LOCUS       sequence-47821-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Blount BA, Weenink T, Vasylechko S, Ellis T
  TITLE     Rational diversification of a promoter providing fine-tuned 
            expression and orthogonal regulation for synthetic biology.
  JOURNAL   PLoS One. 2012;7(3):e33279. Epub 2012 Mar 19.
  PUBMED    22442681
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33279. Epub 2012 Mar 19"
COMMENT     Sequence Label: pTAL6-BB
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             104..130
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaagtt tccgcctagg
       61 ttcaacaatg gcttcctccc ctccaaagaa aaagagaaag gttagttgga aggacgcaag
      121 tggttggtct agaatgcatg cggatcccat tcgtccgcgc aggccaagtc ctgcccgcga
      181 gcttctgccc ggaccccaac cggatagggt tcagccgact gcagatcgtg gggtgtctgc
      241 gcctgctggc agccctctgg atggcttgcc cgctcggcgg acggtgtccc ggacccggct
      301 gccatctccc cctgcgccct cacctgcgtt ctcggcgggc agcttcagcg atctgctccg
      361 tccgttcgat ccgtcgcttc ttgatacatc gcttcttgat tcgatgcctg ccgtcggcac
      421 gccgcataca gcggctgccc cagcagagtg ggatgaggcg caatcggctc tgcgtgcagc
      481 cgatgacccg ccacccaccg tgcgtgtcgc tgtcactgcc gcgcggccgc cgcgcgccaa
      541 gccggccccg cgacggcgtg ctgcgcaacc ctccgacgct tcgccggccg cgcaggtgga
      601 tctacgcacg ctcggctaca gtcagcagca gcaagagaag atcaaaccga aggtgcgttc
      661 gacagtggcg cagcaccacg aggcactggt gggccatggg tttacacacg cgcacatcgt
      721 tgcgctcagc caacacccgg cagcgttagg gaccgtcgct gtcacgtatc agcacataat
      781 cacggcgttg ccaga
//
