LOCUS       sequence-47815-e         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of ChETA-tdTomato, for optogenetic 
            excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Saunders A, Johnson CA, Sabatini BL
  TITLE     Novel recombinant adeno-associated viruses for Cre activated and 
            inactivated transgene expression in neurons.
  JOURNAL   Front Neural Circuits. 2012 Jul 27;6:47. doi: 
            10.3389/fncir.2012.00047. eCollection 2012.
  PUBMED    22866029
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2012-07-27"; volume: "6:47. doi"; pages: "
            10.3389/fncir.2012.00047. eCollection 2012"
COMMENT     Sequence Label: pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..80)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(131..164)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      187..196
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcataa cttcgtatag
       61 catacattat acgaagttat tctttgcacc attctaaaga ataacagtga taatttctgg
      121 gttaaggcaa ataacttcgt ataggatact ttatacgaag ttatgctagc gctaccggtc
      181 gccaccgcca ccatggacta tggcggcgct ttgtctgccg tcggacgcga acttttgttc
      241 gttactaatc ctgtggtggt gaacgggtcc gtcctggtcc ctgaggatca atgttactgt
      301 gccggatgga ttgaatctcg cggcacgaac ggcgctcaga ccgcgtcaaa tgtcctgcag
      361 tggcttgcag caggattcag cattttgctg ctgatgttct atgcctacca aacctggaaa
      421 tctacatgcg gctgggagga gatctatgtg tgcgccattg aaatggttaa ggtgattctc
      481 gagttctttt ttgagtttaa gaatccctct atgctctacc ttgccacagg acaccgggtg
      541 cagtggctgc gctatgcaac ctggctgctc acttgtcctg tcatccttat ccgcctgagc
      601 aacctcaccg gcctgagcaa cgactacagc aggagaacca tgggactcct tgtctcagac
      661 atcgggacta tcgtgtgggg ggctaccagc gcc
//
