LOCUS       sequence-477946-       13529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CG6867-sfGFP under control of UAS promoter. Can be 
            used to generate transgenic fly lines by white+ fly eye selection 
            and phiC31 integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Bosch JA, Beltran PMJ, Cavers C, LaGraff JT, Melanson R, Singh A, 
            Chen W, Hu Y, Tattikota SG, Liu Y, Hashmi Y, Asara JM, Branon T, 
            Ting AY, Carr SA, Perrimon N
  TITLE     Multi-omic mapping of Drosophila protein secretomes reveals 
            tissue-specific origins and inter-organ trafficking
  JOURNAL   bioRxiv 2025.07.09.659702; doi: 
            https://doi.org/10.1101/2025.07.09.659702
REFERENCE   2  (bases 1 to 13529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.07.09.659702; doi: https://doi.org/10.1101/2025.07.09.659702"
COMMENT     Sequence Label: pUAS-CG6867-sfGFP
FEATURES             Location/Qualifiers
     source          1..13529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1453..2067,2138..2269,2473..2788,2850..3504,
                     3579..3852,4254..4325))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4777..4795
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    4918..4987
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    5225..5655
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5673..5706
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5719..5813
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5829..5862
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5875..5969
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5993..6231
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6178..6199
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    6277..6301
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9185..9209)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          10265..10330
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10460..10480
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10752..10873
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10789..10808)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10843..10862
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    10895..11325
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(11344..11364)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11344..11362)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11383..11399)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11383..11399)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11396..11418)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11407..11423
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11431..11437,11438..11455,11456..11461))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11476..11497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11614..11631)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11785..12373)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11865..11884)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12544..13335,13336..13404))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13167..13186
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13405..13509)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaaatgagct gatttaacaa
      421 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caagcttcac gacctaggcg cgccctagtt ccagtgaaat ccaagcattt tctaaattaa
      721 atgtattctt attattatag ttgttatttt tgatatatat aaacaacact attatgccca
      781 ccattttttt gagatgcatc tacacaagga acaaacactg gatgtcactt tcagttcaaa
      841 ttgtaacgct aatcactccg aacaggtcac aaaaaattac cttaaaaagt cataatatta
      901 aattagaata aatatagctg tgagggaaat atatacaaat atattggagc aaataaattg
      961 tacatacaaa tatttattac taatttctat tgagacgaaa tgaaccactc ggaaccattt
     1021 gagcgaaccg aatcgcgcgg aactaacgac agtcgctcca aggtcgtcga acaaaaggtg
     1081 aatgtgttgc ggagagcggg tgggagacag cgaaagagca actacgaaac gtggtgtggt
     1141 ggaggtgaat tatgaagagg gcgcgcgatt tgaaaagtat gtatataaaa aatatatccc
     1201 ggtgttttat gtagcgataa acgagttttt gatgtaaggt atgcaggtgt gtaagtcttt
     1261 tggttagaag acaaatccaa agtctacttg tggggatgtt cgaaggggaa atacttgtat
     1321 tctataggtc atatcttgtt tttattggca caaatataat tacattagct ttttgagggg
     1381 gcaataaaca gtaaacacga tggtaataat ggtaaaaaaa aaaaacaagc agttatttcg
     1441 gatatatgtc ggctactcct tgcgtcgggc ccgaagtctt agagccagat atgcgagcac
     1501 ccggaagctc acgatgagaa tggccagacc cacgtagtcc agcggcagat cggcggcgga
     1561 gaagttaagc gtctccagga tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt
     1621 gcagctaatt tcgcccggct ccacgtccgc ccattggtta atcagcagac cctcgttggc
     1681 gtaacggaac catgagaggt acgacaacca tttgaggtat actggcaccg agcccgagtt
     1741 caagaagaag ccgccaaaga gcaggaatgg tatgataacc ggcggaccca cagacagcgc
     1801 catcgaggtc gaggagctgg cgcaggatat tagatatccg aaggacgttg acacattggc
     1861 caccagagtg accagcgcca ggcagttgaa gaagtgcagc actccggccc gcagtccgat
     1921 catcggatag gcaatcgccg tgaagaccag tggcactgtg agaaaaagcg gcaattcggc
     1981 aatcgttttg cccagaaagt atgtgtcaca gcgataaagt cgacttcggg cctccctcat
     2041 aaaaactggc agctctgagg tgaacaccta aatcgaatcg attcattaga aagttagtaa
     2101 attattgaaa tgcaaatgta ttctaaacat gacttacatt tatcgtggca aagacgtttt
     2161 gaaaggtcat gttggtcagg aagaggaaga tggctccgtt gatattcatc acacccactt
     2221 gcgtgagttg ttggcccaaa aagatgaggc caatcaagat ggcaaccatc tgcaaattaa
     2281 aatgttactc gcatctcatt aatattcgcg agttaaatga aatttattta tcttctgcaa
     2341 aactataaac tatacatctc attgaaaaaa actaagaagg gtgtggaatc aggcaattct
     2401 atctaaaatc tagcgaattt gtttccaaga attgtaagcg ttatatcatt tgtttccact
     2461 ggaaccactc accgttgtct gaataagtcg cacttttacg aggagtggtt ccttgagcac
     2521 cgacagccag gatcgccaca ggaccgcccg gaactgcatg aaccaggtgg ccttgtaggt
     2581 gtacccattc tccggctgct ccagtggctt ctccagattt ttggtggcca acaactgctc
     2641 catatcccgg gctactttgc taatggcaaa attgtcgcat atcttggcga tccgatcacg
     2701 ggactcgatc tcccgtccgg gcacaacggc caacacctgt acgtaaaagt ccgccggatt
     2761 gtagttggta ggacactggg cacccacgct ggataggagt tgagatgtta tgtaatacta
     2821 gataccctta ataaacacat cgaactcact aggaaaagaa gtcgacggct tcgctgggag
     2881 tgcccaagaa agctaccctg ccctcggcca tcagaaggat cttgtcaaag agctcaaaca
     2941 gctcggaaga cggctgatga atggtcagga tgacggtctt gcccttctgc gacagcttct
     3001 tcagcacctg gacgacgctg tgggcggtaa aggagtccag tccggaggtg ggctcatcgc
     3061 agatcagaag cggcggatcg gttagagcct cggaggcgaa tgccagacgc ttcctttctc
     3121 cgccggacag acctttcacc ctgccgggca caccgatgat cgtgtgctga catttgctga
     3181 gcgaaagctc ctggatcacc tgatccacgc gggccactcg ctgccgatag gtcagatgtc
     3241 gtggcatccg caccatggct tggaaaatca ggtgttccct ggccgttagg gagccgataa
     3301 agaggtcatc ctgctggaca taggcgcacc tggcctgcat ctccttggcg tccacaggtt
     3361 ggccattgag cagtcgcatc ccggatggcg atacttggat gccctgcggc gatcgaaagg
     3421 caagggcatt cagcagggtc gtctttccgg caccggaact gcccatcacg gccaaaagtt
     3481 cgcccggata ggccacgccg caaactgagt ttcaaattgg taattggacc ctttattaag
     3541 atttcacaca gatcagccga ctgcgaatag aaactcaccg ttcttgagca aatgtttcct
     3601 gggcgccggt atgtgtcgct cgttgcagaa tagtccgcgt gtccggttga ccagctgccg
     3661 ccatccggag cccggctgat tgaccgcccc aaagatgtcc atattgtgcc aggcataggt
     3721 gaggttctcg gctagttggc cgctccctga accggagtcc tccggcggac tgggtggccg
     3781 gagcgtgccg tagtttttgg cctgcccgaa gccctggtta atgcagctct gcgaagccgc
     3841 tccgctgtca ccctgcaatg ataggggatc tcaaatatca actacaagcg ttatgctcat
     3901 ctaaccccga acaaaaagta ccccgaagta tcctacgaag taggtttata cttttattta
     3961 ttttttgtgc atctaggatc agcttaaaat atctggttgt tatatttttt gtaaaaaaga
     4021 atatagtcga aaatgaatgc ctttagatgt cttgatcatg atatgatctc aaaaattgtc
     4081 ttatatagcg agaacagcta ccagaataat ctgtttcgtg tcactatttg tttgtgcaat
     4141 tgcggtttgg gatttttgtg ggtcgcagtt ctcacgccgc agacaatttg atgttgcaat
     4201 cgcagttcct atagatcaag tgaacttaag atgtatgcac atgtactact cacattgttc
     4261 agatgctcgg cagatgggtg tttgctgcct ccgcgaatta atagctcctg atcctcttgg
     4321 cccattgccg ggatttttca cactttcccc tgcttaccca cccaaaacca atcaccaccc
     4381 caatcactca aaaaacaaac aaaaataaga agcgagagga gttttggcac agcactttgt
     4441 gtttaattga tggcgtaaac cgcttggagc ttcgtcacga aaccgctgac aaaatgcaac
     4501 tgaaggcgga cattgacgct acgtaacgct acaaacggtg gcgaaagaga tagcggacgc
     4561 agcggcgaaa gagacggcga tatttctgtg gacagagaag gaggcaaaca gcgctgactt
     4621 tgagtggaat gtcattttga gtgagaggta atcgaaagaa cctggtacat caaataccct
     4681 tggatcgaag taaatttaaa actgatcaga taagttcaat gatatccagt gcagtaaaaa
     4741 aaaaaaatgt tttttttatc tactttccgc aaaaatgggt tttattaact tacatacata
     4801 ctaggcgcgc cagatcgcaa gaagcttgat atcatcgatc tcgaggctgc atccaacgcg
     4861 ttgggagctc tccggatcaa ttcggcttca ggtaccgtcg acgatgtagg tcacggtctc
     4921 gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc
     4981 acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc
     5041 gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca cggtgagcac
     5101 gggacgtgcg acggcgtcgg ctggtgcgga tacgcggggc agcgtcagcg ggttctcgac
     5161 ggtcacggcg ggcatgtcga caagccgaat tgatccacta gaaggcctaa ttcggtacac
     5221 tagttggcca cgtaataagt gtgcgttgaa tttattcgca aaaacattgc atattttcgg
     5281 caaagtaaaa ttttgttgca taccttatca aaaaataagt gctgcatact ttttagagaa
     5341 accaaataat tttttattgc atacccgttt ttaataaaat acattgcata ccctctttta
     5401 ataaaaaata ttgcatactt tgacgaaaca aattttcgtt gcatacccaa taaaagatta
     5461 ttatattgca tacccgtttt taataaaata cattgcatac cctcttttaa taaagaatat
     5521 tgcatacgtt gacgaaacaa attttcgttg catacccaat aaaagattat tatattgcat
     5581 accttttctt gccataccat ttagccgatc aattgtgctc ggcaacagta tatttgtggt
     5641 gtgccaacca acaacactag tagtaccagc ttataacttc gtataatgta tgctatacga
     5701 agttatctgc aggcaggtcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     5761 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagac
     5821 tcccatggat aacttcgtat aatgtatgct atacgaagtt atggatccgc aggtcggagt
     5881 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     5941 gtcctccgag cggagtactg tcctccgagc ggagactcgt cgacagcgag cgccggagta
     6001 taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg
     6061 tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag
     6121 taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca
     6181 actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct gaatagggaa
     6241 ttgggaattc gcagatctgc ggccgcggct cgaatcacaa gtttgtacaa aaaagcaggc
     6301 tccgcggccg cccccttcac catgtttctg gcggttaacc agagtctaca gaataccagc
     6361 aagaagaagc gcaagcggcg gacggccgag gatgatccgc aggaggattc tggagaagga
     6421 acgccaccag ttcgtcccct ggaacccagt gcgtccagtg accagttggt cggagttggc
     6481 aatggagcca cttgccgggt gtgtaagtgc ccgggcaggc agtccttgac cctggtatac
     6541 atctacctag gcgccctcac cctcatcctg gccagcctct caatcgtctt ctacacgcac
     6601 acccaggacg tcgagtcctt gtcacaggtg gaagataact attcgtcgaa atcgttccgg
     6661 gtacaattcc aggcggagtt aatgcgctcg gaagaggaat taaggagact ggtcaacagg
     6721 ataatcgagg aggagcagga attggttggc agcaccaccg cgagtagcac cacccaatcg
     6781 agggatcctc tgaagaatga acgcaagttc acggataacc tgattagcca cacaacgcgt
     6841 cccacaggga aacgaatgcg ccgggacatc gcctcctccg ctccggcatc catgcatgct
     6901 gatccgctga tcgagttctt caatccgaac catcgcaagg ttttggagga gcaagacacc
     6961 gagatccgta agcgcacggg tcaaaagggc gccgctcccg gcggagatga gtggatctat
     7021 ctgaacacat actgccgtgt tccggagaag ataatcacgg gcttctgcaa gggtacccag
     7081 gactattgtc cgccggcacc gcaaggacca accggagaag tgggaccaaa gggaccaccg
     7141 ggaaacccgg gtctacctgg catccccggt ccaaagggaa atcgcggtga tgtgggatta
     7201 ccgggagccc ccggtgtgga tggcgtgggt catttgggtc ccgccggacc tcgcggtcct
     7261 aagggagatg ccggggccac cggaagagcc ggcttggatg gacgggatgg agtgcccggg
     7321 gagccgggac tcgatggcgt accgggtcgc gctggtgctg atggaaagaa tggcctgcct
     7381 ggacgcgatg gcaaggatgg gctacacggc aaggatggca aagatggtct gtccatcacg
     7441 ggacccaagg gtgcgcaggg accaccaggc gaacgaggtc tcaagggcat tgcgggtcca
     7501 cgtggtcgac ctggcaagcc gggaaccaat ggcatacccg gagtgcccgg catcaatgcc
     7561 tggaagctgc aatatcccaa tggcagctcc tccaacgatc tgctcatacc gccttctata
     7621 acagacatcc aagtgccgga cttccaacgc acggtgattg tggaggaggg aagatccctg
     7681 aatttgagct gcaccgccac gggaactccg acaccgcagg tggaatggcg acgtgaggat
     7741 ggtcgcacca ttaatgtcaa tggcgtagag atggcctcca tcagcggaca gttcctgaga
     7801 ttcaccaata tcacccgaca tcagatggcg gcctacacct gttttgccaa caatggcatc
     7861 gctcccgtgg caaatgccac ctacctcgtg gaagtgcaat ttgctcccat gatatcggtg
     7921 tatcgccaaa tgatctacgc ggaataccag agcagtgcca cactcgagtg tttggtggag
     7981 gcattccccg aagccattag gtattgggaa cgtgcctacg acggcaagat cctcgatccc
     8041 agcgacaagt acggcattga atcgtaccca gagggtttca agacaaccat gcgcttgacc
     8101 atcagtaatc tgcgtaagga tgactttggg tactatcatt gtgtggcccg aaatgaacta
     8161 aatgccacaa tggtcaactt tgaaatagca ccccaagatc cgaatagtga gaccccgtat
     8221 gtgggaaata acatgaaggt atacggtcaa agaccgccgg agagcgagtg tcccgtttgc
     8281 gatcagtgtc cagatcccag cttgtaccag tgcaaggact ccatactgaa taactttgaa
     8341 atccaggcca cgggcaatct cagctatccg ggattaccca agcgaccaaa gacttgctat
     8401 ctgtatgccg tgggcaagcc cgttttccac aaggtggtca atgaaaagtt cggctcctgg
     8461 cttagagatc catctccgga cagtgatcgt gagaagacgt tcgtcactaa cgaaaacgat
     8521 ccgtacaatc tgtttgagtt caccactcgc attcagtacc gaatgaatag catcccccgg
     8581 aggaaatacg agattcaaga aggctttcat ggcaatgcac atgtggtctt taacggttcg
     8641 ttttactatc aacaaagaaa ctcggatctg gtggtcaagc tggacttgac cagcctgaaa
     8701 aagataacca cacaattgcc atatgctgga gtggctgctg cgaataggct ttataccacg
     8761 gactacaact acatggactt caatgtggat gaagtgggcc tgtgggtgat ctatagcacc
     8821 tacaactcca acaatacgct ggtggccaag ttggatgcgg agacgttgaa gatgcagtac
     8881 aactttaata tcaccctgga ccatcatcag tttggtgaga tgttcatcgt gtgtggcaat
     8941 ctgtatgcca tcgattcggg aacggacaag aacacccaga ttcgatatgt agtggacttg
     9001 tacaagggca agctgctcaa cacgaatctg cccttctcga atccctttag ccataccacc
     9061 accgtgggct acaatcccct gactgtggaa ctctactcct gggacaaggg caacgcactc
     9121 acatatccca tacgctacaa tgagcagcgt ttaatctccg acaatagtaa gggtgggcgc
     9181 gccgacccag ctttcttgta caaagtggtg attcgagggt acctcggagg ctccggtgga
     9241 agcggaggta gcggcggatc cgtgtccaag ggcgaggagc tgttcaccgg cgtggtgccc
     9301 atcctggtgg agctggatgg cgacgtgaac ggccacaagt tcagcgtgcg cggcgagggc
     9361 gagggcgacg ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     9421 cccgtgccct ggcccaccct ggtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     9481 taccccgatc acatgaagca gcacgatttc ttcaagagcg ccatgcccga gggctacgtg
     9541 caggagcgca ccatcagctt caaggatgac ggcacctaca agacccgcgc cgaggtgaag
     9601 ttcgagggcg ataccctggt gaaccgcatc gagctgaagg gcatcgattt caaggaggat
     9661 ggcaacatcc tgggccacaa gctggagtac aacttcaaca gccacaacgt gtacatcacc
     9721 gccgataagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa tgtggaggat
     9781 ggctccgtgc agctggccga tcactaccag cagaacaccc ccatcggcga cggcccagtg
     9841 ctgctgcccg ataaccacta cctgagcacc cagagcgtgc tgtccaagga ccccaacgag
     9901 aagcgcgatc acatggtgct gctggagttc gtgaccgccg ccggcatcac cctgggcatg
     9961 gatgagctgt acaagtaggc aaactagttc tgatctgcta gacaattgtt ggcatcaggt
    10021 aggcatcaca cacgattaac aacccctaaa aatacacttt gaaaatattg aaaatatgtt
    10081 tttgtataca tttttgatat tttcaaataa tacgcagtta taaaactcat tagctaaccc
    10141 attttttctt tgcttatgct tacagattgc aaagaactag agccgcggga tctttgtgaa
    10201 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
    10261 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
    10321 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
    10381 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
    10441 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
    10501 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
    10561 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
    10621 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
    10681 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatggaa
    10741 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    10801 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    10861 tcaatgtatc ttatcatgtc tggttccaga gctctggcca cgtaataagt gtgcgttgaa
    10921 tttattcgca aaaacattgc atattttcgg caaagtaaaa ttttgttgca taccttatca
    10981 aaaaataagt gctgcatact ttttagagaa accaaataat tttttattgc atacccgttt
    11041 ttaataaaat acattgcata ccctctttta ataaaaaata ttgcatactt tgacgaaaca
    11101 aattttcgtt gcatacccaa taaaagatta ttatattgca tacccgtttt taataaaata
    11161 cattgcatac cctcttttaa taaagaatat tgcatacgtt gacgaaacaa attttcgttg
    11221 catacccaat aaaagattat tatattgcat accttttctt gccataccat ttagccgatc
    11281 aattgtgctc ggcaacagta tatttgtggt gtgccaacca acaacgagct ccagcttttg
    11341 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
    11401 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    11461 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    11521 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    11581 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    11641 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    11701 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    11761 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    11821 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11881 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11941 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    12001 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    12061 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    12121 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    12181 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    12241 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    12301 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    12361 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    12421 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    12481 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    12541 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    12601 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    12661 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    12721 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    12781 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    12841 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    12901 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    12961 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    13021 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    13081 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    13141 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    13201 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    13261 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    13321 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    13381 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    13441 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    13501 ggttccgcgc acatttcccc gaaaagtgc
//
