LOCUS       sequence-478130-        6540 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses HA-tagged LpqZ in mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Lissner R, Franklin A, Benedict ST, Charitou V, Speer A, Knol J, 
            Jimenez C, Moynihan PJ, Nejentsev S, Kuijl C, Bitter W
  TITLE     A new role for lipoproteins LpqZ and FecB in orchestrating 
            mycobacterial cell envelope biogenesis.
  JOURNAL   mBio. 2025 Dec 12:e0211925. doi: 10.1128/mbio.02119-25.
  PUBMED    41384969
REFERENCE   2  (bases 1 to 6540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2025 
            Dec 12:e0211925. doi: 10.1128/mbio.02119-25."
COMMENT     Sequence Label: pSMT3-lpqZ-HA
FEATURES             Location/Qualifiers
     source          1..6540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1256..1282
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1331..1347)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1331..1347)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1344..1366)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1355..1371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1379..1385,1386..1403,1404..1409))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1424..1445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1562..1579)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1733..2321)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1813..1832)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6520..6536
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6521..6537
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttccgat gccggtgttg gcgccggtga ccacaacgac gcgcccgctt tgatcggggg
       61 acgtctgcgg ccgaccattt acgggtcttg ttgtcgttgg cggtcatggg ccgaacatac
      121 tcacccggat cggagggccg aggtcacggt cgaacgaggg gcatgacccg gtgcggggct
      181 tcttgcactc ggcataggcg agtgctaaga ataacgttgg cactcgcgac cggtgagtgc
      241 taggtcggga cggtgaggcc aggcccgtcg tcgctgcgag tggcagcgag gacaacttga
      301 gccgtccgtc gcgggcactg cgcccggcca gcgtaagtag cggggttgcc gtcacccggt
      361 gacccccgtt tcatccccga tccggaggaa tcacgctagc gtgaaaatcg gcaggctggc
      421 cgcgctgctg ctgacggcgg cgcttgtggt cgccggttgt gggggtggcg accgtcgcgg
      481 cgccgcggag ctggtggtcg gagccacggc cgactccgag tcgacgctgc tggccgacat
      541 ctacgtcgcg gccctgcggt catacggatt cccggcgcgg gccgcaaccg ccgccgaccc
      601 gatggcgaaa ttggactccg gtgactttac cgtggtcccc gccttcaccg gtgaggtctt
      661 gcgggcgcta cagccaagcg ccacggcgct ttctgacaaa gaggtctacc gggccatggt
      721 ctcggcgctg ccggaaggca ttgccgctgg tgactacgcc accgccgccg aagacaaacc
      781 cgcactgatg atcacccaag ccaccgccga cgggtggggg agcagcgaac tgtccgtgct
      841 ggcgcgccac tgcgacgggc tggtggtcgg gaaggtcggc ggcggcgcca ccccggcgat
      901 ggtgggcccg tgccggctgc ccgcggcggg tgaatttccc gacggtgccg cgatgttcgc
      961 ccggctgcgc gacggacggc tgaccgccgc atggaccagc accgccaacc ccgctgttcc
     1021 cgccggcctg gtcatgctga ccgacggcaa gcccgcgctg atcagagccg agaacgtggt
     1081 gccgctgtat cggcgcaacg cgctcaacga acgtcagctg ctggcggtca acgaggtggg
     1141 cggtgtgctg gacaccgcgg cgctggtgga catgcgccgc caggttgccg ggggtgcgga
     1201 cccgcaggcg gtggccgccg gttggctggg cgagcatccg ctggggcgtg gatcctatcc
     1261 gtatgatgtt cctgattatg cttaatctag agcggccgcc accgcggtgc gagccagatc
     1321 tggtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1381 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1441 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1501 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1561 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1621 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1681 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1741 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1801 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1861 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1921 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1981 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2041 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2101 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2161 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2221 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2281 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2341 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2401 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2461 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2521 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgac gtggccgacc
     2581 agcccgtcat cgtcaacgcc tgatccgcgg tgcggacagg ccgtgtcgcg accggccgtg
     2641 cggaattaag ccggcccgta ccctgtgaat agaggtccgc tgtgacacaa gaatccctgt
     2701 tacttctcga ccgtattgat tcggatgatt cctacgcgag cctgcggaac gaccaggaat
     2761 tctgggagcc gctggcccgc cgagccctgg aggagctcgg gctgccggtg ccgccggtgc
     2821 tgcgggtgcc cggcgagagc accaaccccg tactggtcgg cgagcccggc ccggtgatca
     2881 agctgttcgg cgagcactgg tgcggtccgg agagcctcgc gtcggagtcg gaggcgtacg
     2941 cggtcctggc ggacgccccg gtgccggtgc cccgcctcct cggccgcggc gagctgcggc
     3001 ccggcaccgg agcctggccg tggccctacc tggtgatgag ccggatgacc ggcaccacct
     3061 ggcggtccgc gatggacggc acgaccgacc ggaacgcgct gctcgccctg gcccgcgaac
     3121 tcggccgggt gctcggccgg ctgcacaggg tgccgctgac cgggaacacc gtgctcaccc
     3181 cccattccga ggtcttcccg gaactgctgc gggaacgccg cgcggcgacc gtcgaggacc
     3241 accgcgggtg gggctacctc tcgccccggc tgctggaccg cctggaggac tggctgccgg
     3301 acgtggacac gctgctggcc ggccgcgaac cccggttcgt ccacggcgac ctgcacggga
     3361 ccaacatctt cgtggacctg gccgcgaccg aggtcaccgg gatcgtcgac ttcaccgacg
     3421 tctatgcggg agactcccgc tacagcctgg tgcaactgca tctcaacgcc ttccggggcg
     3481 accgcgagat cctggccgcg ctgctcgacg gggcgcagtg gaagcggacc gaggacttcg
     3541 cccgcgaact gctcgccttc accttcctgc acgacttcga ggtgttcgag gagaccccgc
     3601 tggatctctc cggcttcacc gatccggagg aactggcgca gttcctctgg gggccgccgg
     3661 acaccgcccc cggcgcctga cgccccgggc cgcccggcgc cgcccccggc ccccggcggc
     3721 cgccgggcgg agccccgccc ggcggcccgg gagccccggg cccgcgccga agcccgctgc
     3781 tgcgagcccg gagcgggccg gccgacggcg gtgcgggccc ggcggcggac gctcagcagc
     3841 ggcgggcgtg aaaggccctg gcatcctcga tcatctcctc cagggtgggt cggccgggct
     3901 tccatcccag ctcggcaagg atcaacgagg acagtcgcac gacgaagttc ttctggatcg
     3961 cgcccgtgct ggaagcactc aacctcgaag cgtgtggttg cggagccatc tagcaaccac
     4021 acgaaacatg cgcaacgaac cgcgcaacga acaacgccta gaactggccc tagatgagct
     4081 gactcgtatc gttggtaaac ctagtttgac cagcatgttt taactacgtt cggtgagctg
     4141 tcaacggggc ctgtaacggc acaacgaacc gtgcaacgag agtggccacg gatgccacca
     4201 caggcactac aacggagttc gccacgtaca tcaccacaac caccgattct ggcggtgagc
     4261 tccccgatat tcagcggaaa tggcttggta tcgaccaaga ttcgtagaac cccgtctcgt
     4321 ctggctggta ttcaaaacgg gcgcaacgaa acacgcaacg agacaggcat ggcccaaacc
     4381 agaaaactag cgtctaccag gacttttacc tgtccgaccc gttgcaacgg aaccccccac
     4441 ggaacccccg cgacacccgc tccccaattg cgttagaaca gcggtggatt gtcggcttcg
     4501 ttgtgggcct tttgagccgc ttcctgttct gccgcacgct ctttcctcgc ccgatagccg
     4561 agtcgcttaa cggtgtccag atgcagcccg aaatgtttgg ccgtttgcgg ccaagagtgg
     4621 ccctcgtcgt cgtgataggc gcggatgcgt tcgcggcgtg cagcctgctc ggcgagccac
     4681 tcgctgcgtt cctgcgccac gagccggacg acgtggcgtt cggatagtcc ggtgattcga
     4741 gcgccttcgg cggcggtcac gcgccgcttt ttgcggacag tcggctgccg gttgtagccg
     4801 tcgctgtagc cgtcgctcat agcaatgcct ccatggctga cgcggacttt gcgcgccgcg
     4861 caactgtgct cgccgccgtg cgcgctgctg cgcccttccg cgagatggcc gactggcgcg
     4921 cactgagtgt ggcctcgtag accacgatcc cgtccgccca aatgcgcgac ttggttgtga
     4981 tccaacgcca aatgctgttg gcgatggcgc ggacctcgct gtccggtagc ggtccgggac
     5041 acacgtcgtt gcacgggaat tcggcgtttc gcgcgtggca ctcggcatag atcgcgcggc
     5101 cgagtccgtc cacgttccgg gtcggcaggt agatccgcat gagggcggga cgataggccc
     5161 acaacctgac ggaatcgaac agtgcgcaat tccgccctag cggcgtcgga gccgctttgt
     5221 acgtggtctg ctgacgccag cgcggcggtg gcatgttcgc gccgagctcg gcctcgatgt
     5281 ggctgagtgt gtagagatct gagtggagcc attccgtttc ccaggcgatg tggccggggt
     5341 ttttggtcat gaggcctgag taactgcggt cgccgtcgac ggcgcgccga aggccttcgg
     5401 cgcacgccgc catgtatgcg agcggcttac gccgcgcgta ttcggtgcgt ggaacagggg
     5461 cgttgagtgc ccacactgcg tgtgcgtggc cgttggcgcg attgcccacg atcgcgttgg
     5521 gcagcggatg ggacccccgg gcgctgagcg ctcggagcgc tgcgtctgga tggtctacgt
     5581 ccacgaccag caggtttgcc agcgctgttg ggttcgcctc gatgtaccgg cggcctaggg
     5641 ccgacgcgcg gctttggcgg tagatcccct cgagcagatc gtcgcttgcc agcggccagt
     5701 acggcagcca gagctgctca aattcgtcgg cgacgtggct cacgcttggt agtagaccac
     5761 gattaatcac cggtgtatgg tccgacacga gctccaagtc agatatttcg ctgaggggcc
     5821 accccacaac tgcacactcc cccgctctcc cgtcgagccc tggtggtgga acaccagcga
     5881 cagccgagca cccccaacca cctgtaccaa ccaggaggaa cacatgcgtc gtttcgagga
     5941 cgtttccggg ccgctgagag ccgctgtggc ggccgtacac gccgccttag acccgttaga
     6001 ccccctgccg cctgaatgcg cgggtacgag ccacacagcg cccgaactta cggagctggt
     6061 gggctcacct ggctttatgg cgtacgaatc ggctgtgtgc gacctgttgg gcgaggtgag
     6121 gtacgcgcta ctcacgctgg caagggcgac agagccgccc caccgagccc gcacggccgc
     6181 gcgcggtgtc aacaaccggg tgagtcgtgc acaccagcag gtgttcgagg cttggctcga
     6241 agtgcaggac atcgtggcga acgccgcccg atgagccgcg ccttacgctg gctgccagcc
     6301 gttcgcgggc tggttggtgc agcgcgtcga gcggttagag gccctgcggt gttccaccac
     6361 cgcagggcct cgcccttttt aaggctgaat ttgcttgtct ccgaatccaa ctggcttgtc
     6421 caagggtgta tctacgctta atccaaagtt caaacgaggg gattacacat gaccaacttc
     6481 gataacgttc tcggctcgat gatccccggg ggccccccct cgaggtcgac ggtatcgata
//
