LOCUS       sequence-477959-       12301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination plasmid to generate C-terminal sfGFP fusions 
            under control of UAS promoter. Can be used to generate transgenic 
            fly lines by white+ fly eye selection and phiC31 integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12301)
  AUTHORS   Bosch JA, Beltran PMJ, Cavers C, LaGraff JT, Melanson R, Singh A, 
            Chen W, Hu Y, Tattikota SG, Liu Y, Hashmi Y, Asara JM, Branon T, 
            Ting AY, Carr SA, Perrimon N
  TITLE     Multi-omic mapping of Drosophila protein secretomes reveals 
            tissue-specific origins and inter-organ trafficking
  JOURNAL   bioRxiv 2025.07.09.659702; doi: 
            https://doi.org/10.1101/2025.07.09.659702
REFERENCE   2  (bases 1 to 12301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.07.09.659702; doi: https://doi.org/10.1101/2025.07.09.659702"
COMMENT     Sequence Label: pWal10-roe-sfGFP
FEATURES             Location/Qualifiers
     source          1..12301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1453..2067,2138..2269,2473..2788,2850..3504,
                     3579..3852,4254..4325))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4777..4795
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    4918..4987
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    5225..5655
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5673..5706
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5719..5813
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5829..5862
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5875..5969
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5993..6231
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6178..6199
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    6277..6401
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6426..6431,6432..6449,6450..6456)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6510..7169
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6576..6595)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7511..7816
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7695..7714
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7857..7981)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          9037..9102
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9232..9252
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9524..9645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9561..9580)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9615..9634
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9667..10097
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(10116..10136)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10116..10134)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10155..10171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10155..10171)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10168..10190)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10179..10195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10203..10209,10210..10227,10228..10233))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10248..10269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10386..10403)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10557..11145)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10637..10656)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11316..12107,12108..12176))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11939..11958
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12177..12281)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaaatgagct gatttaacaa
      421 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caagcttcac gacctaggcg cgccctagtt ccagtgaaat ccaagcattt tctaaattaa
      721 atgtattctt attattatag ttgttatttt tgatatatat aaacaacact attatgccca
      781 ccattttttt gagatgcatc tacacaagga acaaacactg gatgtcactt tcagttcaaa
      841 ttgtaacgct aatcactccg aacaggtcac aaaaaattac cttaaaaagt cataatatta
      901 aattagaata aatatagctg tgagggaaat atatacaaat atattggagc aaataaattg
      961 tacatacaaa tatttattac taatttctat tgagacgaaa tgaaccactc ggaaccattt
     1021 gagcgaaccg aatcgcgcgg aactaacgac agtcgctcca aggtcgtcga acaaaaggtg
     1081 aatgtgttgc ggagagcggg tgggagacag cgaaagagca actacgaaac gtggtgtggt
     1141 ggaggtgaat tatgaagagg gcgcgcgatt tgaaaagtat gtatataaaa aatatatccc
     1201 ggtgttttat gtagcgataa acgagttttt gatgtaaggt atgcaggtgt gtaagtcttt
     1261 tggttagaag acaaatccaa agtctacttg tggggatgtt cgaaggggaa atacttgtat
     1321 tctataggtc atatcttgtt tttattggca caaatataat tacattagct ttttgagggg
     1381 gcaataaaca gtaaacacga tggtaataat ggtaaaaaaa aaaaacaagc agttatttcg
     1441 gatatatgtc ggctactcct tgcgtcgggc ccgaagtctt agagccagat atgcgagcac
     1501 ccggaagctc acgatgagaa tggccagacc cacgtagtcc agcggcagat cggcggcgga
     1561 gaagttaagc gtctccagga tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt
     1621 gcagctaatt tcgcccggct ccacgtccgc ccattggtta atcagcagac cctcgttggc
     1681 gtaacggaac catgagaggt acgacaacca tttgaggtat actggcaccg agcccgagtt
     1741 caagaagaag ccgccaaaga gcaggaatgg tatgataacc ggcggaccca cagacagcgc
     1801 catcgaggtc gaggagctgg cgcaggatat tagatatccg aaggacgttg acacattggc
     1861 caccagagtg accagcgcca ggcagttgaa gaagtgcagc actccggccc gcagtccgat
     1921 catcggatag gcaatcgccg tgaagaccag tggcactgtg agaaaaagcg gcaattcggc
     1981 aatcgttttg cccagaaagt atgtgtcaca gcgataaagt cgacttcggg cctccctcat
     2041 aaaaactggc agctctgagg tgaacaccta aatcgaatcg attcattaga aagttagtaa
     2101 attattgaaa tgcaaatgta ttctaaacat gacttacatt tatcgtggca aagacgtttt
     2161 gaaaggtcat gttggtcagg aagaggaaga tggctccgtt gatattcatc acacccactt
     2221 gcgtgagttg ttggcccaaa aagatgaggc caatcaagat ggcaaccatc tgcaaattaa
     2281 aatgttactc gcatctcatt aatattcgcg agttaaatga aatttattta tcttctgcaa
     2341 aactataaac tatacatctc attgaaaaaa actaagaagg gtgtggaatc aggcaattct
     2401 atctaaaatc tagcgaattt gtttccaaga attgtaagcg ttatatcatt tgtttccact
     2461 ggaaccactc accgttgtct gaataagtcg cacttttacg aggagtggtt ccttgagcac
     2521 cgacagccag gatcgccaca ggaccgcccg gaactgcatg aaccaggtgg ccttgtaggt
     2581 gtacccattc tccggctgct ccagtggctt ctccagattt ttggtggcca acaactgctc
     2641 catatcccgg gctactttgc taatggcaaa attgtcgcat atcttggcga tccgatcacg
     2701 ggactcgatc tcccgtccgg gcacaacggc caacacctgt acgtaaaagt ccgccggatt
     2761 gtagttggta ggacactggg cacccacgct ggataggagt tgagatgtta tgtaatacta
     2821 gataccctta ataaacacat cgaactcact aggaaaagaa gtcgacggct tcgctgggag
     2881 tgcccaagaa agctaccctg ccctcggcca tcagaaggat cttgtcaaag agctcaaaca
     2941 gctcggaaga cggctgatga atggtcagga tgacggtctt gcccttctgc gacagcttct
     3001 tcagcacctg gacgacgctg tgggcggtaa aggagtccag tccggaggtg ggctcatcgc
     3061 agatcagaag cggcggatcg gttagagcct cggaggcgaa tgccagacgc ttcctttctc
     3121 cgccggacag acctttcacc ctgccgggca caccgatgat cgtgtgctga catttgctga
     3181 gcgaaagctc ctggatcacc tgatccacgc gggccactcg ctgccgatag gtcagatgtc
     3241 gtggcatccg caccatggct tggaaaatca ggtgttccct ggccgttagg gagccgataa
     3301 agaggtcatc ctgctggaca taggcgcacc tggcctgcat ctccttggcg tccacaggtt
     3361 ggccattgag cagtcgcatc ccggatggcg atacttggat gccctgcggc gatcgaaagg
     3421 caagggcatt cagcagggtc gtctttccgg caccggaact gcccatcacg gccaaaagtt
     3481 cgcccggata ggccacgccg caaactgagt ttcaaattgg taattggacc ctttattaag
     3541 atttcacaca gatcagccga ctgcgaatag aaactcaccg ttcttgagca aatgtttcct
     3601 gggcgccggt atgtgtcgct cgttgcagaa tagtccgcgt gtccggttga ccagctgccg
     3661 ccatccggag cccggctgat tgaccgcccc aaagatgtcc atattgtgcc aggcataggt
     3721 gaggttctcg gctagttggc cgctccctga accggagtcc tccggcggac tgggtggccg
     3781 gagcgtgccg tagtttttgg cctgcccgaa gccctggtta atgcagctct gcgaagccgc
     3841 tccgctgtca ccctgcaatg ataggggatc tcaaatatca actacaagcg ttatgctcat
     3901 ctaaccccga acaaaaagta ccccgaagta tcctacgaag taggtttata cttttattta
     3961 ttttttgtgc atctaggatc agcttaaaat atctggttgt tatatttttt gtaaaaaaga
     4021 atatagtcga aaatgaatgc ctttagatgt cttgatcatg atatgatctc aaaaattgtc
     4081 ttatatagcg agaacagcta ccagaataat ctgtttcgtg tcactatttg tttgtgcaat
     4141 tgcggtttgg gatttttgtg ggtcgcagtt ctcacgccgc agacaatttg atgttgcaat
     4201 cgcagttcct atagatcaag tgaacttaag atgtatgcac atgtactact cacattgttc
     4261 agatgctcgg cagatgggtg tttgctgcct ccgcgaatta atagctcctg atcctcttgg
     4321 cccattgccg ggatttttca cactttcccc tgcttaccca cccaaaacca atcaccaccc
     4381 caatcactca aaaaacaaac aaaaataaga agcgagagga gttttggcac agcactttgt
     4441 gtttaattga tggcgtaaac cgcttggagc ttcgtcacga aaccgctgac aaaatgcaac
     4501 tgaaggcgga cattgacgct acgtaacgct acaaacggtg gcgaaagaga tagcggacgc
     4561 agcggcgaaa gagacggcga tatttctgtg gacagagaag gaggcaaaca gcgctgactt
     4621 tgagtggaat gtcattttga gtgagaggta atcgaaagaa cctggtacat caaataccct
     4681 tggatcgaag taaatttaaa actgatcaga taagttcaat gatatccagt gcagtaaaaa
     4741 aaaaaaatgt tttttttatc tactttccgc aaaaatgggt tttattaact tacatacata
     4801 ctaggcgcgc cagatcgcaa gaagcttgat atcatcgatc tcgaggctgc atccaacgcg
     4861 ttgggagctc tccggatcaa ttcggcttca ggtaccgtcg acgatgtagg tcacggtctc
     4921 gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc
     4981 acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc
     5041 gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca cggtgagcac
     5101 gggacgtgcg acggcgtcgg ctggtgcgga tacgcggggc agcgtcagcg ggttctcgac
     5161 ggtcacggcg ggcatgtcga caagccgaat tgatccacta gaaggcctaa ttcggtacac
     5221 tagttggcca cgtaataagt gtgcgttgaa tttattcgca aaaacattgc atattttcgg
     5281 caaagtaaaa ttttgttgca taccttatca aaaaataagt gctgcatact ttttagagaa
     5341 accaaataat tttttattgc atacccgttt ttaataaaat acattgcata ccctctttta
     5401 ataaaaaata ttgcatactt tgacgaaaca aattttcgtt gcatacccaa taaaagatta
     5461 ttatattgca tacccgtttt taataaaata cattgcatac cctcttttaa taaagaatat
     5521 tgcatacgtt gacgaaacaa attttcgttg catacccaat aaaagattat tatattgcat
     5581 accttttctt gccataccat ttagccgatc aattgtgctc ggcaacagta tatttgtggt
     5641 gtgccaacca acaacactag tagtaccagc ttataacttc gtataatgta tgctatacga
     5701 agttatctgc aggcaggtcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     5761 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagac
     5821 tcccatggat aacttcgtat aatgtatgct atacgaagtt atggatccgc aggtcggagt
     5881 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     5941 gtcctccgag cggagtactg tcctccgagc ggagactcgt cgacagcgag cgccggagta
     6001 taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg
     6061 tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag
     6121 taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca
     6181 actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct gaatagggaa
     6241 ttgggaattc gcagatctgc ggccgcggct cgaatcacaa gtttgtacaa aaaagctgaa
     6301 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     6361 actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc
     6421 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt
     6481 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     6541 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     6601 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     6661 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     6721 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     6781 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     6841 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     6901 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     6961 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     7021 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     7081 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     7141 actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta aaagccagat
     7201 aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata
     7261 cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag
     7321 cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca
     7381 accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa
     7441 gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg
     7501 ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
     7561 tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc
     7621 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
     7681 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg
     7741 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
     7801 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata
     7861 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
     7921 ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg
     7981 tgattcgagg gtacctcgga ggctccggtg gaagcggagg tagcggcgga tccgtgtcca
     8041 agggcgagga gctgttcacc ggcgtggtgc ccatcctggt ggagctggat ggcgacgtga
     8101 acggccacaa gttcagcgtg cgcggcgagg gcgagggcga cgccaccaac ggcaagctga
     8161 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctggtgacca
     8221 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga tcacatgaag cagcacgatt
     8281 tcttcaagag cgccatgccc gagggctacg tgcaggagcg caccatcagc ttcaaggatg
     8341 acggcaccta caagacccgc gccgaggtga agttcgaggg cgataccctg gtgaaccgca
     8401 tcgagctgaa gggcatcgat ttcaaggagg atggcaacat cctgggccac aagctggagt
     8461 acaacttcaa cagccacaac gtgtacatca ccgccgataa gcagaagaac ggcatcaagg
     8521 ccaacttcaa gatccgccac aatgtggagg atggctccgt gcagctggcc gatcactacc
     8581 agcagaacac ccccatcggc gacggcccag tgctgctgcc cgataaccac tacctgagca
     8641 cccagagcgt gctgtccaag gaccccaacg agaagcgcga tcacatggtg ctgctggagt
     8701 tcgtgaccgc cgccggcatc accctgggca tggatgagct gtacaagtag gcaaactagt
     8761 tctgatctgc tagacaattg ttggcatcag gtaggcatca cacacgatta acaaccccta
     8821 aaaatacact ttgaaaatat tgaaaatatg tttttgtata catttttgat attttcaaat
     8881 aatacgcagt tataaaactc attagctaac ccattttttc tttgcttatg cttacagatt
     8941 gcaaagaact agagccgcgg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     9001 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     9061 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     9121 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     9181 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
     9241 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
     9301 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
     9361 gcactgctat acaagaaaat tatggaaaaa tatttgatgt atagtgcctt gactagagat
     9421 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     9481 ccccctgaac ctgaaacata aaatgaatgg aattgttgtt gttaacttgt ttattgcagc
     9541 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9601 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggttcca
     9661 gagctctggc cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc
     9721 ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag
     9781 aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt
     9841 taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat
     9901 tattatattg catacccgtt tttaataaaa tacattgcat accctctttt aataaagaat
     9961 attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
    10021 ataccttttc ttgccatacc atttagccga tcaattgtgc tcggcaacag tatatttgtg
    10081 gtgtgccaac caacaacgag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
    10141 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    10201 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    10261 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    10321 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    10381 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10441 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10501 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10561 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10621 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10681 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10741 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10801 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10861 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10921 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10981 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    11041 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    11101 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    11161 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    11221 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    11281 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    11341 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    11401 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    11461 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11521 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11581 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    11641 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11701 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11761 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    11821 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11881 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    11941 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    12001 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    12061 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    12121 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    12181 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    12241 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    12301 c
//
