LOCUS       sequence-477949-       11747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ER-localized sfGFP-TurboID under control of UAS 
            promoter. Can be used to generate transgenic fly lines by white+ fly
            eye selection and phiC31 integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Bosch JA, Beltran PMJ, Cavers C, LaGraff JT, Melanson R, Singh A, 
            Chen W, Hu Y, Tattikota SG, Liu Y, Hashmi Y, Asara JM, Branon T, 
            Ting AY, Carr SA, Perrimon N
  TITLE     Multi-omic mapping of Drosophila protein secretomes reveals 
            tissue-specific origins and inter-organ trafficking
  JOURNAL   bioRxiv 2025.07.09.659702; doi: 
            https://doi.org/10.1101/2025.07.09.659702
REFERENCE   2  (bases 1 to 11747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.07.09.659702; doi: https://doi.org/10.1101/2025.07.09.659702"
COMMENT     Sequence Label: pUAS-sfGFP-TurboID-ER
FEATURES             Location/Qualifiers
     source          1..11747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1453..2067,2138..2269,2473..2788,2850..3504,
                     3579..3852,4254..4325))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4777..4795
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    4918..4987
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    5225..5655
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5673..5706
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5719..5813
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5829..5862
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5875..5969
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5993..6231
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6178..6199
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    6277..6301
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     sig_peptide     6322..6375
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             7162..8118
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(8152..8176)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          8483..8548
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8678..8698
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8970..9091
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9007..9026)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9061..9080
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9113..9543
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(9562..9582)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9562..9580)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9601..9617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9601..9617)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9614..9636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9625..9641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9649..9655,9656..9673,9674..9679))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9694..9715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9832..9849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10003..10591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10083..10102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10762..11553,11554..11622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11623..11727)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaaatgagct gatttaacaa
      421 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caagcttcac gacctaggcg cgccctagtt ccagtgaaat ccaagcattt tctaaattaa
      721 atgtattctt attattatag ttgttatttt tgatatatat aaacaacact attatgccca
      781 ccattttttt gagatgcatc tacacaagga acaaacactg gatgtcactt tcagttcaaa
      841 ttgtaacgct aatcactccg aacaggtcac aaaaaattac cttaaaaagt cataatatta
      901 aattagaata aatatagctg tgagggaaat atatacaaat atattggagc aaataaattg
      961 tacatacaaa tatttattac taatttctat tgagacgaaa tgaaccactc ggaaccattt
     1021 gagcgaaccg aatcgcgcgg aactaacgac agtcgctcca aggtcgtcga acaaaaggtg
     1081 aatgtgttgc ggagagcggg tgggagacag cgaaagagca actacgaaac gtggtgtggt
     1141 ggaggtgaat tatgaagagg gcgcgcgatt tgaaaagtat gtatataaaa aatatatccc
     1201 ggtgttttat gtagcgataa acgagttttt gatgtaaggt atgcaggtgt gtaagtcttt
     1261 tggttagaag acaaatccaa agtctacttg tggggatgtt cgaaggggaa atacttgtat
     1321 tctataggtc atatcttgtt tttattggca caaatataat tacattagct ttttgagggg
     1381 gcaataaaca gtaaacacga tggtaataat ggtaaaaaaa aaaaacaagc agttatttcg
     1441 gatatatgtc ggctactcct tgcgtcgggc ccgaagtctt agagccagat atgcgagcac
     1501 ccggaagctc acgatgagaa tggccagacc cacgtagtcc agcggcagat cggcggcgga
     1561 gaagttaagc gtctccagga tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt
     1621 gcagctaatt tcgcccggct ccacgtccgc ccattggtta atcagcagac cctcgttggc
     1681 gtaacggaac catgagaggt acgacaacca tttgaggtat actggcaccg agcccgagtt
     1741 caagaagaag ccgccaaaga gcaggaatgg tatgataacc ggcggaccca cagacagcgc
     1801 catcgaggtc gaggagctgg cgcaggatat tagatatccg aaggacgttg acacattggc
     1861 caccagagtg accagcgcca ggcagttgaa gaagtgcagc actccggccc gcagtccgat
     1921 catcggatag gcaatcgccg tgaagaccag tggcactgtg agaaaaagcg gcaattcggc
     1981 aatcgttttg cccagaaagt atgtgtcaca gcgataaagt cgacttcggg cctccctcat
     2041 aaaaactggc agctctgagg tgaacaccta aatcgaatcg attcattaga aagttagtaa
     2101 attattgaaa tgcaaatgta ttctaaacat gacttacatt tatcgtggca aagacgtttt
     2161 gaaaggtcat gttggtcagg aagaggaaga tggctccgtt gatattcatc acacccactt
     2221 gcgtgagttg ttggcccaaa aagatgaggc caatcaagat ggcaaccatc tgcaaattaa
     2281 aatgttactc gcatctcatt aatattcgcg agttaaatga aatttattta tcttctgcaa
     2341 aactataaac tatacatctc attgaaaaaa actaagaagg gtgtggaatc aggcaattct
     2401 atctaaaatc tagcgaattt gtttccaaga attgtaagcg ttatatcatt tgtttccact
     2461 ggaaccactc accgttgtct gaataagtcg cacttttacg aggagtggtt ccttgagcac
     2521 cgacagccag gatcgccaca ggaccgcccg gaactgcatg aaccaggtgg ccttgtaggt
     2581 gtacccattc tccggctgct ccagtggctt ctccagattt ttggtggcca acaactgctc
     2641 catatcccgg gctactttgc taatggcaaa attgtcgcat atcttggcga tccgatcacg
     2701 ggactcgatc tcccgtccgg gcacaacggc caacacctgt acgtaaaagt ccgccggatt
     2761 gtagttggta ggacactggg cacccacgct ggataggagt tgagatgtta tgtaatacta
     2821 gataccctta ataaacacat cgaactcact aggaaaagaa gtcgacggct tcgctgggag
     2881 tgcccaagaa agctaccctg ccctcggcca tcagaaggat cttgtcaaag agctcaaaca
     2941 gctcggaaga cggctgatga atggtcagga tgacggtctt gcccttctgc gacagcttct
     3001 tcagcacctg gacgacgctg tgggcggtaa aggagtccag tccggaggtg ggctcatcgc
     3061 agatcagaag cggcggatcg gttagagcct cggaggcgaa tgccagacgc ttcctttctc
     3121 cgccggacag acctttcacc ctgccgggca caccgatgat cgtgtgctga catttgctga
     3181 gcgaaagctc ctggatcacc tgatccacgc gggccactcg ctgccgatag gtcagatgtc
     3241 gtggcatccg caccatggct tggaaaatca ggtgttccct ggccgttagg gagccgataa
     3301 agaggtcatc ctgctggaca taggcgcacc tggcctgcat ctccttggcg tccacaggtt
     3361 ggccattgag cagtcgcatc ccggatggcg atacttggat gccctgcggc gatcgaaagg
     3421 caagggcatt cagcagggtc gtctttccgg caccggaact gcccatcacg gccaaaagtt
     3481 cgcccggata ggccacgccg caaactgagt ttcaaattgg taattggacc ctttattaag
     3541 atttcacaca gatcagccga ctgcgaatag aaactcaccg ttcttgagca aatgtttcct
     3601 gggcgccggt atgtgtcgct cgttgcagaa tagtccgcgt gtccggttga ccagctgccg
     3661 ccatccggag cccggctgat tgaccgcccc aaagatgtcc atattgtgcc aggcataggt
     3721 gaggttctcg gctagttggc cgctccctga accggagtcc tccggcggac tgggtggccg
     3781 gagcgtgccg tagtttttgg cctgcccgaa gccctggtta atgcagctct gcgaagccgc
     3841 tccgctgtca ccctgcaatg ataggggatc tcaaatatca actacaagcg ttatgctcat
     3901 ctaaccccga acaaaaagta ccccgaagta tcctacgaag taggtttata cttttattta
     3961 ttttttgtgc atctaggatc agcttaaaat atctggttgt tatatttttt gtaaaaaaga
     4021 atatagtcga aaatgaatgc ctttagatgt cttgatcatg atatgatctc aaaaattgtc
     4081 ttatatagcg agaacagcta ccagaataat ctgtttcgtg tcactatttg tttgtgcaat
     4141 tgcggtttgg gatttttgtg ggtcgcagtt ctcacgccgc agacaatttg atgttgcaat
     4201 cgcagttcct atagatcaag tgaacttaag atgtatgcac atgtactact cacattgttc
     4261 agatgctcgg cagatgggtg tttgctgcct ccgcgaatta atagctcctg atcctcttgg
     4321 cccattgccg ggatttttca cactttcccc tgcttaccca cccaaaacca atcaccaccc
     4381 caatcactca aaaaacaaac aaaaataaga agcgagagga gttttggcac agcactttgt
     4441 gtttaattga tggcgtaaac cgcttggagc ttcgtcacga aaccgctgac aaaatgcaac
     4501 tgaaggcgga cattgacgct acgtaacgct acaaacggtg gcgaaagaga tagcggacgc
     4561 agcggcgaaa gagacggcga tatttctgtg gacagagaag gaggcaaaca gcgctgactt
     4621 tgagtggaat gtcattttga gtgagaggta atcgaaagaa cctggtacat caaataccct
     4681 tggatcgaag taaatttaaa actgatcaga taagttcaat gatatccagt gcagtaaaaa
     4741 aaaaaaatgt tttttttatc tactttccgc aaaaatgggt tttattaact tacatacata
     4801 ctaggcgcgc cagatcgcaa gaagcttgat atcatcgatc tcgaggctgc atccaacgcg
     4861 ttgggagctc tccggatcaa ttcggcttca ggtaccgtcg acgatgtagg tcacggtctc
     4921 gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc
     4981 acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc
     5041 gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca cggtgagcac
     5101 gggacgtgcg acggcgtcgg ctggtgcgga tacgcggggc agcgtcagcg ggttctcgac
     5161 ggtcacggcg ggcatgtcga caagccgaat tgatccacta gaaggcctaa ttcggtacac
     5221 tagttggcca cgtaataagt gtgcgttgaa tttattcgca aaaacattgc atattttcgg
     5281 caaagtaaaa ttttgttgca taccttatca aaaaataagt gctgcatact ttttagagaa
     5341 accaaataat tttttattgc atacccgttt ttaataaaat acattgcata ccctctttta
     5401 ataaaaaata ttgcatactt tgacgaaaca aattttcgtt gcatacccaa taaaagatta
     5461 ttatattgca tacccgtttt taataaaata cattgcatac cctcttttaa taaagaatat
     5521 tgcatacgtt gacgaaacaa attttcgttg catacccaat aaaagattat tatattgcat
     5581 accttttctt gccataccat ttagccgatc aattgtgctc ggcaacagta tatttgtggt
     5641 gtgccaacca acaacactag tagtaccagc ttataacttc gtataatgta tgctatacga
     5701 agttatctgc aggcaggtcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     5761 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagac
     5821 tcccatggat aacttcgtat aatgtatgct atacgaagtt atggatccgc aggtcggagt
     5881 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     5941 gtcctccgag cggagtactg tcctccgagc ggagactcgt cgacagcgag cgccggagta
     6001 taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg
     6061 tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag
     6121 taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca
     6181 actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct gaatagggaa
     6241 ttgggaattc gcagatctgc ggccgcggct cgaatcacaa gtttgtacaa aaaagcaggc
     6301 tccgcggccg cccccttcac catgaagtta tgcatattac tggccgtcgt ggcctttgtt
     6361 ggcctctcgc tcggggaatc caagggcgag gagctgttca ccggcgtggt gcccatcctg
     6421 gtggagctgg atggcgacgt gaacggccac aagttcagcg tgcgcggcga gggcgagggc
     6481 gacgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     6541 ccctggccca ccctggtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     6601 gatcacatga agcagcacga tttcttcaag agcgccatgc ccgagggcta cgtgcaggag
     6661 cgcaccatca gcttcaagga tgacggcacc tacaagaccc gcgccgaggt gaagttcgag
     6721 ggcgataccc tggtgaaccg catcgagctg aagggcatcg atttcaagga ggatggcaac
     6781 atcctgggcc acaagctgga gtacaacttc aacagccaca acgtgtacat caccgccgat
     6841 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaatgtgga ggatggctcc
     6901 gtgcagctgg ccgatcacta ccagcagaac acccccatcg gcgacggccc agtgctgctg
     6961 cccgataacc actacctgag cacccagagc gtgctgtcca aggaccccaa cgagaagcgc
     7021 gatcacatgg tgctgctgga gttcgtgacc gccgccggca tcaccctggg catggatgag
     7081 ctgtacaaga ccggtggttc aggcggttct ggtggtagcg gcggtagcgg tggtagcggt
     7141 ggtagcggtg gcagcggcgg taaagacaat actgtgcctc tgaagctgat cgctctcctg
     7201 gctaatggcg agttccatag tggcgaacag ctgggagaaa ccctgggcat gtccagggcc
     7261 gctatcaaca agcacattca gactctgcgc gactggggcg tggacgtgtt caccgtgccc
     7321 ggaaagggct actctctgcc cgagcctatc ccgctgctga acgctaaaca gattctggga
     7381 cagctggacg gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa tcagtacctg
     7441 ctggatcgaa tcggcgagct gaagagtggg gatgcttgca ttgcagaata tcagcaggca
     7501 gggagaggaa gcagagggag gaaatggttc tctccttttg gagctaacct gtacctgagt
     7561 atgttttggc gcctgaagcg gggaccagca gcaatcggcc tgggcccggt catcggaatt
     7621 gtcatggcag aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa atggcccaat
     7681 gacctgtatc tgcaggatag aaagctggca ggcatcctgg tggagctggc cggaataaca
     7741 ggcgatgctg cacagatcgt cattggcgcc gggattaacg tggctatgag gcgcgtggag
     7801 gaaagcgtgg tcaatcaggg ctggatcaca ctgcaggaag cagggattaa cctggacagg
     7861 aatactctgg ccgctacgct gatccgagag ctgcgggcag ccctggaact gttcgagcag
     7921 gaaggcctgg ctccatatct gccacggtgg gagaagctgg ataacttcat caatagaccc
     7981 gtgaagctga tcattgggga caaagagatt ttcgggatta gccgggggat tgataaacag
     8041 ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg agaaatcagt
     8101 ctgcggtctg ccgaaaagaa ggacgagctg taatagaagg gtggggcgcc gacccagctt
     8161 tcttgtacaa agtggtgatt cgagggtacc tctagagcaa actagttctg atctgctaga
     8221 caattgttgg catcaggtag gcatcacaca cgattaacaa cccctaaaaa tacactttga
     8281 aaatattgaa aatatgtttt tgtatacatt tttgatattt tcaaataata cgcagttata
     8341 aaactcatta gctaacccat tttttctttg cttatgctta cagattgcaa agaactagag
     8401 ccgcgggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     8461 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     8521 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     8581 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     8641 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     8701 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     8761 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     8821 gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata atcagccata
     8881 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     8941 aacataaaat gaatggaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     9001 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9061 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gttccagagc tctggccacg
     9121 taataagtgt gcgttgaatt tattcgcaaa aacattgcat attttcggca aagtaaaatt
     9181 ttgttgcata ccttatcaaa aaataagtgc tgcatacttt ttagagaaac caaataattt
     9241 tttattgcat acccgttttt aataaaatac attgcatacc ctcttttaat aaaaaatatt
     9301 gcatactttg acgaaacaaa ttttcgttgc atacccaata aaagattatt atattgcata
     9361 cccgttttta ataaaataca ttgcataccc tcttttaata aagaatattg catacgttga
     9421 cgaaacaaat tttcgttgca tacccaataa aagattatta tattgcatac cttttcttgc
     9481 cataccattt agccgatcaa ttgtgctcgg caacagtata tttgtggtgt gccaaccaac
     9541 aacgagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     9601 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9661 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9721 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9781 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9841 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9901 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9961 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10021 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10081 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10141 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10201 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10261 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10321 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10381 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10441 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10501 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10561 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10621 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10681 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10741 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10801 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10861 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10921 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10981 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11041 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11101 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11161 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11221 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11281 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11341 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11401 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11461 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11521 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11581 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11641 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11701 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgc
//
