LOCUS       sequence-477928-        8106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red 
            fluorescent fly eye marker to select for knock-in events, small fly 
            eye marker to select against backbone integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Bosch JA, Beltran PMJ, Cavers C, LaGraff JT, Melanson R, Singh A, 
            Chen W, Hu Y, Tattikota SG, Liu Y, Hashmi Y, Asara JM, Branon T, 
            Ting AY, Carr SA, Perrimon N
  TITLE     Multi-omic mapping of Drosophila protein secretomes reveals 
            tissue-specific origins and inter-organ trafficking
  JOURNAL   bioRxiv 2025.07.09.659702; doi: 
            https://doi.org/10.1101/2025.07.09.659702
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.07.09.659702; doi: https://doi.org/10.1101/2025.07.09.659702"
COMMENT     Sequence Label: pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)
FEATURES             Location/Qualifiers
     source          1..8106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             744..797
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             804..3449
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    3705..3738
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3964..3973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3970..4647
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(4100..4118)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(4150..4170)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4368..4387
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4562..4585
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    4768..4889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4805..4824)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4859..4878
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    4919..4952
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4988..5004)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4989..5005)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        5244..5482
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5429..5450
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     promoter        complement(5918..5936)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5957..5973)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5957..5973)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5970..5992)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5981..5997
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6005..6011,6012..6029,6030..6035))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6050..6071
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6188..6205)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6359..6947)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6439..6458)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7118..7909,7910..7978))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7741..7760
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7979..8083)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagcttgg
      661 gtacaagctt cacgacctga ggcgcgccgg gccagtaccc aaaaagcggg gggtccggtg
      721 gaagcggagg tagcgcggcc gccgagggcc gcggcagcct gctgacctgc ggcgatgtgg
      781 aggagaaccc cgggcccgct agcatgaagc tactgtcttc tatcgaacaa gcatgcgata
      841 tttgccgact taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga
      901 agaacaactg ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac
      961 atctgacaga agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc
     1021 ctcgagaaga ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt
     1081 taacaggatt atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt
     1141 cagtggagac tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat
     1201 cggaagagag tagtaacaaa ggtcaaagac agttgactgt atcgattgac tcggcagctc
     1261 atcatgataa ctccacaatt ccgttggatt ttatgcccag ggatgctctt catggatttg
     1321 attggtctga agaggatgac atgtcggatg gcttgccctt cctgaaaacg gaccccaaca
     1381 ataatgggtt ctttggcgac ggttctctct tatgtattct tcgatctatt ggctttaaac
     1441 cggaaaatta cacgaactct aacgttaaca ggctcccgac catgattacg gatagataca
     1501 cgttggcttc tagatccaca acatcccgtt tacttcaaag ttatctcaat aattttcacc
     1561 cctactgccc tatcgtgcac tcaccgacgc taatgatgtt gtataataac cagattgaaa
     1621 tcgcgtcgaa ggatcaatgg caaatccttt ttaactgcat attagccatt ggagcctggt
     1681 gtatagaggg ggaatctact gatatagatg ttttttacta tcaaaatgct aaatctcatt
     1741 tgacgagcaa ggtcttcgag tcaggttcca taattttggt gacagcccta catcttctgt
     1801 cgcgatatac acagtggagg cagaaaacaa atactagcta taattttcac agcttttcca
     1861 taagaatggc catatcattg ggcttgaata gggacctccc ctcgtccttc agtgatagca
     1921 gcattctgga acaaagacgc cgaatttggt ggtctgtcta ctcttgggag atccaattgt
     1981 ccctgcttta tggtcgatcc atccagcttt ctcagaatac aatctccttc ccttcttctg
     2041 tcgacgatgt gcagcgtacc acaacaggtc ccaccatata tcatggcatc attgaaacag
     2101 caaggctctt acaagttttc acaaaaatct atgaactaga caaaacagta actgcagaaa
     2161 aaagtcctat atgtgcaaaa aaatgcttga tgatttgtaa tgagattgag gaggtttcga
     2221 gacaggcacc aaagttttta caaatggata tttccaccac cgctctaacc aatttgttga
     2281 aggaacaccc ttggctatcc tttacaagat tcgaactgaa gtggaaacag ttgtctctta
     2341 tcatttatgt attaagagat tttttcacta attttaccca gaaaaagtca caactagaac
     2401 aggatcaaaa tgatcatcaa agttatgaag ttaaacgatg ctccatcatg ttaagcgatg
     2461 cagcacaaag aactgttatg tctgtaagta gctatatgga caatcataat gtcaccccat
     2521 attttgcctg gaattgttct tattacttgt tcaatgcagt cctagtaccc ataaagactc
     2581 tactctcaaa ctcaaaatcg aatgctgaga ataacgagac cgcacaatta ttacaacaaa
     2641 ttaacactgt tctgatgcta ttaaaaaaac tggccacttt taaaatccag acttgtgaaa
     2701 aatacattca agtactggaa gaggtatgtg cgccgtttct gttatcacag tgtgcaatcc
     2761 cattaccgca tatcagttat aacaatagta atggtagcgc cattaaaaat attgtcggtt
     2821 ctgcaactat cgcccaatac cctactcttc cggaggaaaa tgtcaacaat atcagtgtta
     2881 aatatgtttc tcctggctca gtagggcctt cacctgtgcc attgaaatca ggagcaagtt
     2941 tcagtgatct agtcaagctg ttatctaacc gtccaccctc tcgtaactct ccagtgacaa
     3001 taccaagaag cacaccttcg catcgctcag tcacgccttt tctagggcaa cagcaacagc
     3061 tgcaatcatt agtgccactg accccgtctg ctttgtttgg tggcgccaat tttaatcaaa
     3121 gtgggaatat tgctgatagc tcattgtcct tcactttcac taacagtagc aacggtccga
     3181 acctcataac aactcaaaca aattctcaag cgctttcaca accaattgcc tcctctaacg
     3241 ttcatgataa cttcatgaat aatgaaatca cggctagtaa aattgatgat ggtaataatt
     3301 caaaaccact gtcacctggt tggacggacc aaactgcgta taacgcgttt ggaatcacta
     3361 cagggatgtt taataccact acaatggatg atgtatataa ctatctattc gatgatgaag
     3421 ataccccacc aaacccaaaa aaagagtaag gtacctctag agtcgactaa agccaaatag
     3481 aaattattca gttctggctt aagtttttaa aagtgatatt atttatttgg ttgtaaccaa
     3541 ccaaaagaat gtaaataact aatacataat tatgttagtt ttaagttagc aacaaattga
     3601 ttttagctat attagctact tggttaataa atagaatata tttatttaaa gataattcgt
     3661 ttttattgtc agggagtgag tttgcttaaa aactcgtttg gatcataact tcgtataatg
     3721 tatgctatac gaagttatcg tacgggatct aattcaatta gagactaatt caattagagc
     3781 taattcaatt aggatccaag cttatcgatt tcgaaccctc gaccgccgga gtataaatag
     3841 aggcgcttcg tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa
     3901 gcgaaagcta agcaaataaa caagcgcagc tgaacaagct aaacaatcgg ctcgaagccg
     3961 gtcgccacca tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc
     4021 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     4081 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     4141 tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc
     4201 gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     4261 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctcca ggacggctcc
     4321 ttcatctaca aggtgaagtt catcggcgtg aacttcccct ccgacggccc cgtaatgcag
     4381 aagaagacta tgggctggga ggcgtccacc gagcgcctgt acccccgcga cggcgtgctg
     4441 aagggcgaga tccacaaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
     4501 aagtccatct acatggccaa gaagcccgtg cagctgcccg gctactacta cgtggactcc
     4561 aagctggaca tcacctccca caacgaggac tacaccatcg tggagcagta cgagcgcgcc
     4621 gagggccgcc accacctgtt cctgtagggg ccgcgactct agatcataat cagccatacc
     4681 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     4741 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4801 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4861 ggtttgtcca aactcatcaa tgtatcttaa ccgtctgtaa gcagcgaagg cccatacgat
     4921 aacttcgtat aatgtatgct atacgaagtt atgagctcca gcttttgttc cctttagtga
     4981 gggttatcga taccgtcgac ctcgaggggg ggcccggtac tcgcgagcga tcgcctcgag
     5041 cccagtggaa acccttgaaa tgcctttaag tcgagcccag tggaaaccct tgaaatgcct
     5101 ttaagtcgag cccagtggaa acccttgaaa tgcctttaag tcgagcccag tggaaaccct
     5161 tgaaatgcct ttaagtcgag cccagtggaa acccttgaaa tgcctttaag tcgactctag
     5221 gttaattaat cgcgaagcga gcgccggagt ataaatagag gcgcttcgtc tacggagcga
     5281 caattcaatt caaacaagca aagtgaacac gtcgctaagc gaaagctaag caaataaaca
     5341 agcgcagctg aacaagctaa acaatctgca gtaaagtgca agttaaagtg aatcaattaa
     5401 aagtaaccag caaccaagta aatcaactgc aactactgaa atctgccaag aagtaattat
     5461 tgaatacaag aagagaactc tgaataggga attgggaatt gagatctgtt ctagaaaaca
     5521 tcccataaaa catcccatat tcagccgcta gcagtaacat gactactact actacatagt
     5581 tatattcaag catatgtagt agtagtagtc atgttgcgaa atcaggcgag acatcggagt
     5641 tgaaactaaa actgaattac tagagtggac atatgcacct aggactagag caaactagtt
     5701 ctgatctgct agacaattgt tggcatcagg taggcatcac acacgattaa caacccctaa
     5761 aaatacactt tgaaaatatt gaaaatatgt ttttgtatac atttttgata ttttcaaata
     5821 atacgcagtt ataaaactca ttagctaacc cattttttct ttgcttatgc ttacagattg
     5881 caaagaacta gagccgcggg taccccagct tttgttccct ttagtgaggg ttaattgcgc
     5941 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6001 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6061 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6121 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6181 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6241 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6301 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6361 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6421 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6481 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6541 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6601 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6661 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6721 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6781 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6841 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6901 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6961 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7021 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7081 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7141 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7201 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7261 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7321 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7381 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7441 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7501 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7561 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7621 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7681 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7741 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7801 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7861 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7921 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7981 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8041 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8101 tgccac
//
