LOCUS       sequence-47799-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Blount BA, Weenink T, Vasylechko S, Ellis T
  TITLE     Rational diversification of a promoter providing fine-tuned 
            expression and orthogonal regulation for synthetic biology.
  JOURNAL   PLoS One. 2012;7(3):e33279. Epub 2012 Mar 19.
  PUBMED    22442681
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33279. Epub 2012 Mar 19"
COMMENT     Sequence Label: pTAL5-BB
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..37
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             38..64
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 aatggcttcc tcccctccaa agaaaaagag aaaggttagt tggaaggacg caagtggttg
       61 gtctagaatg catgcggatc ccattcgtcc gcgcaggcca agtcctgccc gcgagcttct
      121 gcccggaccc caaccggata gggttcagcc gactgcagat cgtggggtgt ctgcgcctgc
      181 tggcagccct ctggatggct tgcccgctcg gcggacggtg tcccggaccc ggctgccatc
      241 tccccctgcg ccctcacctg cgttctcggc gggcagcttc agcgatctgc tccgtccgtt
      301 cgatccgtcg cttcttgata catcgcttct tgattcgatg cctgccgtcg gcacgccgca
      361 tacagcggct gccccagcag agtgggatga ggcgcaatcg gctctgcgtg cagccgatga
      421 cccgccaccc accgtgcgtg tcgctgtcac tgccgcgcgg ccgccgcgcg ccaagccggc
      481 cccgcgacgg cgtgctgcgc aaccctccga cgcttcgccg gccgcgcagg tggatctacg
      541 cacgctcggc tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt
      601 ggcgcagcac cacgaggcac tggtgggcna tgggtttaca cacgcgcaca tcgttgcgct
      661 cagccaacac ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc
      721 gttgccagag gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg
      781 cgccctggag gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga
      841 cacaggccaa cttgt
//
