LOCUS       sequence-477930-       12383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac Vector expressing TEX264-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12383)
  AUTHORS   Hoyer MJ, Capitanio C, Smith IR, Paoli JC, Bieber A, Jiang Y, Paulo 
            JA, Gonzalez-Lozano MA, Baumeister W, Wilfling F, Schulman BA, 
            Harper JW
  TITLE     Combinatorial selective ER-phagy remodels the ER during 
            neurogenesis.
  JOURNAL   Nat Cell Biol. 2024 Mar;26(3):378-392. doi: 
            10.1038/s41556-024-01356-4. Epub 2024 Mar 1.
  PUBMED    38429475
REFERENCE   2  (bases 1 to 12383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-024-01356-4"; journalName: "Nat Cell Biol"; date: 
            "2024-03"; volume: "26"; issue: "3"; pages: "378-392"
COMMENT     Sequence Label: pAC150-TEX264-GFP
FEATURES             Location/Qualifiers
     source          1..12383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   58..92
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    426..459
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        609..988
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        989..1192
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1143..1163
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1189..1213
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2377..3372
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    3408..3542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3445..3464)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3499..3518
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3571..3604
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4246..5424
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4477..5415
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5372..5392
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5485..5509
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(6489..6491,6492..7205)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6489..6491,6492..7202)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6531..6552)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6792..6811)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(7208..7232)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7285..7326
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(7364..7381)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7370..7594
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8414..8476
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8514..8533)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8515..8533)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8540..8557)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8540..8556)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8549..8571)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8694..8996
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8875..8894
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             9345..10139
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9345..10139
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9399..9418)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10009..10028
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(10389..11180,11181..11249))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11012..11031
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11373..11961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11862..11881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12115..12132
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12249..12270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12285..12290,12291..12308,12309..12315)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12323..12339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12328..12350
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12347..12363
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12347..12363
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattaaccct cactaaaggg actagtcctg caggtttaaa cgaattcgcc cttttaaccc
       61 tagaaagata gtctgcgtaa aattgacgca tgcattcttg aaatattgct ctctctttct
      121 aaatagcgcg aatccgtcgc tgtgcattta ggacatctca gtcgccgctt ggagctcccg
      181 tgaggcgtgc ttgtcaatgc ggtaagtgtc actgattttg aactataacg accgcgtgag
      241 tcaaaatgac gcatgattat cttttacgtg acttttaaga tttaactcat acgataatta
      301 tattgttatt tcatgttcta cttacgtgat aacttattat atatatattt tcttgttata
      361 gatatcaact agaatgctag acctttcgtc ttcaagaatt ccgatcatat tcaataaccc
      421 ttaatataac ttcgtataat gtatgctata cgaagttatt aggtctgaag aggagtttac
      481 gtccagccaa gcttaggatc ccgggtaccg atggccgggc ctcgaggagc ttggcccatt
      541 gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc caacattacc
      601 gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      661 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      721 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      781 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      841 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      901 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      961 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     1021 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     1081 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     1141 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
     1201 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
     1261 ggaccgatcc agcctccccg gctagccgcc gccaccatga aaaagcctga actcaccgcg
     1321 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     1381 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     1441 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     1501 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     1561 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     1621 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     1681 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     1741 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     1801 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     1861 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     1921 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     1981 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     2041 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     2101 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     2161 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     2221 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtcgc gtctgcgttc
     2281 gaccaggctg cgcgttctcg cggccatagc aaccgacgta cggcgttgcg ccctcgccgg
     2341 cagcaagaag ccacggaagt ccgcccggag cagaaaatgc ccacgctact gcgggtttat
     2401 atagacggtc cccacgggat ggggaaaacc accaccacgc aactgctggt ggccctgggt
     2461 tcgcgcgacg atatcgtcta cgtacccgag ccgatgactt actggcgggt gctgggggct
     2521 tccgagacaa tcgcgaacat ctacaccaca caacaccgcc tcgaccaggg tgagatatcg
     2581 gccggggacg cggcggtggt aatgacaagc gcccagataa caatgggcat gccttatgcc
     2641 gtgaccgacg ccgttctggc tcctcatatc gggggggagg ctgggagctc acatgccccg
     2701 cccccggccc tcaccctcat cttcgaccgc catcccatcg ccgccctcct gtgctacccg
     2761 gccgcgcggt accttatggg cagcatgacc ccccaggccg tgctggcgtt cgtggccctc
     2821 atcccgccga ccttgcccgg caccaacatc gtgcttgggg cccttccgga ggacagacac
     2881 atcgaccgcc tggccaaacg ccagcgcccc ggcgagcggc tggacctggc tatgctggct
     2941 gcgattcgcc gcgtttacgg gctacttgcc aatacggtgc ggtatctgca gtgcggcggg
     3001 tcgtggcggg aggactgggg acagctttcg gggacggccg tgccgcccca gggtgccgag
     3061 ccccagagca acgcgggccc acgaccccat atcggggaca cgttatttac cctgtttcgg
     3121 gcccccgagt tgctggcccc caacggcgac ctgtataacg tgtttgcctg ggccttggac
     3181 gtcttggcca aacgcctccg ttccatgcac gtctttatcc tggattacga ccaatcgccc
     3241 gccggctgcc gggacgccct gctgcaactt acctccggga tggtccagac ccacgtcacc
     3301 acccccggct ccataccgac gatatgcgac ctggcgcgca cgtttgcccg ggagatgggg
     3361 gaggctaact gaagcttccc gggggtaccg aattcgtcga cagatctaac ttgtttattg
     3421 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3481 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     3541 tcatcaagct taggatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag
     3601 ttattaggtc cctcgaagag gttcactggc gcgttggatc cccgggtacc gagttgggag
     3661 ctcacgggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
     3721 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
     3781 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
     3841 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct
     3901 ttaggctgaa agagagattt agaatgacag tctagtggga gctcacgggg acagcccccc
     3961 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc
     4021 ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga
     4081 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc
     4141 tttgagcctg cagacacctg gggggatacg gggaaaaagc tttaggctga aagagagatt
     4201 tagaatgaca gaactcgatt tcattgcaga ctggcgcgcc cgtgaggctc cggtgcccgt
     4261 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     4321 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     4381 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     4441 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     4501 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     4561 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     4621 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     4681 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     4741 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     4801 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     4861 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     4921 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     4981 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     5041 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     5101 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     5161 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     5221 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     5281 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     5341 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     5401 tttcttccat ttcaggtgtc gtgaagaagc ttggtaccga gctcggatcc actagtccag
     5461 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctg cggtacatgt
     5521 cggacctgct actactgggc ctgattgggg gcctgactct cttactgctg ctgacgctgc
     5581 tggcctttgc cgggtactca gggctactgg ctggggtgga agtgagtgct gggtcacccc
     5641 ccatccgcaa cgtcactgtg gcctacaagt tccacatggg gctctatggt gagactgggc
     5701 ggcttttcac tgagagctgc agcatctctc ccaagctccg ctccatcgct gtctactatg
     5761 acaaccccca catggtgccc cctgataagt gccgatgtgc cgtgggcagc atcctgagtg
     5821 aaggtgagga atcgccctcc cctgagctca tcgacctcta ccagaaattt ggcttcaagg
     5881 tgttctcctt cccggcaccc agccatgtgg tgacagccac cttcccctac accaccattc
     5941 tgtccatctg gctggctacc cgccgtgtcc atcctgcctt ggacacctac atcaaggagc
     6001 ggaagctgtg tgcctatcct cggctggaga tctaccagga agaccagatc catttcatgt
     6061 gcccactggc acggcaggga gacttctatg tgcctgagat gaaggagaca gagtggaaat
     6121 ggcgggggct tgtggaggcc attgacaccc aggtggatgg cacaggagct gacacaatga
     6181 gtgacacgag ttctgtaagc ttggaagtga gccctggcag ccgggagact tcagctgcca
     6241 cactgtcacc tggggcgagc agccgtggct gggatgacgg tgacacccgc agcgagcaca
     6301 gctacagcga gtcaggtgcc agcggctcct cttttgagga gctggacttg gagggcgagg
     6361 ggcccttagg ggagtcacgg ctggaccctg ggactgagcc cctggggact accaagtggc
     6421 tctgggagcc cactgcccct gagaagggca aggagggagg ctcaggaggc tcaggaggct
     6481 cactcgaggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     6541 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     6601 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     6661 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     6721 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     6781 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     6841 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     6901 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     6961 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     7021 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     7081 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     7141 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     7201 agtagtgacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
     7261 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     7321 tctacgcgta ccggttaggg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     7381 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7441 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7501 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7561 agcaggcatg ctggggatgc ggtgggctct atggcggcgc gcctggatcc ccgggtaccg
     7621 agttgggagc tcacggggac agcccccccc caaagccccc agggatgtaa ttacgtccct
     7681 cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc
     7741 gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc
     7801 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg
     7861 gaaaaagctt taggctgaaa gagagattta gaatgacagt ctagtgggag ctcacgggga
     7921 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca
     7981 gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc
     8041 gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc
     8101 tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct ttaggctgaa
     8161 agagagattt agaatgacag aactcgattt cattgcagac tggccggcca ctagtacgcg
     8221 ccggctcgac atactagtta aaagttttgt tactttatag aagaaatttt gagtttttgt
     8281 ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt
     8341 aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca
     8401 gaccgataaa acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat
     8461 gattatcttt ctagggttaa aagggcgaat tcgcggccgc taaattcaat tcgccctata
     8521 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     8581 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     8641 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctatacgta cggcagttta
     8701 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     8761 ttattgacac gccggggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     8821 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     8881 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     8941 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     9001 caggcatgag attatcaaaa aggatcttca cctagatcct tttcacgtag aaagccagtc
     9061 cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa
     9121 acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact
     9181 gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg
     9241 ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca
     9301 ggggatcaag ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     9361 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     9421 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     9481 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     9541 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     9601 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     9661 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     9721 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     9781 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     9841 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     9901 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     9961 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
    10021 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
    10081 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa
    10141 ttattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
    10201 cacaccgcat caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    10261 ttctaaatac attcaaatat gtatccgctc atgagattat caaaaaggat cttcacctag
    10321 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10381 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10441 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10501 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10561 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10621 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10681 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10741 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10801 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10861 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10921 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10981 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    11041 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    11101 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11161 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11221 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11281 tatcagggtt attgtctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    11341 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    11401 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    11461 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    11521 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    11581 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    11641 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    11701 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    11761 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    11821 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    11881 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    11941 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    12001 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    12061 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    12121 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    12181 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    12241 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
    12301 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
    12361 gaccatgatt acgccaagct cag
//
