LOCUS       sequence-47820-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by 
            alternative lox FAS sites), for optogenetic excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Saunders A, Johnson CA, Sabatini BL
  TITLE     Novel recombinant adeno-associated viruses for Cre activated and 
            inactivated transgene expression in neurons.
  JOURNAL   Front Neural Circuits. 2012 Jul 27;6:47. doi: 
            10.3389/fncir.2012.00047. eCollection 2012.
  PUBMED    22866029
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2012-07-27"; volume: "6:47. doi"; pages: "
            10.3389/fncir.2012.00047. eCollection 2012"
COMMENT     Sequence Label: pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      78..87
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgtgaggta cggatcctct agagtcgact ccggaacaac ttcgtatata cctttctata
       61 cgaagttgtg gcgcgccgcc accatggact atggcggcgc tttgtctgcc gtcggacgcg
      121 aacttttgtt cgttactaat cctgtggtgg tgaacgggtc cgtcctggtc cctgaggatc
      181 aatgttactg tgccggatgg attgaatctc gcggcacgaa cggcgctcag accgcgtcaa
      241 atgtcctgca gtggcttgca gcaggattca gcattttgct gctgatgttc tatgcctacc
      301 aaacctggaa atctacatgc ggctgggagg agatctatgt gtgcgccatt gaaatggtta
      361 aggtgattct cgagttcttt tttgagttta agaatccctc tatgctctac cttgccacag
      421 gacaccgggt gcagtggctg cgctatgcaa cctggctgct cacttgtcct gtcatcctta
      481 tccgcctgag caacctcacc ggcctgagca acgactacag caggagaacc atgggactcc
      541 ttgtctcaga catcgggact atcgtgtggg gggctaccag cgccatggca accggctatg
      601 ttaaagtcat cttcttttgt cttggattgt gctatggcgc gaacacattt tttcacgccg
      661 ccaaagcata tatcgagggt tatcatactg tgccaaaggg tcggtgccgc caggtcgtga
      721 ccggcatggc atggctgttt ttcgtgagct ggggtatgtt cccaattctc ttcattttgg
      781 ggcccgaagg ttttggcgtc ctgagcgtct atggctccac cgtaggtcac acgattattg
      841 atctgatgag taaaaattgt tgggggttgt tgggacacta cctgcgcgt
//
