LOCUS       sequence-477923-       10108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  7. BK1837- 
            pLV-CMV-SNCAintron1-AlphaSyn-d2EGFP-P2A-NLuc-SV40-Puro-WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Kantor B, Tagliafierro L, Gu J, Zamora ME, Ilich E, Grenier C, Huang
            ZY, Murphy S, Chiba-Falek O
  TITLE     Downregulation of SNCA Expression by Targeted Editing of DNA 
            Methylation: A Potential Strategy for Precision Therapy in PD.
  JOURNAL   Mol Ther. 2018 Nov 7;26(11):2638-2649. doi: 
            10.1016/j.ymthe.2018.08.019. Epub 2018 Aug 29.
  PUBMED    30266652
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymthe.2018.08.019"; journalName: "Mol Ther"; date: 
            "2018-11-7- 7"; volume: "26"; issue: "11"; pages: "2638-2649"
COMMENT     Sequence Label: pBK1837
FEATURES             Location/Qualifiers
     source          1..10108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        23..127
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(128..196,197..988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(346..365)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1159..1747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1648..1667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1901..1918
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2035..2056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2071..2076,2077..2094,2095..2101)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2109..2125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2114..2136
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2133..2149
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2133..2149
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2168..2188
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2170..2188
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2216..2442
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2443..2623
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2670..2795
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3288..3521
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3706..3750
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4017..4133
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4150..4453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4455..4732
                     /label=chicken beta-actin promoter
     regulatory      5703..5712
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5709..5711,5712..5714,5715..6425,6426..6431,
                     6432..6551)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(5754..5775)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6015..6034)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6362..6383
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6576..6632
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      6633..6642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6639..7154
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(7192..7212)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7197..7526
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7377..7512
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7536..8135
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7536..7555)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8032..8052
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8155..8743
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8208..8228)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8626..8637)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8842..9075
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9147..9268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9184..9203)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9238..9257
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9308..9443
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9463..9482)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9464..9482)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9492..9509)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9492..9508)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9501..9523)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9650..10105
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9737..9756)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9947..9968
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
       61 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      121 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      181 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      241 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      301 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      361 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      421 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      481 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
      541 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      601 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      661 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      721 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      781 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      841 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      901 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      961 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     1021 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     1081 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1141 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1201 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1261 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1321 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1381 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1441 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1501 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1561 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1621 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1681 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1741 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1801 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1861 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1921 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1981 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     2041 gagttagctc actcattagg caccccaggc tttccacttt atgcttccgg ctcgtatgtt
     2101 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     2161 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     2221 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     2281 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     2341 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     2401 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     2461 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     2521 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     2581 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     2641 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     2701 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     2761 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     2821 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     2881 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     2941 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     3001 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     3061 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     3121 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     3181 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     3241 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     3301 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     3361 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     3421 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     3481 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     3541 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     3601 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     3661 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     3721 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     3781 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     3841 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     3901 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     3961 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt
     4021 aaaagaaaag gggggattgg ggggtatagt gcaggggaaa gaatagtaga cataatagca
     4081 acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgatt
     4141 taattaacgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     4201 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     4261 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     4321 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     4381 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     4441 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
     4501 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
     4561 gggggggggg ggggggcgcg cgccrggsgg ggsggggsgg ggsgrggggs ggggcggggc
     4621 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
     4681 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgcgggcgtc
     4741 tttggaaatc ctggagaacg ccggatggga gacgaatggt cgtgggcacc gggagggggt
     4801 ggtgctgcca tgaggacccg ctgggccagg tctctgggag gtgagtactt gtccctttgg
     4861 ggagcctaag gaaagagact tgacctggct ttcgtcctgc ttctgatatt cccttctcca
     4921 caagggctga gagattaggc tgcttctccg ggatccgctt ttccccggga aacgcgagga
     4981 tgctccatgg agcgtgagca tccaactttt ctctcacata aaatctgtct gcccgctctc
     5041 ttggtttttc tctgtaaagt aagcaagctg cgtttggcaa ataatgaaat ggaagtgcaa
     5101 ggaggccaag tcaacaggtg gtaacgggtt aacaagtgct ggcgcggggt ccgctagggt
     5161 ggaggctgag aacgccccct cgggtggctg gcgcggggtt ggagacggcc cgcgagtgtg
     5221 agcggcgcct gctcagggta gatagctgag ggcggatgga tgtattcatg aaaggacttt
     5281 caaaggccaa ggagggagtt gtggctgctg ctgagaaaac caaacagggt gtggcagaag
     5341 cagcaggaaa gacaaaagag ggtgttctct atgtaggctc caaaaccaag gagggagtgg
     5401 tgcatggtgt ggcaacagtg gctgagaaga ccaaagagca agtgacaaat gttggaggag
     5461 cagtggtgac gggtgtgaca gcagtagccc agaagacagt ggagggagca gggagcattg
     5521 cagcagccac tggctttgtc aaaaaggacc agttgggcaa gaatgaagaa ggagccccac
     5581 aggaaggaat tctggaagat atgcctgtgg atcctgacaa tgaggcttat gaaatgcctt
     5641 ctgaggaagg gtatcaagac tacgaacctg aagaggagga gagtgactta attaaaccgg
     5701 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     5761 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     5821 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     5881 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     5941 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     6001 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     6061 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     6121 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     6181 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     6241 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     6301 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     6361 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     6421 acaagaagct tagccatggc ttcccgccgg aggtggagga gcaggatgat ggcacgctgc
     6481 ccatgtcttg tgcccaggag agcgggatgg accgtcaccc tgcagcctgt gcttctgcta
     6541 ggatcaatgt ggaggagggc ggctcgaggt cgagagccac gaacttctct ctgttaaagc
     6601 aagcaggaga cgtggaagaa aaccccggtc ccgccaccat ggtcttcaca ctcgaagatt
     6661 tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt gaacagggag
     6721 gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa aggattgtcc
     6781 tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat gaaggtctga
     6841 gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct gtggatgatc
     6901 atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt acgccgaaca
     6961 tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca
     7021 ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg atcaaccccg
     7081 acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg ctgtgcgaac
     7141 gcattctggc gtaagtcgac catgcatcta gacccagcgg cgcgcccctg tggaatgtgt
     7201 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7261 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7321 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     7381 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7441 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7501 tttttggagg cctaggcttt tgcaaaaagc ttaccatgac cgagtacaag cccacggtgc
     7561 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     7621 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     7681 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     7741 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     7801 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     7861 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     7921 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     7981 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     8041 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     8101 gcacctggtg catgacccgc aagcccggtg cctgacgggc gcgccgaatt cgacaatcaa
     8161 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     8221 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     8281 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8341 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8401 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8461 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8521 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     8581 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8641 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8701 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     8761 tgacttacaa ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     8821 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     8881 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8941 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9001 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9061 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     9121 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     9181 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9241 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     9301 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9361 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9421 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     9481 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     9541 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     9601 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     9661 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     9721 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9781 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     9841 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     9901 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     9961 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    10021 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    10081 aacaaaatat taacgcttac aatttagg
//
