LOCUS       sequence-47798-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of mouse Alpha6 (Chrna6) with internal mEGFP 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Xiao C, Srinivasan R, Drenan RM, Mackey ED, McIntosh JM, Lester HA
  TITLE     Characterizing functional alpha6beta2 nicotinic acetylcholine 
            receptors in vitro: mutant beta2 subunits improve membrane 
            expression, and fluorescent proteins reveal responsive cells.
  JOURNAL   Biochem Pharmacol. 2011 Oct 15;82(8):852-61. Epub 2011 May 17.
  PUBMED    21609715
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Pharmacol."; date: "2011-10-15"; volume: "82(8)"; pages: "852-61. 
            Epub 2011 May 17"
COMMENT     Sequence Label: pcDNA-zeo: alpha6mGFP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(323..340)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    329..553
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(686..705)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgtcaggcag gagcacctgg aaagagaaga tcaagccagc agcctgcacg gtgggtggca
       61 gagaattcag agcactcgtc cgatgttgaa gatgtcatcg agagtgttca attcatagca
      121 gaaaacatga agagccacaa tgaaacaaac gaggtagaag acgactggaa atacatggct
      181 atggtggtgg acagagtctt cctttgggta tttataattg tctgtgtgtt tggaactgtg
      241 gggctatttc tgcagccact gcttgggaac acaggaaagt cttaactcga gtctagaggg
      301 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
      361 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
      421 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
      481 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
      541 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
      601 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      661 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      721 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      781 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcccg tagtgggcca
      841 tccccctgat
//
