LOCUS       sequence-47829-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Seifert HS, Chen EY, So M, Heffron F
  TITLE     Shuttle mutagenesis: a method of transposon mutagenesis for 
            Saccharomyces cerevisiae.
  JOURNAL   Proc Natl Acad Sci U S A. 1986 Feb;83(3):735-9.
  PUBMED    3003748
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1986-02"; volume: "83(3)"; pages: "735-9"
COMMENT     Sequence Label: pHSS6
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(444..463)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tttgatcttt tctactgaac ggtgatcccc accggaattg cggccgcgga attctcatgt
       61 ttgacagctt atcatcgata agcttctaga gatcttccat acctaccagt tctgcgcctg
      121 cagcaatggc aacaacgttg cccggatccg cggccgcccg gggtgggcga agaactccag
      181 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
      241 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
      301 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
      361 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
      421 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
      481 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
      541 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc
      601 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
      661 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
      721 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
      781 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcc
//
