LOCUS       sequence-478241-        3165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lvl 1 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Duportet X, Wroblewska L, Guye P, Li Y, Eyquem J, Rieders J, 
            Rimchala T, Batt G, Weiss R
  TITLE     A platform for rapid prototyping of synthetic gene networks in 
            mammalian cells.
  JOURNAL   Nucleic Acids Res. 2014 Dec 1;42(21):13440-51. doi: 
            10.1093/nar/gku1082. Epub 2014 Nov 5.
  PUBMED    25378321
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1082"; journalName: "Nucleic Acids Res"; date: 
            "2014-12-1- 1"; volume: "42"; issue: "21"; pages: "13440-51"
COMMENT     Sequence Label: lvl1-S5S6 [backbone]
FEATURES             Location/Qualifiers
     source          1..3165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(269..857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(349..368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1028..1819,1820..1888))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1651..1670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1889..1993)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(2106..2149)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2152..2173
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    2174..2192
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2211..2222
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2229..2906
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      2951..3022
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3038..3065
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(3106..3123)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaggggtaa tggtctcacg ctggtagata agttgattta gccataaaat attgtttccg
       61 tgacccctta attaataggg ataacagggt aatatcttgc cctgcacgaa taccagcgac
      121 cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt gatgcggaag
      181 aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc acatctagga tctgccagga
      241 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      301 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      361 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      421 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      481 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      541 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      601 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      661 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      721 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      781 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      841 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
      901 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
      961 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1021 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1081 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1141 ctggccccag tgctgcaatg ataccgcgag aaccacgctc accggctcca gatttatcag
     1201 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1261 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1321 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1381 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1441 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1501 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     1561 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     1621 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     1681 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     1741 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     1801 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     1861 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaatcattt
     1921 atcaaggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     1981 taggggttcc gcgcacgaat tggccagcgc tgcattaccc tgttatccct aaccggttgc
     2041 ttgcaaaagc agtaattgga aagcactctc aaagaatccg gagtgagacc gtatcacgag
     2101 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg
     2161 gccgcttcta gagtccctat cagtgataga gatactgagc actactagag aaagaggaga
     2221 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
     2281 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
     2341 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
     2401 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
     2461 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
     2521 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
     2581 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
     2641 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
     2701 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
     2761 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
     2821 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
     2881 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag
     2941 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     3001 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     3061 ctgcgtttat atactagtag gatcctaact cgacgtgcag gcttcctcgc tcactgactc
     3121 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactc
//
