LOCUS       sequence-47836-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Meng X, Brodsky MH, Wolfe SA
  TITLE     A bacterial one-hybrid system for determining the DNA-binding 
            specificity of transcription factors.
  JOURNAL   Nat Biotechnol. 2005 Aug . 23(8):988-94.
  PUBMED    16041365
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2005 Aug . 23(8):988-94."
COMMENT     Sequence Label: pH3U3-mcs
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(65..70,71..88,89..95)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     105..127
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     124..140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     124..140
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             136..798
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 cctttcgtct tcaaacgcgt gtacacccgg gcggccgcgt gcactccgga ggcgcgccga
       61 attctttaca ctttatgctt ccggctcgta tgttgtgtcg accgagcgga taacaatttc
      121 acacaggaaa cagctatgac agagcagaaa gccctagtaa agcgtattac aaatgaaacc
      181 aagattcaga ttgcgatctc tttaaagggt ggtcccctag cgatagagca ctcgatcttc
      241 ccagaaaaag aggcagaagc agtagcagaa caggccacac aatcgcaagt gattaacgtc
      301 cacacaggta tagggtttct ggaccatatg atacatgctc tggccaagca ttccggctgg
      361 tcgctaatcg ttgagtgcat tggtgactta cacatagacg accatcacac cactgaagac
      421 tgcgggattg ctctcggtca agcttttaaa gaggccctag gggccgtgcg tggagtaaaa
      481 aggtttggat caggatttgc gcctttggat gaggcacttt ccagagcggt ggtagatctt
      541 tcgaacaggc cgtacgcagt tgtcgaactt ggtttgcaaa gggagaaagt aggagatctc
      601 tcttgcgaga tgatcccgca ttttcttgaa agctttgcag aggctagcag aattaccctc
      661 cacgttgatt gtctgcgagg caagaatgat catcaccgta gtgagagtgc gttcaaggct
      721 cttgcggttg ccataagaga agccacctcg cccaatggta ccaacgatgt tccctccacc
      781 aaaggtgttc ttatgtagtg a
//
