LOCUS       sequence-47825-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Zhu Y, Xu J, Heinemann SF
  TITLE     Two pathways of synaptic vesicle retrieval revealed by 
            single-vesicle imaging.
  JOURNAL   Neuron. 2009 Feb 12;61(3):397-411.
  PUBMED    19217377
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2009-02-12"; volume: "61(3)"; pages: "397-411"
COMMENT     Sequence Label: pcDNA3-SypHluorin 4x (S4x)
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      118..127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     676..692
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     676..692
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      706..715
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(745..773)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagctggcta gcgtttaaac gggccctcta gactcgagct caagcttgcc
      121 accatggacg tggtgaatca gctggtggct gggggtcagt tccgggtggt caaggagccc
      181 cttggcttcg tgaaggtgct gcagtgggtc tttgccatct tcgcctttgc tacgtgcggc
      241 agctacaccg gagagcttcg gctgagcgtg gagtgtgcca acaagacgga gagtgccctc
      301 aacatcgaag tcgaatttga gtacccattc aggctgcacc aagtgtactt tgatgcaccc
      361 tcctgcgtta aagggggcac taccaagatc ttcctagttg gtgactactc ctcctcggct
      421 gaattctttg tcaccgtggc tgtgtttgcc ttcctctact ccatgggggc cctggccacc
      481 tacatcttcc tgcagaacaa gtaccgagag aacaacaaag ggccaatgat ggacttcctg
      541 gccacagcag tgttcgcttt catgtggcta gttagctcat ccgcctgggc caaaggcctg
      601 tccgatgtga agatggccac tgacccagag aacattatca aggagatgcc tatgtgccgc
      661 cagacagatg gccgctctag aactagtgga tcccccatgg atctagccac catgggaagt
      721 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      781 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacat
//
