LOCUS       sequence-47831-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Wong SS, Kotera I, Mills E, Suzuki H, Truong K
  TITLE     Split-intein mediated re-assembly of genetically encoded Ca(2+) 
            indicators.
  JOURNAL   Cell Calcium. 2011 Nov 29.
  PUBMED    22133610
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Calcium. 2011 Nov 29."
COMMENT     Sequence Label: NpuDnaEc-cGCaMP2
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        21..130
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     primer_bind     complement(343..364)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(604..623)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtaattccgg agttatccgg gacctttaat tcaacccaac acaatatatt atagttaaat
       61 aagacttatt atcaaatcat ttgtatatta attaaaatac tatactgtaa attacatttt
      121 atttacaatc aaaggagata taccatgggc ctgactagtg gatcccatat gatcaaaata
      181 gccacacgta aatatttagg caaacaaaat gtctatgaca ttggagttga gcgcgaccat
      241 aattttgcac tcaaaaatgg cttcatagct tctaattgtt tcaatggtac cgctagcatg
      301 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      361 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc cacctacggc
      421 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      481 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
      541 cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac catcttcttc
      601 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      661 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      721 ctggagtaca acacgcgtga ccaactgact gaagagcaga tcgcagaatt taaagaggct
      781 ttctccctat ttgacaagga cggggatggg acaataacaa ccaagggagc tggggacggt
      841 gatgcggtct ctggggcaga accccacaga agcaggacct gcaggacatg atcaatgaag
      901 tagatgccga cggtaat
//
