LOCUS       sequence-47816-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of ChETA-tdTomato, for optogenetic 
            excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Saunders A, Johnson CA, Sabatini BL
  TITLE     Novel recombinant adeno-associated viruses for Cre activated and 
            inactivated transgene expression in neurons.
  JOURNAL   Front Neural Circuits. 2012 Jul 27;6:47. doi: 
            10.3389/fncir.2012.00047. eCollection 2012.
  PUBMED    22866029
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2012-07-27"; volume: "6:47. doi"; pages: "
            10.3389/fncir.2012.00047. eCollection 2012"
COMMENT     Sequence Label: pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(27..60)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(111..144)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(258..281)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(456..475)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     725..743
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ccggatcctc tagagtcgac tccggaataa cttcgtatag gatactttat acgaagttat
       61 gcagaatggt agctggattg tagctgctat tagcaatatg aaacctctta ataacttcgt
      121 atagcataca ttatacgaag ttatggcgcg ccttatctag atttagccac cttacttgta
      181 cagctcgtcc atgccgtaca ggaacaggtg gtggcggccc tcggagcgct cgtactgttc
      241 cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc ttggtgtcca cgtagtagta
      301 gccgggcagt tgcacgggct tcttggccat gtagatggtc ttgaactcca ccaggtagtg
      361 gccgccgtcc ttcagcttca gggcctggtg gatctcgccc ttcagcacgc cgtcgcgggg
      421 gtacaggcgc tcggtggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc
      481 gggggggaag ttggtgccgc gcatcttcac cttgtagatc agcgtgccgt cctgcaggga
      541 ggagtcctgg gtcacggtca ccagaccgcc gtcctcgaag ttcatcacgc gctcccactt
      601 gaagccctcg gggaaggaca gcttcttgta atcggggatg tcggcggggt gcttcacgta
      661 cgccttggag ccgtacatga actgggggga caggatgtcc caggcgaagg gcagggggcc
      721 gcccttggtc accttcagct tggcggtctg ggtgccctcg
//
