LOCUS       sequence-47800-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Kowalczyk SW, Kapinos L, Blosser TR, Magalhaes T, van Nies P, Lim 
            RY, Dekker C
  TITLE     Single-molecule transport across an individual biomimetic nuclear 
            pore complex.
  JOURNAL   Nat Nanotechnol. 2011 Jun 19;6(7):433-8. doi: 10.1038/nnano.2011.88.
  PUBMED    21685911
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nnano.2011"; journalName: "Nat Nanotechnol"; date: 
            "2011-06-19- 19"; volume: "6"; issue: "7"; pages: "433-8"
COMMENT     Sequence Label: pPEP-TEV cNup98 (1-498)
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             164..184
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(262..280)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      276..323
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(498..503,504..520,521..526))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(604..621)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(855..874)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttaagaagg agatatacca tgggccacca tcaccatcat cacatgcaaa aatacttaga
       61 agataaagct caagaattgg tgcgactgga aggagaggtt cgctccctcc ttaaggacat
      121 aagtgagaaa gttgcggttt acagcaccgg cggtggtggc ggtgagaatt tatattttca
      181 agggggatcc cgggtcgact cgagaattct aagatccggc tgctaacaaa gcccgaaagg
      241 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      301 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat cccgcaagag
      361 gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag
      421 gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa
      481 ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattact
      541 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      601 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      661 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      721 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      781 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      841 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      901 tgttccgacc ctgccgctta ccggatacct gtccgc
//
