LOCUS       sequence-478305-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of TTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Sun X, Dyson HJ, Wright PE
  TITLE     Kinetic analysis of the multistep aggregation pathway of human 
            transthyretin.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Jul 3;115(27):E6201-E6208. doi: 
            10.1073/pnas.1807024115. Epub 2018 Jun 18.
  PUBMED    29915031
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1807024115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-07-3- 3"; volume: "115"; issue: "27"; pages: 
            "E6201-E6208"
COMMENT     Sequence Label: TTR C10S/S85C/F87A
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     175..194
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     282..301
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        282..300
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    301..325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             340..362
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(805..823)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa
       61 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
      121 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
      181 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
      241 tccggcgtag aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac tcactatagg
      301 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
      361 gatatacata tgggtcctac gggcaccggt gaatccaaga gtcctctgat ggtcaaagtt
      421 ctagatgctg tccgaggcag tcctgccatc aatgtggccg tgcatgtgtt cagaaaggct
      481 gctgatgaca cctgggagcc atttgcctct gggaaaacca gtgagtctgg agagctgcat
      541 gggctcacaa ctgaggagga atttgtagaa gggatataca aagtggaaat agacaccaaa
      601 tcttactgga aggcacttgg catctgccca gcccatgagc atgcagaggt ggtattcaca
      661 gccaacgact ccggcccccg ccgctacacc attgccgccc tgctgagccc ctactcctat
      721 tccaccacgg ctgtcgtcac caatcccaag gaatgagatc cggctgctac aaagcccgaa
      781 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      841 ctgaac
//
