1981 tcgttaagtg gaacacgtac cgtaaccgga ttcccctctt tggttttctt gggaatatat
     2041 tctatggctt cattgaccac atttagtttg gtcattcggt acaggtcgct caccctgcat
     2101 cctatcagtg tctgaaatat gaatatatcc ctctgtattg ccagttgtgg ggtggcagaa
     2161 aggtctgcat taaaaatcct gtccctttct tcgagtgtta tataataagg tgtaccatat
     2221 gtacactcct ctatcggaaa cttgtcgaaa ggtctgtttg tggtgcgttt gttatcgaag
     2281 caccacagga agaatgtgcg tattcttgaa aaacagtcta tcagcgtgtt tttgcttctg
     2341 ggctgtggtg tcctcttttc gggaatggct tcataaatgc tcgggtaaag ttcataatac
     2401 tggtattcgt tctgaaagaa atcccacata tcccgaagcg tgtcaggtgt taccaaatcc
     2461 acatcaagga taaagccctt ttgtcctctc tttgtagccc ttacatatag ttcataacgc
     2521 agtaaggctc ttttgacaac ccggaaattt ttctttcgta cttccgacaa agggtgcttg
     2581 tttagaaatt catcgaatag ttctccaatg gtaggcttga taaccacttc ctccggcaaa
     2641 aaatattttt caggatggta aaatttatca agtgttgttt ttagccattc tttgtctatt
     2701 gcttcctttt cttgttgata taccttctcg atataggttt tcaactggcg tatctcttca
     2761 tttatatggg tacgcatttc ttcattgcag acagctttcg tttttacaca ttcgtcttta
     2821 tcatcccata gattgggatt gatggctaac tgagtaggag caacggagtc taactgcctt
     2881 ccgtttctaa aacggacata gattgtagcc atggattctg tatcatatcg tttggctgct
     2941 tttttaatga taaaggttac tttcatagac tttcaggttg aattttactc tgctgcaaat
     3001 ataaatattt tccccagcat tttccccaca tctgctaaat attttgcaat tcgattaaac
     3061 ttggattaaa atttaatagg attataacat attgaaatac agtgtaattg tggcgttttt
     3121 ccgcattttt ctttttaccc acattttccc cactttaatt atttcaaatg cggggtctgg
     3181 gtacaagaaa gaaagctaag tatttgatag ttcaatactt agctttttct tttgcttgaa
     3241 ttttccccac attttcccca cacgtgcaaa aaatatagca gtaagtcatt atttcttttg
     3301 gttgaacgta gagagtagcg atattaaaag aatccgatga gaaaagacaa atatttatct
     3361 atccattcag tttgatttct caggacttta catcgtcctg aaagtatttg ttggtaccga
     3421 ggacgcgtaa acatttacag ttgcatgtgg cctattgttt ttagccgtta aatattttat
     3481 aactattaaa tagcgataca aattgttcga aactaatatt gtttatatca tatattctcg
     3541 catgttttaa agctttatta aattgatttt ttgtaaacag tttttcgtac tctttgttaa
     3601 cccatttcat tacaaaagtt tcatattttt ttctctcttt aaatgccatt tttgctggct
     3661 ttctttttaa tacaattaat gtgctatcca ctttaggttt tggatggaaa taatacctag
     3721 gaatttttgc taatatagaa atatctacct ctgccattaa cagcaatgct agtgatctgt
     3781 ttgtatctaa taacatttta gcaaaaccat attccactat taaataactt attgtggctg
     3841 aactttcaaa aacaattttt cgaattatat ttgtgcttat gttgtaaggt atgctgccaa
     3901 atattttata tggattgtgg ctaggaaatg taaatttcag tatatcatca tttactattt
     3961 gatagttagg ataatttaag agcttattac gagttacctc acataattta gaatcaattt
     4021 ctatcgccgt tacaaaatta catctcttta ccaatccagc agtaaaatga cctttccctg
     4081 cacctatttc aaagatgtta tctttttcat ctaaacttat gcaattcatt attttttcta
     4141 tgtgatattt tgaagtaata aaattttgac tatcttttat atttactttg ttcattataa
     4201 cctctcctta atttattgca tctcttttcg aatatttatg ttttttgaga aaagaacgta
     4261 ctcatggttc atcccgatat gcgtatcggt ctgtatatca gcaactttct atgtgtttca
     4321 actacaatag tcatctattc tcatctttct gagtccaccc cctgcaaagc ccctctttac
     4381 gacataaaaa ttcggtcgga aaaggtatgc aaaagatgtt tctctcttta agagaaactc
     4441 ttcgggatgc aaaaatatga aaataactcc aattcaccaa attatatagc gactttttta
     4501 caaaatgcta aaatttgttg atttccgtca agcaattgtt gagcaaaaat gtcttttacg
     4561 ataaaatgat acctcaatat caactgttta gcaaaacgat atttctctta aagagagaaa
     4621 cacctttttg ttcaccaatc cccgactttt aatcccgcgg ccatgattga aaaaggaaga
     4681 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4741 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4801 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4861 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4921 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4981 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5041 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5101 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactagcc
     5161 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5221 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5281 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5341 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5401 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5461 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5521 cctcactgat taagcattgg taactgtcag accaagttta ctcataacgc gtcaattcga
     5581 gggggatcaa ttccgtgata ggtgggctgc ccttcctggt tggcttggtt tcatcagcca
     5641 tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc
     5701 cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg
     5761 ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac
     5821 gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg
     5881 ctataatgac cccgaagcag ggttatgcag cggaaaacgg aattgatccg gccacgatgc
     5941 gtccggcgta gaggatctga agatcagcag ttcaacctgt tgatagtacg tactaagctc
     6001 tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt ttaacgaact
     6061 aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc tcatgtttca
     6121 cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact taaatagcct
     6181 ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg
     6241 tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct tagagcctct
     6301 caaagcaatt ttgagtgaca caggaacact taacggctga catgggaatt cccctccacc
     6361 gcggtgggtt ccatcactgg aagataggca attagtagaa gcaatttgat tagtaatcta
     6421 aaagcactcc gataacgatt agagtgcttt tagattgttt atcattaatt atcaaagcaa
     6481 gtgcagttca ttatgccagg atgcgctcgc acagacgcca acccgtgaca ccgttgatgg
     6541 tcacgcggaa cagcaggctg ccgtccgggt taatcagacg ctcgtcaata atcttattgc
     6601 cattccacag ggtaccggtg acggtgatct ttttaccgtc aaaaacggca atgccttcat
     6661 acggacggcc gaaatagtca atcatattcg gggtcacacc atcaatcacc agcgtaccgt
     6721 aatgcaagat aaccttgaag tggtgatcgt caactgggta gacgacttta aagattttct
     6781 cgatttgacc catttggtca ccgctcaggc cttcgtacgg gatgatcaca tgaatatcaa
     6841 ttttcagacc gttctcgccg gacagaacga tgcgctgaat cggcgtcacg ctcacaccca
     6901 ggttctggaa cagagagctt acgccaccct gttccaggac ttgatccaaa ttataacccg
     6961 cggtctgacg ccaatcgcca acaaaatctt ccagagtaaa aaccatttta ttcttttacc
     7021 ttaattccgc aacactatta taaaatattt tttttaagta cctgagcaac aaaaagttgc
     7081 tcaggatttt gccatgtcag aggattcact taacttagtg ttgcaaaaaa taatcaaaca
     7141 aaacctcatc aggcatggca aaaatacaaa taaaatccga gaagctcact ccttagcacg
     7201 tggccagcat cttagcgtcg gtggttacaa agaaaattcg acaaactgtt atttttctat
     7261 ctatttattt gaattgtgag cggataacaa ttacctttgt cggcaattgt gagcggataa
     7321 caattaaata aagatattct cgtcaaacaa atataaataa tataaacatg gataagaaat
     7381 actcaatagg cttagctatc ggcacaaata gcgtcggatg ggcggtgatc actgatgaat
     7441 ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac agtatcaaaa
     7501 aaaatcttat aggggctcct ttatttgaca gtggagagac agcggaagcg actcgtctca
     7561 aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat ctacaggaga
     7621 ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt gaagagtctt
     7681 ttttggtgga agaagacaag aagcatgaac gtcatcctat ttttggaaat atagtagatg
     7741 aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa ttggtagatt
     7801 ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg attaagtttc
     7861 gtggtcattt tttgattgag ggagatttaa atcctgataa tagtgatgtg gacaaactat
     7921 ttatccagtt ggtacaaacc tacaatcaat tatttgaaga aaaccctatt aacgcaagtg
     7981 gagtagatgc taaagcgatt ctttctgcac gattgagtaa atcaagacga ttagaaaatc
     8041 tcattgctca gctccccggt gagaagaaaa atggcttatt tgggaatctc attgctttgt
     8101 cattgggttt gacccctaat tttaaatcaa attttgattt ggcagaagat gctaaattac
     8161 agctttcaaa agatacttac gatgatgatt tagataattt attggcgcaa attggagatc
     8221 aatatgctga tttgtttttg gcagctaaga atttatcaga tgctatttta ctttcagata
     8281 tcctaagagt aaatactgaa ataactaagg ctcccctatc agcttcaatg attaaacgct
     8341 acgatgaaca tcatcaagac ttgactcttt taaaagcttt agttcgacaa caacttccag
     8401 aaaagtataa agaaatcttt tttgatcaat caaaaaacgg atatgcaggt tatattgatg
     8461 ggggagctag ccaagaagaa ttttataaat ttatcaaacc aattttagaa aaaatggatg
     8521 gtactgagga attattggtg aaactaaatc gtgaagattt gctgcgcaag caacggacct
     8581 ttgacaacgg ctctattccc catcaaattc acttgggtga gctgcatgct attttgagaa
     8641 gacaagaaga cttttatcca tttttaaaag acaatcgtga gaagattgaa aaaatcttga
     8701 cttttcgaat tccttattat gttggtccat tggcgcgtgg caatagtcgt tttgcatgga
     8761 tgactcggaa gtctgaagaa acaattaccc catggaattt tgaagaagtt gtcgataaag
     8821 gtgcttcagc tcaatcattt attgaacgca tgacaaactt tgataaaaat cttccaaatg
     8881 aaaaagtact accaaaacat agtttgcttt atgagtattt tacggtttat aacgaattga
     8941 caaaggtcaa atatgttact gaaggaatgc gaaaaccagc atttctttca ggtgaacaga
     9001 agaaagccat tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa
     9061 aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca ggagttgaag
     9121 atagatttaa tgcttcatta ggtacctacc atgatttgct aaaaattatt aaagataaag
     9181 attttttgga taatgaagaa aatgaagata tcttagagga tattgtttta acattgacct
     9241 tatttgaaga tagggagatg attgaggaaa gacttaaaac atatgctcac ctctttgatg
     9301 ataaggtgat gaaacagctt aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa
     9361 aattgattaa tggtattagg gataagcaat ctggcaaaac aatattagat tttttgaaat
     9421 cagatggttt tgccaatcgc aattttatgc agctgatcca tgatgatagt ttgacattta
     9481 aagaagacat tcaaaaagca caagtgtctg gacaaggcga tagtttacat gaacatattg
     9541 caaatttagc tggtagccct gctattaaaa aaggtatttt acagactgta aaagttgttg
     9601 atgaattggt caaagtaatg gggcggcata agccagaaaa tatcgttatt gaaatggcac
     9661 gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg aaacgaatcg
     9721 aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc
     9781 aattgcaaaa tgaaaagctc tatctctatt atctccaaaa tggaagagac atgtatgtgg
     9841 accaagaatt agatattaat cgtttaagtg attatgatgt cgatgccatt gttccacaaa
     9901 gtttccttaa agacgattca atagacaata aggtcttaac gcgttctgat aaaaatcgtg
     9961 gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac tattggagac
    10021 aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg aaagctgaac
    10081 gtggtggttt gagtgaactt gataaagctg gttttatcaa acgccaattg gttgaaactc
    10141 gccaaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact aaatacgatg
    10201 aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa ttagtttctg
    10261 acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac catcatgccc
    10321 atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat ccaaaacttg
    10381 aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg attgctaagt
    10441 ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat atcatgaact
    10501 tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct ctaatcgaaa
    10561 ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc acagtgcgca
    10621 aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtacag acaggcgaat
    10681 tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag
    10741 actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat tcagtcctag
    10801 tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa gagttactag
    10861 ggatcacaat tatggaaaga agttcctttg aaaaaaatcc gattgacttt ttagaagcta
    10921 aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat agtctttttg
    10981 agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa aaaggaaatg
    11041 agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat tatgaaaagt
    11101 tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag cataagcatt
    11161 atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt ttagcagatg
    11221 ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca atacgtgaac
    11281 aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc gctgctttta
    11341 aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa gttttagatg
    11401 ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat ttgagtcagc
    11461 taggaggtga ctgaattaat gcggctgcaa tttttttggg cggggccgcc caaaaaaatc
    11521 ctagcaccct gcagcagtac tgcttgacca taagaacaaa aaaacttccg ataaagtttg
    11581 gaagataaag ctaaaagttc ttatctttgc agtctggaat gaattggcct atggttttag
    11641 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
    11701 agtcggtgct ttttttctag atgatgatta aggatcttgc gtacaatttg acgatcgttt
    11761 cacagcattt gtgcattggc tgcaaca
//
