LOCUS       sequence-478128-        6750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses FecB of M. marinum in mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Lissner R, Franklin A, Benedict ST, Charitou V, Speer A, Knol J, 
            Jimenez C, Moynihan PJ, Nejentsev S, Kuijl C, Bitter W
  TITLE     A new role for lipoproteins LpqZ and FecB in orchestrating 
            mycobacterial cell envelope biogenesis.
  JOURNAL   mBio. 2025 Dec 12:e0211925. doi: 10.1128/mbio.02119-25.
  PUBMED    41384969
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2025 
            Dec 12:e0211925. doi: 10.1128/mbio.02119-25."
COMMENT     Sequence Label: pSMT3-fecB
FEATURES             Location/Qualifiers
     source          1..6750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1541..1557)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1541..1557)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1554..1576)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1565..1581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1589..1595,1596..1613,1614..1619))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1634..1655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1772..1789)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1943..2531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2023..2042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6730..6746
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6731..6747
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttccgat gccggtgttg gcgccggtga ccacaacgac gcgcccgctt tgatcggggg
       61 acgtctgcgg ccgaccattt acgggtcttg ttgtcgttgg cggtcatggg ccgaacatac
      121 tcacccggat cggagggccg aggtcacggt cgaacgaggg gcatgacccg gtgcggggct
      181 tcttgcactc ggcataggcg agtgctaaga ataacgttgg cactcgcgac cggtgagtgc
      241 taggtcggga cggtgaggcc aggcccgtcg tcgctgcgag tggcagcgag gacaacttga
      301 gccgtccgtc gcgggcactg cgcccggcca gcgtaagtag cggggttgcc gtcacccggt
      361 gacccccgtt tcatccccga tccggaggaa tcacgctagc gtgcaattcg gccggatgcg
      421 actgggtctg gtcgcggtat ctctgagtgt cgcaatcgtg atgtgctccg gttgcgggtc
      481 aaaccaaccc gccaccaaag cgtcgcgttc ggtgaccact cctaccaccc agatcgcggg
      541 cgccggggtg ctgggcaacg accgcaaggc cgatgagtcg tgcgcgcgcg aggcggccgc
      601 acccgacccg gggccggcaa cccgcccggc gcacaacgcc gcgggcgtca ccccggatgt
      661 cgcccagatc tccgccgaac cgcagcgcat cgtggtgctc tccggcgacc agctcgacgc
      721 gctgtgtgcg ctgggactgc aatcccgggt ggtcggtgcc gccttcccgg acggttccaa
      781 tagccagccc tcctacctcg gcaccgccgt gcacgacctg ccgggggtcg gcagccggac
      841 cgcaccggat ctgaaggcga tcgatgcggc ccacccggat ctgatcctgg gctcagaagc
      901 gctgacaccg aatttgtatg cgcagttggc ggcgatcgcc cccaccgtgt tcacggcggc
      961 accgggggcg gcgtgggagg acaatctgcg cggcgtcggg gccgcgaccg cccgcagcgc
     1021 cgcggtcgag gagctgctca gcggattcac ccagcgagcc agacaggtgg ggaccaaaca
     1081 tgacgcaacc catttccaag cgtcgatcgt ccagctgacc cgcgacagca tgcggatcta
     1141 tgggtcccgc aactttgcgg ccagtgtgct cgccgcggtc ggggtggatc ggcccgcgtc
     1201 gcagcggttc accgacaagc cttatctcga gatgggcacc accgacgccg aacttgccaa
     1261 gggaccggac ttttcggccg ctgacgccga catcatctac gtgtcctgcg cctcccccgc
     1321 ggccgccgaa cgagccgcta ccgtgctgga cagcggtccg tggcgcaagc tgtccgccaa
     1381 ccacgacaac cgggtcctga tcgtcaacga cgagatatgg cagacgggtg agggtttagt
     1441 cgccgctcgg ggcattgtcg atgacctgcg ctgggtgaac gcaccgatca actgatctag
     1501 agcggccgcc accgcggtgc gagccagatc tggtaatcat gtcatagctg tttcctgtgt
     1561 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     1621 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     1681 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     1741 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1801 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1861 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1921 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1981 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2041 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2101 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2161 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2221 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2281 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2341 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2401 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2461 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2521 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2581 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2641 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2701 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2761 tagttgcctg actccccgac gtggccgacc agcccgtcat cgtcaacgcc tgatccgcgg
     2821 tgcggacagg ccgtgtcgcg accggccgtg cggaattaag ccggcccgta ccctgtgaat
     2881 agaggtccgc tgtgacacaa gaatccctgt tacttctcga ccgtattgat tcggatgatt
     2941 cctacgcgag cctgcggaac gaccaggaat tctgggagcc gctggcccgc cgagccctgg
     3001 aggagctcgg gctgccggtg ccgccggtgc tgcgggtgcc cggcgagagc accaaccccg
     3061 tactggtcgg cgagcccggc ccggtgatca agctgttcgg cgagcactgg tgcggtccgg
     3121 agagcctcgc gtcggagtcg gaggcgtacg cggtcctggc ggacgccccg gtgccggtgc
     3181 cccgcctcct cggccgcggc gagctgcggc ccggcaccgg agcctggccg tggccctacc
     3241 tggtgatgag ccggatgacc ggcaccacct ggcggtccgc gatggacggc acgaccgacc
     3301 ggaacgcgct gctcgccctg gcccgcgaac tcggccgggt gctcggccgg ctgcacaggg
     3361 tgccgctgac cgggaacacc gtgctcaccc cccattccga ggtcttcccg gaactgctgc
     3421 gggaacgccg cgcggcgacc gtcgaggacc accgcgggtg gggctacctc tcgccccggc
     3481 tgctggaccg cctggaggac tggctgccgg acgtggacac gctgctggcc ggccgcgaac
     3541 cccggttcgt ccacggcgac ctgcacggga ccaacatctt cgtggacctg gccgcgaccg
     3601 aggtcaccgg gatcgtcgac ttcaccgacg tctatgcggg agactcccgc tacagcctgg
     3661 tgcaactgca tctcaacgcc ttccggggcg accgcgagat cctggccgcg ctgctcgacg
     3721 gggcgcagtg gaagcggacc gaggacttcg cccgcgaact gctcgccttc accttcctgc
     3781 acgacttcga ggtgttcgag gagaccccgc tggatctctc cggcttcacc gatccggagg
     3841 aactggcgca gttcctctgg gggccgccgg acaccgcccc cggcgcctga cgccccgggc
     3901 cgcccggcgc cgcccccggc ccccggcggc cgccgggcgg agccccgccc ggcggcccgg
     3961 gagccccggg cccgcgccga agcccgctgc tgcgagcccg gagcgggccg gccgacggcg
     4021 gtgcgggccc ggcggcggac gctcagcagc ggcgggcgtg aaaggccctg gcatcctcga
     4081 tcatctcctc cagggtgggt cggccgggct tccatcccag ctcggcaagg atcaacgagg
     4141 acagtcgcac gacgaagttc ttctggatcg cgcccgtgct ggaagcactc aacctcgaag
     4201 cgtgtggttg cggagccatc tagcaaccac acgaaacatg cgcaacgaac cgcgcaacga
     4261 acaacgccta gaactggccc tagatgagct gactcgtatc gttggtaaac ctagtttgac
     4321 cagcatgttt taactacgtt cggtgagctg tcaacggggc ctgtaacggc acaacgaacc
     4381 gtgcaacgag agtggccacg gatgccacca caggcactac aacggagttc gccacgtaca
     4441 tcaccacaac caccgattct ggcggtgagc tccccgatat tcagcggaaa tggcttggta
     4501 tcgaccaaga ttcgtagaac cccgtctcgt ctggctggta ttcaaaacgg gcgcaacgaa
     4561 acacgcaacg agacaggcat ggcccaaacc agaaaactag cgtctaccag gacttttacc
     4621 tgtccgaccc gttgcaacgg aaccccccac ggaacccccg cgacacccgc tccccaattg
     4681 cgttagaaca gcggtggatt gtcggcttcg ttgtgggcct tttgagccgc ttcctgttct
     4741 gccgcacgct ctttcctcgc ccgatagccg agtcgcttaa cggtgtccag atgcagcccg
     4801 aaatgtttgg ccgtttgcgg ccaagagtgg ccctcgtcgt cgtgataggc gcggatgcgt
     4861 tcgcggcgtg cagcctgctc ggcgagccac tcgctgcgtt cctgcgccac gagccggacg
     4921 acgtggcgtt cggatagtcc ggtgattcga gcgccttcgg cggcggtcac gcgccgcttt
     4981 ttgcggacag tcggctgccg gttgtagccg tcgctgtagc cgtcgctcat agcaatgcct
     5041 ccatggctga cgcggacttt gcgcgccgcg caactgtgct cgccgccgtg cgcgctgctg
     5101 cgcccttccg cgagatggcc gactggcgcg cactgagtgt ggcctcgtag accacgatcc
     5161 cgtccgccca aatgcgcgac ttggttgtga tccaacgcca aatgctgttg gcgatggcgc
     5221 ggacctcgct gtccggtagc ggtccgggac acacgtcgtt gcacgggaat tcggcgtttc
     5281 gcgcgtggca ctcggcatag atcgcgcggc cgagtccgtc cacgttccgg gtcggcaggt
     5341 agatccgcat gagggcggga cgataggccc acaacctgac ggaatcgaac agtgcgcaat
     5401 tccgccctag cggcgtcgga gccgctttgt acgtggtctg ctgacgccag cgcggcggtg
     5461 gcatgttcgc gccgagctcg gcctcgatgt ggctgagtgt gtagagatct gagtggagcc
     5521 attccgtttc ccaggcgatg tggccggggt ttttggtcat gaggcctgag taactgcggt
     5581 cgccgtcgac ggcgcgccga aggccttcgg cgcacgccgc catgtatgcg agcggcttac
     5641 gccgcgcgta ttcggtgcgt ggaacagggg cgttgagtgc ccacactgcg tgtgcgtggc
     5701 cgttggcgcg attgcccacg atcgcgttgg gcagcggatg ggacccccgg gcgctgagcg
     5761 ctcggagcgc tgcgtctgga tggtctacgt ccacgaccag caggtttgcc agcgctgttg
     5821 ggttcgcctc gatgtaccgg cggcctaggg ccgacgcgcg gctttggcgg tagatcccct
     5881 cgagcagatc gtcgcttgcc agcggccagt acggcagcca gagctgctca aattcgtcgg
     5941 cgacgtggct cacgcttggt agtagaccac gattaatcac cggtgtatgg tccgacacga
     6001 gctccaagtc agatatttcg ctgaggggcc accccacaac tgcacactcc cccgctctcc
     6061 cgtcgagccc tggtggtgga acaccagcga cagccgagca cccccaacca cctgtaccaa
     6121 ccaggaggaa cacatgcgtc gtttcgagga cgtttccggg ccgctgagag ccgctgtggc
     6181 ggccgtacac gccgccttag acccgttaga ccccctgccg cctgaatgcg cgggtacgag
     6241 ccacacagcg cccgaactta cggagctggt gggctcacct ggctttatgg cgtacgaatc
     6301 ggctgtgtgc gacctgttgg gcgaggtgag gtacgcgcta ctcacgctgg caagggcgac
     6361 agagccgccc caccgagccc gcacggccgc gcgcggtgtc aacaaccggg tgagtcgtgc
     6421 acaccagcag gtgttcgagg cttggctcga agtgcaggac atcgtggcga acgccgcccg
     6481 atgagccgcg ccttacgctg gctgccagcc gttcgcgggc tggttggtgc agcgcgtcga
     6541 gcggttagag gccctgcggt gttccaccac cgcagggcct cgcccttttt aaggctgaat
     6601 ttgcttgtct ccgaatccaa ctggcttgtc caagggtgta tctacgctta atccaaagtt
     6661 caaacgaggg gattacacat gaccaacttc gataacgttc tcggctcgat gatccccggg
     6721 ggccccccct cgaggtcgac ggtatcgata
//
