LOCUS       sequence-47813-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ChETA-TdTomato in cells lacking Cre (Cre-Off), for 
            optogenetic excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Saunders A, Johnson CA, Sabatini BL
  TITLE     Novel recombinant adeno-associated viruses for Cre activated and 
            inactivated transgene expression in neurons.
  JOURNAL   Front Neural Circuits. 2012 Jul 27;6:47. doi: 
            10.3389/fncir.2012.00047. eCollection 2012.
  PUBMED    22866029
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2012-07-27"; volume: "6:47. doi"; pages: "
            10.3389/fncir.2012.00047. eCollection 2012"
COMMENT     Sequence Label: pAAV-Ef1a-DO-ChETA-TdTomato-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..82)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(133..166)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(281..304)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(479..498)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     748..766
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcat aacttcgtat
       61 agcatacatt atacgaagtt attctttgca ccattctaaa gaataacagt gataatttct
      121 gggttaaggc aaataacttc gtataggata ctttatacga agttatgcta gcgctaccgg
      181 tcgccaccgc caccttactt gtacagctcg tccatgccgt acaggaacag gtggtggcgg
      241 ccctcggagc gctcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc
      301 agcttggtgt ccacgtagta gtagccgggc agttgcacgg gcttcttggc catgtagatg
      361 gtcttgaact ccaccaggta gtggccgccg tccttcagct tcagggcctg gtggatctcg
      421 cccttcagca cgccgtcgcg ggggtacagg cgctcggtgg aggcctccca gcccatggtc
      481 ttcttctgca ttacggggcc gtcggggggg aagttggtgc cgcgcatctt caccttgtag
      541 atcagcgtgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccagacc gccgtcctcg
      601 aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttctt gtaatcgggg
      661 atgtcggcgg ggtgcttcac gtacgccttg gagccgtaca tgaactgggg ggacaggatg
      721 tcccaggcga agggcagggg gccgcccttg gtcaccttca gcttggcggt ctgggtgccc
      781 tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgt
//
