LOCUS       sequence-477916-       14256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes sfGFP BbsI dropout for cloning in guide RNA sequence, 
            encodes mScarlet BsmBI/BsaI dropout for cloning in cargo sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14256)
  AUTHORS   VanDieren AJ, Barrick JE
  TITLE     UltraCAST: A Flexible All-In-One Suicide Vector for Modifying 
            Bacterial Genomes Using a CRISPR-Associated Transposon.
  JOURNAL   MicroPubl Biol. 2025 Aug 2;2025:10.17912/micropub.biology.001721. 
            doi: 10.17912/micropub.biology.001721. eCollection 2025.
  PUBMED    40827214
REFERENCE   2  (bases 1 to 14256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2025-08-2"; volume: 
            "2025:10.17912/micropub.biology.001721. doi"; pages: " 
            10.17912/micropub.biology.001721. eCollection 2025"
COMMENT     Sequence Label: ultra CAST
FEATURES             Location/Qualifiers
     source          1..14256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(549..1340,1341..1409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1172..1191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1410..1514)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1760..1779
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1988..2004
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2174..2190
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2406..2492
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2584..2611
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2878..2905)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(2921..2992)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             3715..3726
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(4307..4354)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     4350..4368
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(5803..5814)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(12776..13489)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     RBS             13496..13507
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(13587..13621)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     complement(13623..13640)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     oriT            13881..13989
                     /note="incP origin of transfer"
     rep_origin      join(14040..14256,1..140)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 aaaaaaaaga atatataagg cttttaaagc ttttaaggtt taacggttgt ggacaacaag
       61 ccagggatgt aacgcactga gaagccctta gagcctctca aagcaatttt gagtgacaca
      121 ggaacactta acggctgaca accggctggt aacaggatta gcagagcgag gtatgtaggc
      181 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
      241 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
      301 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
      361 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
      421 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
      481 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
      541 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
      601 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
      661 tctggcccca gtgctgcaat gataccgcga gagccacgct caccggctcc agatttatca
      721 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
      781 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
      841 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
      901 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
      961 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     1021 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     1081 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     1141 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     1201 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     1261 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     1321 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     1381 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     1441 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     1501 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     1561 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcctct cgcgcgtttc
     1621 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg acacggtcac agcttgtctg
     1681 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     1741 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     1801 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     1861 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     1921 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     1981 cgacgttgta aaacgacggc cagtgatcat acacatttcc ccgaaaagtg ccacctgacg
     2041 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     2101 ttagtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggtcc
     2161 cagtcacgac gttgtaaaac gacggccagt gaattcgagc tcggtacctg ttgatgcaac
     2221 cataaagtga tatttaataa ttatttataa tcagcaactt aaccacaaaa caaccatata
     2281 ttgatatctc acaaaacaac cataagttga tatttttgtg aatcgagtat ttcagcaaaa
     2341 ctactgcagt aaggactcta gaagggcacc aataactgcc ttaaaaaaag cagctatgcc
     2401 ggtcccaaat aaaatgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     2461 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
     2521 agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta
     2581 agcagaaggc catcctgacg gatggccttt ttgcgtggcc agtgccaagc ttgcatgcag
     2641 attgcagcat tacacgtctt gagcgattgt gtaggctgga gctgcttcgc gtaacatctc
     2701 tgtaactgct tgagacgtgg actgttgtga tatgccatat atgcagtggt gttcgccggc
     2761 gctggttccg gctgtcttgc ttagttgtga gtcgccaggc agtcatcggt atgatctgta
     2821 catgattcgt cagtgtgagc accactgacg agcagatttc cagctgagac ctataaacgc
     2881 agaaaggccc acccgaaggt gagccagtgt gactctagta gagagcgttc accgacaaac
     2941 aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg
     3001 agatccttat tatttataca gttcgtccat tccaccagtc gaatgacggc cttctgagcg
     3061 ctcatactgt tcgacaactg tgtaatcctc gttatgtgag gtgatgtcta acttacggtc
     3121 aacgttataa gctccaggca tttggacggg cttctttgct ttataagtgg tcttgaagtc
     3181 agctaaatag cgaccaccat ccttaagacg taacgccatc ttgatgtctc cttttaagac
     3241 accgtcctca gggtataaac gctctgtact agcctcccaa cccattgttt ttttctgcat
     3301 tacaggtccg tcagggggaa agttggtgcc gcgcagcttt accttgtaaa tcagggttcc
     3361 atcttctaat gaagtgtcct gggtcacagt cacagcgcca ccatcttcaa agttcatcac
     3421 gcgttcccat ttaaagcctt cggggaaaga ttgtttgtaa tagtctggga tgtcagcggg
     3481 atgtttgatg aacgcgcggc tgccgtacat aaactgcggg ctcagaatgt cccacgaaaa
     3541 tggcagaggt cctccttttg taacctttaa ctttgcagtt tgagtgccct cgtaggggcg
     3601 tccttcacct tcaccttcaa tctcgaactc atgtccgttc atggaacctt ccatatgcac
     3661 tttgaagcgc ataaactctt taattacagc ttctccttta ctgaccatct agtatttctc
     3721 ctctttctct agtagctaag atccgaacct gccgtttctt gagttgccgc gatgttaaga
     3781 aaacattcat aaattaaatg tgaattgccg cacacattat taaataagat ttacaaaatg
     3841 ttcaaaatga cgcatgaaat cacgtttcac tttcggtctc acgttgtatt gcgacgaatt
     3901 gccacgttgt cgcagtgtct gtactgtggg ttaatgtctg tgatcgccgt cgtgagttcg
     3961 tgtcatccgc gcatgattca gatggtcctg gagatcgttg cttgtaccgg ctgtttgtgg
     4021 ctctaccacg tctcatcagt ctagattctc attttcgcca aaagttggcc cagggcttcc
     4081 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaccac
     4141 tggccgtcga caggatcccc ggaaatgacc tcgaggaaaa tgtcgtaaac ttactgaagt
     4201 ttagaccatg aagaggcaat atcaatttat gggtgtgata attatcaatt tatgggtgta
     4261 attatcattt tatggttgta tcaacaacaa atgcctgagg tttcagcaaa aaacccctca
     4321 agacccgttt agaggcccca aggggttatg ctagttattg ctcagcggtg gcagcagcgc
     4381 agcagcctag gttattcgaa aaagttttta tttctacctg gtttactcac tctcggggtt
     4441 gaatgtctca aatagtcctc ccaaccaata tcttttggtg gcggtcgtaa catatcttct
     4501 gttacctcaa caccctcctc aaaaggattt ttccatgagt tgggtttatc gcccgatgtc
     4561 aacttcgatg caaaaacgaa atcttcttcg gttatcgttt catgctgatt atcaattgct
     4621 tcgatagatg cttgataaat aaggtttctc aacgaacgca tgtttccctg agagaatgca
     4681 tacaattttt tctgcaaact ttcgttggct aagccagcct gtttttcaaa aggtagggct
     4741 ttatcaaggt attccaaaaa agttttaaat acacctctac ctccctggta gctaaagggg
     4801 cgcagttcaa cctgaatgga aaatcgcccg tgaagttgcg agtttgcttg cagtacaact
     4861 tttgagtatg gcataccaaa tataacaatt ggacatttcg ttttgttaag tatcattttt
     4921 agccaattac ctacttgggt aagaacccga tttgaccttt cttccaccaa atgttggaac
     4981 tcatcgataa taatcagctt cacgccgacc gcagggatta attcagtcag tcttttcgtc
     5041 aatctagcta agtcagtttc ataaagtgct agcgggtcac ccatttcaac cagcaattcc
     5101 cttgctgcat ctactggctt cgcattgtct ggcaactcaa tatgcaaaac cggtattata
     5161 tcgcccccgg cttcggactc tcttctgttt tgatttaagt atttcttgat gacggtcgtt
     5221 ttacctacac ccgaagcacc atagaccatc atgcatgtag gctcagactc taggtctgat
     5281 agatctctac atctatccat gtaactcaag attttcctaa cgctcggtgt ggatacaaat
     5341 gcccttttag ctcttgatat acgagcctca cgcgtttcac tcatttgacg atttctcaga
     5401 cataatcaat atcccaatcg tcattatctg cactaacgat ttcatcttca tgtttttgta
     5461 tcgaggccgg gttcgcatta ctgattgact tagcccttgc gctattctct tgatgcctag
     5521 cgccacgcct ccgagctctg attttgttag tcttaacaat tgaacgatct gcgatttctt
     5581 caatttttat ttctgcatca atttccttgt cctcatcata ttctgctgaa tttagttctg
     5641 cttgatattt catgttatat ttatgttgcc aaagtgatac tctacttgca tattcgtagt
     5701 caatcgcatt aactgtaaag tactcattca tatcctcatc caacacccaa ataactgcca
     5761 tacactcagg gttatacttg aactgaactt tatggtttcc tttcttccct ctatattcag
     5821 ctaaacggtc attgctataa ctcagttctt tgtagacagt tatccctact ttagtaagtt
     5881 gtttgtaatc aacaatagca aatttaaagt ctaattcgtc tttagaacca gagaactcct
     5941 ctggttccca ttcttgacaa ccctttttcc acgcaacatt aggacaatta gtgcctctct
     6001 gattgggttt tttatgataa atatccacca accaaattaa gtaaatttct ctaatctcat
     6061 tgagtgtaag ggtagcttct cccacagagt cgtacccttc tctttgaagg tactggctga
     6121 aggatttccc aggtaaatcg tctaacagag aagtattaat agtcccgtag ttacgttcaa
     6181 catgaggttt gttgtcgggc gtttcaactt tattttgatg cacattgatc aatagtgatt
     6241 cacatgcttg atcaaatgct ttcgacaaaa actctttacc attatcagtt acgagtaggt
     6301 ctgggatgcc ataacatagc cattcattct cgatcgattc atacgaggag attaagtcat
     6361 ctttgcgttg tattgcattc ttaagtgcaa gggaaaccga cacatagcta ggaggctcga
     6421 aacctaaata aaaaccgatt acagctttac tgtaacaatc aaccaattga gtaagccaag
     6481 gtcggcccaa tgggattcga tactcttcgt gtactgcaaa aaggtcaacg acagtgtgat
     6541 ctatttcaac cctctctagc acgctagacg tgaggatctt ttttcccatt cggcgaaact
     6601 ctctcttagc tactctctcc cctttccctg cggctaataa ctcaaatggg gttttctttt
     6661 ttactcgctt tcttacagat tcgtacttag gatacgtgta tttcgttcca tgagttagat
     6721 tgtattgacg aacttttcgt cgaacacgtt tatatgcaga gctaacattg acttttcgtc
     6781 cagatataac tctttcaact gcctgttcca taatagaatc aacaactgtt gacacttttg
     6841 tctctctatt acctctatcc ttgatattag gtgctaagct aatggggttg tagtctgatt
     6901 ttcgaaaagc aagccaccat cgatatatcg ttattgcact gggtattttt ctactatagt
     6961 tttgctgtag ctctacacga tgtggttcaa ttgatttggc cacaatggtt tcgcactttc
     7021 gcgctaaaaa gcgaagaatg cttaatcgga aattaatctc taccgcaatt gtttcgggaa
     7081 atgctgatat atcttgatac gtaacacttt ctagacaatt agcgctagag acaagctcta
     7141 tcgattcaag cgtatcttgg gaagagactg cttttctatg gaaacttgag aaccctttct
     7201 tagccataac tggccaactc aaatcgagat tcaagagata gaggcttatc caaacgtgta
     7261 tcgagtaaac accttgacag caaatcgcac aaaattggta aaacagttcg gccttttaaa
     7321 cccaactgtt ctcccaagca tcttgcagat tgggcgccat tgtactttat tatttttaag
     7381 agagagttat gtacgttatt cagctcatct ttcgaagcat aacgatgcat gcgcttaaga
     7441 ttatttaata caaccgtatc cctaatatca ctctcttcaa caagctccaa ttctagccct
     7501 agttcagttg ctgcttttac tttcgcctcc cattcaacat caaaatcagg tttgttctta
     7561 gcataagctg actttacttc atatagaaca aactcgttgg tatcaaattg aactagaaag
     7621 tcaggaacat aggagtgaga ctgaccattg agataatata aaaatcgaat cggttgagcg
     7681 caaaagcgaa caatacttgg ctcaaaatca aagtgatagc aggcatcaca ctctagagta
     7741 gattctaccg taatcctctt actcatcttt acactgacat aacgatgaat gttttttccg
     7801 cgagatttcg ttagattgcg agcgggagta tggaatgcat actctaacgc cgaagtcgta
     7861 attgctgagg gcgtaggtaa acttgtcgcc atggtatatc tccttattaa aggtcagatt
     7921 aagggtacag gctgaaacga gttttctgaa tttgataagc catagctact aaaaatcgag
     7981 ttcccctcac ggggttgctc cgagagcatc ctgatattga gtcgaaagtt gcggttcgtc
     8041 tgtttgctgg actccccaag tgagtggtag tgcgctattg cagtatgctc tttttgagca
     8101 aaagatgatg ggtcaaattg ctcgcctcta gatagagcac gtttctccag gcgtctgatt
     8161 ttccttgcca gccctgctgc tgtgagctta tctatagatt gacagcgtct aaaggaaacg
     8221 tatgtacaat cttctgggac gagaacagtg ttggatatat gaaagtattg aagttgttcc
     8281 atttggacga aatagtgttg tttaagtagt aagtcgagtt ctatcttgtt cttgctgaca
     8341 aatgaaatct tttttccaac cgttgcatca caccaaagcg gaaatgaaac gccgatattt
     8401 cgtgtctcat actgatagtt aaatccatgc agaacgcgga gacactttgc agccagtgac
     8461 tcgttgttgc acaactctgg cagaaaggtg attgtcttat aataccattt cacagtcagt
     8521 ctcctttatg ctggaacatc ccacccttaa tcaggttagc cattaaaaag tgcatgtcgt
     8581 tgatagtttc ttgagcggga gttttgttgg cgctcaacac ttcaatatag tgctctgctt
     8641 gttgtaatat cgagaaaaaa tcttttccgg tagacggatg acggtagcaa gtgacatctt
     8701 cgcgatgaac cccaaaccgt ccgaccctta gtggctcagt ggcttcagga taccagtcgt
     8761 caatggttgc aatagctgct cccgttttaa aggcccctag tattggcgat cgttcaccat
     8821 caatagttgt actctgaaaa actcgactgt tttgagtttt agatttactt ttactgccac
     8881 tttctttttc tgtgaaaact tggcttggcc gcaccatagc attcgttggc aagtgcaagg
     8941 tggccctgac ttcaaatatc gccagcccat cagtactaga aaatgcggtt ttgatcattt
     9001 caactatagc agaccaattt ttattatttt taaagtcttg ccgtgaggtg tagttagtac
     9061 gaatatcttc aaatccaacc ttttccccgt tccaaggcca aggtgtaagt acaatgttcc
     9121 agcaataggc tttacgggta tttttccaca gccatttacc gttgcaaatg ttcatcaaat
     9181 atcgggttgc tagctcagtc cagccgattt tcgtttcgta gagctcgact aattgcacta
     9241 gcgtttgttt aactttgctt gagttacact tatatggttg acgtagttca gaagaaaacg
     9301 acacggagaa actgcattca atatgactcg caccgtatgg aacgtggcag aaatcaactt
     9361 gatgagggtt cccttgagct aacgcctcag cggtggcact ttttattggt tgacctgaga
     9421 catcataggc taatgatgcc gcttccattt gcccgagcag agtacgagaa ttgtaggtta
     9481 aaggtgtttt tctatcatcg ggccagacga caaaaaaaca gacatctgat gggtcgatag
     9541 agcgctcata ggctagattt gtcggtagtt tcatcgttga tacttcttgg ctgtaccact
     9601 aggtagtttt gatagttctt tagcgaccaa aagattgtgt tgggatcagt ctctgagcta
     9661 aaggtgattg agttaatgag tccaatgatg cactcggcga tagcgtgttt gtaacctctg
     9721 agggagtttg gtttatcttt gggctcttct aacaagtggt aaccgacgca gcttgccatg
     9781 agctgatggt cctctgtcat ggctgctaat agatcactta gattattgtt tgactgtgcg
     9841 taaagtgata accacctacc agcttctatt ggcagagatt cgatcgctgt ttctaatgtc
     9901 gagaatgagt tgatatgttt aaagtcatca atcgcaattt ttgctgaacc ccgagccaat
     9961 cgctttggta gtgatgtaac taacgtatct tggtcaatat gttgagcaaa gttggtgttc
    10021 aaaataaggc taaatacgac atcacactgc cagtcatccc gggtcgcggg agcggatata
    10081 gtcccgtcgc ctttttccac aaactctgct gtcaaacctc gcttctcgac atgtagttga
    10141 tggacgcata tcgcaaagga ttcaacacga aacgatgagg tggtgcgatt tatattccgt
    10201 tcaaatgcgt gaacgaagcc aaagaaagct gtaagtgatg gaatccccac cgttaccggg
    10261 gagcttaaag ctgttgcgcc acaaaccctg atattcggta ggactaaaaa cgatccatta
    10321 gtgtgttgct caccattatt gattgaattt gataactctc gtttcaaaag ctcagtaaag
    10381 agcttggtca tcgctggatc gtatgcaaaa cgttttgtgg ctcctaagta agataggtca
    10441 tgattcaaat gttggataag tatgttcgaa tcaagggtat ggtcttgcat tgcaaaccaa
    10501 gatctgacca tattttgaag ctcgattttg tattttctac ggagtgcaat ccgagagttt
    10561 tcaggtatga ggcttttgtt gaggcgctgg tactgtttta atgactgaac atgctctgac
    10621 gtcaaccaac ttcgtttact gtttaatcgg tgatgagggg ggctggaaaa cttagcgcca
    10681 gactttaaca ttctaaatgc gccgccacat gtcatcgcag tgacccccat gttggtagtt
    10741 cggctaaacc gcgttatcgc gctatgccga ttttcatctt ggagcctctg atgaaagtgg
    10801 ctttgcatcg atagcgatgc aacaggagag agtgaaatgt aggatgtatc actatcgggg
    10861 agagatattt gagtcaggta atttggtgcg agtgtgacat tgatagtcgt gagagtaata
    10921 tcagctagtt gttttgccat tgctttgcaa gttttttgag aacaacctaa ctttttcaaa
    10981 gtattccaaa gtgggtgatc tgcatcttta agaagctcac ccaaacatga gatctcacca
    11041 tcccaacaaa actcattggt gaggaatgcg cttttgttga cgtattttga atcatggcta
    11101 tatgaccaat ggtatggtgg gatttttgag gacgagagta ggaaactcgg taattcaccc
    11161 agggcttggg ttcgaatcac gccttcagaa cgtatatccg ggaattttgg gttatggctt
    11221 tgtctgtaat taacgcaatt tatgcagcta gcccaccatt tttcatctcg aagtttttgc
    11281 ttgcatttgg ctcgatcgag caggtctttc tgatcatcag tcttatcggt taaattgact
    11341 aatatcgtca gtgcgtcaag ttcattacca tcaattgcaa tatgcggtgt gagtggacga
    11401 aatgctctct taagttcagt tgttaagtcg tcaggattgg atgcgattag ttctttcaga
    11461 gtttgcattt accacctcga cacataaaac gaacggttaa actcatcgct ttggaactca
    11521 gctaaccaaa gacagaaaat atcgccgaaa tgaaatagat aatcggtaac atcaagaggg
    11581 ctgttgggct tgctttttcg atttggcttc aagatgcgtt gttcaaccat tgatgcaatc
    11641 tgatcgaggc tacaatttaa cattacagtc gcctctaacg cgttcatttt gaggttggcg
    11701 attaatccct tgtcttgcca taaacgattt tctaaatagc agagaagctc accaaggatg
    11761 atattgtgtt gaagattccg ctcaggtaac cgaattgaac cgaaaaacaa tcgaccaaga
    11821 agatctttta gtcgtaattc agacgtgctg acaacagcat gctcaaggtt gaactctact
    11881 tcatcttcga ttatcgagtg gaatgacctt ggccagtttg agaaaaattg aacgaacgaa
    11941 aagtgatcga actcgctatc tttaataccc atccaccaat gaactaaacc ccatcggtag
    12001 cttttaggaa gatttggcag aggtttagat tcatgaccag caagccagtt tgaaacagta
    12061 cacgctgcct catgaccgtt ctccctgctg gttaaggtga taggctcctt gcatttgcag
    12121 caaatgccct ttaacccaga tactcggtag tcaaactcct taccacagct acaagtggta
    12181 attaaaggta cgttatggct gtggcagtat tcgtacccct gaaagtgcca aaggtaggag
    12241 gcgtagccat tttctcgcag acacaaagga cagcagggga tggagtgcgt ccgtagtaaa
    12301 ctgcgaggaa agacttctgc acctctaaca accgcgctag ttgacggcga gtatttcatg
    12361 tttgttctgt taattgccaa cccaagtagc tctggcggtt cattaaatgt caattgtgca
    12421 agtttcagga atgacgcagt tcgtgcgctg gaactgtttt tagctgagta tgggtttatc
    12481 cgagttatat cagtcggaaa ggtttgatag ccattatggt caatgtcttg aaggaatcgt
    12541 ttagtggctt ctaggaagcg atggacatca ccgtagccat ttttgttagc cactcggata
    12601 aagaaacttt ctaaactttc atcgctgtaa ggtttaggtc tttgcaaaaa catatgtata
    12661 tctccttcgc tcgaattcgg atccagggat gtatatctcc ttcgctcgaa ttcggatcca
    12721 gggatgtata tctccttcgc tcgaattcgg atccataggt cttcatgcct ggtcatttgt
    12781 acagttcatc cataccatgc gtgatgcccg ctgcggttac gaactccagc agaaccatat
    12841 gatcgcgttt ctcgttcgga tctttagaca gaacgctttg cgtgctcaga tagtgattgt
    12901 ctggcagcag aacaggacca tcaccgattg gagtgttttg ctggtagtga tcagccagct
    12961 gcacgctgcc atcctccacg ttgtggcgaa ttttaaaatt cgctttaatg ccattttttt
    13021 gtttatcggc ggtgatgtaa acattgtggc tgttaaaatt gtattccagc ttatggccca
    13081 ggatattgcc gtcctcttta aagtcaatgc ctttcagctc aatgcggttt accagggtat
    13141 cgccttcaaa tttcacttcc gcacgcgttt tgtacgtgcc gtcatcctta aaggaaatcg
    13201 tgcgttcctg cacatagcct tccggcatgg cggacttgaa gaagtcatgc tgcttcatat
    13261 ggtccggata acgagcaaag cactgaacac cataagtcag cgtcgttacc agagtcggcc
    13321 aaggaaccgg cagtttacca gtagtacaga tgaacttcag cgtcagttta ccattagttg
    13381 cgtcaccttc accctcgcca cgcacggaaa acttatgacc gttgacatca ccatccagtt
    13441 ccaccagaat agggacgaca ccagtgaaca gctcttcgcc tttacgcatc tagtatttct
    13501 cctctttctc tagtagctaa gatccgaacc tgccgtttct tgagttgccg cgatggaaga
    13561 ctcgtttggt atatctccac gtcgacgcta gtattatacc taggactgag ctagctgtca
    13621 acctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    13681 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatttaa
    13741 ggtatacttt ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat
    13801 ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg
    13861 tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt
    13921 tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga
    13981 ggctggccga taagctctga taacgcagga aagaacatgg gcagttcaac ctgttgatag
    14041 tacgtactaa gctctcatgt ttcacgtact aagctctcaa gtttaacgta ctaagctctc
    14101 atgtttaacg aactaaaccc tcatggctaa cgtactaagc tctcatggct aacgtactaa
    14161 gctctcatgt ttcacgtact aagctctcat gtttgaacaa taaaattaat ataaatcagc
    14221 aacttaaata gcctctaagg ttttaagttt tataag
//
